1
SmartDoc
™
2.0
E5001-SDB
Instruction Manual
Version 11.16
2
Table of Contents
1. Introduction …………………………………………… 3
2. Warnings ………………………………………………. 3
3. Unpacking …………………………………………….. 4
4. SmartDoc 2.0 Overview ………………………… 4
5. Setting up the SmartDoc 2.0…………………… 5
6. Gel Viewing and Band Cutting ……………….. 6
7. SmartDoc Photo Filters ………..………………… 6
8. Imaging with Smart Phone ……………………… 7
9. Specifications ………………………………………….. 8
10. Accessories and Consumables …………… 8
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1. INTRODUCTION
Congratulations on your purchase of a SmartDoc 2.0 System. This innovative
and easy to use system allows visualization of stained nucleic acids in
electrophoresis gels, and can also be used for capturing high quality photos with a
smart phone camera. The blue light illumination system produces intense blue
light at a wavelength that is ideal for excitation of most of the common, green
stains available of the market, including SYBR™ strains and Accuris™ SmartGlow™
stains. The orange filter cover and orange photo filters included with the system
are designed to absorb (block) the blue excitation light allowing only the
fluorescing sample light to pass through for visualization by eye, or for capturing
gel images using a smart phone or tablet with camera.
To ensure proper operation and performance from your SmartDoc System,
please read this manual in its entirety before use.
2. WARNINGS
Read this manual in its entirety before operating the SmartDoc system, and keep
the manual for future reference.
CAUTION: Although the light emitted from the SmartDoc illumination
base is within the visible spectrum, and does not pose the same
hazards as UV light, this emitted blue light is very bright. It is not
recommended to look directly at the blue light array (just as we recommend not
to stare at the sun.)
CAUTION: When working in a laboratory environment, it is always
important to properly handle all reagents and chemicals. When using
a cell phone for capturing gel images, it is very important not to
contaminate the phone with any chemicals or reagents. Using use proper and
common laboratory safety practices when working with reagents and gels stained
with DNA dye. Always wear gloves, and always change gloves when switching
from handling gels, or reagent to use of a cell phone, and vice versa. The
SmartDoc System components should be cleaned periodically using mild soap and
water or 10% bleach solution for decontamination.
CAUTION: If using the SmartDoc enclosure on a UV transilluminator,
always use caution to prevent exposure of the skin and eyes to UV
light. If working on a UV transilluminator with a viewing surface larger
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than the bottom of the SmartDoc housing, use the E5000-MAT UV Blocking Mat
to block UV light from emitting around the edges of the SmartDoc.
3. UNPACKING
NOTE: Always handle the SmartDoc system with care.
Carefully remove the system and all accessories from the carton and remove any
foam protective pieces. Check for shipping damage. In the event of shipping
damage, a claim must be filed with the carrier. Check the contents of the package
to make sure you have received all of the parts ordered:
SmartDoc Imaging Enclosure parts: 1) Main enclosure hood, 2) extending
adapter, and 3) top platform for smartphone.
Orange Photo Filter
Gel preparation and cutting surface
Illumination base
12 VDC Power Supply with proper cord for local outlet type
Any additional filters for UV (ordered separately)
If any parts are damaged or missing, please immediately contact Benchmark
Scientific Customer Service at 908-769-5555 or email
info@benchmarkscientific.com.
IMPORTANT: Save the carton and packing materials for storing and
transporting the SmartDoc or returning it for any required servicing.
4. SmartDoc 2.0 Assembly, Overview of Parts
Smart phone platform
Removable extender
(remove for close up
imaging)
Orange Photo filter
Main Enclosure Hood
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4. SETTING UP THE SMARTDOC
Assemble the components of the SmartDoc 2.0 System as pictured in section 3 of
the manual. Depending on the size of the gels to be imaged, or the desired detail
of the image, the extender piece can be connected or removed. The top, smart
phone platform piece connects either to the top of the extender or to the top of
the main enclosure. These parts will snap together and pull apart with gentle
pressure.
Once the top platform, extender and main housing parts are assembled, these
can be fit over the illumination base, which in turn fits over the gel preparation
surface.
Locate the SmartDoc near an electrical outlet. Plug the appropriate end of the
power adapter cord into the 12V DC input jack on the illumination base. Plug one
end of the power cord into the adapter box and the other end into a suitable
outlet. Press the power switch to check operation of the lights.
12V DC input jack
Power switch
Gel Preparation and
cutting surface
Blue light
illumination base
AC/DC 12V Power
Pack and Plug Cord
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Note: The SmartDoc uses a timing circuit, and the lights will automatically turn
off after 5 minutes. This timer prevents excess heat from building up from the
powerful LED lights.
