Thermo Fisher Scientific GeneMapper® ID-X Software Owner's manual

Brand: Thermo Fisher Scientific

Model: GeneMapper® ID-X Software

Type: Owner's manual

GeneMapper® ID-X Software Version 1.2

USER BULLETIN | DECEMBER 2010

SUBJECT: Verification Experiments and Installation Procedures

Purpose of this

user bulletin

This user bulletin:

• Describes the results of experiments performed by Life Technologies to verify the

following:

– The GeneMapper® ID-X Software Version 1.2 performs as designed to

analyze data generated on legacy human identification capillary

electrophoresis platforms such as the ABI PRISM® 310, 3100, 3100-Avant,

3130, and 3130xl Genetic Analyzers, and the 3730 DNA Analyzer.

– The new features added do not adversely affect the software functionality

carried over from GeneMapper® ID-X Software Version 1.1.1.

• Provides instructions for installing GeneMapper® ID-X Software Version 1.2.

In this user bulletin This user bulletin covers:

■About GeneMapper® ID-X Software Version 1.2. . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

■Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Software verification testing and results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Sizing and genotyping concordance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Mixture analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

■Installing GeneMapper® ID-X Software Version 1.2 . . . . . . . . . . . . . . . . . . . . . . . . . 9

General installation instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

About co-installation with Data Collection Software . . . . . . . . . . . . . . . . . . . . . . . . 9

Perform a fresh GeneMapper® ID-X Software Version 1.2 installation . . . . . . . . . 9

Upgrade from a previous version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Obtaining support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

About GeneMapper® ID-X Software Version 1.2

GeneMapper® ID-X Software Version 1.2 contains the same functionality as

GeneMapper® ID-X Software Version 1.1.1, with additional capability to analyze .hid

sample files generated on the 3500 and 3500xL Genetic Analyzers.

Features incorporated from v1.1.1 software include:

• An option to separate each sample plot and table on individual pages when

printing multiple samples at a time

• An option to enable/disable spike detection for allelic ladder samples

• Enhancements to printing speed to decrease total print time and increase print

speed per page

The full list of features incorporated from version 1.1.1 are described in more detail in

the GeneMapper® ID-X Software Version 1.1.1 User Bulletin (PN 4444446), which is

available to view or download at: http://www3.appliedbiosystems.com/cms/groups/

applied_markets_support/documents/generaldocuments/cms_072003.pdf

New features in version 1.2 software include:

• The ability to analyze .hid sample files generated on the 3500 and 3500xL Genetic

Analyzers and view additional sample file information

• The ability to enable/disable the normalization of sample peaks when the

normalization feature is enabled in 3500 and 3500xL Data Collection Software

• Compatibility with Windows Vista® in addition to Windows® XP and Windows®

2000 Professional Operating Systems

• Updated database to the newest Oracle 11G database

Verification

Software

verification testing

and results

The tests performed in this validation include the following:

• Concordance testing comparing a historical data set collected from legacy Genetic

Analyzer instruments using a variety of AmpFlSTR® kits analyzed using

GeneMapper® ID-X Software versions 1.1 and 1.2 (compare data in Genotypes

and Sizing tables).

• Concordance testing comparing mixture analysis results generated from a

historical mixture data set on the 3130xl Genetic Analyzer, using existing

AmpFlSTR® kits and analyzed using GeneMapper® ID-X Software version 1.1

and 1.2.

• Verification of features carried over into GeneMapper® ID-X Software Version 1.2

from GeneMapper® ID-X Software version 1.1.1 including:

– Adjustment of profile-comparison match-filtering to correct an issue where

some matches were not properly displayed

– Update to correct marker name sorting in the profile comparison display

– Changes to the CODIS export file format to remove the registered trademark

which could not be translated by Chinese operating systems

– Changes to the CODIS export file format to display September in 3 character

format (SEP)

– The option to turn allelic ladder spike detection on or off

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Verification

– Update to the local southern sizing algorithm to address a rarely observed

issue with GeneScan™ 600 LIZ™ Size Standard, in which size standard

fragments form a perfect line resulting in incorrect plot display and

abnormal peak morphology

– Address an issue where modifications to the size standard or analysis

method do not get exported when the project is exported (if the project is not

re-analyzed)

The test cases performed in this verification study and the passing criteria are

summarized in Table 2 on page 5. A summary of the verification study tests and results

is shown in Table 3 on page 7.

