Thermo Fisher Scientific Sequazyme™ DNA Standards Kit Operating instructions

Brand: Thermo Fisher Scientific

Model: Sequazyme™ DNA Standards Kit

Type: Operating instructions

Operating Instructions

1 Product Description

The Sequazyme™ DNA Standards Kit allows you to do either or both of

the following:

•Test the MassGenotyping Solution 1™ (MGS1) System,

excluding the SymBiot® XT Workstation

•Check and optimize the resolution and sensitivity of the

Voyager™ Biospectrometry™ Workstation (linear mode), used

alone or as part of the MGS1 System

The kit contains a mixture of three oligonucleotides that simulate the

products of a primer extension reaction run using the Sequazyme

Pinpoint SNP Assay Kit. The three oligonucleotides produce a mass

spectrum that simulates a primer and its A/T heterozygote extensions.

Figure 1 illustrates how to use the DNA Standards Kit.

Figure 1 Using the DNA Standards Kit

2 Instrument Safety

Before using the Voyager Workstation, read the Safety and

Compliance section in the Voyager Biospectrometry Workstation User

Guide and be familiar with all instrument safety information.

Before using the MGS1 System, read the Safety and EMC Compliance

section in the MGS1 System Hardware Guide and be familiar with all

instrument safety information.

Apply Matrix and Primer Extension Mixture

to a Voyager Sample Plate

Prepare the THAP Matrix

Prepare the Primer Extension Mixture

Test the MGS1 System Check and Optimize

Voyager Workstation

Resolution and Sensitivity

3 Materials

Materials Provided

The DNA Standards Kit contains:

•THAP Matrix – 2, 4, 6 trihydroxyacetophenone, 1 vial (40 mg)

•Matrix Diluent – 2 vials (1.5 mL each)

•Primer Extension Mixture – 1 vial (25 µg), containing:

Materials Not Provided

To use this kit, you need a:

•Fresh 200-µL MicroAmp® reaction tube with cap

•Voyager sample plate

NOTE: If you use this kit to test the MGS1 System, do not use a

96×2-position, flat, hydrophobic plastic surface plate. Use only a

sample plate supported by the MGS1 software, such as:

• 96-position, barcoded

• 100-position

• 384-position

• 384-position, barcoded

• 400-position

Oligo-

nucleotide Sequence

(5’ to 3’)

Concentration

When

Dissolved in

25 µL Water Mass

(Da)

R377-16 5’-CTT TGT TCT GGG TTT C-3’2 µM 4,860

R377-17A 5’-CTT TGT TCT GGA TTT CA-3’1 µM 5,156

R377-17T 5’-CTT TGT TCT GGA TTT CT-3’1 µM 5,147

Contents Page

1 Product Description ........................................................ 1

2 Instrument Safety............................................................ 1

3 Materials ......................................................................... 1

4 Preparing the THAP Matrix ............................................ 2

5 Preparing the Primer Extension Mixture ......................... 2

6 Applying Matrix and Primer Extension Mixture to the

Voyager Sample Plate .................................................... 2

7 Testing the MGS1 System .............................................. 3

8 Checking and Optimizing Resolution and Sensitivity...... 5

9 Troubleshooting.............................................................. 7

10 Storing the Kit ................................................................. 7

11 Accessories, Spare Parts, and Ordering Information...... 7

12 Technical Support........................................................... 7

Sequazyme™

DNA Standards Kit

4 Preparing the THAP Matrix

WARNING: CHEMICAL HAZARD. Matrix Diluent (with acetonitrile)

is a flammable liquid and vapor. It may cause eye, skin, and respiratory

tract irritation, central nervous system depression, and heart, liver, and

kidney damage. Please read the MSDS and follow the handling

instructions. Wear appropriate protective eyewear, clothing, and

gloves.

WARNING: CHEMICAL HAZARD. 2,4,6 trihydroxyacetophenone

(THAP) may cause eye, skin, and respiratory tract irritation. Please

read the MSDS and follow the handling instructions. Wear appropriate

protective eyewear, clothing, and gloves.

