Attune™ Flow Cytometry Maintenance and Troubleshooting Guide
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Contents
About this guide ............................................................................................................... 5
Safety information ...................................................................................................................................................... 6
Flow Cytometer maintenance .......................................................................................... 7
Daily maintenance ...................................................................................................................................................... 8
Weekly maintenance ................................................................................................................................................ 12
Monthly maintenance .............................................................................................................................................. 13
Decontaminate system ............................................................................................................................................. 15
Replace focusing fluid filters ................................................................................................................................... 18
Replace the cytometer sample syringe .................................................................................................................. 19
Replace the SIP tube ................................................................................................................................................. 21
Autosampler maintenance ............................................................................................. 22
Daily maintenance – Autosampler ......................................................................................................................... 23
Decontaminate system ............................................................................................................................................. 25
Replace the Attune™ Auto Sampler syringe ......................................................................................................... 29
Replace the CytKick™/CytKick™ Max Autosampler syringe ............................................................................. 33
Calibrate the autosampler ....................................................................................................................................... 35
Prepare the autosampler for shipment .................................................................................................................. 36
Attune™ External Fluid Supply (EFS) maintenance ......................................................... 38
Instrument functions ..................................................................................................... 45
Startup ........................................................................................................................................................................ 46
Rinse ........................................................................................................................................................................... 47
Sanitize SIP ................................................................................................................................................................ 47
Unclog ........................................................................................................................................................................ 48
De-bubble .................................................................................................................................................................. 49
Shutdown................................................................................................................................................................... 50
Deep Clean ................................................................................................................................................................ 53
Troubleshooting ............................................................................................................. 55
Cytometer troubleshooting ..................................................................................................................................... 56
Performance tracking troubleshooting .................................................................................................................. 63
Sample troubleshooting ........................................................................................................................................... 65
Autosampler troubleshooting ................................................................................................................................. 67
Attune™ External Fluid Supply (EFS) troubleshooting ....................................................................................... 70