Sizing and

genotyping

concordance

A total of 2204 .fsa sample files were used in the concordance test. The sources and

types of samples are listed in Table 1 on page 4.

4GeneMapper® ID-X Software Version 1.2 User Bulletin:Verification Experiments and Installation Procedures

Verification Experiments and Installation Procedures

Verification

Table1 Number of sample files by type used in concordance testing. The capillary electrophoresis instrument, data collection software version, and AmpFlSTR®

kit used to generate data are noted.

Instrument/

Data Collection

Software

AmpFlSTR®

Kit

Single

Source Sensitivity Mixture

Low

Quality

DNA

LOR OMR Spike Good

Ladder

Low

Quality

Ladder

Positive Negative Total

ABI PRISM® 310

Genetic Analyzer

DC Software v3.0/

3.1

COfiler®16 6 5 12 8 1 1 49

Profiler Plus®16 6 6 12 8 1 1 50

Identifiler®16 6 6 12 15 1 1 57

SGM Plus®16 8 6 12 5 1 1 49

SEfiler®16 5 6 12 5 1 1 46

Yfiler®80 6 1 1 88

MiniFiler™30 7 1 1 39

3100 Genetic

Analyzer

DC Software v1.1/

2.0

COfiler®16 6 6 12 3 1 1 45

Profiler Plus®16 6 6 12 3 1 1 45

Identifiler®16 6 6 12 3 1 1 45

SGM Plus®16 6 6 12 3 1 1 45

SEfiler®16 6 6 12 3 1 1 45

3100-Avant

Genetic Analyzer

DC Software v2.0

COfiler®16 6 6 12 3 1 1 45

Profiler Plus®16 6 6 12 3 1 1 45

Identifiler®16 6 6 12 3 1 1 45

3130xl Genetic

Analyzer

DC Software v3.0

Profiler Plus®272 272

Identifiler®93 24 12 30 12 10 9 243 58 1 1 493

SGM Plus®32 32

SEfiler®16 16

Yfiler®10 2 16 32 1 1 62

MiniFiler™10 2 32 1 1 46

Sinofiler™113 36 14 140 6 309

3730 Genetic

Analyzer; DC

Software v3.0

Identifiler®90 48 96 1 1 236

Total 634 187 107 186 12 10 25 941 58 19 25 2204

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Verification

The data listed in Table 1 were analyzed using both GeneMapper® ID-X Software

versions 1.1 and 1.2. The Genotypes and Sizing tables were exported and compared

using Beyond Compare® Software version 2.2.3 to determine whether any differences

in data output resulted. The peak heights, peak areas, data points, sizes, allele names,

and all the PQV scores were compared between the two software versions.

Mixture analysis A historical mixture sample data set was used to validate the concordance of the

mixture analysis results between the GeneMapper® ID-X Software versions 1.1 and

1.2. The data set included 2-contributor mixtures made from 8 pairs of genomic DNA

samples prepared in 11 mixture ratios: 1:0, 1:1, 1:2, 1:3, 1:5, 1:9, 9:1, 5:1, 3:1, 2:1, and 0:1.

The mixture samples were amplified with the AmpFlSTR® Identifiler® (1 ng and

0.125 ng), Profiler Plus®, COfiler®, and SGM Plus® PCR Amplification kits. The

samples were analyzed on two 3130xl instruments. 476 sample files were used to test

Mixture Interpretation Threshold (MIT) pruning and sample segregation; 287 sample

files were used to compare pattern extraction; 31 sample files were used to test

Random Match Probability (RMP), Combined Probability of Inclusion/Exclusion (CPI/

CPE), and Likelihood Ratio (LR) calculations; 24 sample files were used for known

matching; 4 pairs of sample files were used to test multiple kit merging.

