Thermo Fisher Scientific UVPD Module User guide

UVPD Module

User Guide

For the Orbitrap Fusion Lumos Tribrid Mass Spectrometer

80011-97502 Revision A July 2017

Fusion and Lumos are trademarks, and Orbitrap, Orbitrap Fusion, Orbitrap Fusion Lumos, Thermo

Scientific, Tribrid, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.

The following are registered trademarks in the United States and other countries: Microsoft and Windows are

registered trademarks of Microsoft Corporation.

CryLaS is a registered trademark of CryLaS GmbH in Germany.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Rev A, July 2017

Software version: (Thermo) Foundation 3.1 SP4 and later, Xcalibur 4.1 and later, Tune 3.0 and later

For Research Use Only. Not for use in diagnostic procedures.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable North American and European regulations. Your system meets the applicable requirements in the

electromagnetic compatibility (EMC) and product safety standards described in this section.

Changes that you make to your system might void compliance with one or more of these regulatory standards. Changes

to your system include replacing a part or adding components, options, or peripherals not specifically authorized and

qualified by Thermo Fisher Scientific. To ensure continued compliance with the regulatory standards, order

replacement parts and additional components, options, and peripherals directly from Thermo Fisher Scientific or an

authorized representative.

Laser product safety

This device complies with the FDA Class 1 requirements in 21 CFR 1040.10. It also complies with the laser safety

standards IEC 60825-1: 2017 and ANSI Z316.1-2014.

Low Voltage Directive 2014/35/EU

This device complies with Low Voltage Directive 2014/35/EU and the harmonized safety standard IEC/EN/CSA/

UL 61010-1, 3rd Edition.

EMC Directive 2014/30/EU and other EMC test standards

This device was tested by TÜV Rheinland of North America and complies with the following EMC standards:

47 CFR 15, Subpart B, Class A: 2015 VCCI V-3/2015.04 IEC/EN 61000-4-4: 2004 + A1

AS/NZS CISPR 22: 2009 EN 55011: 2009 + A1 IEC/EN 61000-4-5: 2006

BSMI CNS 13438: 2006 IEC/EN 61326-1: 2013 IEC/EN 61000-4-6: 2009

ICES-003: 2014 IEC/EN 61000-3-2: 2006 + A1 + A2 IEC/EN 61000-4-8: 2010

IEC/CISPR 11: 2009 + A1 IEC/EN 61000-3-3: 2008 IEC/EN 61000-4-11: 2004

KN 61000-6-2 IEC/EN 61000-4-2: 2009

KN 61000-6-4 IEC/EN 61000-4-3: 2006 + A1 + A2

FCC Compliance Statement

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: This instrument must be used in the manner specified by Thermo Fisher

Scientific to ensure protections provided by the instrument are not impaired. Deviations from specified instructions on

the proper use of the instrument include changes to the system and part replacement. Accordingly, order replacement

parts from Thermo Fisher Scientific or one of its authorized representatives.

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

Thermo Scientific UVPD Module User Guide v

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Accessing Documentation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix

Special Notices, Symbols, and Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Ultraviolet Photodissociation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

UVPD Scan Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

UVPD Readback Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Chapter 2 UVPD Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Laser Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Controller Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Chapter 3 UVPD Laser Beam . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Laser Alignment Quality Score . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Laser Beam Movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Laser Beam Overlap with the Ion Clouds in the Ion Trap Chamber . . . . . . . . . 12

Chapter 4 UVPD Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

Laser Alignment Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Precursor Decay Rate Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Laser Alignment Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Running the UVPD Calibration Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Manually Aligning the Laser Beam . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Chapter 5 UVPD Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

Setting Up the MS/UVPD System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Optimizing the API Source Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Defining the Scan Parameters for the Experiment . . . . . . . . . . . . . . . . . . . . . . . 30

Acquiring Sample Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Contents

vi UVPD Module User Guide Thermo Scientific

Appendix A Laser Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Laser Safety Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Laser Safety Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Laser Head Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Thermo Scientific UVPD Module User Guide vii

F

Figure 1. Approximate location of the UVPD laser module inside the Orbitrap Fusion

Lumos MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Figure 2. UVPD scan parameters in the Tune application . . . . . . . . . . . . . . . . . . . . . . . . . 3

Figure 3. Laser module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Figure 4. Complementary pairs of micrometers (front view) . . . . . . . . . . . . . . . . . . . . . . . . 7

Figure 5. Mirror assemblies behind the laser beam protective housing . . . . . . . . . . . . . . . . 7

Figure 6. Micrometer locking thumbscrew and graduation markings . . . . . . . . . . . . . . . . . 8

Figure 7. Controller module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Figure 8. Example of optimal UVPD efficiency and AQS . . . . . . . . . . . . . . . . . . . . . . . . . 10

