Roche X-tremeGENE 9 DNA Transfection Reagent Reference guide

Brand: Roche

Model: X-tremeGENE 9 DNA Transfection Reagent

Type: Reference guide

For life science research only. Not for use in diagnostic procedures.

X-TREMEGENE is a trademark of Roche.

Transfection procedureCell preparation for transfection

Plate cells approx. 24 hours before transfection

making sure cells are at optimal concentration

(70 – 90 % confluency).

Steps prior to transfection

Allow X-tremeGENE 9 DNA Transfection Reagent,

DNA and diluent (Opti:MEM I Reduced

Serum Medium or serum-free medium) to warm

to +15°C to +25°C, and vortex gently.

A detailed protocol is available in the online instructions for use at www.instructions.roche.com

For cell-type specific protocols, visit www.x-tremegene.roche.com. View the many successfully

used cell-type-specific transfection conditions in our customer feedback database.

Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

06516092001



0911

X-tremeGENE 9 DNA Transfection Reagent

Quick Protocol

For products with Catalog Numbers:

06 366 511 001 | 06 365 779 001 | 06 365 787 001 | 06 365 809 001

Volumes of X-tremeGENE 9 DNA Transfection Reagent and amounts of DNA for various ratios

Ratio

Transfection Reagent : DNA

Minimal transfection

complex volume

Volume for a whole

96-well plate

Serum-free medium to a final volume of

100 µl 500 µl

3 : 1

X-tremeGENE 9 Transfection Reagent

3 µl 15 µl

DNA

1 µg 5 µg

6 : 1

X-tremeGENE 9 Transfection Reagent

6 µl 30 µl

DNA

1 µg 5 µg

6 : 2

X-tremeGENE 9 Transfection Reagent

6 µl 30 µl

DNA

2 µg 10 µg

• Add transfection complex to the cells in a

dropwise manner.

• Gently shake or swirl the wells or ﬂ asks to

ensure even distribution over the entire plate.

Incubate cells for 18 – 72 hours before

measuring protein expression.

• Place diluent in a sterile tube.

• Add X-tremeGENE 9 DNA Transfection

Reagent to the diluent.

• Add plasmid DNA. Pipet gently to mix.

Incubate for 15 min at +15°C to +25°C.

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Roche X-tremeGENE 9 DNA Transfection Reagent Reference guide

Roche X-tremeGENE 9 DNA Transfection Reagent Reference guide

Roche X-tremeGENE 9 DNA Transfection Reagent Reference guide

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