Preparing

CAUTION: To prevent contamination, wear gloves when performing

the following steps, do not touch the internal surfaces of the vials or

caps, and do not allow the underside of the caps to touch any surfaces.

To prepare the THAP matrix:

1. Transfer the contents of one of the vials of matrix diluent to the vial

of THAP matrix powder.

2. Cap and vortex the mixture for 5 to 10 seconds.

3. Allow any undissolved matrix to settle.

Matrix Stability

Protect THAP matrix powder from high temperatures and light. Discard

THAP matrix solution if it turns dark brown or fails to crystallize. For

more information, see Section 10, Storing the Kit.

5 Preparing the Primer Extension Mixture

WARNING: CHEMICAL HAZARD. Matrix Diluent (with acetonitrile)

is a flammable liquid and vapor. It may cause eye, skin, and respiratory

tract irritation, central nervous system depression, and heart, liver, and

kidney damage. Please read the MSDS and follow the handling

instructions. Wear appropriate protective eyewear, clothing, and

gloves.

WARNING: CHEMICAL HAZARD. The toxicological properties of

Primer Extension Mixture have not been thoroughly investigated.

Use appropriate precautions. Read the MSDS.

Preparing

CAUTION: To prevent contamination, wear gloves when performing

the following steps, do not touch the internal surfaces of the vials or

caps, and do not allow the underside of the caps to touch any surfaces.

To prepare the primer extension mixture:

1. Immediately before use, transfer 25 µL matrix diluent to the vial of

primer extension mixture.

2. Cap and vortex the mixture for 5 to 10 seconds.

Primer Extension Mixture Stability

The reconstituted primer extension mixture is stable for approximately

3 days at room temperature. For more information, see Section 10,

Storing the Kit.

6 Applying Matrix and Primer Extension

Mixture to the Voyager Sample Plate

6.1 Overview

The following sections describe how to manually spot the THAP matrix

and primer extension mixture on a Voyager sample plate.

NOTE: If you use this kit to test the MGS1 System, do not use a

96×2-position, flat, hydrophobic plastic surface plate. Use only a

sample plate supported by the MGS1 software, such as:

• 96-position, barcoded

• 100-position

• 384-position

• 384-position, barcoded

• 400-position

6.2 Cleaning the Sample Plate

WARNING: CHEMICAL HAZARD. Acetonitrile is a flammable liquid

and vapor. It may cause eye, skin, and respiratory tract irritation,

central nervous system depression, and heart, liver, and kidney

damage. Please read the MSDS and follow the handling instructions.

Wear appropriate protective eyewear, clothing, and gloves.

CAUTION: Do not scrub or sonicate the Voyager sample plate.

Scrubbing or sonicating can damage the coating on the plate.

Before spotting, clean the sample plate:

1. Rinse the sample plate with deionized or Milli-Q® (not distilled)

water to remove previous samples and contaminating salts.

2. If the sample plate contains analytes or matrixes that are not water

soluble, rinse the plate with acetonitrile, then rinse with deionized

or Milli-Q water.

3. Allow to air-dry.

6.3 Spotting the Sample Plate

CAUTION: To prevent contamination, wear gloves when performing

the following steps, do not touch the internal surfaces of the vials or

caps, and do not allow the underside of the caps to touch any surfaces.

To spot the sample plate:

1. In a fresh 200-µL MicroAmp® tube, mix 5 µL THAP matrix solution

with 5 µL reconstituted primer extension mixture.

2. Spot two or three positions on the Voyager sample plate with the

50:50 THAP matrix:reconstituted primer extension mixture. The

volume you spot depends on the sample plate you use:

3. Dry the plate in either of the following ways:

•Air-dry at room temperature for a few minutes.

•Place in a stream of gently flowing air, for example, in the

airstream of a fume or laminar-flow hood.

IMPORTANT: Do not use the air supply at a lab bench to dry the

plate unless the air supply is free of oils and additives.