5. GEL VIEWING AND BAND CUTTING
The SmartDoc Illumination Base can be used with the orange filter cover for
visualizing stained nucleic acids Gel Viewing separated on agarose or acylamide
gels.
1. Set the gel preparation platform onto a level lab bench or table.
2. Glean the glass surface with alcohol or glass cleaner and allow to dry.
3. Carefully place a stained electrophoresis gel onto the square glass
surface using care to eliminate bubbles under the gel.
4. Note: The maximum sized gel to fit onto the glass surface is 15x15cm,
cut away any overhanging edges of the gel if required.
5. Place the blue illumination base over the gel preparation platform,
taking care that it nests correctly into place.
6. SMARTDOC PHOTO FILTERS
A photo filter is required to capture quality images with the SmartDoc 2.0 system.
The orange photo filter, part E5001-ORANGE, is included with the system. This
filter blocks the visible blue wavelengths coming from the illumination base or
E5001-ORANGE
(included)
E5001-535
(optional)
E5001-590
(optional)
Orange filter cover placed for gel viewing
Orange filter cover set at an angle for gel
access and band cutting
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other blue light transilluminators and allows through the emission wavelengths
from fluorescing nucleic acid stains.
Additional filters are available separately for the SmartDoc and are recommended
for using the SmartDoc enclosure on a UV Transilluminator. These glass band
pass filters block the UV light so the UV bulbs will not be seen in the images, and
they also eliminate background wavelengths from the excitation source.
E5001-590
band pass photo filter, 590nm, for imaging EtBR on UV transilluminator
E5001-535
band pass photo filter, 535nm for imaging green stains on UV transilluminator
Caution: Using the SmartDoc on a UV Transilluminator without an
appropriate filter in place can cause eye or skin exposure to UV
radiation. The SmartDoc filters effectively block harmful UV radiation,
and should always be properly installed when using UV illumination.
7. GEL IMAGING WITH THE SMART DOC
The SmartDoc 2.0 Illumination base produces visible blue light with peak output
of 465nm for excitation of common nucleic acid stains. Compatible stains include
Accuris SmartGlow™ PS (Pre Stain), SmartGlow™ LD (Loading Dye), SYBR® Green I
and II, SYBR® Safe, SYBR® Gold, Gel Star, Gel Green, and Green Glo™.
1. Set the gel preparation platform onto a level lab bench or table.
2. Glean the glass surface with alcohol or glass cleaner and allow to dry.
3. Carefully place a stained electrophoresis gel onto the square glass surface
using care to eliminate bubbles under the gel.
4. Note: The maximum sized gel to fit onto the glass surface is 15x15cm, cut
away any overhanging edges of the gel if required.
5. Place the blue illumination base over the gel preparation platform, taking
care that it nests correctly into place.
6. Place the SmartDoc imaging enclosure over the illumination base.
7. Insert the E5001-ORANGE filter into place on the top platform. The side
with the Accuris logo should be facing up.
8. Place a smartphone face down onto the top platform, and align the camera
lens with the filter.
9. Press the power switch on the illumination base to turn on the blue LEDs.
10. Select the Camera Mode on your Smart Phone, and turn off the flash
setting. When properly positioned, the gel will be seen in the device’s
display screen. Focus as required.
11. If required, remove or insert the extender piece to maximize the image size
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of the gel in the display.
12. A zoom function on the camera phone can be used to enlarge the gel
image in the display, but this can decrease the resolution of the image.
8. SPECIFICATIONS:
9. ACCESSORIES AND CONSUMABLES
Item No.
Description
E5001-ORANGE
Orange photo filter
E5001-590
band pass photo filter, 590nm, for imaging EtBR on UV transilluminator
E5001-535
band pass photo filter, 535nm for imaging green stains on UV
transilluminator
E5001-OC
Orange filter cover
E4500-LD
SmartGlow™ Loading Dye with Safe Green Stain, 1.0ml
E4500-LD-S
SmartGlow™ Loading Dye with Safe Green Stain, Sample, 20ul
E4500-PS
SmartGlow™ Safe Green Pre Stain, 1.0ml
E4500-PS
SmartGlow™ Safe Green Pre Stain, Sample, 40ul
© Copyright, 2016, Benchmark Scientific
PO Box 709
Edison, NJ 08818
USA
Phone: 908-769-5555
Email:
www.benchmarkscientific.com / www.accuris-usa.com
Maximum Gel Size:
15 x 15 cm
Excitation Source:
230 high intensity blue LEDs, peak output 465nm
Orange imaging filter:
Orange PMMA, 12mm aperture
Orange viewing cover:
Orange PMMA, 18 x 18 cm
Dimensions (WxDxH):
23 x 19 x 22 cm
Weight:
0.9 kg
Phone compatibility:
iPhone, Samsung, LG, HTC smartphones and tablets with
camera
Electrical*:
12VDC power supply Included.
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