Varian PROSTAR 210 Driver Manual

Varian, Inc.

2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA

©Varian, Inc. 2005 03-914947-20:Rev. 5

ProStar 210/215 and PrepStar

218/SD-1 Solvent Delivery

Module Control

Galaxie Driver Manual

ProStar 210/215/ PrepStar 218/SD-1 SDM -2- 03-914947-20:5

Table of Contents

Introduction.............................................................................................. 3

General ........................................................................................................................... 3

Installation and Configuration................................................................ 4

Installing the Driver Software .......................................................................................... 4

Configuring the Communication Server.......................................................................... 4

Configuring the Driver ..................................................................................................... 5

Configuring the ProStar / PrepStar Pump................................................................... 5

Configuring the Synopsis ............................................................................................9

Building a Method ................................................................................. 12

Creating a New Method ................................................................................................ 12

ProStar / PrepStar Pump Control Method .................................................................... 14

Elution Control........................................................................................................... 16

Miscellaneous Control............................................................................................... 18

Pressure Control........................................................................................................19

Injection Control.........................................................................................................20

Starting and Viewing an Acquisition.................................................... 22

Quick Start and Sequence ............................................................................................ 22

Quick Start (Single Acquisition).................................................................................22

Sequence .................................................................................................................. 23

Running Acquisition Status ........................................................................................... 24

Viewing a Running Acquisition..................................................................................24

Status of the ProStar / PrepStar Pump ..................................................................... 24

Appendix................................................................................................ 30

Pressure Limits (in bar)................................................................................................. 30

Compressibility and Pressure Constant........................................................................ 31

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Introduction

General

This document describes the configuration of the Varian ProStar

210, 215 and Varian PrepStar 218, SD-1 pump driver. It also

explains how to build a control method for this driver, how to start

and view a running acquisition and what actions are available

during an analysis.

The ProStar 210, 215 and PrepStar 218 Solvent Delivery

Modules are identical in appearance and operation. The ProStar

215 and PrepStar 218 have more powerful motors, larger power

supplies and can operate at higher pressures than the ProStar

210. The pressures are identified in the Appendix.

The manual will refer to the pumps as 210/215/218/SD-1

throughout.

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Installation and

Configuration

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie Data

System Installation Guide.

Configuring the Communication Server

To communicate with the ProStar 210-215-218-SD-1 module a

RS232_Interface or a RS232_PC communication bus must be

configured on the acquisition server.

The parameters of the bus to must be the following:

Stops

1 Stop

Baud 19200

Parity EVEN

Bits 8 Bits

Flow Control NONE

Buffer Tx 4096

Buffer Rx 4096

NOTE: For more details about the configuration of a RS232_Interface or a RS232_PC

bus, refer to the Galaxie Chromatography Data System Installation’s Guide.

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Configuring the Driver

The driver software configuration is done in the Galaxie

Configuration Manager when a new chromatographic system is

created.

Configuring the ProStar / PrepStar Pump

Click on the button corresponding to the ProStar /

PrepStar pump driver and the pump configuration screen

appears:

The toolbar allows you to easily scroll through the configuration

windows:

to select the first configuration window.

to select the previous configuration window.

to select the next configuration window.

to select the final configuration window.

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Communications Window

Select from the list the name of the RS422 Bus where the

system has been connected.

NOTE: For more details about the RS422 Bus configuration, refer to the section

Configuring the Communication Server in this manual.

Selection will not appear

until the RS422 BUS is

configured.

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Hardware Window

Select from the list the type of the pump: isocratic, binary,

ternary, quaternary, isocratic + injector, binary + injector, ternary

+ injector. A new tab (Pump B, Pump C, Pump D or Injector)

appears depending on the selected type of pump.

NOTE:

- The system must be equipped by at least two ProStar / PrepStar modules to work as a

binary pump, three modules for a ternary one and four to work in quaternary mode.

- With this type of pump, a module can be used as an injector. In this case a new

Injection screen becomes active in the method. To learn more about this option, refer to

the chapter

Control method for the ProStar / PrepStar pump.

- When an Injector module is configured, in the lower part of the Galaxie Configuration

Manager an injector (of the new system) called ProStar 210/215/218 pump is created.