Figure 9. Laser beam paths after adjusting one or more micrometers . . . . . . . . . . . . . . . . 11

Figure 10. Laser beam (blue) overlap with an ion cloud (green) in the ion trap . . . . . . . . . . 12

Figure 11. Examples of optimal and poor UVPD efficiency (level laser beam through

the trap) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Figure 12. Examples of optimal and poor UVPD efficiency (angled laser beam through

the trap) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Figure 13. Laser Alignment calibration displays (percentage of precursor fragmentation

and AQS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Figure 14. Plot of the precursor decay rate in the ion trap’s low-pressure cell . . . . . . . . . . . 17

Figure 15. Plot of the UVPD reaction time for the caffeine precursor . . . . . . . . . . . . . . . . . 17

Figure 16. Plot of the UVPD alignment quality check . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Figure 17. Plot of the UVPD fragmentation efficiency check . . . . . . . . . . . . . . . . . . . . . . . 18

Figure 18. UVPD check calibration procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Figure 19. UVPD calibration procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Figure 20. Laser module compartment with door removed . . . . . . . . . . . . . . . . . . . . . . . . 21

Figure 21. Syringe pump settings box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Figure 22. Define Scan pane showing the scan parameters for optimizing the ion source. . . 27

Figure 23. RSD results for spray stability when optimizing the ion source parameters . . . . 28

Figure 24. Full MS scan spectrum of the enfuvirtide solution (profile mode) . . . . . . . . . . . 29

Figure 25. Define Scan pane showing the scan parameters for the UVPD experiment . . . . 30

Figure 26. MS2 scan spectrum of the enfuvirtide solution (profile mode) . . . . . . . . . . . . . . 31

Figure 27. Location of the laser safety label on the Orbitrap Fusion Lumos MS with the

UVPD option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Figure 28. Laser safety label for the Orbitrap Fusion Lumos MS with the UVPD option . . 34

Figure 29. Internal laser safety label (on and behind the laser beam protective housing) . . . 35

Figure 30. Laser head’s Class 4 laser safety label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Figures

viii UVPD Module User Guide Thermo Scientific

Thermo Scientific UVPD Module User Guide ix

P

Preface

The UVPD Module User Guide describes the primary components for the optional Thermo

Scientific™ ultraviolet photodissociation (UVPD) hardware module installed in the Thermo

Scientific Orbitrap Fusion Lumos™ (also known as Lumos™) Tribrid™ mass spectrometer

(MS). It also provides an example experiment and instructions for calibrating the alignment of

the invisible laser beam.

Contents

• Accessing Documentation

• Special Notices, Symbols, and Cautions

•Contacting Us

To suggest changes to the documentation or to the Help

Complete a brief survey about this document by clicking the button below.

Thank you in advance for your help.

Accessing Documentation

The Orbitrap Fusion Lumos MS includes complete documentation. For system requirements,

refer to the release notes on the software DVD.

To view the product manuals

From the Microsoft™ Windows™ taskbar, choose Start > All Programs > Thermo

Instruments > model x.x, and then open the applicable PDF file.

Preface

xUVPD Module User Guide Thermo Scientific

To view Help

Do the following as applicable:

• Thermo Tune instrument-control software: Click the Options icon, , and

choose Tu n e He l p .

• Thermo Xcalibur™ Method Editor: Choose an option from the Help menu (or press

the F1 key).

To view user documentation from the Thermo Fisher Scientific website

1. Go to thermofisher.com.

2. Point to Services & Support and click Manuals on the left.

3. In the Refine Your Search box, search by the product name.

4. From the results list, click the title to open the document in your web browser, save it, or

print it.

To return to the document list, click the browser Back button.

Special Notices, Symbols, and Cautions

Make sure you understand the special notices, symbols, and caution labels in this guide. Most

of the special notices and cautions appear in boxes; those pertaining to safety also have

corresponding symbols. Some symbols are also marked on the instrument itself and can

appear in color or in black and white. For complete definitions, see Table 1.

Table 1. Notices, symbols, labels, and their meanings (Sheet 1 of 2)

Notice, symbol, or label Meaning

IMPORTANT Highlights information necessary to prevent damage to

software, loss of data, or invalid test results; or might contain

information that is critical for optimal performance of the

product.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

Thermo Scientific UVPD Module User Guide xi

Caution: Read the cautionary information associated with this

task.

Chemical hazard: Observe safe laboratory practices and

procedures when handling chemicals. Only work with volatile

chemicals under a fume or exhaust hood. Wear gloves and other

protective equipment, as appropriate, when handling toxic,

carcinogenic, mutagenic, corrosive, or irritant chemicals. Use

approved containers and proper procedures to dispose of waste

oil and when handling wetted parts of the instrument.

