Thermo Fisher Scientific E-Base Electrophoresis User guide

Type
User guide
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
E-Base Electrophoresis Device
Catalog Numbers EB-M03 and EB-D03
Publication Part Number 25-0667
Publication Number MAN0002597
Revision A
For Research Use Only. Not for use in diagnostic procedures.
Information in this document is subject to change without notice.
DISCLAIMER
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL
WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING
BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR
PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT
SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN
CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES
IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT
LIMITED TO THE USE THEREOF.
IMPORTANT LICENSING INFORMATION
These products may be covered by one or more Limited Use Label Licenses. By use of
these products, you accept the terms and conditions of all applicable Limited Use Label
Licenses.
TRADEMARKS
© 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of
Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
2
Contents
Overview........................................................................................................ 4
Instructions for Use ...................................................................................... 7
E-Base Quick Reference Guide ............................................................... 12
Troubleshooting .......................................................................................... 14
Appendix............................................................................ 16
Explanation of Symbols and Warnings ................................................... 16
Saftey ............................................................................................................ 17
Explication des symboles et des avertissements .................................... 18
Sécurité ......................................................................................................... 19
Accessory Products .................................................................................... 20
Technical Support ....................................................................................... 21
3
Overview
Introduction
The E-Base device is an easy-to-use, programmable,
automated device combining a base and power supply
to simplify electrophoresis of pre-cast E-PAGE 48,
E-PAGE 96, E-Gel® 48, and E-Gel® 96 gels from Life
Technologies.
Types of
E-Base
Devices
Mother E-Base device (Cat. no. EB-M03)
Built with an electrical plug that can connect directly to
an electrical outlet. Used for electrophoresis of one
E-PAGE 48/96 or E-Gel® 48/96 gel.
Daughter E-Base device (Cat. no. EB-D03)
Connects to the Mother E-Base device, allowing the
independent electrophoresis of two or more E-PAGE
48/96 or E-Gel® 48/96 gels.
Note: The Daughter E-Base device does not have an
electrical plug and cannot be used without a Mother
E-Base
device. See pages 5–6 for diagrams of the bases.
E-Base
Specifications
Dimensions: 14.6 cm × 15 cm × 5.3 cm
Weight: 370 g (Mother E-Base device)
271 g (Daughter E-Base device)
Safety: Double Insulation
Temperature: Ambient 5°C to 40°C
Built-in Features: Digital time display (0099 min)
alarm, light LED
The SBS (Society for Biomolecular Screening)
standard 96-well plate format of the E-Base device
fits on most robotic platforms allowing the loading
and electrophoresis of gels on the E-Base device
directly on the automated liquid handling system.
The gel cassette used with the unit shall have
suitable insulation to operate at hazardous voltages
up to 120VDC.
4
Overview, Continued
Mother
E-Base
device
Each Mother E-Base device has a pwr/prg
(power/program) button (right side) and a time button
(left side) on the lower right side of the base. The lower
left side of each Mother E-Base device contains a light
LED and a digital time display (0099). The gel cassette
is inserted into the two electrode connections. The
Mother E-Base device is connected to an electrical
outlet with the electrical plug.
Mother E-Base Device
Note: The Mother E-Base device has been tested with
up to three Daughter E-Base devices connected at one
time.
The E-Base device is pre-programmed with 2
programs specific for each gel type as described below:
Program Gel Type
EG E-Gel®
48/96
EP E-PAGE
48/96
electrode (-)
pwr/prg
button
time button
digital display
to electrical outlet
electrode (+)
5
Overview, Continued
Daughter
E-Base
device
The Daughter E-Base device is similar to the Mother
E-Base device except the Daughter E-Base device
does not have an electrical cord and cannot be
connected to an electrical outlet.
The Daughter E-Base device connects to a Mother
E-Base device or to another Daughter E-Base device
(if already connected to a Mother E-Base device). Once
connected to a Mother E-Base device, each Daughter
E-Base device is designed to function independently of
the Mother E-Base device or other Daughter E-Base
devices.