The following mixture analysis log files or exported files in .txt format were generated

on both versions of the software with the exact same population databases, MIT and

PHR thresholds, theta values, LR hypotheses, and conclusive options:

• SampleClassification_Log for MIT pruning and sample segregation.

• MixtureSamples and MixtureSamples (analysis log) for pattern extraction, PHR,

residual, Mx and Avg Mx calculation, as well as UI flags and values.

• MixtureSampleStatsCalc_Log for RMP, CPI, and known matching calculations.

• Individual MAData export files for RMP, CPI, LR, and multiple kit merging

calculations.

These text files were compared using the Beyond Compare® Software v2.2.3. The

comparison tool lined up the text files from the two software versions, and compared

the file contents for concordance.

Table2 Verification test cases

Tested Cases Test Description Passing Criteria

CODIS export removal of

registered trademark

Perform analysis of 10 single-source samples. After

genotyping analysis, export the CODIS tables.

Verify the registered trademark is

removed in CODIS export file.

CODIS export date

format

Perform analysis of 10 single-source samples from

projects created in September 2008. After genotyping

analysis, export the CODIS tables.

Verify September date is displayed as

SEP.

Profile Comparison

Match Display

Using a project displaying the issue, set the percent

match threshold to 90%, 80%, and 70%. Check the

accuracy of the profile match filtering.

Select one mixture sample set each from Identifiler®,

Profiler Plus®, SGM Plus®, and COfiler® kit data with

mixture ratios of 0:1, 1:1, 1:3, 1:5, 1:9, and 1:0. Set the

percent-match threshold to 90%, 80%, and 70%. Check

the accuracy of the matching entry filtering.

Verify all matches correctly displayed at

each match threshold.

Profile Comparison

Marker Order Display

Using test data above, verify matching entries displayed

in the same marker order.

Verify all matches correctly displayed

with marker order sorted by dye color

and size.

6GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Verification

Modified Size Standard/

Analysis Method Export

Select 3 single-source samples from a previously

analyzed project. Modify the size standard by adding or

deleting one or more peaks and click OK to save.

Without re-analyzing the data, export the project and

delete the project from GeneMapper Manager. Re-

import the project, open it, verify that the changed size

standard settings are persisted.

Select 3 single-source samples from a previously

analyzed project. Modify the Analysis Method by

changing any of the parameters and save. Without re-

analyzing the data, export the project and delete the

project from GeneMapper Manager. Re-import the

project, open it and verify that the changed Analysis

Method settings are persisted.

Verify that size standard changes are

persisted and can be exported with the

project.

Verify that analysis method changes are

persisted and can be exported with the

project.

Size Calling Issue Using a project displaying the issue, verify the issue is

corrected.

Select 5 single-source samples from previously

analyzed projects, including data generated on the 3730

with GS600, 31xx with GS500LIZ and 31xx with

GS500ROX. Re-analyze the data to verify no changes

happened due to this update.

Verify that the issue is corrected for a

sample that was previously affected.

Verify that all remaining data were

correct and unaffected by the update.

Y-scale Display Perform analysis of 10 single-source samples. After

genotyping analysis, zoom in on the y-scale of each

sample to view the y-scale values. Print the

electropherograms.

Verify that the Y-scale is printed

according to the plot settings.

Print Individual Sample

Followed by Page Break

Perform analysis of 10 single-source samples. After

genotyping analysis, print the profiles and genotype

tables with the page break.

Verify the page break is inserted properly.

Disable Spike Detection

for Allelic Ladders

Perform analysis of 10 single-source samples and 10

allelic ladders containing spikes. Genotype the data with

the spike detection for allelic ladder enabled and

disabled. Compare the allele calls, sizing, and PQV

results.

Verify that spikes are not detected in

allelic ladders when the spike detection

option is disabled.

