Thermo Fisher Scientific E-Base Electrophoresis User guide

Brand: Thermo Fisher Scientific

Model: E-Base Electrophoresis

Type: User guide

USER GUIDE

For Research Use Only. Not for use in diagnostic procedures.

E-Base™ Electrophoresis Device

Catalog Numbers EB-M03 and EB-D03

Publication Part Number 25-0667

Publication Number MAN0002597

Revision A

For Research Use Only. Not for use in diagnostic procedures.

Information in this document is subject to change without notice.

DISCLAIMER

LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL

WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING

BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR

PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT

SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN

CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR

SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES

IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT

LIMITED TO THE USE THEREOF.

IMPORTANT LICENSING INFORMATION

These products may be covered by one or more Limited Use Label Licenses. By use of

these products, you accept the terms and conditions of all applicable Limited Use Label

Licenses.

TRADEMARKS

Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

Contents

Overview........................................................................................................ 4

Instructions for Use ...................................................................................... 7

E-Base™ Quick Reference Guide ............................................................... 12

Troubleshooting .......................................................................................... 14

Appendix............................................................................ 16

Explanation of Symbols and Warnings ................................................... 16

Saftey ............................................................................................................ 17

Explication des symboles et des avertissements .................................... 18

Sécurité ......................................................................................................... 19

Accessory Products .................................................................................... 20

Technical Support ....................................................................................... 21

Overview

Introduction

The E-Base™ device is an easy-to-use, programmable,

automated device combining a base and power supply

to simplify electrophoresis of pre-cast E-PAGE™ 48,

E-PAGE™ 96, E-Gel® 48, and E-Gel® 96 gels from Life

Technologies.

Types of

E-Base™

Devices

Mother E-Base™ device (Cat. no. EB-M03)

Built with an electrical plug that can connect directly to

an electrical outlet. Used for electrophoresis of one

E-PAGE™ 48/96 or E-Gel® 48/96 gel.

Daughter E-Base™ device (Cat. no. EB-D03)

Connects to the Mother E-Base™ device, allowing the

independent electrophoresis of two or more E-PAGE™

48/96 or E-Gel® 48/96 gels.

Note: The Daughter E-Base™ device does not have an

electrical plug and cannot be used without a Mother

E-Base

™

device. See pages 5–6 for diagrams of the bases.

E-Base™

Specifications

Dimensions: 14.6 cm × 15 cm × 5.3 cm

Weight: 370 g (Mother E-Base™ device)

271 g (Daughter E-Base™ device)

Safety: Double Insulation

Temperature: Ambient 5°C to 40°C

Built-in Features: Digital time display (00–99 min)

alarm, light LED

• The SBS (Society for Biomolecular Screening)

standard 96-well plate format of the E-Base™ device

fits on most robotic platforms allowing the loading

and electrophoresis of gels on the E-Base™ device

directly on the automated liquid handling system.

• The gel cassette used with the unit shall have

suitable insulation to operate at hazardous voltages

up to 120VDC.

Overview, Continued

Mother

E-Base™

device

Each Mother E-Base™ device has a pwr/prg

(power/program) button (right side) and a time button

(left side) on the lower right side of the base. The lower

left side of each Mother E-Base™ device contains a light

LED and a digital time display (00–99). The gel cassette

is inserted into the two electrode connections. The

Mother E-Base™ device is connected to an electrical

outlet with the electrical plug.

Mother E-Base™ Device

Note: The Mother E-Base™ device has been tested with

up to three Daughter E-Base™ devices connected at one

time.

The E-Base™ device is pre-programmed with 2

programs specific for each gel type as described below:

Program Gel Type

EG E-Gel®

48/96

EP E-PAGE™

48/96

electrode (-)

pwr/prg

button

time button

light LED

digital display

to electrical outlet

electrode (+)

Overview, Continued

Daughter

E-Base™

device

The Daughter E-Base™ device is similar to the Mother

E-Base™ device except the Daughter E-Base™ device

does not have an electrical cord and cannot be

connected to an electrical outlet.

The Daughter E-Base™ device connects to a Mother

E-Base™ device or to another Daughter E-Base™ device

(if already connected to a Mother E-Base™ device). Once

connected to a Mother E-Base™ device, each Daughter

E-Base™ device is designed to function independently of

the Mother E-Base™ device or other Daughter E-Base™

devices.