Mother E-BaseDevice/Daughter E-BaseDevice
Mother
E
-Base
Daughter
E
-Base
6
Instructions for Use
Introduction
Instructions to perform electrophoresis using a Mother
E-Base device and Daughter E-Base device are
described in this section.
For sample preparation, refer to the manual supplied
with the gels.
Connecting
a Daughter
E-Base
device
Ensure the Mother E-Base device is unplugged prior
to attaching a Daughter E-Base device.
1. Connect the Daughter E-Base device to a Mother
E-Base device or another Daughter E-Base device
already connected to a Mother E-Base device.
2. Plug the Mother E-Base device into an electrical
outlet using the electrical plug on the base.
The displays show EP or the last program used (EP
or EG) if there are no gel cassettes on the bases.
Selecting a
Program
Select the appropriate program for your application
prior to inserting a gel into the E-Base device.
1. Plug the Mother E-Base device into an electrical
outlet using the electrical plug on the base.
The display shows EP or the last program used (EP
or EG) if there is no gel cassette on the base.
2. Select the appropriate program for the type of gel
by pressing and releasing the pwr/prg button:
Gel Type Program Default Run Time
E-Gel® EG 12 minutes
E-PAGE EP 14 minutes
7
Instructions For Use, Continued
Setting the
Run Time
The default time setting for program EG is 12 minutes,
while EP is 14 minutes. Follow the instructions
provided to increase or decrease the time setting
according to the gel type being used.
Gel Type
Recommended
run time
Maximum
run time
E-Gel®
48
Agarose Gels 20 minutes 25 minutes
E-Gel®
96
Agarose Gels 12 minutes (EG)
17 minutes
E-PAGE
48
8% Gels 25 minutes 30 minutes
E-PAGE
96
6% Gels 14 minutes (EP)
25 minutes
Note: Do not exceed the maximum run time listed for
the type of gel when setting the time.
Setting the Time without a Cassette on the Base
To increase or decrease the default run time when no
cassette is on the base, use the following steps:
1. Press and release the time button located on the
lower right corner of the base to view the time
setting.
2. Press and hold the time button to increase the time.
3. When the appropriate run time for the gel type is
reached, release the time button.
If the time button is not released, the time setting
will continue advancing until it reaches 00. To
begin cycling through the numbers again, press the
time button again.
Setting the Time with a Cassette on the Base
To increase the run time when a cassette is inserted
(e.g., during electrophoresis), press and release the time
button to increase the time setting by 1-minute intervals
or press and hold the time button to increase the time
continuously.
To interrupt or stop a run in progress, see page 11.
8
Instructions for Use, Continued
Running the
Gel
1. Open the package and remove the gel.
2. Remove the plastic comb from the gel.
3. Slide the gel into the Mother E-Base device or
Daughter E-Base device such that the two copper
electrodes on the right side of the gel cassette come
in contact with the two electrode connections on the
base.
When the gel is properly inserted into the base, the
fan in the base turns on and a red light illuminates
at the lower left corner of the base. The digital
display shows the default time for a selected
program or the last manual setting (Ready Mode).
4. Load the appropriate amount of DNA or protein
samples into sample wells. Load water or sample
buffer containing the same salt concentration as the
sample into any remaining empty wells.
5. Load DNA or protein markers in marker wells.
6. To begin electrophoresis, press and release the
pwr/prg button located on the lower right corner of
the Mother E-Base device or Daughter E-Base
device.
The red light changes to green and the display
counts down the time remaining in the run.
To increase the run time during electrophoresis,
press the time button until the desired time is
reached (see page 8).
To interrupt or stop a run in progress, see page 11.
Electrode
Connections
9
Instructions for Use, Continued
Running the
Gel,
continued
7. The Mother E-Base device or Daughter E-Base
device signals the end of the run with a flashing red
light and rapid beeping for 2 minutes, followed by a
single beep every minute.
At the end of the run, the display shows the original
time setting (time added during electrophoresis is
not shown). The display also shows the elapsed time
since the end of the run (up to 19 minutes with a
negative sign).
8. Press and release the pwr/prg button to stop the
beeping. The red light becomes steady and the
display shows the last time setting.
