Thermo Fisher Scientific PFOA Analysis Kit Installation guide

Type
Installation guide
© 2011 Thermo Fisher Scientific Inc.
All rights reserved.
Preparing the LC system for the analysis of perfluorooctanoic acid (PFOA) requires replacing the wetted Teflon™
surfaces with comparable PEEK™ components and installing a PFOA trapping column.
Kit Contents The PFOA Analysis Kit (P/N 60057-62005) contains the following parts. After your local Thermo Fisher Scientific
field service engineer replaces the appropriate components of the LC system and installs the trapping column, use
the polypropylene vials to perform your analyses.
PFOA Analysis Kit Installation Guide
CAUTION Only a Thermo Fisher Scientific field service engineer can install the PFOA-free
vacuum chambers. Untrained personnel might damage the printed circuit boards (PCBs) and
cables inside the pump enclosure.
Contents
•Kit Contents
Replacing the Pump Parts
Replacing the Autosampler Parts
Installing the Trapping Column
Replacing the Solvent Reservoir Lines
•Trademarks
Description Quantity Part Number
Assemblies
Needle tube assembly, PEEK 1 60053-60073
Transfer tube assembly, PEEK 1 60053-60152
Tubing assembly, fast wash for Accela™ Open AS, PEEK 2 00950-01-00363
Fittings
Compression nut for 1/16 in. OD tubing, stainless steel 4 2522-1880
Ferrule, for 1/16 in. OD tubing, stainless steel, Valco™ 4 00101-18122
Super Flangeless™, ferrule system for 1/16 in. OD tubing, RoHS 18 00109-02-00030
Super Flangeless, nut for 1/16 in. OD tubing, PEEK, RoHS 18 00109-02-00031
Tubing
Tube, stainless steel, 0.005 in. ID ×1/16 in. OD, 10 cm length, precut 1 00301-01-00008
Tube, stainless steel, 0.005 in. ID ×1/16 in. OD, 5 cm length, precut 1 00301-01-00017
Tubing, PEEK, 0.040 in. ID ×1/16 in. OD 11.7 m (30 ft) N/A
Other
Column, BDS-Hypersil, C8, 5 μm particle size, 3 mm ID,
50 mm length (for trapping residual PFOA)
1 00109-01-00018
Filter, stainless steel, 20 μm particle size 4 00109-02-00032
Vacuum chamber, stented, PFOA free, RoHS 4 00108-01-00025
Vials, 600 μL, polypropylene 2 packages of
100 vials and
100 caps each
00109-99-00049
Revision B 60057-97040
2
Replacing the
Pump Parts
Replace these pump components:
Vacuum Degassing Chambers
Solvent Lines to the Proportioning Valves
Vacuum
Degassing
Chambers
The PFOA Analysis Kit contains four vacuum degassing chambers that have been treated to remove PFOA.
To replace the vacuum degassing chambers
1. Turn off the power to the pump.
2. Unplug the pump from line power.
3. To remove the pump chassis from the pump enclosure, do the following:
a. Disconnect the solvent reservoir lines from the inlet ports of the vacuum degasser.
b. Disconnect the tubing that connects the outlet of the pumps placeholder module or dynamic mixer to the
inlet of the autosampler’s heat exchanger.
c. Using a #2 Phillips head screwdriver, remove the screw that secures the metal plate covering the LED printed
circuit board and then remove the metal plate.
d. Disconnect the LED cable from the left door of the pump.
e. Using a #2 Phillips head screwdriver, remove the four screws that secure the pump chassis to the enclosure,
and then pull the chassis out of the enclosure.
4. Using a #2 Phillips head screwdriver, remove the two screws that secure the vacuum degasser assembly to the
pump chassis (see Figure 1).
Figure 1. Two screws that secure the vacuum degasser assembly to the pump chassis
CAUTION To prevent electrical shock, turn off the pump and disconnect it from line power
before pulling the pump chassis out of the enclosure.
CAUTION Observe precautions for handling electrostatic sensitive devices.
Note For more information about removing the pump chassis from the pump enclosure, refer to the
Accela 600 Pump and Accela 1250 Pump Hardware Manual.