Verify that spikes are detected in allelic

ladders when the spike detection option

is enabled.

Verify that spikes are detected in samples

when the Allelic Ladder spike detection

option is enabled and disabled.

Tested Cases Test Description Passing Criteria

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Verification

Table3 Summary of verification testing results

Results All metrics were met as described above and only one result of note was identified. In

one specific case, a labeling difference was found between the two software versions in

the size plot and table. This instance involved one AmpFlSTR® Sinofiler™ kit allelic

ladder sample which was deemed to be low-quality due to poor resolution (broad

peaks). Due to a broad peak, the 19.3 and 20 alleles in the D12 marker were labeled as a

single 19.3 allele and, in GeneMapper® ID-X Software version 1.1, shifted every

subsequent peak to the right. This resulted in labeling the first peak (allele 7) in D18 as

the last peak (allele 27) in D12. The OVL warning was properly triggered and the

Genotype Quality flag for the D12 marker was red, indicating that the ladder failed.

Testing Categories Subcategories Expected Outcome Result

Concordance with

GMID-X v1.1

Genotyping Allele calls 100% concordant, with ladder spike detection

on.

Pass

Peak Detection Size, height, area, and data point 100% concordant. Pass

PQVs With the ladder spike detection on, all the PQV scores,

including SQ values, shall be 100% concordant.

With the ladder spike detection off, the relevant PQV scores

are expected to have discrepancies.

Pass

Mixture Analysis All the mixture analysis calculations and flags shall be

100% concordant using the same analysis method and

thresholds.

Pass

Verification of patch

updates

Profile Comparison Incorrect

Result

The % match filter shall generate correct result – all

matching entries shall be displayed when the match

threshold is adjusted.

Pass

Profile Comparison Sorting of

Marker

The markers shall be sorted consistently across the

samples in the Profile Comparison view, first by dye then by

the size order.

Pass

CODIS Export - Date Format

Issue

For CODIS export, all months shall be three letters. The

September shall show up as “SEP”.

Pass

CODIS Export - Registered

Mark Issue

In the CODIS export, the registered trademark in the

software name shall be removed.

Pass

Start a New Page to Print for

each Sample

With the option to insert a page break after each individual

sample plot, the printout will show that each sample is

printed starting on a new page, even though one sample

may overflow to multiple pages.

Pass

8GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Verification

Two issues were observed:

1. In both v1.1 and v1.2 software, labeling of the first D18 peak in the sample plot

was not consistent with the allele name in the size table.

2. Labeling of the first D18 peak in the sample plot was different between v1.1 and

v1.2 software.

• In v1.1 software:

– The allele designation was 7 in the sizing table

– The allele designation was 27 in the sample plot

– When the peak in the sample plot was highlighted, the D18 row in the

sizing table was selected

• In v1.2 software:

– The allele designation was 27 in the sizing table

– The allele designation in the sample plot was 7

– When the peak in the sample plot was highlighted, the D12 row in the

sizing table was selected

In all cases, the contents of the genotypes table from v1.1 and v1.2 match each other.

This issue was not observed with any allelic ladders that passed all quality metrics.

Conclusions • The GeneMapper® ID-X Software Version 1.2 can be used to process .fsa files

generated on legacy capillary electrophoresis instruments and with existing

AmpFlSTR® kits.

• The same results for sizing, genotyping, and mixture analysis were obtained

using GeneMapper® ID-X Software versions 1.1 and 1.2.

• All updates to GeneMapper® ID-X Software Version 1.2 were successfully and

correctly implemented without deleterious effects on other software

functionality.

• A difference in handling overlapping marker data was observed between the two

software versions in one instance of a low-quality Sinofiler™ kit allelic ladder.

Based on the nature of the modifications addressed in this update, and the testing that

we performed, it is recommended that users evaluate this software as appropriate for

their laboratory workflow to demonstrate concordance to previously validated

GeneMapper® ID-X Software versions. Laboratories should determine the appropriate

level of testing required based on their internal software validation guidelines and

those of the appropriate governing agencies.