9. Remove the gel cassette from the Mother E-Base
device or Daughter E-Base device. Proceed to stain
or capture an image of the gel.
Note: The bands in the gel will diffuse within
2040 minutes.
We recommend that you disconnect the Mother E-Base
device from the electrical outlet when not in use for
prolonged periods of time.
10
Instructions for Use, Continued
Interrupting
a Run
You can interrupt an electrophoresis run at any time by
pressing and releasing the pwr/prg button. The stopped
current is indicated by a steady red light and the digital
display flashes to indicate that the run was interrupted.
You can remove the gel from the E-Base device to
check the progress of the run. Then:
To continue the run from the point at which it was
stopped, reinsert the gel and press and release the
pwr/prg button. The light changes to steady green
and the digital display shows the countdown time.
To cancel the rest of the interrupted run, press and
hold the pwr/prg button for a few seconds. The
digital display resets and the base returns to Ready
Mode. You can also program a new run time as
described on page 8 and rerun the gel.
In case of an external power failure (loss of electricity or
the electrical cord is accidentally unplugged), the run
will continue when the power resumes. The Mother
E-Base device or Daughter E-Base device signals the
end of the run as described on page 10, except the light
will be an alternating red/green to indicate that an
external power failure has occurred during the run.
Maintaining
E-Base
Devices
Keep the surfaces of the Mother E-Base and
Daughter E-Base devices free of contaminants. To
clean, disconnect bases from power source and wipe
with a dry cloth. Do not attempt to open or service the
bases. To honor the warranty, bases should only be
opened and serviced by Life Technologies.
11
E-Base Quick Reference Guide
Introduction
A quick reference guide for operating the Mother
E-Base and Daughter E-Base devices is provided in
the following table.
Digital Display Light Sound Action Mode
Without gel
cassette -EP, last
program used
(EP or EG)
With gel cassette
in -last time
setting
No light if a
cassette is
not inserted
or red light
if a cassette
is inserted.
1 beep Mother E-Base
device connected
to an electrical
outlet
Base
plugged in
Default time
setting
(12 minutes for
EG, 14 minutes
for EP, or last
time setting)
Steady red. Gel cassette
inserted into a
base
Ready (with
no current
flowing
through gel)
Countdown
time
Steady green. Press and release
the pwr/prg
button
Run
Negative time
display (00 to
19 minutes)
Flashing red
until the
time button
is pressed.
Continuous
beeping for
2 minutes
followed by
a single
beep every
minute
Automatic
End of run
Negative time
display (00 to
19 minutes)
Alternating
red and
green.
Continuous
beeping for
2 minutes
followed by
a single
beep every
minute
Automatic Run ends
after an
external
power
failure
during the
run
Flashing time
display
With gel
cassette in -
steady red
Without gel
cassette - no
light.
Press and release
the pwr/prg
button during
the run
Pause
(manually
end the run)
12
E-Base Quick Reference Guide, Continued
Digital Display Light Sound Action Mode
Last time
setting.
Steady red
Press and release
the pwr/prg
button.
Return to
Ready mode
after an
automatic
stop
Countdown
time.
Steady green Press and release
the pwr/prg
button.
Restart after
a manual
stop
With gel
cassette in last
time setting
Without gel
cassette - last
program
setting.
With gel
cassette in
steady red
Without gel
cassette no
light
Press and hold
the pwr/prg
button.
Return to
Ready mode
after a
manual stop
Flashing “ER”.
Continuous
loud
beeping
Press and hold
pwr/prg button
for 2 seconds and
remove gel from
the base.
Failure
EP, last program
used (EP or EG).
No cassette
Time increases
by 1 minute
increments.
With gel
cassette
steady red
With gel cassette
in - Press and
release the time
button.
Run time
setting
Time increases
continuously
and
automatically
stops at 00.
With gel
cassette in
steady red
Without gel
cassette no
light
With and
without gel
cassette - Press
and hold the
time button.
Selected
program EP or
EG.
No light 1 beep Press and release
the pwr/prg
button when no
cassette is
inserted into the
E-Base device to
select the desired
program.
