Thermo Fisher Scientific Pioneer™ MPS Accessory Owner's manual

Brand
Thermo Fisher Scientific
Model
Pioneer™ MPS Accessory
Type
Owner's manual
© 1999–2001 Applied Biosystems. All rights reserved.
Printed in the USA, 08/2001
Part Number PB601303 Rev. A
Preparing for Synthesis
1. Prepare an MPS Notebook and send it to
the system (see other side).
2. Print an MPS Calculation Sheet (MPS
User’s Guide).
3. Pack the columns (MPS User’s Guide).
4. Check and update the reagent resources
(MPS User’s Guide).
5. Install the amino acids in the Pioneer trans-
port system according to the Rack and Amino Acid Position diagram below (MPS User’s
Guide).
6. Prime the system and the MPS Accessory (MPS User’s Guide).
7. Install the columns on the MPS Accessory according to the Column Positions diagram
(MPS User’s Guide).
8. Empty the waste (MPS User’s Guide).
9. Start the synthesis (MPS User’s Guide).
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Column Positions
Pioneer racks move in counterclockwise direction
Rack position 1
Tube position 1
1
3
7
9
10
12
16
18
19
21
22
23
Leave two empty positions
between rack position 1 and
the red rack
4
6
13
15
Sampling
Position
Wash
Station
Rack and Amino Acid Positions
PmpsQrc.fm5 Page 1 Wednesday, September 5, 2001 7:08 PM
Pioneer™ MPS Accessory
Quick Reference Card
Using the MPS Notebook
1. Create or open an MPS Notebook in the Database window (MPS User’s Guide).
2. Enter the synthesis parameters (MPS User’s Guide):
•Select an MPS protocol.
•Select the Chemistry.
•Select the Scale.
•Select the Cycle.
•Select the Activator.
•Select the Final Cycle option.
3. Enter the sequence for each column (MPS User’s Guide, Section 2.2.3):
•Click the MPS column (a through p).
•Click the Sequence View button .
•Type the sequence, starting with the N-terminus.
•Type the sequence name in the Name text box.
•Click the MPS Notebook View button .
•Repeat the steps above for each MPS column (a through p).
4. Select the C-Terminus option (Acid or Amide) for each sequence.
5. Select the First AA option (On Support or Off Support) for each sequence.
6. Send the MPS Notebook to the system by clicking .
7. Prepare the System for synthesis (see other side).
For Research Use Only.
AB (Design), Applera, and Pioneer are trademarks of Applera Corporation or its subsidiaries in the U.S. and
certain other countries.
PmpsQrc.fm5 Page 2 Wednesday, September 5, 2001 7:08 PM
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Thermo Fisher Scientific Pioneer™ MPS Accessory Owner's manual

Brand
Thermo Fisher Scientific
Model
Pioneer™ MPS Accessory
Type
Owner's manual
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