Roche X-tremeGENE HP DNA Transfection Reagent Reference guide

Type
Reference guide
Add transfection complex to the cells in a
dropwise manner.
Gently shake or swirl the wells or fl asks to
ensure even distribution over the entire plate.
Incubate cells for 18 72 hours before
measuring protein expression.
Place diluent in a sterile tube.
Add plasmid DNA. Pipet gently to mix.
Add X-tremeGENE HP DNA Transfection
Reagent to the diluted DNA.
Incubate for 15 min at +15°C to +25°C.
For life science research only. Not for use in diagnostic procedures.
X-TREMEGENE is a trademark of Roche.
Transfection procedureCell preparation for transfection
Plate cells approx. 24 hours before transfection
making sure cells are at optimal concentration
(70 – 90 % confluency).
Steps prior to transfection
Allow X-tremeGENE HP DNA Transfection Reagent,
DNA and diluent (Opti:MEM I Reduced
Serum Medium or serum-free medium) to warm
to +15°C to +25°C, and vortex gently.
A detailed protocol is available in the online instructions for use at www.instructions.roche.com
For cell-type specific protocols, visit www.x-tremegene.roche.com. View the many successfully
used cell-type-specific transfection conditions in our customer feedback database.
Roche Diagnostics GmbH
Sandhofer Straße 116
68305 Mannheim
Germany
© 2011 Roche Diagnostics.
All rights reserved.
06516084001
0911
X-tremeGENE HP DNA Transfection Reagent
Quick Protocol
For products with Catalog Numbers:
06 365 752 001 | 06 366 244 001 | 06 366 236 001 | 06 366 546 001
Volumes of X-tremeGENE HP DNA Transfection Reagent and amounts of DNA for various ratios
Ratio
Transfection Reagent : DNA
Minimal transfection
complex volume
Volume for a whole
96-well plate
Serum-free medium to a final volume of
100 µl 500 µl
1 : 1
X-tremeGENE HP Transfection Reagent
1 µl 5 µl
DNA
1 µg 5 µg
2 : 1
X-tremeGENE HP Transfection Reagent
2 µl 10 µl
DNA
1 µg 5 µg
3 : 1
X-tremeGENE HP Transfection Reagent
3 µl 15 µl
DNA
1 µg 5 µg
A C
B
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Roche X-tremeGENE HP DNA Transfection Reagent Reference guide

Type
Reference guide

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