Heavy object: The Orbitrap Fusion Series MS, excluding its

workbench, weighs over 227 kg (500 lb). Never try to detach

and move the instrument from its workbench; you can suffer

personal injury or damage the instrument.

Hot surface: Allow any heated components to cool before

touching them.

Risk of electric shock: This instrument uses voltages that can

cause electric shock and personal injury. Before servicing the

instrument, shut it down and disconnect it from line power.

While operating the instrument, keep covers on.

Risk of eye injury: Eye injury can occur from splattered

chemicals, airborne particles, or sharp objects. Wear safety

glasses when handling chemicals or servicing the instrument.

Risk of laser radiation: Failure to understand and comply with

laser cautions and operating instructions in this guide can result

in property damage or serious injuries to the user.

Sharp object: Avoid handling the tip of the syringe needle.

Table 1. Notices, symbols, labels, and their meanings (Sheet 2 of 2)

Notice, symbol, or label Meaning

Preface

xii UVPD Module User Guide Thermo Scientific

Contacting Us

Contact Email Telephone QR Codea

aYou can use your smartphone to scan a QR Code, which opens your email application or browser.

U.S. Technical Support us.techsupport.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

U.S. Customer Service

and Sales

us.customer-support.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

Global support To find global contact information or customize your request

1. Go to thermofisher.com.

2. Click Contact Us, select the country, and then select the type of support

you need.

3. At the prompt, type the product name.

4. Use the phone number or complete the online form.

To find product support, knowledge bases, and resources

Go to thermofisher.com/us/en/home/technical-resources.

To find product information

Go to thermofisher.com/us/en/home/brands/thermo-scientific.

Note To provide feedback for this document, go to surveymonkey.com/s/PQM6P62 or send an email message to

Technical Publications (techpubs-lcms@thermofisher.com).

Thermo Scientific UVPD Module User Guide 1

1

Introduction

The Orbitrap Fusion Lumos Tribrid MS is a member of the Thermo Scientific family of mass

spectrometers. The optional UVPD module assembly can come factory-installed with the

purchase of the Orbitrap Fusion Lumos MS, or a laser-safety-trained Thermo Fisher Scientific

field service engineer can install it at a later date as an upgrade.

An Orbitrap Fusion Lumos MS with UVPD capability incorporates an assembly consisting of

a laser module and a control module, both of which are located inside the instrument. For

information about these modules, see Chapter 2, “UVPD Module.” For information about

the invisible laser beam, see Chapter 3, “UVPD Laser Beam.” For laser safety information, see

Appendix A, “Laser Safety Information.”

For procedures regarding daily operation, maintenance, and system startup and shutdown,

refer to the Orbitrap Fusion Series Hardware Manual.

Contents

• Ultraviolet Photodissociation

• UVPD Scan Parameters

• UVPD Readback Measurements

Note The UVPD option is not available for the Thermo Scientific Orbitrap Fusion™ (also

known as Fusion™) Tribrid MS.

CAUTION Use of controls or adjustments or performance of procedures other than those

specified herein may result in hazardous radiation exposure.

Note The Glossary defines some of the terms used in this manual.

1 Introduction

Ultraviolet Photodissociation

2UVPD Module User Guide Thermo Scientific

Ultraviolet Photodissociation

The Orbitrap Fusion Lumos MS with UVPD capability incorporates an internal UVPD

module, which is located behind the right-side panel that connects to the ion trap chamber

manifold. Inside the module, a compact, solid-state laser head (CryLaS GmbH) generates a

pulsed 213 nm invisible laser beam that enters the ion trap, irradiating ions and producing

fragment ions. While collision-induced dissociation (CID) uses a vibrational excitation

mechanism to fragment molecular ions, UVPD uses an electronic excitation mechanism to

induce fragmentation.

Figure 1. Approximate location of the UVPD laser module inside the Orbitrap Fusion Lumos MS

As a fragmentation technique, UVPD offers several advantages:

• More informative fragmentation for a wide variety of compounds.

• More complete sequence coverage for intact proteins as well as unique structural

information for small molecules.

• Fast and direct cleavage of amide bonds at 5.82 eV per photon.

• Not limited by the precursor charge state, unlike ETD fragmentation.

• A wider array of sequence fragment types (a/x, b/y, and c/z) when activating peptidic

precursors.

• Simplicity. Activation time is the only parameter, which the MS sets automatically based

on the precursor molecular weight during method runs when the MS can determine the

precursor’s charge state.

UVPD compartment door

Laser Module

1 Introduction

UVPD Scan Parameters

Thermo Scientific UVPD Module User Guide 3

UVPD Scan Parameters

When you select the MS2 or MSn scan type in the Thermo Scientific Tune application’s

Define Scan pane (Figure 2), you can then select UVPD as the Activation Type and set the

UVPD activation time (in milliseconds). When you create a method in the Method Editor

application, you also have the option of using the molecular-weight-based UVPD activation

time that is determined by the Precursor Decay Rate Calibration.