Program
setting
13
Troubleshooting
Introduction
The following table provides some solutions to the
problems you might encounter during electrophoresis
using E-Base devices.
For troubleshooting resolution and sample
preparation problems, refer to the manual supplied
with the gels.
Observation Reason Solution
No current Daughter E-Base
device used without a
Mother E-Base
device.
Do not use the Daughter E-Base
device without a Mother E-Base
device. The Daughter E-Base
device does not have an electrical
plug to connect to an electrical
outlet.
Copper contacts in the
Mother E-Base
device or Daughter
E-Base device are
damaged due to
improper use.
Ensure that the copper contact in
the base is intact.
Expired or defective
gel cassette used.
Use fresh gel cassette. Use
properly stored gels before the
specified expiration date.
Gel cassette is not
correctly inserted into
the base.
Remove cassette and reinsert; a
steady red light illuminates
on the
base when the cassette is
correctly
inserted and power is on.
14
Troubleshooting, Continued
Observation Reason Solution
Over-run the
gel or need
more time to
run gel
Incorrect program
selected
Select EG if you are using
E-Gel® 48 or E-Gel® 96 gels and
EP if you are using E-PAGE 48
or E-PAGE 96 gels.
If you are at the beginning of the
run, stop the run and select the
desired program.
If you are well into the run, check
the gel to see where the loading
dye is running. Estimate the
amount of time remaining and
then manually stop the run.
Failure
Mode
indicated by
flashing
“ER”, and
continuous
loud beeping
Defective cassette Disconnect E-Base device and
remove the gel cassette from the
base.
Press and hold the pwr/prg
button for 2 seconds to return to
Ready Mode. Use a fresh gel
cassette.
Cold cassette Use a room temperature cassette.
Avoid storing gel cassettes at 4°C.
Improper operating
conditions
Use E-Base devices at room
temperature (20°C to 25°C).
15
Appendix
Explanation of Symbols and Warnings
Conformity mark Description
The CE mark symbolizes that the product conforms to all
applicable European Community provisions for which this
marking is required.
The E-Base complies with the TUV Rhineland North
America Inc. safety requirements. The indicators "C" and
"US" mean that the product is certified for both the U.S. and
Canadian markets, to the applicable U.S. and Canadian
standards.
Caution Caution risk of danger. Consult the manual for further
safety information.
Double Insulation
Class II product
Do not dispose of this product in unsorted municipal
waste.
CAUTION! To minimize negative environmental impact
from disposal of electronic waste, do not dispose of
electronic waste in unsorted municipal waste. Follow local
municipal waste ordinances for proper disposal provision
and contact customer service for information about
responsible disposal options.
16
Saftey
Operation of the E-Base device is subject to the following
conditions:
Do not place the product is a location where the
product’s ventilation can be obstructed.
Do not place the product on heat-generating surface or
near heat emitting devices such heaters. Verify that
there is sufficient clearance between the product and
any other system that may exhaust warm air.
The product is for indoor use only
The product operating ambient range is temperature of
5–40°C (5095 °F) and Relative humidity of 2080%.
Life Technologies recommends that an ambient
temperature of 2025 °C (68–77 °F) and relative
humidity of 3050% is maintained during normal
operation as this will result in better performance and
longer MTBF of the equipment. Temperature must not
exceed the maximum temperature specified above.
Maximum operating altitude 2000 m asl, Maximum
transport altitude 4500 m asl.
Installation categories (over voltage categories) II;
Pollution degree 2
Mains supply voltage fluctuations not to exceed 10% of
the nominal voltage (100240 V, 50/60 Hz, 1.5 A).
Do not connect more than 3 daughter units to a mother
unit
Mains plug is a disconnect device and must be easily
accessible.
Do not attempt to open the Mother E-Base device or
Daughter E-Base device.
To honor the warranty, E-Base devices can only be
opened and serviced by Life Technologies.
The protection provided by the equipment may be
impaired if the equipment is used in a manner not
specified by Life Technologies.
17
Explication des symboles et des
avertissements
Marque de
conformi
Description
Le marquage CE symbolise que le produit est conforme à
toutes les dispositions applicables de la Communauté
européenne pour lesquels ce marquage est requis.