Mother E-Base™ Device/Daughter E-Base™ Device

Mother

-Base™

Daughter

-Base™

Instructions for Use

Introduction

Instructions to perform electrophoresis using a Mother

E-Base™ device and Daughter E-Base™ device are

described in this section.

For sample preparation, refer to the manual supplied

with the gels.

Connecting

a Daughter

E-Base™

device

Ensure the Mother E-Base™ device is unplugged prior

to attaching a Daughter E-Base™ device.

1. Connect the Daughter E-Base™ device to a Mother

E-Base™ device or another Daughter E-Base™ device

already connected to a Mother E-Base™ device.

2. Plug the Mother E-Base™ device into an electrical

outlet using the electrical plug on the base.

The displays show EP or the last program used (EP

or EG) if there are no gel cassettes on the bases.

Selecting a

Program

Select the appropriate program for your application

prior to inserting a gel into the E-Base™ device.

1. Plug the Mother E-Base™ device into an electrical

outlet using the electrical plug on the base.

The display shows EP or the last program used (EP

or EG) if there is no gel cassette on the base.

2. Select the appropriate program for the type of gel

by pressing and releasing the pwr/prg button:

Gel Type Program Default Run Time

E-Gel® EG 12 minutes

E-PAGE™ EP 14 minutes

Instructions For Use, Continued

Setting the

Run Time

The default time setting for program EG is 12 minutes,

while EP is 14 minutes. Follow the instructions

provided to increase or decrease the time setting

according to the gel type being used.

Gel Type

Recommended

run time

Maximum

run time

E-Gel®

Agarose Gels 20 minutes 25 minutes

E-Gel®

Agarose Gels 12 minutes (EG)

17 minutes

E-PAGE™

8% Gels 25 minutes 30 minutes

E-PAGE™

6% Gels 14 minutes (EP)

25 minutes

Note: Do not exceed the maximum run time listed for

the type of gel when setting the time.

Setting the Time without a Cassette on the Base

To increase or decrease the default run time when no

cassette is on the base, use the following steps:

1. Press and release the time button located on the

lower right corner of the base to view the time

setting.

2. Press and hold the time button to increase the time.

3. When the appropriate run time for the gel type is

reached, release the time button.

If the time button is not released, the time setting

will continue advancing until it reaches 00. To

begin cycling through the numbers again, press the

time button again.

Setting the Time with a Cassette on the Base

To increase the run time when a cassette is inserted

(e.g., during electrophoresis), press and release the time

button to increase the time setting by 1-minute intervals

or press and hold the time button to increase the time

continuously.

To interrupt or stop a run in progress, see page 11.

Instructions for Use, Continued

Running the

Gel

1. Open the package and remove the gel.

2. Remove the plastic comb from the gel.

3. Slide the gel into the Mother E-Base™ device or

Daughter E-Base™ device such that the two copper

electrodes on the right side of the gel cassette come

in contact with the two electrode connections on the

base.

When the gel is properly inserted into the base, the

fan in the base turns on and a red light illuminates

at the lower left corner of the base. The digital

display shows the default time for a selected

program or the last manual setting (Ready Mode).

4. Load the appropriate amount of DNA or protein

samples into sample wells. Load water or sample

buffer containing the same salt concentration as the

sample into any remaining empty wells.

5. Load DNA or protein markers in marker wells.

6. To begin electrophoresis, press and release the

pwr/prg button located on the lower right corner of

the Mother E-Base™ device or Daughter E-Base™

device.

The red light changes to green and the display

counts down the time remaining in the run.

To increase the run time during electrophoresis,

press the time button until the desired time is

reached (see page 8).

To interrupt or stop a run in progress, see page 11.

Electrode

Connections

Instructions for Use, Continued

Running the

Gel,

continued

7. The Mother E-Base™ device or Daughter E-Base™

device signals the end of the run with a flashing red

light and rapid beeping for 2 minutes, followed by a

single beep every minute.

At the end of the run, the display shows the original

time setting (time added during electrophoresis is

not shown). The display also shows the elapsed time

since the end of the run (up to 19 minutes with a

negative sign).

