Thermo Fisher Scientific EASY-Spray Series User guide

EASY-Spray Series

Ion Source

User Guide

60053-97275 Revision C February 2020

Accucore, EASY-nLC, EASY-Spray NG, Exactive, LCQ Fleet, nanoViper, PepMap, PepSwift, TSQ, and Viper

are trademarks; Unity is a registered service mark; and Acclaim, Dionex, EASY-Spray, LCQ, LTQ, Optima,

Orbitrap, Orbitrap Fusion, Thermo Scientific, Tribrid, UltiMate, and Xcalibur are registered trademarks of

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The following are registered trademarks in the United States and other countries: Microsoft and Windows are

registered trademarks of Microsoft Corporation.

The following are registered trademarks in the United States: Dino-Lite and DinoCapture are registered

trademarks of AnMo Electronics Corporation. IDEX is a registered trademark of IDEX Corporation.

Nanospray is a registered trademark of AB Sciex Pte. Ltd.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Rev A, April 2017; Rev B, June 2018: Rev C, February 2020

For Research Use Only. Not for use in diagnostic procedures.

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Thermo Scientific EASY-Spray Series Ion Source User Guide v

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Accessing Documentation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix

Viewing the Product Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Viewing Online User Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Compatible Mass Spectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Special Notices, Symbols, and Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Regulatory Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Equipment Ratings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Input and Output Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Advantages of Nanoelectrospray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Source Housing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Temperature Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Controller Power Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 2 Installing the EASY-Spray Series Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Connecting Power to the Ion Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Powering the Ion Source for an EASY-nLC 1200/MS System . . . . . . . . . . . . 10

Powering the Ion Source for Other nanoLC/MS Systems . . . . . . . . . . . . . . . 11

Attaching the Ion Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Adjusting the Emitter Tip Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Removing the EASY-Spray Series Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Chapter 3 Installing the Camera and LCD Monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Installing the USB Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Installing the Analog Camera and LCD Monitor . . . . . . . . . . . . . . . . . . . . . . . 19

Adjusting the Video Picture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Contents

vi EASY-Spray Series Ion Source User Guide Thermo Scientific

Chapter 4 Connecting the EASY-Spray Column Plumbing. . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Installing the EASY-Spray Column for the First Time. . . . . . . . . . . . . . . . . . . . 22

Setting the Column Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Using the nanoViper Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Examples of LC Connections to the EASY-Spray Column . . . . . . . . . . . . . . . . 26

Connecting the EASY-nLC 1200 to the EASY-Spray Column . . . . . . . . . . . 26

Configuring the EASY-nLC 1200 Plumbing for Two Columns . . . . . . . . . . 29

Connecting an RSLCnano System to the EASY-Spray Column. . . . . . . . . . . 29

Additional Resources. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Chapter 5 Configuring the Mass Spectrometer for NSI Mode . . . . . . . . . . . . . . . . . . . . . . . .33

Configuring the EASY-Spray NG Source’s NSI Parameters. . . . . . . . . . . . . . . . 33

Configuring the EASY-Spray Source’s NSI Parameters . . . . . . . . . . . . . . . . . . . 34

Chapter 6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Cleaning the EASY-Spray Column Emitter Tip . . . . . . . . . . . . . . . . . . . . . . . . 37

Replacing the Temperature Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Chapter 7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Chapter 8 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Spare Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Chapter A Contents of the Installation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49

Thermo Scientific EASY-Spray Series Ion Source User Guide vii

F

Figure 1. nanoLC/MS system (example) using the EASY-Spray ion source, camera,

and monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Figure 2. EASY-Spray NG ion source (P/N ES082) with the USB camera . . . . . . . . . . . . . 4

Figure 3. EASY-Spray ion source (P/N ES081) with the USB camera . . . . . . . . . . . . . . . . . 5

Figure 4. Temperature controller PCB located on the EASY-Spray NG

ion source (bottom) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Figure 5. Controller power supply assembly (shown with a European input plug) . . . . . . . 7

Figure 6. Power connection for the temperature controller PCB (bottom view) . . . . . . . . 10

Figure 7. Emitter positioning tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Figure 8. Gap positions in front of the MS ion transfer tube (side view) . . . . . . . . . . . . . . 14

Figure 9. USB camera installed on the EASY-Spray ion source . . . . . . . . . . . . . . . . . . . . 18

Figure 10. Analog camera installed on the EASY-Spray ion source . . . . . . . . . . . . . . . . . . . 19

Figure 11. EASY-Spray column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Figure 12. nanoViper fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Figure 13. Recommended plumbing locations on the venting Tee (nanoViper example). . 26

Figure 14. Tee holder secured to the bottom of the column holder . . . . . . . . . . . . . . . . . . 27

