RBCBioscience MagCore Super Training manual

MagCore®

Super/HF16 Plus

Engineer In Training

manual

1

Handle the power cord carefully.

Hold the plug when unplugging the cord.

Keep the set free from moisture, water, and dust.

Unplug the power cord when not using the set for

long periods of time.

Do not obstruct the ventilation space.

Do not let foreign objects in the set.

Do not let insecticides, benzene, and thinner come in

contact with the set.

Never disassemble or modify the set in any way.

Avoid high temperatures.

Allow for sufficient heat dispersion when installed on a rack.

CAUTIONS

Risk Of Electric Shock Do Not Open

Safety Precautions

Before use

These WARNINGS and CAUTIONS are intended to protect you and other persons from injuries and damages. To ensure safe operation, please follow them carefully.

CAUTION:

To Reduce The Risk Of Electric Shock, Do Not Remove Cover (Or Back).

No Userserviceable Parts Inside. Refer Servicing To Qualified Service Personnel.

CAUTION:

1. Handle the power supply cord carefully

Do not damage or deform the power supply cord. If it is damaged or deformed, it

may cause electric shock or malfunction when used. When removing from wall

outlet, be sure to remove by holding the plug attachment and not by pulling the

cord.

2. Do not open the top cover

In order to prevent electric shock, do not open the top cover.

3. Do not place anything inside

Do not place metal objects or spill liquid inside the MagCore® System. Electric shock

or malfunction may result.

Note On Use:

1. Read Instructions – All the safety and operating instructions should be read before the

product is operated.

2. Retain Instructions – The safety and operating instructions should be retained for future

reference.

3. Heed Warnings – All warnings on the product and in the operating instructions should be

adhered to.

4. Follow Instructions – All operating and use instructions should be followed.

5. Cleaning – Unplug this product from the wall outlet before cleaning. Only allow to use 75% of

EtOH to clean the surface of instrument.

6. Attachments – Do not use attachments not recommended by the product manufacturer as

they may cause hazards.

7. Water and Moisture – Do not use this product near water – for example, near a bath tub,

wash bowl, kitchen sink, or laundry tub; in a wet basement; or near a swimming pool; and the

like.

8. Accessories – Do not place this product on an unstable cart, stand, tripod, bracket, or

table. The product may fall, causing serious injury to a child or adult, and serious damage

to the product. Use only with a cart, stand, tripod, bracket, or table recommended by the

manufacturer, or sold with the product. Any mounting of the product should follow the

manufacturer’s instructions, and should use a mounting accessory recommended by the

manufacturer.

9. Ventilation – Slots and openings in the cabinet are provided for ventilation and to ensure

reliable operation of the product and to protect it from overheating, and these openings

must not be blocked or covered. The openings should never be blocked by placing the

product on a bed, sofa, rug, or other similar surface. This product should not be placed in a

built-in installation such as a bookcase or rack unless proper ventilation is provided or the

manufacturer’s instructions have been adhered to.

10. Power Sources – This product should be operated only from the type of power source

indicated on the marking label. If you are not sure of the type of power supply to your home,

consult your product dealer or local power company. For products intended to operate from

battery power, or other sources, refer to the operating instructions.

11. Grounding or Polarization – This product may be equipped with a polarized alternating-

current line plug (a plug having one blade wider than the other). This plug will fit into the

power outlet only one way. This is a safety feature. If you are unable to insert the plug fully

into the outlet, try reversing the plug. If the plug should still fail to fit, contact your electrician to

replace your obsolete outlet. Do not defeat the safety purpose of the polarized plug.

12. Power-Cord Protection – Power-supply cords should be routed so that they are not likely to

be walked on or pinched by items placed upon or against them, paying particular attention to

cords at plugs, convenience receptacles, and the point where they exit from the product.

13. Lightning – For added protection for this product during a lightning storm, or when it is left

unattended and unused for long periods of time, unplug it from the wall outlet and disconnect

the antenna or cable system. This will prevent damage to the product due to lightning and

power-line surges.

14. Overloading – Do not overload wall outlets, extension cords, or integral convenience

receptacles as this can result in a risk of fire or electric shock.

15. Object and Liquid Entry – Never push objects of any kind into this product through openings

as they may touch dangerous voltage points or short-out parts that could result in a fire or

electric shock. Never spill liquid of any kind on the product.

16. Servicing – Do not attempt to service this product yourself as opening or removing covers

may expose you to dangerous voltage or other hazards. Refer all servicing to qualified service

personnel.