For instructions on how to acquire data by using the Tune application, refer to the Orbitrap

Fusion Series Getting Started Guide. For descriptions of the parameters in the Define Scan

pane, refer to the Orbitrap Fusion Lumos Tune Help.

Figure 2. UVPD scan parameters in the Tune application

UVPD Readback Measurements

In addition to monitoring the overall system readback status in the Tune application, you can

monitor individual UVPD readback values on the By Board and By Function pages of the

Status pane. Table 2 lists the defaults for the UVPD readback values.

UVPD activation type

Table 2. Default values for the UVPD readbacks

Readback Typical value

Laser Status Ready, Standby, Not Connected

Laser On, Off

Laser Diode Voltage 1–6 V

Laser Temperaturea

aBy Board page only

10–60 °C

Laser Total Hoursa(Number of laser power-on hours)

Laser Pulse Counta0–500 Gigashots

1 Introduction

UVPD Readback Measurements

4UVPD Module User Guide Thermo Scientific

Thermo Scientific UVPD Module User Guide 5

2

UVPD Module

The UVPD module assembly for the Orbitrap Fusion Lumos MS has two principal

components—the laser module and the controller module. Installation of these modules

includes connections to three of the instrument printed circuit boards (PCBs):

• Carrier PCB—Provides USB communication between the MS and the laser controller.

• Distribution PCB—Powers the laser module.

• Source PCB—Provides the laser trigger signal during the UVPD scan functions.

Contents

•Laser Module

• Controller Module

Laser Module

The laser module (Figure 3) is located inside the MS on the right side. The user-accessible

laser compartment is behind the removable side door. The laser module assembly includes a

213 nm laser head, two adjustable mirror assemblies (each with two micrometers), and an

interlock switch assembly.

Note You cannot service the PCBs. If you need assistance, contact Thermo Fisher

Scientific Technical Support.

CAUTION Do not remove the laser beam protective housing. The protective housing

and safety interlock system prevent access to the invisible Class 4 laser beam. Exposure to

the beam can cause serious skin and eye injuries, including blindness.

2 UVPD Module

Laser Module

6UVPD Module User Guide Thermo Scientific

Figure 3. Laser module

Adjacent to each micrometer is a numbered label (1 to 4). Figure 4 shows the numbering and

the assigned “complementary pairs” of micrometers, which are 1-2 (top micrometers) and 3-4

(bottom micrometers). Complementary pairs work in the same plane of motion—that is,

movement that is vertical (pair 1-2) or horizontal (pair 3-4).

Protective housing

(shown as blue and

gold)

Mirror 2 (M2) assembly

(micrometers # 2 and #4)

Laser head with Class 4 laser safety labels

in yellow (see Figure 30 for label contents)

Mirror 1 (M1) assembly (micrometers #1 and #3)

Protective housing with Class 4 laser safety

label (see Figure 29 for label contents)

Label to record the fine-alignment micrometer positions

1

4

3

2

Interlock switch assembly (not shown)

2 UVPD Module

Laser Module

Thermo Scientific UVPD Module User Guide 7

Figure 4. Complementary pairs of micrometers (front view)

Figure 5 shows the mirror assemblies behind the protective housing. Each mirror assembly has

two micrometers that control the position of its mirror.

Figure 5. Mirror assemblies behind the laser beam protective housing

Top micrometers: pair 1–2

Bottom micrometers: pair 3–4

Micrometer number

4

2

3

1

Class 4 laser safety label (see Figure 29 for label contents)

M1 assembly Laser window into

the ion traps

M2 assembly

Interlock switch assembly

4

2

Laser head

1

3

2 UVPD Module

Controller Module

8UVPD Module User Guide Thermo Scientific

Figure 6 shows the micrometer and defines the graduation markings on its sleeve and thimble.

Figure 6. Micrometer locking thumbscrew and graduation markings

Controller Module

The controller module (Figure 7) is located inside the MS behind the back panel near the

distribution PCB. Enclosed within the controller module assembly is a power supply and a

controller unit, which controls the laser head.

Figure 7. Controller module

Thimble: Each mark is 0.005 mm (5 μm).Thumbscrew lock

Sleeve: Each top mark is 1 mm, and each bottom mark shows

half-millimeter positions (for example, 0.5 and 1.5 mm).

(End of 12 Vdc output cord

not shown)

Laser remote interlock connector

Controller unit (12 Vdc input)

Power supply (220 Vac input)

Laser head interface connector

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Thermo Fisher Scientific UVPD Module User guide

Thermo Fisher Scientific UVPD Module User guide

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