Figure 15. Example of a one-column setup with nanoViper fittings. . . . . . . . . . . . . . . . . . 27

Figure 16. Example of a two-column setup with nanoViper fittings and a Viper union . . . 28

Figure 17. EASY-nLC 1200 touch screen showing a two-column configuration . . . . . . . . 29

Figure 18. Example of a one-column setup for an RSLCnano system (direct injection) . . . 30

Figure 19. Example of a two-column setup for an RSLCnano system (preconcentration) . 31

Figure 20. Ion Source pane in the Tune window (example). . . . . . . . . . . . . . . . . . . . . . . . 34

Figure 21. NSI Source dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Figure 22. Wash cap installed over the column emitter tip . . . . . . . . . . . . . . . . . . . . . . . . 37

Figure 23. EASY-Spray ion source (bottom) with the new temperature controller PCB

and extra cover plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Figure 24. Temperature controller PCB (P/N ES233). . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Figure 25. EASY-Spray ion source (bottom) without the temperature controller PCB. . . . 39

Figures

viii EASY-Spray Series Ion Source User Guide Thermo Scientific

Thermo Scientific EASY-Spray Series Ion Source User Guide ix

P

Preface

The EASY-Spray Series Ion Source User Guide describes the hardware components, and

provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™

Series ion sources. Depending on the installed camera type, you view the ion source spray by

using the camera software or an LCD monitor that is placed on the mass spectrometer (MS).

Also, because this guide uses drawings of various connections and component parts to help

illustrate procedures, be sure to start from , no matter where it appears.

Contents

• Accessing Documentation

• Compatible Mass Spectrometers

• Special Notices, Symbols, and Cautions

• Safety Precautions

• Regulatory Compliance

•Contacting Us

Accessing Documentation

Thermo Scientific mass spectrometers (MS) include complete documentation.

•Viewing the Product Manuals

•Viewing Online User Documentation

For system requirements, refer to the release notes on the software DVD.

Preface

xEASY-Spray Series Ion Source User Guide Thermo Scientific

Viewing the Product Manuals

The Thermo Fisher Scientific service engineer installs the instrument control applications and

the instrument manuals on the data system computer.

To view the product manuals

From the Microsoft™ Windows™ taskbar, choose Start > All Apps (Windows 10) or

All Programs (Windows 7) > Thermo Instruments (or Thermo model) and so on,

where model is the specific MS.

Viewing Online User Documentation

Visit the Thermo Fisher Scientific website for product manuals and more.

To view user documentation from the Thermo Fisher Scientific website

1. Go to thermofisher.com.

2. Point to Services & Support and click Manuals on the left.

3. In the Refine Your Search box, search by the product name.

4. From the results list, click the title to open the document in your web browser, save it, or

print it.

To return to the document list, click the browser Back button.

Compatible Mass Spectrometers

Use the EASY-Spray Series ion source with the appropriate Thermo Scientific MS for

nanoelectrospray (commonly referred to as nanoES or nanospray™) analysis. Table 1 lists

several compatible MSs. For information about your specific instrument, refer to its manuals.

CAUTION If you connect the EASY-Spray Series ion source to another type of MS, you

might impair the safety protection provided by the equipment.

Table 1. Ion sources and compatible mass spectrometers

Ion source model Part Number Thermo Scientific mass spectrometer

EASY-Spray NG ES082 Orbitrap Tribrid Seriesa and TSQ™ Series II

aIn 2018, the name “Orbitrap Fusion™ Series” changed to “Orbitrap Tribrid Series.”

EASY-Spray ES081 Exactive™ Series, LCQ Fleet™, LTQ™ Series,

Orbitrap Series, and TSQ Series I

Preface

Thermo Scientific EASY-Spray Series Ion Source User Guide xi

Special Notices, Symbols, and Cautions

Make sure you understand the special notices, symbols, and caution labels in this guide. Most

of the special notices and cautions appear in boxes; those pertaining to safety also have

corresponding symbols. Some symbols are also marked on the ion source itself and can appear

in color or in black and white. For complete definitions, see Table 2.

Table 2. Notices, symbols, labels, and their meanings

Notice, symbol, or label Meaning

IMPORTANT Highlights information necessary to prevent damage to

software, loss of data, or invalid test results; or might contain

information that is critical for optimal performance of the

product.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Caution: Read the cautionary information associated with this

task.

Chemical hazard: Observe safe laboratory practices and

procedures when handling chemicals. Only work with volatile

chemicals under a fume or exhaust hood. Wear gloves and other

protective equipment, as appropriate, when handling toxic,

carcinogenic, mutagenic, corrosive, or irritant chemicals. Use

approved containers and proper procedures to dispose of waste

oil and when handling wet parts of the instrument.

Caution: Avoid Burns. Allow any heated components to cool

before touching them.