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

General

installation

instructions

Before installing the GeneMapper® ID-X Software Version 1.2, review the

GeneMapper® ID-X Software Version 1.2 Installation Guide and any supplemental

documentation.

General guidance to ensure successful GeneMapper® ID-X Software Version 1.2

installation or upgrade from previous GeneMapper® ID-X Software versions includes:

• Computer with 1 GB or higher memory is highly recommended

• Computer virtual memory should be set at Custom Size (2046-4092 MB) before

software installation or upgrade

• User must log in to the local computer and have Administrator privileges when

installing, un-installing, or upgrading the GeneMapper® ID-X Software Version

1.2

• When un-installing the GeneMapper® ID-X Software, the user should log in using

the same user name as the one used when installing the software to ensure clean

un-installation. User must follow the un-installation message prompts during un-

installation process.

• When performing an upgrade on a stand-alone computer, use the Add/Remove

Program feature in Microsoft Windows® to un-install the previous version of

GeneMapper® ID-X Software

About co-

installation with

Data Collection

Software

Computers with both GeneMapper® ID-X Software Version 1.2 and Data Collection

Software installed (referred to as a “co-installation”) should not be used as the host

computer for other clients. Such host computers can only connect to one client

computer.

When GeneMapper® ID-X Software Version 1.2 is not co-installed with Data

Collection Software, remote autoanalysis is supported for the 31xx platform, but not

for the 3500/3500xL and 310 platforms.

Perform a fresh

GeneMapper® ID-X

Software Version

1.2 installation

Use the GeneMapper® ID-X v1.2 Full or Client installation CD for fresh installations or

installations to computers installed with GeneMapper® ID Software v3.2. A detailed

description of different fresh installation scenarios and steps performed by the user is

summarized in Table 4 on pages 10 and 11.

10 GeneMapper® ID-X Software Version 1.2 User Bulletin:Verification Experiments and Installation Procedures

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

Table4 Fresh installation scenarios and steps

Installation

Type Scenario Choose CD Steps

GeneMapper®

ID-X Software

Version 1.2 Full

installation

GeneMapper® ID-X

Software Version 1.2

Full fresh

installation

Stand Alone Computer without any

version of GeneMapper® ID-X

Software or Data Collection Software

installed

GeneMapper®

ID-X v1.2 Full

PN 4443261

1. Log on to the local computer to an account with

Administrator privileges.

2. Insert the CD and follow the instructions.

Installation takes about 1 hour to complete.

3. Enter the Registration Code to launch the software.

Co-Install to a computer with Data

Collection Software installed#

GeneMapper® ID-X

Software Version 1.2

Full installation to a

computer installed

with GeneMapper®

ID Software v3.2§

Stand Alone Computer with

GeneMapper® ID Software v3.2

1. Log on to the local computer to an account with

Administrator privileges.

2. Insert the CD and follow the instructions.

The software detects the GeneMapper® ID Software v3.X.

3. Export the data objects (projects, analysis methods, etc.)

4. Uninstall the GeneMapper® ID Software v3.2 (Remove

Program).

5. Restart the computer, then log on to the local computer to

an account with Administrator privileges.

6. Install the GeneMapper® ID-X Software v1.2 Full.

7. Enter the Registration Code to launch the software.

Co-Install to a computer with

GeneMapper® ID Software v3.2 and

Data Collection Software installed#

GeneMapper® ID-X Software Version 1.2 User Bulletin:Verification Experiments and Installation Procedures

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

GeneMapper®

ID-X Software

Version 1.2

Client

installation‡

Fresh client

installation

Stand Alone Computer without any

version of GeneMapper® ID-X

Software installed

GeneMapper®

ID-X v1.2 Client

PN 4443260

1. Log on to the local computer to an account with

Administrator privileges.

2. Insert the CD and follow the instructions.

Installation takes about 15 minutes to complete.