Sample Plate Spot Volume (µL)

384- and 400-position sample plates 0.4 to 0.5

96- and 100-position sample plates 1.0

6.4 Sample Stability

Dried samples on a sample plate are stable for approximately one day.

Protect the plate from light if you do not analyze the samples within an

hour of spotting.

7 Testing the MGS1 System

7.1 Overview

The following sections describe how to use the DNA Standards Kit to

check that the MGS1 System (excluding the SymBiot XT Workstation)

is operating properly and has instrument settings optimized to

successfully identify alleles. It includes:

•Before you begin

•Creating a primer

•Creating a sample set

•Acquiring and processing data

•Viewing results

7.2 Before You Begin

Before you begin, complete the steps in:

•Section 4, Preparing the THAP Matrix

•Section 5, Preparing the Primer Extension Mixture

•Section 6, Applying Matrix and Primer Extension Mixture to the

Voyager Sample Plate

7.3 Creating a Primer

To create a primer:

1. Open the MGS1 software by double-clicking .

2. From the View menu, select Primers and Pools.

3. Click to display the Primer Browser.

4. If the R377-16 DNA Standard primer already exists in the Primer

Browser, go to Section 7.4. If not, continue with step 5.

5. In the Primer section at the top of the screen, click New.

6. For the Description, type DNA Standards Kit Primer.

7. In the Primer Sequence (5’ to 3’) text box, type:

CTTTGTTCTGGGTTTC

A calculated mass of 4860.27 appears in the Mass text box.

8. In the Expected Base Added to Primer text box, type AT.

9. Click Save. In the Primer Browser, type R377-16 DNA Standard

for the Name, then click Save.

7.4 Creating a Sample Set

To create a sample set:

1. In the MGS1 software, select Sample Set from the View menu.

2. At the top of the Sample Set screen, click New, then select the

following general information:

3. In the Select MALDI Plates section, click to display the Plate

Browser.

4. In the Plate Browser, click to display the Plate Editor.

5. In the Plate Name text box, type DNA Standard Plate, click OK,

then click OK again.

6. In the Set All Plates tab, click Add All to Sample List to add the

DNA Standard Plate you created in step 5 to the Sample Set List.

7. In the Sample Set List, type or select the following information for

the positions that contain the primer extension mixture.

8. Click-drag to select all plate positions that you did not spot, then

click above the Sample Set List.

9. In the Analysis Setup section, click Acquisition Method.

10. In the Acquisition Methods dialog box, select DNA Standard Kit

from the Acquisition Method Name list.

The DNA Standard Kit Acquisition Method specifies:

•Data File Directory – Voyager Computer Name\MGS1\Data

•BIC File Name – Voyager Computer Name\MGS1\

BIC_SET_Files\DNAStdKit.BIC or DNAStdKitPRO.BIC

NOTE: Open the DNAStdKit.BIC or DNAStdKitPRO.BIC file in the

Voyager Instrument Control Panel and verify the Control Mode is

set to Automatic. If it is not, change the Control Mode to

Automatic, then save the file.

11. Click OK.

12. In the Analysis Setup section, click Processing Method.

13. In the Processing Methods dialog box, select DNA Standard Kit

from the Processing Method Name list.

The DNA Standard Kit Processing Method specifies the settings

shown in Table 1 on page 4.

14. Click OK.

Parameter Setting

Sample Origin MALDI Plate

MALDI Plate Type (Select the MALDI Plate Type that matches

the plate you spotted in Section 6, Applying

Matrix and Primer Extension Mixture to the

Voyager Sample Plate.)

Parameter Selection

Sample Name DNA Standard

Single Primer Select

Primer/Pool Name R377-16 DNA Standard

Table 1 DNA Standard Processing Method Settings

15. Click Save at the top left of the Sample Set screen, type DNA

Standard Sample Set in the Sample Set Selection text box, then

click OK.

7.5 Acquiring and Processing Data

Selecting an Acquisition Mode and Sample Plate

To select an acquisition mode and sample plate:

1. If the Control Panels are not displayed in the MGS1 software,

select Control Panels from the View menu.

2. In the Data Acquisition Control Panel, select Acquire and Process

Data for the Mode.