Risk of electric shock: This ion source uses voltages that can

cause electric shock and personal injury. Before removing the

ion source for servicing, shut down the MS and disconnect it

from line power.

Risk of eye injury: Eye injury can occur from splattered

chemicals, airborne particles, or sharp objects. Wear safety

glasses when handling chemicals or servicing the ion source.

Trip obstacle: Be aware of cords or other objects located on the

floor.

Preface

xii EASY-Spray Series Ion Source User Guide Thermo Scientific

Safety Precautions

Observe the following safety precautions when you operate or perform service on the ion

source.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure

full compliance with applicable North American and European regulations. The EASY-Spray

Series ion sources were tested as part of the Thermo Scientific MS system, which meets the

applicable requirements for electromagnetic compatibility (EMC) and product safety. For

additional regulatory information, refer to the MS manuals.

Unauthorized changes that you make to your system will void regulatory compliance and may

defeat the built-in protections for your instrument. Some examples of unauthorized changes

include using replacement parts or adding components, options, or peripherals that Thermo

Fisher Scientific has not qualified and authorized. Unauthorized changes can also result in

bodily injury and/or damage to your system and laboratory.

Ensure continued compliance with regulatory standards:

• Follow all installation instructions provided in the documentation that comes with your

system.

• Order replacement parts (as specified in the instrument manual) and additional

components, options, and peripherals directly from Thermo Fisher Scientific or an

authorized representative.

CAUTION Only perform service procedures in this manual. To avoid personal injury or

damage to the ion source, only the perform service procedures in this manual,

CAUTION The ion source must connect to a certified Thermo Scientific MS, which

supplies high voltage capable of delivering a maximum of 8 kV and 100 μA. If you

connect the source to another type of MS, you might impair the protection provided by

the equipment.

Preface

Thermo Scientific EASY-Spray Series Ion Source User Guide xiii

Contacting Us

Contact Email Telephone QR Code

U.S. Technical Support us.techsupport.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

U.S. Customer Service

and Sales

us.customer-support.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

Global Support To find global contact information or customize your request

1. Go to thermofisher.com.

2. Click Contact Us, select the country, and then select the type of support

you need.

3. At the prompt, type the product name.

4. Use the phone number or complete the online form.

To find product support, knowledge bases, and resources

Go to thermofisher.com/us/en/home/technical-resources.

To find product information

Go to thermofisher.com/us/en/home/brands/thermo-scientific.

Preface

xiv EASY-Spray Series Ion Source User Guide Thermo Scientific

Thermo Scientific EASY-Spray Series Ion Source User Guide 1

1

Introduction

The EASY-Spray Series ion source, shown in Figure 1, maintains excellent spray stability to

ensure efficient evaporation and ionization of the liquid samples—the key to achieving the

highest sensitivity at nano-flow rates.

Key benefits of the EASY-Spray Series are as follows:

• Simple and robust design for nano liquid chromatography (nanoLC)/MS integration

• Better separation with zero dead volume (ZDV) connections

• Increased productivity with the temperature-controlled EASY-Spray column

Contents

• Equipment Ratings

• Technical Specifications

• Input and Output Connections

• Environmental Conditions

• Advantages of Nanoelectrospray

• Functional Description

1 Introduction

Equipment Ratings

2EASY-Spray Series Ion Source User Guide Thermo Scientific

Figure 1. nanoLC/MS system (example) using the EASY-Spray ion source, camera, and monitor

Equipment Ratings

Easy Spray Ion Source input rating; 24 VDC, 20 W temperature control board; and

High-voltage 2.5 kVDC from Mass Spectrometer

Easy Spray Column input rating: 24 VDC for heater.

Technical Specifications

Input and Output Connections

• Easy Spray Ion Source, inputs: P-BUS, 24 VDC/20W, Mass Spectrometer High Voltage,

Mass Spectrometer Interlock detection;

• Easy Spray Ion Source, output: Heater (to Easy Spray Column)

Thermo Scientific

EASY-nLC™ 1200 instrument

Thermo Scientific mass spectrometer

with the EASY-Spray NG™ ion source

Item Dimensions Weight

Easy Spray Ion Spray Sources,

Models ES081 and ES082

18 cm high by 14 cm wide by 13 cm deep 2.6 Kg

Easy Spray Columns, all Models ES8xxA,

xx= 00, 01, 02, 03, 04, 05, 06, 10, 11, 12

37 mm high by 8 mm wide by 100 mm length 21 g

1 Introduction

Environmental Conditions

Thermo Scientific EASY-Spray Series Ion Source User Guide 3

Environmental Conditions

The Preinstallation Guide has the environmental conditions.