3. Enter the Registration Code to launch the software.

Co-Install to a computer with Data

Collection Software installed#

Fresh installation to

a computer with

GeneMapper® ID

Software v3.2

Stand Alone Computer with GMID

v3.2 installed

1. Log on to the local computer to an account with

Administrator privileges.

2. Insert the CD and follow the instructions.

The software detects the GeneMapper® ID Software v3.X.

The installation stops because GeneMapper ID-X v1.2 Client

version does not have an export utility.

3. Insert a GeneMapper ID-X v1.2 full version CD to export the

data objects (projects, analysis methods, etc.).

4. Uninstall the GeneMapper® ID Software v3.2 (Remove

Program).

5. Restart the computer, then log on to the local computer to

an account with Administrator privileges.

6. Install the GeneMapper® ID-X Software v1.2 Client.

7. Enter the registration code to launch the software.

Co-Install to a computer with

GeneMapper® ID Software v3.2 and

Data Collection Software installed

‡ In order to use the Client version, a GeneMapper® ID-X Software Version 1.2 Full version must be installed on another computer first.

§ If GeneMapper® ID Software v3.1 is installed on the computer, uninstall v3.1 or upgrade to GeneMapper® ID v3.2 before installing GeneMapper® ID-X Software Version 1.2.

# GeneMapper® ID-X Software Version 1.2 can be co-installed with legacy Data Collection Software v2.0 and 3.0, 3500 Data Collection Software on Windows XP OS, or on 3500 Data Collection

Software on Windows Vista OS. However, co-installation with 3500 Data Collection Software is not recommended.

Installation

Type Scenario Choose CD Steps

12 GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

Upgrade from a

previous version

GeneMapper® ID-X Software versions 1.0, 1.0.1, 1.1, and 1.1.1 can be upgraded to

GeneMapper® ID-X Software Version 1.2 by using the GeneMapper® ID-X Software

Version 1.2 Full Upgrade or Client Upgrade CD.

1. Before upgrading the software, back up all GeneMapper® ID-X Software project

data and results, close all instances of previous versions of GeneMapper® ID-X

Software (on both Client and Full computers), and close all other software

programs and open windows.

2. During the installation of GeneMapper® ID-X Software Version 1.2 (in both fresh

installation and software upgrade cases):

a. Install the GeneMapper® ID-X Software Version 1.2 on the Full version first

and then on all Client versions which will connect to the Full version

database.

b. Allow the CD to Autostart, or double-click the Setup.exe file to launch the

installer.

3. After the software upgrade, enter the registration code obtained with the

previous software version to launch the software. The data analyzed from

previous GeneMapper® ID-X Software versions can be imported and viewed in

GeneMapper® ID-X Software Version 1.2.

A detailed description of different upgrade scenarios and steps performed by the user

is summarized in Table 5.

Table5 Upgrade scenarios and steps

Installation Type Scenario Choose CD Steps

Upgrade to

GeneMapper® ID-X

Software Version 1.2

Full

Upgrade GeneMapper® ID-X

Software v1.0, 1.0.1, or 1.1 Full

installed on a stand-alone

computer

GeneMapper® ID-X

v1.2 Full Upgrade

PN 4448530

1. Log on to the local computer to an

account with Administrator privileges.

2. Export the data objects (projects,

analysis methods, etc.)

3. Uninstall the previous version of

GeneMapper® ID-X Software (Remove

Program).

4. Restart the computer, then log on to the

local computer to an account with

Administrator privileges.

5. Insert the CD.

6. Install the GeneMapper® ID-X Software

v1.2 Full Upgrade.

7. Enter the Registration Code from the

previous version to launch the software.

Upgrade GeneMapper® ID-X

Software v1.0, 1.0.1, or 1.1

co-installed with Data

Collection Software

1. Log on to the local computer to an

account with Administrator privileges.

2. Insert the CD and follow the

instructions.

3. Enter the Registration Code from the

previous version to launch the software.

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

Please note:

• Upgrade all instances of previous versions of the GeneMapper® ID-X Software,

including both GeneMapper® ID-X Software Client and Full versions before

launching the GeneMapper® ID-X Software Version 1.2.

