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Thermo ScientificTM 384-Channel D.A.R.T. Transfer Tool Instruction Guide
Item# 650-08-DTT
384-Channel D.A.R.T. Transfer Tool packaged fully assembled with 24-pin configuration in right-handed orientation.
For use with 384 D.A.R.T. consumables and 384 channel pipetting heads.
This package includes the following items:
For applications assistance, technical support or questions on the use of this tool, contact
[email protected] or resource your local support team.
Handle
Plunger Button
Ejector
Plate
Pin Holder x2
24 pins for 384 style D.A.R.T.
Locking Pin
3/32nd
Allen
Wrench
Spring
Screw x4
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384-Channel D.A.R.T. Transfer Tool
Thank you for purchasing the 384-Channel D.A.R.T. Transfer Tool. This tool was designed to provide quick and easy
loading of the 384 style D.A.R.T. into a serial dilution magazine or other adapter/holder/rack. The tool is shipped with
the plunger button pressed down and locking pin inserted so the ejector plate is in a “locked” position to prevent
shipping damage to the pins. An Allen wrench is also supplied to reconfigure the pins as needed.
To remove the locking pin, press and hold the plunger button down while carefully pulling out the locking pin from the
handle; once the locking pin is removed, slowly release the plunger button up into its “home” position. DO NOT let the
plunger button snap up. Note: place the Allen wrench and locking pin in a secure location when not in use.
The tool comes preassembled in a 24 tip row-wise format, but can be switched to any tip configuration needed in either
column or row-wise formats. The tool is supplied in a right-handed orientation, which can be easily changed for left-
handed users. As long as the tool is handled with care, it can provide you with a lifetime of service.
Changing the Pin Configuration
The 384-Channel D.A.R.T. Transfer Tool is
supplied with 24 pins in a row-wise format. This
can be easily modified based on the column- or
row-wise set up needed for the application.
Store unused pins in a zip lock bag in the original
packaging supplied with the unit, or other
protective container, so that pins do not get lost or
damaged.
Column-wise format (16 pins) shown.
It is suggested to perform the disassembly and reassembly on a clean, dust-free work area so that you minimize the
chance of losing or damaging the components. You can lay the tool on one side on the bench, or if holding the tool, you
will need to carefully position the tool to remove and reassemble the components appropriately.
1. With one hand, hold the ejector plate in position against the handle with your index finger. Unscrew the plunger
button by turning it in a counter-clockwise direction, and place the plunger button to the side.
2. Slowly start turning the tool upside down so the spring drops out from the top of the handle, then place the
spring with the plunger button.
3. Tilt the tool right side up to allow the ejector plate to slide out of the handle, then place it with the other
components previously removed.
4. Turn the tool upside down so the pins are facing up.
While holding the pin holder in place with your index
finger, use the 3/32nd Allen wrench to loosen and
remove the two screws on either end of the pin holder
and place them to the side.
5. While still holding the pin holder in position, slowly turn
the tool so the pins are facing down, then begin sliding pin
holder/pins across ridge in handle to completely remove
from the tool.
Note: pins are not secured and are loose; gently place
these components down on the work area so that pins do
not drop out of the pin holder.
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6. Remove the selected number of pins from the pin holder to properly align in the D.A.R.T. magazine.
Pins should be installed flush
in the pin holder as shown.
The pins are best positioned
from the back of the tool where
less force is required in this
configuration to easily load tips.
7. Once the pins are correctly
configured, slide the pin
holder/pins back onto the
grooved slot on the handle
and secure the two screws on
each end.
Snug the screws into
position.
DO NOT OVERTIGHTEN!
8. Reassemble the remaining components
in reverse order:
a. Slide the ejector plate through
the handle and hold in position.
b. Place the spring onto the
ejector plate rod so the spring
is recessed into the handle.
c. Place the plunger button on the
top of the spring/rod and rotate
the button in a clock-wise
direction to secure.
Snug the plunger button onto
the metal rod of the ejector
plate.
DO NOT OVERTIGHTEN!
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9. Slowly push the plunger down and release up a couple times to ensure a smooth movement is present.
The transfer tool is now ready to use.
Changing the Handle Orientation (Right/Left Handed Users)
The 384-Channel D.A.R.T. Transfer Tool is supplied completely assembled in a right-handed orientation. This can be
switched for left handed users in a few simple steps.
Note: In order to load the D.A.R.T. easily without effort, pins should be in the closest position to the user
when lining up the tips in the tip magazine.
While holding the tool in your right hand, the pins are on the left side of the handle.
While holding the tool in your left hand, the pins are on the right side of the handle.
It is suggested to perform the disassembly and reassembly on a clean dust-free work area so that you minimize the
chance of losing or damaging the components.
1. Lay the tool on the bench. Unscrew the plunger button by turning it in a counter clockwise direction, and place
the plunger button to the side.
