■CHAPTER5Genexus™ Multisample DNA Purification Kit protocol ........... 66
Plan a purification run (standaloneconfiguration) ................................... 68
Add a purification run plan (standaloneconfiguration) .......................... 68
Prepare the Quantitation Plate andconsumables ................................... 71
Equilibrate the Quantitation Plate ............................................ 72
Prepare theconsumables ................................................... 72
Prepare sample lysates ......................................................... 72
Proceduralguidelines ...................................................... 72
Before each use of thekit ................................................... 73
Materials required .......................................................... 73
Load the Genexus™ Purification Instrument and start therun ........................ 73
Start the purificationrun .................................................... 73
Load the Genexus™ PurificationInstrument ................................... 74
Unload purifiedsamples ........................................................ 82
View and export quantitation results ......................................... 84
Add controls to the sample input plate—Sample to Resultrun ................... 86
Dilute the samples and load the sample input plate—Nucleic Acid to Resultrun ... 87
Dispose used consumables and UV clean theinstrument ....................... 87
■CHAPTER6Load the sequencer and start arun ............................... 89
Before youbegin ............................................................... 89
Load the sequencer and start arun .............................................. 91
Clear the instrument deck and perform a UVClean ................................. 98
■CHAPTER7Review data and results .......................................... 101
Review run results ............................................................ 102
View the run summary .................................................... 104
Assign PCR Plate ......................................................... 108
Review samples for Sample to Resultruns ....................................... 109
Variant report ................................................................. 109
Generate a variant report .................................................. 110
Download a variant report ................................................. 111
View sequencing results ....................................................... 111
Assay metrics and the run report ........................................... 112
Run report ............................................................... 116
QC results ............................................................... 116
View KeyFindings ........................................................ 119
Summary of the SampleResults ............................................ 125
View SNV/INDEL results ................................................... 127
View CNV results ......................................................... 129
Visualize exon and whole geneCNVs ....................................... 132
Search and filter variant results ............................................. 134
Contents
Oncomine™ BRCA Assay GX User Guide 5