• Depending on your computer configuration, you may be able to directly upgrade

the previous version of GeneMapper® ID-X Software to version 1.2, or you may

need to completely uninstall the previous version before installing version 1.2.

For Full installations of v1.2, you must manually uninstall any previous versions.

For Client versions of v1.2, the installer software automatically performs the

upgrade when you insert the version 1.2 CD.

•If the GeneMapper

® ID-X Software is installed on a computer platform that has

not been validated by Applied Biosystems/Life Technologies, you may experience

difficulties during the software upgrade. Please review the list of recommended

software operating systems before beginning installation, and report any issues

encountered during installation to your local Human Identification support

specialist.

Troubleshooting Setup.exe errors during software installation

If you see the following error message, or any setup.exe error, when you install the

software, the virtual memory settings of your Windows operating system are

insufficient and need to be adjusted as follows:

1. Close the error message window and let the installation finish, then uninstall the

software using Add/Remove programs.

2. After uninstallation, adjust the virtual memory settings:

a. Right-click My Computer, then select Properties.

b. In the System Properties dialog, select the Advanced tab, then select

Performance Settings.

Upgrade to

GeneMapper® ID-X

Software Version 1.2

Client‡

Upgrade GeneMapper® ID-X

Software v1.0, 1.0.1, or 1.1

Client installed on a

stand-alone computer

GeneMapper® ID-X

v1.2 Client Upgrade

PN 4448531

1. Log on to the local computer to an

account with Administrator privileges.

2. Insert the CD and follow the

instructions.

The software detects the previous

version of GeneMapper® ID-X Software.

3. Enter the Registration Code from the

previous version to launch the software.

Upgrade GeneMapper® ID-X

Software v1.0, 1.0.1, or 1.1

Client co-installed with Data

Collection Software

‡ In order to use the Client version, a GeneMapper® ID-X Software Version 1.2 Full version must be installed on another computer first.

Installation Type Scenario Choose CD Steps

14 GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

c. In the Performance Options dialog, select the Advanced tab, then select

Virtual memory Change.

d. Select the drive that contains the Windows operating systems (usually the C:

drive), then select Custom size. Enter 2046 MB for Initial size and 4092 for

Maximum size.

Note: For some systems, any values larger than 500 MB may be sufficient.

e. Click Set, click OK, then click Apply.

3. Restart the computer, then return to the Virtual Memory dialog and confirm that

the Paging File Size is set for the drive that contains the Windows operating

system (usually the C: drive).

4. Install the GeneMapper® ID-X Software Version 1.2.

Note: If you receive an error at this stage indicating that Oracle is found on the system,

delete the X:\AppliedBiosystems\OracleGMIDX folder, restart the system, then

attempt another install. In more severe cases, you may need to run the

RemoveOracle.exe program found on the installation CD in the Tools folder.

GeneMapper® ID-X Software Version 1.2 User Bulletin

Verification Experiments and Installation Procedures

Installing GeneMapper® ID-X Software Version 1.2

Obtaining support For HID support:

• In the United States and Canada – send an email to

HIDTechSupport@lifetech.com, or call 888-821-4443 option 1.

• Outside the United States and Canada – contact your local support office.

For the latest services and support information for all locations, go to:

www.appliedbiosystems.com

At the Applied Biosystems web site, you can:

• Access worldwide telephone and fax numbers to contact Applied Biosystems

Technical Support and Sales facilities.

• Search through frequently asked questions (FAQs).

• Submit a question directly to Technical Support.

• Order Applied Biosystems user documents, SDSs, certificates of analysis, and

Thermo Fisher Scientific GeneMapper® ID-X Software Owner's manual

Brand: Thermo Fisher Scientific

Model: GeneMapper® ID-X Software

Type: Owner's manual

Download PDF

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Thermo Fisher Scientific GeneMapper® ID-X Software Owner's manual

Thermo Fisher Scientific GeneMapper® ID-X Software Owner's manual

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