2. Remove the spring from the top of the handle and place it with the plunger button.
3. Slide the ejector plate out of the handle and place it with the other components previously removed.
4. Using the 3/32nd Allen wrench supplied with the tool, loosen and remove two screws on either end of the first
stainless steel pin holder without pins installed and place the screws and empty pin holder to the side.
5. Next, while holding the pin holder in place which houses the pins, loosen and remove two screws on either end
of the second stainless steel pin holder with pins installed and place the screws to the side.
Carefully slide the pin holder from the handle and reposition it on the opposite side on the handle.
CAUTION: pins are not secured and are loose, so you need to steadily switch pin holder/pins to the other side
of the handle in the opposite groove. Line up the groove on the pin holder with the groove on the handle and
slide the pin holder/pins into position.
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6. Reposition the pin holder/pins into the corresponding groove on the right side of the tool and secure the two
screws to hold components in place.
7. Next reposition the empty pin holder into the corresponding groove on the left side of the tool and secure the
two screws. Snug all screws into position. DO NOT OVERTIGHTEN!
8. Reassemble the remaining components in reverse order; slide the shaft/ejector plate through the handle, place
the spring in the top on the handle onto the ejector plate rod so it is recessed into the handle, place the plunger
button on the top of the spring/rod and rotate the button in a clock-wise direction to secure. Snug the plunger
button onto the shaft. DO NOT OVERTIGHTEN!
9. Slowly push the plunger down and release up a couple times to ensure a smooth movement is present.
The transfer tool is now ready to use in a left handed orientation.
Cleaning
To clean the 384-Channel D.A.R.T. Transfer Tool, select one of the following options using a damp lint-free cloth:
Alcohol based solution; IPA or Methanol
10% bleach solution
Wipe down the outer surfaces of the tool. Dry thoroughly and carefully position on lab bench for future use.
Note: The 384-Channel D.A.R.T. Transfer Tool is fully autoclavable at 121C for 15 minutes at @ 1 ATM (1 bar
pressure). Let the tool cool down at least 2 hours to room temperature before use.
CAUTION!
If the tool is autoclaved, do not touch the
DOME Label (Thermo Scientific) as the
adhesive may reactivate.
Ensure the DOME label is positioned
properly only if it moved out of alignment
on the handle.
Storage
If the tool needs to be removed from a crowded bench top to prevent
accidental damage, e.g. being dropped, store the 384-Channel
D.A.R.T. Transfer Tool in the original bubble bag and box supplied
and/or engage the locking pin so that the ejector plate is positioned
in its protective “locked” position. This prevents pin misalignment
or damage when the 384-Channel D.A.R.T. Transfer Tool is not in
use.
To insert locking pin: gently press the plunger button down and hold
so the ejector plate covers the pins as shown. Slide the locking pin
into the hole until it stops. Release the plunger button. The ejector
plate should remain stationary covering 80% of the pins.
The locking pin can be inserted in either side of the tool.
Grooves on handle / pin holder
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Ordering Information / Reference Guide
Thermo Scientific 384-Channel D.A.R.T. Transfer Tool
Item No. Description
650-08-384DTT DART Transfer Tool, 384-Channel
Thermo Scientific D.A.R.T. 384-Channel Serial Dilution Magazines
16/24-Channel for use with Thermo Scientific Versette
Item No. Description For use with DART series tips*
650-08-384SD 30 μl and 100 μl D.A.R.T., Column A1 / Row A1 5316, 5317, 5416, 5417, 5326, 5327
650-08-384SD Serial Dilution Magazine
16-Channel for use with Thermo Scientific Matrix PlateMate 2x2, PlateMate 2x3, PlateMate Plus and Hydra DT
(with X/Y stage)
Item No. Description For use with DART series tips*
500-10030 30 μl and 100 μl D.A.R.T., Column 1,2,13,14 5316, 5317, 5416, 5417, 5326, 5327
501-30052 30 μl and 100 μl D.A.R.T., Column 1,7,9,13,17,19 5316, 5317, 5416, 5417, 5326, 5327
500-10015 2x 12.5 μl tips, Column 1,2,13,14 5301, 5302, 5305
500-10020 2x 30 μl tips, Column 1,2,13,14 5311, 5312, 5315
500-10025 2x 125 μl tips, Column 1,2,13,14 5321, 5322, 5325
24-Channel for use with Thermo Scientific Matrix PlateMate 2x2, PlateMate 2x3, PlateMate Plus and Hydra DT
(with X/Y stage)
Item No. Description For use with DART series tips*
301-30052 30 μl and 100 μl D.A.R.T., Row A,E,I,M 5316, 5317, 5416, 5417, 5326, 5327
301-30022 30 μl and 100 μl D.A.R.T., Row A,B 5316, 5317, 5416, 5417, 5326, 5327
*Sterile, filtered D.A.R.T. style not compatible as filter inhibits proper fit onto pins.
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