3. Click to display the Select Plate dialog box.

4. Select DNA Standard Plate, then click OK to add it to the Data

Acquisition Control Panel.

Loading the Sample Plate in the Voyager™ Workstation

WARNING: LASER HAZARD. Lasers emit ultraviolet radiation.

Lasers can burn the retina and leave permanent blind spots. Never

look directly into the laser beam. Remove jewelry and other items that

can reflect the beam into your eyes. Do not remove the instrument

front or side panels. Wear proper eye protection and post a laser

warning sign at the entrance to the laboratory if the front or side panels

are removed for service.

To load the sample plate in the Voyager Workstation:

1. Power up the Voyager Workstation and start the Instrument Control

Panel.

2. In the Data Acquisition Control Panel, click .

A message prompts you to load the sample plate in the Voyager

mass spectrometer.

3. In the Voyager Instrument Control Panel, select Eject from the

Sample Plate menu.

4. In the Load/Eject dialog box, click Eject to eject the plate holder.

CAUTION: Verify that the sample plate is completely dry before

loading. Loading a wet plate can cause vacuum errors and

instrument damage.

5. Slide the sample plate into the holder from the right side with the

slanted underside of the plate facing to the left and toward the back

of the instrument, then snap the plate into place.

6. From the Sample Plate menu, select Load to retract the sample

plate and insert it into the main source chamber.

7. Select a Plate ID with a .PLT file that corresponds to the Voyager

sample plate you used to spot the THAP matrix and primer

extension mixture.

8. Click Load to load the sample plate. Wait for the Load/Eject Status

dialog box to close.

9. Align the sample plate by selecting Align from the Sample menu.

For more information on aligning the sample plate, see the MGS1

System Hardware Guide.

Starting Acquisition

After the Load/Eject Status dialog box closes in the Voyager software,

return to the MGS1 computer and click OK.

CAUTION: Do not click OK until the Load/Eject Status dialog box

closes. If you click OK before the mass spectrometer completes the

Load/Eject cycle, invalid results can occur.

7.6 Viewing Results

To view results after acquisition is complete:

1. In the MGS1 software, open the DNA Standard Sample Set by

selecting Sample Set from the View menu, clicking to display

the Sample Set Browser, selecting DNA Standard Sample Set,

then clicking OK.

2. Click the Result Viewer tab.

3. Select DNA Standard Plate from the Plate list, then select

R377-16 DNA Standard from the Primer list.

The results for the DNA Standard Plate and the R377-16 DNA

Standard primer are displayed in the Result Viewer tab (Figure 2).

Figure 2 Results of DNA Standard Plate and

R377-16 DNA Standard Primer

Parameter Setting

Processing Settings

Smoothing Methods Noise Filter

Correlation Factor 0.7

Baseline Correction Advanced

Data Explorer SET File Voyager Computer Name\

MGS1\BIC_SET_Files\

DNAStdKit.SET

Calibration Settings

External Calibration File (Not applicable, leave blank)

Peaks for internal calibration Primers Only

Relative Peak Intensity (%) 20

Mass Tolerance (Da) 15

Max Outlier Error (m/z) 2

Base Calling Settings

Allele Tolerance (Da) 1.5

Heterozygote Threshold (%) 20

Green = A

Red = T

4. Check that the positions on the plate that contain the THAP matrix

and primer extension mixture are green and red, indicating an

A/T heterozygote for the extended primers.

5. Click a position on the plate that contains the THAP matrix and

primer extension mixture to display the Results List (Figure 3).

Figure 3 Results List for Primer Extension Mixture

If your results do not indicate an A/T heterozygote, see Section 9,

Troubleshooting.