Advantages of Nanoelectrospray

The use of electrospray ionization (ESI) has evolved as a leading technique for generating

intact, gas-phase ions from thermally labile, polar analytes in solution. In this technique, an

emitter (a capillary tube or needle) induces ionization at a controlled distance from a counter

electrode. Direct current (dc) voltage is applied, either to the needle or to the solvent, to

produce a strong electrical field at the emitter tip. The electric field excites the ions in the

solution as they leave the emitter tip. This interaction results in electrohydrodynamic

disintegration of the fluid, generation of droplets, and formation of an aerosol jet.

Conventional ESI employs flow rates from 1 μL/min to 1 mL/min. Expediting desolvation

and droplet shrinkage often requires a drying gas, thermal heating, or both, due to the high

volume of liquid that exits the emitter. Nanospray ionization (NSI), also known as

nanoelectrospray ionization (nanoESI or NSI), is a form of ESI that employs low flow rates of

10 to 1000 nL/min. NSI (or nanoESI) generally does not require a drying gas or thermal

heating. Compared with ESI, NSI tolerates a wider range of liquid compositions including

pure water.

As you lower the flow rate, a lower volume of mobile phase passes through the emitter,

producing smaller aerosol droplets. This makes NSI more effective than conventional ESI at

concentrating the analyte at the emitter tip, which produces significant increases in sensitivity

as demonstrated by the signal response of the MS.

Functional Description

This section describes the following principal components of the EASY-Spray Series ion

source and their functions:

•Source Housing

•Temperature Controller PCB

•Controller Power Supply

Note The MS instrument control applications use the terms nanospray and NSI.

1 Introduction

Functional Description

4EASY-Spray Series Ion Source User Guide Thermo Scientific

Source Housing

The EASY-Spray Series ion source housing (Figure 2 or Figure 3) arrives assembled and is easy

to install on the appropriate MS (see Table 1). The ion source housing includes two locking

levers, two observation windows, a position-adjustable column holder, and a controller

printed circuit board (PCB).

Using the top and front observation windows, you can view the tip of the emitter positioning

tool while you move it into position. To enhance your view of the tool and then later of the

emitter, connect the video camera and LCD monitor (if applicable). For instructions, see

Chapter 3, “Installing the Camera and LCD Monitor.”

The column holder is mounted onto the front observation window and includes a position

control knob. Use the control knob to align the z axis (front-to-back) of the emitter

positioning tool (page 14) with the MS ion transfer tube. To align the tool’s x-axis

(side-to-side) and y-axis (up-and-down) positions, use the controls that are built into the

EASY-Spray Series housing. For instructions, see To adjust the emitter tip position.

Figure 2. EASY-Spray NG ion source (P/N ES082) with the USB camera

Locking lever (unlocked)

Column holder

Camera observation window

Source housing

Front observation window

1 Introduction

Functional Description

Thermo Scientific EASY-Spray Series Ion Source User Guide 5

Figure 3. EASY-Spray ion source (P/N ES081) with the USB camera

Source housing

Column holder

Camera observation window

Locking lever

Front observation window

1 Introduction

Functional Description

6EASY-Spray Series Ion Source User Guide Thermo Scientific

Temperature Controller PCB

The EASY-Spray Series ion source contains a temperature controller PCB that powers and

controls the heat generated for the connected EASY-Spray column. The temperature

controller PCB includes an input power socket, an output heater socket, a temperature

control knob, and an actual temperature display. The bottom panel for the EASY-Spray NG

ion source (Figure 4) is slightly different from that of the EASY-Spray ion source.

Figure 4. Temperature controller PCB located on the EASY-Spray NG ion source (bottom)

The controller power supply connects to the input power socket (labeled P-BUS), which is

located on the bottom right side of the ion source. The heater cable from the EASY-Spray

column connects to the heater socket, which is located on the front (labeled Heater).

The temperature control knob has an OFF setting and temperature settings in 5 °C

increments from 30 through 60 °C. As the column heats up, the actual temperature appears

on the display.

Tip If you have the Thermo Scientific EASY-nLC 1200 instrument and the EASY-Spray

P-Bus Temp Control Cablea (P/N 70005-63055), you can power the EASY-Spray Series

ion source by connecting the cable between the ion source and the LC instrument. This

connection also enables touch-screen control of the EASY-Spray column temperature.

For cable installation instructions, see To connect the EASY-Spray P-Bus Temp Control

Cable. For information about controlling the column temperature through the

EASY-nLC 1200 instrument’s touch screen or the Xcalibur™ Instrument Setup window,

refer to the LC instrument manuals.

aFor use with the EASY-nLC 1200 touch-screen application version 4.1 and later

Input power connection

Actual temperature display

Temperature controller PCB (not visible)

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Thermo Fisher Scientific EASY-Spray Series User guide

Thermo Fisher Scientific EASY-Spray Series User guide

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