8. Press and release the pwr/prg button to stop the

beeping. The red light becomes steady and the

display shows the last time setting.

9. Remove the gel cassette from the Mother E-Base™

device or Daughter E-Base™ device. Proceed to stain

or capture an image of the gel.

Note: The bands in the gel will diffuse within

20–40 minutes.

We recommend that you disconnect the Mother E-Base™

device from the electrical outlet when not in use for

prolonged periods of time.

Instructions for Use, Continued

Interrupting

a Run

You can interrupt an electrophoresis run at any time by

pressing and releasing the pwr/prg button. The stopped

current is indicated by a steady red light and the digital

display flashes to indicate that the run was interrupted.

You can remove the gel from the E-Base™ device to

check the progress of the run. Then:

• To continue the run from the point at which it was

stopped, reinsert the gel and press and release the

pwr/prg button. The light changes to steady green

and the digital display shows the countdown time.

• To cancel the rest of the interrupted run, press and

hold the pwr/prg button for a few seconds. The

digital display resets and the base returns to Ready

Mode. You can also program a new run time as

described on page 8 and rerun the gel.

In case of an external power failure (loss of electricity or

the electrical cord is accidentally unplugged), the run

will continue when the power resumes. The Mother

E-Base™ device or Daughter E-Base™ device signals the

end of the run as described on page 10, except the light

will be an alternating red/green to indicate that an

external power failure has occurred during the run.

Maintaining

E-Base™

Devices

Keep the surfaces of the Mother E-Base™ and

Daughter E-Base™ devices free of contaminants. To

clean, disconnect bases from power source and wipe

with a dry cloth. Do not attempt to open or service the

bases. To honor the warranty, bases should only be

opened and serviced by Life Technologies.

E-Base™ Quick Reference Guide

Introduction

A quick reference guide for operating the Mother

E-Base™ and Daughter E-Base™ devices is provided in

the following table.

Digital Display Light Sound Action Mode

Without gel

cassette -EP, last

program used

(EP or EG)

With gel cassette

in -last time

setting

No light if a

cassette is

not inserted

or red light

if a cassette

is inserted.

1 beep Mother E-Base™

device connected

to an electrical

outlet

Base

plugged in

Default time

setting

(12 minutes for

EG, 14 minutes

for EP, or last

time setting)

Steady red. — Gel cassette

inserted into a

base

Ready (with

no current

flowing

through gel)

Countdown

time

Steady green. — Press and release

the pwr/prg

button

Run

Negative time

display (00 to

–19 minutes)

Flashing red

until the

time button

is pressed.

Continuous

beeping for

2 minutes

followed by

a single

beep every

minute

Automatic

End of run

Negative time

display (00 to

–19 minutes)

Alternating

red and

green.

Continuous

beeping for

2 minutes

followed by

a single

beep every

minute

Automatic Run ends

after an

external

power

failure

during the

run

Flashing time

display

With gel

cassette in -

steady red

Without gel

cassette - no

light.

— Press and release

the pwr/prg

button during

the run

Pause

(manually

end the run)

E-Base™ Quick Reference Guide, Continued

Digital Display Light Sound Action Mode

Last time

setting.

Steady red

— Press and release

the pwr/prg

button.

Return to

Ready mode

after an

automatic

stop

Countdown

time.

Steady green — Press and release

the pwr/prg

button.

Restart after

a manual

stop

With gel

cassette in –last

time setting

Without gel

cassette - last

program

setting.

With gel

cassette in –

steady red

Without gel

cassette – no

light

— Press and hold

the pwr/prg

button.

Return to

Ready mode

after a

manual stop

Flashing “ER”.

Continuous

loud

beeping

Press and hold

pwr/prg button

for 2 seconds and

remove gel from

the base.

Failure

EP, last program

used (EP or EG).

— — — No cassette

Time increases

by 1 minute

increments.

With gel

cassette –

steady red

— With gel cassette

in - Press and

release the time

button.

Run time

setting

Time increases

continuously

and

automatically

stops at 00.

With gel

cassette in –

steady red

Without gel

cassette – no

light

— With and

without gel

cassette - Press

and hold the

time button.

Selected

program EP or

EG.