8 Checking and Optimizing Resolution

and Sensitivity

8.1 Overview

The following sections describe how to check and optimize resolution

on the Voyager Workstation to optimize it for DNA analysis. It includes:

•Before you begin

•Optimizing the Voyager Workstation (laser intensity)

•Checking resolution and sensitivity

•Resolution requirements

8.2 Before You Begin

Before you begin, complete the steps in:

•Section 4, Preparing the THAP Matrix

•Section 5, Preparing the Primer Extension Mixture

•Section 6, Applying Matrix and Primer Extension Mixture to the

Voyager Sample Plate

8.3 Optimizing the Voyager Workstation

(Laser Intensity)

WARNING: LASER HAZARD. Lasers emit ultraviolet radiation.

Lasers can burn the retina and leave permanent blind spots. Never

look directly into the laser beam. Remove jewelry and other items that

can reflect the beam into your eyes. Do not remove the instrument

front or side panels. Wear proper eye protection and post a laser

warning sign at the entrance to the laboratory if the front or side panels

are removed for service.

Before checking resolution and sensitivity, optimize the Voyager

Workstation settings from the Instrument Control Panel:

1. Power up the Voyager Workstation and start the Instrument Control

Panel.

CAUTION: Verify that the sample plate is completely dry before

loading. Loading a wet plate can cause vacuum errors and

instrument damage.

2. Load the sample plate in the Voyager Workstation. For the Plate

Type, select or create a Plate ID with a .PLT file that corresponds to

the Voyager sample plate you used to spot the THAP matrix and

primer extension mixture in Section 6, Applying Matrix and Primer

Extension Mixture to the Voyager Sample Plate.

3. Click in the toolbar of the Instrument Control Panel to turn on

the high-voltage power supplies. Allow the high-voltage power

supplies to warm up for 30 minutes for maximum mass accuracy.

4. From the Voyager Workstation Instrument Control Panel, open the

DNAStdKit.BIC or DNAStdKitPRO.BIC file, located on the

Voyager computer in the D:\MGS1\BIC_SET_Files subdirectory.

(Open the .BIC file appropriate for your instrument, Voyager-DE™

or Voyager-DE™ PRO Workstation.) Verify the Control Mode is set

to Manual.

If you do not have an MGS1 System, create the DNAStdKit.BIC or

DNAStdKitPRO.BIC file using the settings shown in Table 2.

Table 2 Instrument Settings for DNAStandardsKit.BIC File

Red = T

Green = A

Results of

Primer

Extension

Mixture

Parameter

Setting for

Voyager-DE

Workstation

Setting for

Voyager-DE PRO

Workstation

Instrument Mode Linear Linear

Extraction Type Delayed Delayed

Polarity Type Positive Positive

Linear Digitizer (Acqiris)

Bin Size 2 nsec 2 nsec

Vertical Scale 1000 mV 500 mV

Vertical Offset 0.0 0.0

Input Bandwidth Full Full

Control Mode Manual Manual

Accelerating Voltage 20,000 V 20,000 V

Grid Voltage% 94.5% 94.0%

Guide Wire Voltage% 0.05% 0.15%

Extraction Delay Time 300 nsec 350 nsec

Laser Shots/Spectrum 50 50

Acquisition Mass Range 3,000 to 7,000 Da 3,000 to 7,000 Da

Low Mass Gate 3,000 Da 3,000 Da

Calibration Matrix THAP THAP

Calibration File Default Default

5. In the Manual Laser Intensity section of the Instrument Control

Panel, set the laser intensity to 2,000.

6. Use the Manual Sample Positioning section of the Instrument

Control Panel to select a position on the sample plate containing

the THAP matrix and primer extension mixture.

7. Start acquisition by clicking on the toolbar.

8. During acquisition, view the spectrum in the Instrument Control

Panel. Verify that it looks similar to Figure 4.

Figure 4 Spectrum of Primer Extension Mixture

9. Increase the laser intensity in 50-step increments and observe the

signal intensity of the 4,860 Da peak.

You typically see major changes in signal intensity between laser

intensity settings of 2,000 and 2,100. At a laser intensity setting

between 2,100 and 3,000, you typically see a plateau in which

changes in signal intensity do not occur when you increase the

laser intensity.

10. Continue increasing the laser intensity in 50-step increments until

the signal intensity starts to decrease and you see peak

broadening and fragmentation.