L’E-Base est conforme aux exigences de sécurité de TÜV
Rheinland North America Inc. Les indicateurs « C » et
« US » signifient que le produit est certifié pour les marchés
aux États-Unis et au Canada selon les normes américaines
et canadiennes.
Attention
Attention, risque de danger. Consulter le manuel pour
d’autres renseignements de sécurité.
Double isolation
Produit de classe II
Ne pas éliminer ce produit avec les déchets usuels non
soumis au tri sélectif.
ATTENTION! Pour minimiser les conséquences négatives
sur l’environnement à la suite de l’élimination de déchets
électroniques, ne pas éliminer ce déchet électronique avec
les déchets usuels non soumis au tri sélectif. Se conformer
aux ordonnances locales sur les déchets municipaux pour
les dispositions d’élimination et communiquer avec le
service à la clientèle pour des renseignements sur les
options d’élimination responsable.
18
Sécurité
L’utilisation du dispositif E
-
BaseMC est soumise aux
conditions suivantes :
Ne pas placer le produit dans un endroit où sa
ventilation risque d’être obstruée.
Ne pas placer le produit sur une surface générant de la
chaleur ou près de dispositifs émettant de la chaleur,
tels que des appareils de chauffage. Vérifier qu’il y a
suffisamment d’espace entre le produit et tout autre
système pouvant évacuer de l’air chaud.
Le produit est destiné à une utilisation en intérieur
uniquement.
La plage d’utilisation normale du produit est de 5 à
40 °C (50 à 95 °F) en matière de température et de 20 à
80 % en matière d’humidité relative. Life Technologies
conseille aux utilisateurs de conserver une température
ambiante de 20 à 25 °C (68 à 77 °F) et une humidité
relative de 30 à 50 % au cours du fonctionnement
normal de l’équipement de façon à en optimiser les
performances et à accroître le temps moyen entre
défaillances. La température ne doit pas dépasser la
limite supérieure de la plage spécifiée ci-dessus.
Altitude maximale d’utilisation de 2 000 m au-dessus
du niveau de la mer, altitude maximale de transport de
4 500 m au-dessus du niveau de la mer.
Catégories d’installation (catégories de surtension) II;
degré de pollution 2.
Les fluctuations de tension de l’alimentation secteur ne
doivent pas dépasser 10 % de la tension nominale (100
à 240 V, 50/60 Hz, 1,5 A).
Ne pas brancher plus de 3 unités dépendantes à la
même unité principale.
La fiche secteur est un dispositif de déconnexion et
doit être facilement accessible.
Ne pas tenter d’ouvrir le dispositif E-BaseMC principal
ou le dispositif E-BaseMC dépendant.
Pour honorer la garantie, les dispositifs E-BaseMC ne
peuvent être ouverts et entretenus que par
Life Technologies.
La protection fournie par l’équipement risque d’être
altérée si l’équipement est utilisé d’une manière non
spécifiée par Life Technologies.
19
Accessory Products
Additional
Products
Additional products available separately from Life
Technologies are listed in the following table. For
more information on these products, go to
www.lifetechnologies.com or contact Technical
Support, page 21.
Product Quantity Catalog No.
E-PAGE 48 8% Gels 8 gels EP048-08
E-PAGE
96 6% Gels 8 gels EP096-06
1% E-Gel® 96 Agarose 8 gels G7008-01
2% E-Gel® 96 Agarose 8 gels G7008-02
E-Gel® 48 1% Agarose Gels 8 gels G8008-01
E-Gel® 48 2% Agarose Gels 8 gels G8008-02
E-Gel
®
48 4% Agarose Gels 8 gels G8008-04
E-HolderPlatform 2 each EH-03
Molecular
Weight
Markers
A variety of DNA and protein molecular weight
markers are available from Life Technologies. The
recommended marker for each gel type is listed in
the manual supplied with the gels. For more
information, visit www.lifetechnologies.com or
contact Technical Support, page 21.
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Thermo Fisher Scientific E-Base Electrophoresis User guide

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