3
5. To disconnect the vacuum chamber assembly from the vacuum degasser assembly, do the following:
a. Slide the vacuum degasser assembly away from the front panel of the pump chassis by approximately
0.75 cm (0.3 in.), until you feel the locking tabs on the bottom of the vacuum degasser assembly align with
the slots in the floor of the pump chassis.
Figure 2 shows the vacuum degasser assembly installed in the pump chassis.
Figure 3 shows the vacuum degasser assembly slid away from the front panel of the pump chassis.
Figure 4 on page 4 shows the locked and unlocked positions of the tabs on the bottom of the vacuum
degasser assembly.
Figure 2. Vacuum degasser assembly properly installed in the pump chassis (overhead view)
Figure 3. Vacuum degasser assembly slid away from the front panel of the pump chassis (overhead view)
Front panel of the pump chassis
Vacuum degasser assembly
properly installed
Front panel of the pump chassis
Vacuum degasser assembly
slid away from the front panel
of the pump chassis
4
Figure 4. Vacuum degasser assembly inside the pump chassis (side cutaway view)
(a) Locked position
(b) Unlocked position
(c) Angled position
Front panel of the
pump chassis
Vacuum chamber ports
In the locked position,
the locking tabs secure the
vacuum degasser assembly
to the pump chassis.
With the tabs in the
unlocked position, you can
lift the vacuum degasser
assembly up at a slight angle
from the pump chassis floor.
Front panel of the
pump chassis
Pump chassis floor
5
The locking tabs are now in the unlocked position as shown in Figure 4 (position b).
b. To provide access to the screws that secure the vacuum chamber assembly to the vacuum degasser assembly,
carefully lift the vacuum degasser assembly up at a slight angle as shown in Figure 4 (position c) and Figure 5.
Figure 5. Vacuum degasser assembly removed from the pump’s front panel
c. Using a #2 Phillips head screwdriver, remove the four screws (see Figure 5) that secure the vacuum chambers
to the vacuum degasser assembly.
Note To disconnect the vacuum chamber assembly from the vacuum degasser assembly, you do not need
to completely remove the vacuum degasser assembly from the pump.
If you want to remove the vacuum degasser assembly from the pump, you must disconnect the J1, J3,
and J5 cables and the vacuum hose connected to the vacuum pump.
Four screws that secure the
vacuum chambers to the
vacuum degasser assembly
Vacuum port openings on the
pump’s front panel
Placeholder module outlet
6
d. Pull off the vacuum hose connected to the right side of the top vacuum chamber and the vacuum hose
connected to the left side of the bottom vacuum chamber (see Figure 6).
Figure 6. Vacuum hose connections
6. Working from top to bottom, replace the vacuum chambers (see Figure 7) with the vacuum chambers from the
kit. Pull the vacuum hose off the left port of the top chamber and reconnect the hose to a new vacuum chamber.
Pull the vacuum hoses off the next vacuum chamber and reconnect the hoses to a new vacuum chamber, and
so on.
Figure 7. Vacuum chamber assembly
Vacuum pump
Vacuum hose connection
between the bottom vacuum chamber
and the vacuum pump
Vacuum hose connection
between the top vacuum chamber
and the vacuum pump
Top vacuum chamber
Bottom vacuum chamber
7
7. Reconnect the vacuum chamber assembly to the vacuum degasser assembly as follows:
a. Reconnect the vacuum hose from the vacuum pump to the top vacuum chamber.
b. Reconnect the vacuum hose from the vacuum sensor on the Control printed circuit board to the bottom
vacuum chamber.
Figure 8 shows the vacuum hose connections.
Figure 8. Vacuum hose connections
c. Using the four screws that you removed in step 5c on page 5, secure the vacuum chamber assembly to the
vacuum degasser assembly.
8. Realign the locking tabs on the bottom of the vacuum degasser assembly with the slots in the bottom of the
pump chassis (unlocked position shown in Figure 4 on page 4). If you put your hand beneath the pump chassis,
you can feel the tabs in the slots.
9. Slide the vacuum degasser assembly forward until the vacuum ports are immediately behind the openings in
the front panel and the locking tabs are in the locked position (see Figure 4 on page 4).