No light 1 beep Press and release

the pwr/prg

button when no

cassette is

inserted into the

E-Base™ device to

select the desired

program.

Program

setting

Troubleshooting

Introduction

The following table provides some solutions to the

problems you might encounter during electrophoresis

using E-Base™ devices.

For troubleshooting resolution and sample

preparation problems, refer to the manual supplied

with the gels.

Observation Reason Solution

No current Daughter E-Base™

device used without a

Mother E-Base™

device.

Do not use the Daughter E-Base™

device without a Mother E-Base™

device. The Daughter E-Base™

device does not have an electrical

plug to connect to an electrical

outlet.

Copper contacts in the

Mother E-Base™

device or Daughter

E-Base™ device are

damaged due to

improper use.

Ensure that the copper contact in

the base is intact.

Expired or defective

gel cassette used.

Use fresh gel cassette. Use

properly stored gels before the

specified expiration date.

Gel cassette is not

correctly inserted into

the base.

Remove cassette and reinsert; a

steady red light illuminates

on the

base when the cassette is

correctly

inserted and power is on.

Troubleshooting, Continued

Observation Reason Solution

Over-run the

gel or need

more time to

run gel

Incorrect program

selected

Select EG if you are using

E-Gel® 48 or E-Gel® 96 gels and

EP if you are using E-PAGE™ 48

or E-PAGE™ 96 gels.

If you are at the beginning of the

run, stop the run and select the

desired program.

If you are well into the run, check

the gel to see where the loading

dye is running. Estimate the

amount of time remaining and

then manually stop the run.

Failure

Mode

indicated by

flashing

“ER”, and

continuous

loud beeping

Defective cassette Disconnect E-Base™ device and

remove the gel cassette from the

base.

Press and hold the pwr/prg

button for 2 seconds to return to

Ready Mode. Use a fresh gel

cassette.

Cold cassette Use a room temperature cassette.

Avoid storing gel cassettes at 4°C.

Improper operating

conditions

Use E-Base™ devices at room

temperature (20°C to 25°C).

Appendix

Explanation of Symbols and Warnings

Conformity mark Description

The CE mark symbolizes that the product conforms to all

applicable European Community provisions for which this

marking is required.

The E-Base complies with the TUV Rhineland North

America Inc. safety requirements. The indicators "C" and

"US" mean that the product is certified for both the U.S. and

Canadian markets, to the applicable U.S. and Canadian

standards.

Caution Caution risk of danger. Consult the manual for further

safety information.

Double Insulation

Class II product

Do not dispose of this product in unsorted municipal

waste.

CAUTION! To minimize negative environmental impact

from disposal of electronic waste, do not dispose of

electronic waste in unsorted municipal waste. Follow local

municipal waste ordinances for proper disposal provision

and contact customer service for information about

responsible disposal options.

Saftey

Operation of the E-Base™ device is subject to the following

conditions:

• Do not place the product is a location where the

product’s ventilation can be obstructed.

• Do not place the product on heat-generating surface or

near heat emitting devices such heaters. Verify that

there is sufficient clearance between the product and

any other system that may exhaust warm air.

• The product is for indoor use only

• The product operating ambient range is temperature of

5–40°C (50–95 °F) and Relative humidity of 20–80%.

Life Technologies recommends that an ambient

temperature of 20–25 °C (68–77 °F) and relative

humidity of 30–50% is maintained during normal

operation as this will result in better performance and

longer MTBF of the equipment. Temperature must not

exceed the maximum temperature specified above.

• Maximum operating altitude 2000 m asl, Maximum

transport altitude 4500 m asl.

• Installation categories (over voltage categories) II;

Pollution degree 2

• Mains supply voltage fluctuations not to exceed 10% of

the nominal voltage (100–240 V, 50/60 Hz, 1.5 A).

• Do not connect more than 3 daughter units to a mother

unit

• Mains plug is a disconnect device and must be easily

accessible.

• Do not attempt to open the Mother E-Base™ device or

Daughter E-Base™ device.

• To honor the warranty, E-Base™ devices can only be

opened and serviced by Life Technologies.

• The protection provided by the equipment may be

impaired if the equipment is used in a manner not

specified by Life Technologies.