The ID entered here

must match the ID of

the pump.

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Enter the identification number of the pump module in the RS-

422 unit ID field. The RS-422 unit ID field must match the ID

specified on the pump itself. To check the ID on the pump, press

Setup on the pump (no flow), scroll left until the ID is displayed.

Press Enter when finished.

Select from the list the model of the pump module. Four choices

are available: ProStar 210, 215, PrepStar 218 and SD-1.

The 210/215/218 modules are identical in appearance and

operation. They differ only in one respect: the ProStar 215 and

PrepStar 218 have more powerful motors, larger power supplies

and can operate at higher pressures than the ProStar 210.

NOTE: The pump head size must be configured on the instrument. Maximum flow rates

authorized in Galaxie will then depend on that configuration.

- With ProStar 210/215 and PrepStar 218: press the ~ button to access the setup menu

and enter the correct head size under Hdsz section using the ▲ and ▼ buttons.

- With the PrepStar SD-1: press the ~ button to access the setup menu. Select YES

under Change Pump using the ▲ and ▼ buttons and follow the procedure displayed in

the LCD screen. At the end of the procedure, new pump head size will be automatically

configured.

Please refer to the instrument manual for more details.

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Pressures Window

Select from the list the Pressure units. Three choices are

available: bar, PSI and kPa.

Configuring the Synopsis

In the Overview part of the system configuration, a synopsis can

be defined for the system. This synopsis depends on the

configured devices.

1. To save the configuration of these devices, click on the OK

button.

2. Right click on the system name in the bottom part of the

Galaxie Configuration Manager screen. A pop-up menu

appears: click on Stop. Then right click on the system name

again, and click on Start.

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3. Go back to the System Configuration screen (Properties

wizard). A graphic of the devices that comprise the system is

displayed in the synopsis screen. To configure the synopsis

as the real instrument configuration, move each module, and

then connect it appropriately. To do this, select one anchor

for each of the two modules (the selected anchors become

red) and click on the

button; a connection tube will

appear between the two anchors.

Click on the

button to delete a selected tube, click on

the

button to delete all the tubes.

Click on

to define a background color, or on to

make the background transparent.

Click on the

icon to change the grid color.

4. Once the synopsis configuration is correctly done, it will be

displayed in Galaxie Chromatography Data System, in the

Systems tab. In this tab, the Overview part will show the

synopsis of the system with some parameters displayed

(temperature, flow, signal, etc.) depending on the installed

devices.

For example, the following pictures represent the synopsis of

the ProStar / PrepStar pump:

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Galaxie Configuration Manager Screen

Galaxie Chromatography Data System Screen

NOTE: Once the system has been created and correctly configured, it must be

associated with one or more projects in order to start acquisitions. For more

details about the creation and configuration of a system, refer to the Galaxie

Configuration Manager User’s Guide (chapter The System).

Click on the

button to print the current configuration

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Building a Method

Creating a New Method

The method contains parameters for instrument control, data

acquisition, chromatogram processing, and editing of final

results. To initially set up a method, it is not necessary to define

all of the sub-method sections; however, you must fill in the

instrument control section prior to starting an acquisition.

1. To create a new method, select the FILE / NEW METHOD

option from the Galaxie Chromatography Data System main

menu.

A wizard guide is displayed to provide assistance during the

first steps of method creation:

2. Choose the system (chromatograph) associated with the

method. A method is created for a particular system. When

ProStar 210/215/ PrepStar 218/SD-1 SDM -13- 03-914947-20:5

starting an acquisition, the name of the system that performs

the acquisition must be correct, since method access is

limited only to those methods associated with this system.

3. Once this field is completed click on the Next button to move

to the second step of the method creation:

4. Enter the New Method Name in the first field. In the

Description field, enter information concerning the method

(optional).

5. Once these two fields have been completed, click on the OK

button. The new method is created and is opened with

default parameters in the Galaxie Chromatography Data

System. The next step is the definition of each of the method

sections.

The instrument control section must be defined in order to

start an acquisition. In the following part of this guide, only

the instrument control section of the method for the ProStar /

PrepStar pump is detailed. Other parameters such as

Injection Volume, Vial and Rack number could be necessary.