10. Using the two screws that you removed in step 4 on page 2, secure the vacuum degasser assembly to the pump
chassis.
11. To reconnect the pump chassis to the pump enclosure, do the following:
a. Push the pump chassis back into the pump enclosure.
b. Using the four screws that you removed in step 3e on page 2, secure the chassis to the enclosure.
c. Reconnect the LED cable.
d. Using the screw that you removed in step 3c on page 2, secure the LED cover plate to the left door of the
pump.
Connection between the vacuum pump
and the top vacuum chamber
Connection between the vacuum sensor
and the bottom vacuum chamber
Vacuum sensor on the
Control PCB
8
12. Reconnect the outlet of the placeholder module or the dynamic mixer to the inlet of the autosamplers heat
exchanger.
Solvent Lines
to the
Proportioning
Valves
The solvent lines that connect the vacuum degasser outlets to the proportioning valves are made of Teflon. Replace
these solvent lines with the PEEK tubing and Super Flangeless fittings from the PFOA Analysis Kit.
To replace the solvent lines that connect the degasser outlet ports to the proportioning valves
1. Cut the new tubing to the following lengths:
2. Using the Super Flangeless fittings from the PFOA Analysis Kit, connect the solvent lines to the vacuum degasser
outlets and the proportioning valves.
Replacing the
Autosampler
Parts
Depending on the autosampler model, replace these parts:
For the Accela Autosampler, replace the transfer tubing assembly, the needle tubing assembly, and the wash
solvent line connected to the left side of the syringe valve. For instructions about replacing the transfer tubing
assembly and the needle tubing assembly, refer to these topics in the Maintenance chapter of the Accela
Autosampler Hardware Manual: “Replacing the Transfer Tubing” and “Replacing the Needle Tubing Assembly.
For the Accela Open Autosampler with a fast wash station, replace the two wash solvent line assemblies (see
page 9). Each PFOA-free wash solvent line assembly consists of a length of PEEK tubing with compression
fittings and flared ends and a shutoff valve.
To replace the wash solvent line for the Accela Autosampler
1. Remove the bottle cap from the wash bottle.
2. Remove the filter from the end of the wash solvent line, and then pull the wash solvent line out of the wash
bottle cap.
3. Clean the wash bottle and fill it with fresh solvent.
4. Cut a length of PEEK tubing (from the kit) approximately 123 cm (4 ft) long.
5. Slide one end of the PEEK tubing through the wash bottle cap.
6. Screw the wash bottle cap onto the wash bottle.
7. Install a Super Flangeless fitting onto the free end of the wash bottle line, and then connect the Super Flangeless
fitting to the left side of the autosampler syringe valve.
IMPORTANT Do not reconnect the solvent reservoir lines to the inlet ports of the vacuum degasser. The solvent
reservoir lines are made of Teflon. Replace these lines as described in “Replacing the Solvent Reservoir Lines on
page 12.
Solvent line Length
A20cm
B22cm
C28cm
D33cm
CAUTION To prevent personal injury caused by skin contact or eye contact with hazardous
solvents, wear the appropriate safety gear when disposing of solvents.
Note The wash bottle line does not require a solvent filter.
9
To replace the wash lines for the Accela Open Autosampler (fast wash station configuration)
1. Remove each wash solvent line assembly as follows:
a. Turn the shutoff valve connected to the wash bottle spigot to the Closed position (see Figure 9).
Figure 9. Shutoff valve in the Closed position
b. Unscrew the other end of the wash line, which is connected to the wash station pump (see Figure 10).
Figure 10. Fast wash station
c. Drain the solvent in the wash line into an appropriate waste container.
d. Unscrew and remove the end of the wash line that is connected to the shutoff valve.
e. Empty the contents of the wash bottle into an appropriate waste container.
f. Loosen the nut that secures the shutoff nut to the wash bottle spigot, and then remove the shutoff valve from
the wash bottle.
Wash bottle
Closed
position
Fast wash station
Wash lines
Wash station pumps
10
2. Install each new shutoff valve as follows:
a. Slide the nut, washer, and ferrule onto the wash bottle spigot using the orientation shown in Figure 11.