Explication des symboles et des

avertissements

Marque de

conformité

Description

Le marquage CE symbolise que le produit est conforme à

toutes les dispositions applicables de la Communauté

européenne pour lesquels ce marquage est requis.

L’E-Base est conforme aux exigences de sécurité de TÜV

Rheinland North America Inc. Les indicateurs « C » et

« US » signifient que le produit est certifié pour les marchés

aux États-Unis et au Canada selon les normes américaines

et canadiennes.

Attention

Attention, risque de danger. Consulter le manuel pour

d’autres renseignements de sécurité.

Double isolation

Produit de classe II

Ne pas éliminer ce produit avec les déchets usuels non

soumis au tri sélectif.

ATTENTION! Pour minimiser les conséquences négatives

sur l’environnement à la suite de l’élimination de déchets

électroniques, ne pas éliminer ce déchet électronique avec

les déchets usuels non soumis au tri sélectif. Se conformer

aux ordonnances locales sur les déchets municipaux pour

les dispositions d’élimination et communiquer avec le

service à la clientèle pour des renseignements sur les

options d’élimination responsable.

Sécurité

L’utilisation du dispositif E

BaseMC est soumise aux

conditions suivantes :

• Ne pas placer le produit dans un endroit où sa

ventilation risque d’être obstruée.

• Ne pas placer le produit sur une surface générant de la

chaleur ou près de dispositifs émettant de la chaleur,

tels que des appareils de chauffage. Vérifier qu’il y a

suffisamment d’espace entre le produit et tout autre

système pouvant évacuer de l’air chaud.

• Le produit est destiné à une utilisation en intérieur

uniquement.

• La plage d’utilisation normale du produit est de 5 à

40 °C (50 à 95 °F) en matière de température et de 20 à

80 % en matière d’humidité relative. Life Technologies

conseille aux utilisateurs de conserver une température

ambiante de 20 à 25 °C (68 à 77 °F) et une humidité

relative de 30 à 50 % au cours du fonctionnement

normal de l’équipement de façon à en optimiser les

performances et à accroître le temps moyen entre

défaillances. La température ne doit pas dépasser la

limite supérieure de la plage spécifiée ci-dessus.

• Altitude maximale d’utilisation de 2 000 m au-dessus

du niveau de la mer, altitude maximale de transport de

4 500 m au-dessus du niveau de la mer.

• Catégories d’installation (catégories de surtension) II;

degré de pollution 2.

• Les fluctuations de tension de l’alimentation secteur ne

doivent pas dépasser 10 % de la tension nominale (100

à 240 V, 50/60 Hz, 1,5 A).

• Ne pas brancher plus de 3 unités dépendantes à la

même unité principale.

• La fiche secteur est un dispositif de déconnexion et

doit être facilement accessible.

• Ne pas tenter d’ouvrir le dispositif E-BaseMC principal

ou le dispositif E-BaseMC dépendant.

• Pour honorer la garantie, les dispositifs E-BaseMC ne

peuvent être ouverts et entretenus que par

Life Technologies.

• La protection fournie par l’équipement risque d’être

altérée si l’équipement est utilisé d’une manière non

spécifiée par Life Technologies.

Accessory Products

Additional

Products

Additional products available separately from Life

Technologies are listed in the following table. For

more information on these products, go to

www.lifetechnologies.com or contact Technical

Support, page 21.

Product Quantity Catalog No.

E-PAGE™ 48 8% Gels 8 gels EP048-08

E-PAGE

™

96 6% Gels 8 gels EP096-06

1% E-Gel® 96 Agarose 8 gels G7008-01

2% E-Gel® 96 Agarose 8 gels G7008-02

E-Gel® 48 1% Agarose Gels 8 gels G8008-01

E-Gel® 48 2% Agarose Gels 8 gels G8008-02

E-Gel

48 4% Agarose Gels 8 gels G8008-04

E-Holder™ Platform 2 each EH-03

Molecular

Weight

Markers

A variety of DNA and protein molecular weight

markers are available from Life Technologies. The

recommended marker for each gel type is listed in

the manual supplied with the gels. For more

information, visit www.lifetechnologies.com or

contact Technical Support, page 21.

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Model: E-Base Electrophoresis

Type: User guide

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