The optimum laser intensity setting is midway between the

intensity that yields the signal intensity plateau and the intensity

that causes peak broadening and fragmentation (Figure 5).

Figure 5 Determining Optimum Laser Intensity

11. With the laser intensity at its optimum setting, save the

DNAStandardsKit.BIC file.

The laser intensity setting is saved with the .BIC file.

For more information on optimizing settings, see the Voyager

Biospectrometry Workstation User Guide, Section 5.1.4, Modifying an

Instrument Settings File (.BIC).

8.4 Checking Resolution and Sensitivity

To check resolution and sensitivity:

1. Optimize the Voyager Workstation settings as described in

Section 8.3, Optimizing the Voyager Workstation (Laser Intensity).

2. In the Data Storage section of the Instrument Control Panel,

specify D:\Voyager\Data as the directory for storing the data.

3. Use the Manual Sample Positioning section of the Instrument

Control Panel to select a position on the sample plate containing

the THAP matrix and primer extension mixture.

4. With the DNAStandardsKit.BIC or DNAStandardsKitPRO.BIC

file open, start acquisition by clicking in the toolbar. Acquire

five spectra from different regions of the same spot, saving each

spectrum to a data file.

5. From the Tools menu in the Voyager Workstation Instrument

Control Panel, use the Resolution Calculator and the

Signal-to-Noise Calculator to calculate the resolution and

signal-to-noise ratio of the unextended primer peak (4,860 Da) in

all five data files, then average the results.

NOTE: When calculating signal-to-noise ratios, specify a Baseline

Region that is flat (non-rising) and does not include peaks.

6. Check that the resolution is adequate to support your application.

See Table 3. The signal-to-noise ratio is typically 50:1.

7. If resolution is not adequate, do any of the following:

•Adjust laser intensity as described in Section 8.3, Optimizing

the Voyager Workstation (Laser Intensity).

•Adjust Delay Time in 50-nsec increments.

•Adjust Guide Wire Voltage% in 0.05% increments.

NOTE: Adjusting laser intensity and Delay Time to optimize

resolution typically also improves the signal-to-noise ratio.

8.5 Resolution Requirements

The instrument resolution required to resolve allelic pairs is a function

of the mass of the oligonucleotide containing the two alleles. The mass

of the oligonucleotide is proportional to its length (number of bases).

See Table 3.

Table 3 Maximum Primer Length (Number of Bases)

Supporting Resolution of Allelic Pairs

Table 3 serves as a guide for determining if the resolution of your mass

spectrometer is adequate to support a given application. For example,

at a measured resolution of 500 (m/∆m), the longest primer containing

a C/T heterozygote that can be resolved is 24 bases long. If the allelic

pair to resolve is unknown, assume you need a resolution that can

resolve the allelic pair with the lowest mass difference (A/T).

Unextended

primer peak Primer

extension

Depurination

peaks

Laser Intensity

Signal

Intensity

Major changes in signal

intensity occur with minor

laser setting adjustments

~2000 ~2500

Plateau, no changes in

signal intensity occur with

laser setting adjustments

Peak broadening

and fragmentation

occur at higher

laser settings

Optimum setting midway between

plateau setting and setting that

causes peak broadening and

fragmentation

Allelic Pair ∆ Mass

Resolution

400 500 600 700

A/C 24.03 31 39 47 55

A/G 16.00 21 26 31 36

A/T 9.01 12151820

C/G 40.03 52 65 78 91

C/T 15.02 20 24 29 34

G/T 25.01 31 39 47 55

NOTE: Primers over 30 bases in length are not recommended, even if

resolution requirements would support their use. Instrument response

decreases with longer primers.

9 Troubleshooting

Use Table 4 to troubleshoot problems you may encounter when testing

the MGS1 System.

Table 4 Troubleshooting Testing of the MGS1 System

10 Storing the Kit

The DNA Standards Kit is most stable at –20 °C. Kit components are

less stable at higher temperatures (see table below). Avoid prolonged

exposure to light.