Figure 11. Shutoff valve installation
b. Insert the shutoff valve body into the nut, and then tighten the nut.
c. Screw one end of the new wash line into the free end of the shutoff valve.
d. Screw the other end of the new wash line into the wash station pump.
e. Fill the wash bottle with the appropriate solvent.
f. Open the shutoff valve by placing its lever parallel to the wash line as shown in Figure 12.
Figure 12. Shutoff valve in the Open position
Ferrule
Washer
Nut Spigot
Shutoff valve body
Lever in the Closed position
(perpendicular to the wash line)
Wash line
Lever in the Open position
(parallel to the wash line)
Wash line
11
Installing the
Trapping
Column
To capture residual PFOA, install a trapping column in the flow path between the outlet of the pumps liquid
displacement assembly (LDA) and the outlet of the pumps placeholder module or dynamic mixer.
To install the trapping column
1. Using a 1/4 in. open-ended wrench, remove the static mixer from the pump as follows:
Remove the fitting connected to the LDA outlet.
Remove the fitting connected to the inlet of the pumps placeholder module or dynamic mixer.
2. Using two of the supplied two-piece compression fittings (see Figure 13) and the precut, 5 cm length of stainless
steel tubing, connect the inlet of the trapping column to the LDA outlet.
Figure 13. Two-piece, stainless steel compression fitting
3. Using two of the supplied two-piece compression fittings and the precut, 10 cm length of stainless steel tubing,
connect the outlet of the trapping column to the inlet of the placeholder module or dynamic mixer.
Figure 14 shows the trapping column installed between the LDA outlet and the placeholder module inlet.
Figure 14. Trapping column installed between the LDA and the placeholder module
Note Use the supplied compression fittings to make the plumbing connections. Using other fittings might cause
leaks.
Note The trapping column provides adequate mixing.
Ferrule
(P/N 00101-18122)
Nut
(P/N 2522-1880)
LDA outlet Trapping column
5 cm length of tubing 10 cm length of tubing
Placeholder module inlet
12
Replacing the
Solvent
Reservoir
Lines
The solvent reservoir lines are made of Teflon. Use the Super Flangeless fittings and the PEEK tubing from the kit
to replace the solvent reservoir lines.
To replace the solvent reservoir lines
1. Remove the Teflon solvent reservoir lines from the solvent reservoir bottles.
2. Clean the solvent reservoir bottles and fill them with fresh solvent.
3. Cut four lengths of 0.04 in. ID PEEK tubing, approximately 1.5 m (5 ft) each.
The appropriate length for the solvent reservoir lines depends on the distance between the solvent reservoir
bottles and the inlet ports of the pumps vacuum degasser.
4. Remove the fittings from the solvent filters found in the kit.
5. Install each solvent reservoir line (see Figure 15) as follows:
a. Insert one end of the PEEK tubing through the solvent reservoir bottle cap, and then install a Super
Flangeless fitting on this end of the tubing.
b. Press the end of the tubing into the solvent filter port, and then screw the Super Flangeless fitting into the
solvent filter.
c. Screw the bottle cap onto the solvent reservoir bottle.
d. Install a Super Flangeless fitting onto the free end of the solvent reservoir line.
Figure 15. Solvent reservoir cap assembly with the solvent line assembly
6. Place the solvent reservoir bottle in the solvent platform.
7. Route the tubing through the clips located on the left side of the front panel of each Accela module.
8. Connect the solvent reservoir line to the appropriate inlet port of the pumps vacuum degasser.
Trademarks The following are registered trademarks in the United States and other countries: Teflon is a registered trademark
of E.I. du Pont de Nemours and Company. Swagelok is a registered trademark of Crawford Fitting Company.
Valco is a registered trademark of Valco Instruments Co. Inc. and VICI AG.
PEEK is a trademark of Victrex plc. Super Flangeless is a trademark of IDEX Health & Science, LLC.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Note The solvent filters from the kit come with a fitting for 1/8 in. OD tubing.
1/16 in. OD PEEK tubing
Solvent reservoir bottle cap
Solvent reservoir filter
(20 μm particle size,
stainless steel)
Super Flangeless fittings
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Thermo Fisher Scientific PFOA Analysis Kit Installation guide

Type
Installation guide

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