They are defined in the acquisition sub-method part.

NOTE: For more details about the programming of each method section, refer to the

Galaxie Chromatography Data System User’s Guide.

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ProStar / PrepStar Pump Control Method

Once the new method is created, select the control section in the

lower part of the browser.

The following screen appears:

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Press the button to display the System Control Method

Advanced Tools window:

to send the current method to the instrument (not

applicable to this driver)

to get the active method from the instrument (not

applicable to this driver)

to print the current method

Click on the button to display the ProStar / PrepStar

pump control method section. It contains up to four sub-sections:

Elution, Miscellaneous, Pressure and Injection. Click on the

required sub-section to define the corresponding parameters that

will be used during the chromatographic run. The toolbar allows

you to easily scroll through the control method windows:

to select the first window of the control method.

to move back to the previous window of the control

method.

to move forward to the next window of the control

method.

to select the final window of the control method.

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Elution Control

Select from the list(s) the solvent of each pump module. Here

are listed all the available solvents:

Water Acetone

Acetonitrile Benzene

Carbon tetrafluoride Chloroform

Cyclohexane Dichloroethane

Diethyl Ether Dimethylformamide

Dioxane Ethanol

Ethyl Acetate Methylene Chloride

Methanol n-heptane

n-hexane o-dichlorobenzene

Propanol Tetrahydrofuran

Toluene 2-Methylformamide

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A compressibility factor and a pressure constant are associated

with each solvent (Compressibility and Pressure Constant fields).

When the Compressibility compensation field is unchecked, the

pump will not take into account the corresponding factors and

the factors will be concealed.

Refer to the Compressibility and Pressure Constant section in

the appendix of this manual to find the list of these values.

The refill time parameter is the total time that it takes to refill the

pump head with mobile phase. This value may be changed

when a very viscous mobile phase is used. In this case, a rapid

refill might lead to cavitations or bubble formation in the pump

head causing a lower than expected flow rate. If the refill time is

too long, excessive pulsations in the flow may be observed. This

parameter is programmable by pump module.

NOTE: The activation of the scrolling lists A, B…D depends on the type of the pump

previously configured in the Galaxie Configuration Manager (isocratic, binary,

ternary or quaternary pump).

Program a flow and/or a composition gradient. Each step of the

program includes time, flow and/or composition new values. The

Pumps Gradient Chart represents the evolution of these

parameters.

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

Below the pump gradient chart, select the display type of the

chart. Two choices are available Solvent A on top or Solvent A

on bottom.

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Miscellaneous Control

Start mode:

In the scrolling list, select the way to start the pump.

If immediately when ready is chosen, the pump will start as soon

as it is ready.

If inject trigger on pump X is chosen, the pump will start when an

inject marker is send to pump X.

End of Sequence:

In the scrolling list, select either to stop or not the pump at the

end of the analysis (Quick Start or sequence).

If Leave Pump On is selected, the pump will continue to work at

the last time-table programmed flow and composition.

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Pressure Control

Pressure Limits Parameter Group:

Enter the minimum and maximum pressure limits of the pump.

Outside these limits, the pump will stop and an alarm will sound.

NOTE:

- The maximum pressure limit of a ProStar / PrepStar module depends on the type of its

pump head. This limit is automatically adapted according to the connected pump head.

To know these limits, refer to the Pressure Limits chapter in the appendix of this manual.

- If a higher value of pressure is entered by error, a message appears indicating the

pressure limits for the connected pump head and the last entered valid value is

displayed.

- If several ProStar / PrepStar modules are connected (in case of binary, ternary or

quaternary pump), the maximum pressure limit is the lowest value among the different

pump ones.

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Injection Control

This window becomes active only when a pump module is

configured as an injector in the system (for more details refer to

the Configuration of the ProStar / PrepStar pump chapter).

Thru Pump Injection Parameter Group:

Enter the time when the injection will start in the Injection time

field.

Enter the flow rate of the injection in the Injection Rate field. The

end time of the injection is determined according to the flow rate

and the injection volume entered in the Acquisition method

section (or in the Quick Start window or in the Sequence).

NOTE: The Injection Volume entered in the Acquisition part of the method must be

entered in mL only.

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