11 Accessories, Spare Parts,

and Ordering Information

To order accessories and spare parts, contact Applied Biosystems

Customer Service. Refer to the back page of this document for the

phone number and web address.

12 Technical Support

For technical assistance, call Applied Biosystems Technical Support.

From North American, dial 1.800.899.5858. If you are outside North

America, refer to the back page of this document for the phone number

and web address.

Symptom Possible Cause Action

No result in the

Result Viewer tab

of the MGS1

software.

The mass scale of

the Voyager

Workstation is not

calibrated.

1. Open the data file in the

Data Explorer software.

2. Create an external

calibration file (.CAL).

3. In the MGS1 software

open the DNA Standard

Processing Method and

select this external

calibration file (.CAL).

4. Reprocess the data.

The results in the

Result Viewer tab

of the MGS1

software indicate a

single allele (an A

or T homozygote).

The resolution or

signal-to-noise

ratio of the

Voyager

Workstation is not

optimized.

Adjust laser intensity and

Delay Time to improve

resolution and sensitivity.

See Section 8, Checking

and Optimizing Resolution

and Sensitivity.

The Heterozygote

Threshold (%)

setting in the

Processing Method

is too high.

Decrease the

Heterozygote Threshold

(%) setting.

Kit Component Storage Temperature Stability

Unopened kit –20 °C 1 year

THAP Matrix (powder) –20 °C 1 year

Room temperature 1 month

THAP Matrix

(reconstituted) –20 °C 1 week

Room temperature 1 day

Matrix Diluent –20 °C 1 year

Primer Extension

Mixture (dried) <4 °C 1 year

Primer Extension

Mixture (reconstituted) –20 °C 1 month

4 °C 1 week

Room temperature 3 days

Description Quantity Part

Number

Sequazyme DNA Standards Kit 1 kit 4328119

Sequazyme Pinpoint SNP Assay Kit 1 kit, provided in

four separate

boxes

4315924

Voyager sample plate, hydrophobic

plastic surface, flat, 384-position 1 plate V700700

Voyager sample plate, hydrophobic

plastic surface, flat, 384-position,

barcoded

1 plate 4327695

Headquarters

850 Lincoln Centre Drive

Foster City, CA 94404 USA

Phone: +1 650.638.5800

Toll Free (In North America): +1 800.345.5224

Fax: +1 650.638.5884

Worldwide Sales and Support

Applied Biosystems vast distribution and

service network, composed of highly trained

support and applications personnel, reaches

into 150 countries on six continents. For sales

office locations and technical support, please

call our local office or refer to our web site at

www.appliedbiosystems.com.

www.appliedbiosystems.com

Applera Corporation is committed to providing

the world’s leading technology and information

for life scientists. Applera Corporation consists of

the Applied Biosystems and Celera Genomics

businesses.

Printed in the USA, 04/2001

Part Number 4327553 Rev. A

For Research Use Only. Not for use in diagnostic procedures.

Information in this document is subject to change without notice.

Applied Biosystems assumes no responsibility for any errors that

may appear in this document. This document is believed to be

complete and accurate at the time of publication. In no event shall

Applied Biosystems be liable for incidental, special, multiple, or

consequential damages in connection with or arising from the use of

this document.

Applied Biosystems, SymBiot, and MicroAmp are registered

trademarks of Applera Corporation or its subsidiaries in the U.S. and

certain other countries.

AB (Design), Applera, Biospectrometry, MassGenotyping Solution 1,

Sequazyme, Voyager, and Voyager-DE are trademarks of

Applera Corporation or its subsidiaries in the U.S. and certain other

countries.

Milli-Q is a registered trademark of Millipore Corporation.

All other trademarks are the sole property of their respective owners.

Thermo Fisher Scientific Sequazyme™ DNA Standards Kit Operating instructions

Brand: Thermo Fisher Scientific

Model: Sequazyme™ DNA Standards Kit

Type: Operating instructions

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Thermo Fisher Scientific Sequazyme™ DNA Standards Kit Operating instructions

Thermo Fisher Scientific Sequazyme™ DNA Standards Kit Operating instructions

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