17. Damage Requiring Service – Unplug this product from the wall outlet and refer servicing to

qualified service personnel under the following conditions:

a) When the power-supply cord or plug is damaged,

b) If liquid has been spilled, or objects have fallen into the product,

c) If the product has been exposed to rain or water,

d) If the product does not operate normally by following the operating instructions. Adjust

only those controls that are covered by the operating instructions as an improper adjustment

of other controls may result in damage and will often require extensive work by a qualified

technician to restore the product to its normal operation,

e) If the product has been dropped or damaged in any way, and

f) When the product exhibits a distinct change in performance

– this indicates a need for service.

18. Replacement Parts – When replacement parts are required, be sure the service technician

has used replacement parts specified by the manufacturer or have the same characteristics as

the original part. Unauthorized substitutions may result in fire, electric shock, or other hazards.

19. Safety Check – Upon completion of any service or repairs to this product, ask the service

technician to perform safety checks to determine that the product is in proper operating

condition.

20. Heat – The product should be situated away from heat sources such as radiators, heat

registers, stoves, or other products that produce heat.

The exclamation point within an equilateral triangle isintended

to alert the user to the presence of important operating

and maintenance (servicing) instructions in the literature

accompanying the appliance.

Be aware of contaimination by contagious specimens.

High temperature warning!!

DO NOT touch the heating block with this sticker attached, it may

cause serious burning injuries.

The lightning flash with arrowhead symbol, within an equilateral

triangle, is intended to alert the user to the presence of uninsulated

“dangerous voltage” within the product’s enclosure that may

be of sufficient magnitude to constitute a risk of electric shock to

persons.

SAFETY INSTRUCTIONS

2

RBCBioscience

Model Super / HF16 Plus

Processing Capacity Max .16 samples per batch

System Components 1. Pipetting Unit: Dispensing, transferring, X -Y two axis movements.

2. Electric Control: PLC module and Arm-based main board embedded in.

3. UV Light: Power 8W, life duration 1,000 Hrs .

4. D2 Lamp life: 800 hours. ( MagCore Super Only )

5. Heating Block: RT~90° C .

6. OD detection range: ABS <6. ( MagCore Super Only )

7. Detection source: D2 lamp. ( MagCore Super Only )

8. Detection wavelength: 260nm, 280nm,320nm.

9. Display Screen: 7 inch colored touch panel .

10. Accessories: T - Rack, Cartridge Rack .

11. Barcode Scanner(MagCore HF16 Plus optional), embedded thermal printer. (MagCore Super only)

Power Supply Voltage: AC 100V~ 240V; Frequency: 50/60Hz

Dimension W760 x D710 x H770 (mm) / W27.95 x D29.92 x H30.31 (inches)

Net Weight 76.5kg / 168.68LB (MagCore Super) / 68.5kg / 151LB (MagCore HF16 Plus)

Specification

━ Operating Parameters

━ Operating Environment

━ Applications

Applications: Applications: Whole Blood DNA (200µl/400µl/1,200µl), Viral Nucleic Acid (DNA/RNA 200µl/400µl/1,200µl), FFPE Genomic

DNA, Forensic DNA, Plasma and Circulating Cell-Free DNA (1,200µl), Tissue Genomic DNA, Plant Genomic DNA, Bacterial DNA, Whole

Blood total RNA, Cultured Cells total RNA.

Processing Capacity 1~16 samples per batch

Processing Time 30-90 minutes (depends on sample type and method)

Sample Volume 200/400/1200 μl

Elution Volume 60/100/150/200 μl

Purity DNA : O.D A260/280 ratio 1.8 ± 0.1

RNA : O.D A260/280 ratio 2.0 ± 0.2

Temperatures allowed

during transportation

storage

packaging

15°C~35°C

Temperatures allowed

during

operation

18°C~30°C

Pollution Degree Indoor

3

INDEX

Safety Precautions…………………………………………………………………………………………………… 1

Specification…………………………………………………………………………………………………… 2

Accessories…………………………………………………………………………………………………… 5

Installation…………………………………………………………………………………………………… 6

Before Install…………………………………………………………………………………………………… 6

IQ/OQ/PQ Documents…………………………………………………………………………………………………… 7

IQ Documents…………………………………………………………………………………………………… 7

OQ Documents…………………………………………………………………………………………………… 8

PQ Documents…………………………………………………………………………………………………… 9

System Overview…………………………………………………………………………………………………… 10

Modulen Position Description…………………………………………………………………………………………………… 11

Unpacking/Prepare Unpacking Tools…………………………………………………………………………………………………… 12

Install MagCore® HF16 Plus/Super ……………………………………………………………………………………………………13

Installation settings and test…………………………………………………………………………………………………… 14

Touch Panel and User Interface…………………………………………………………………………………………………… 15

Install MagCore® Cuvette…………………………………………………………………………………………………… 16

Install reagent cartridge…………………………………………………………………………………………………… 16

Install Tube, Tip…………………………………………………………………………………………………… 16

Emergent Stop…………………………………………………………………………………………………… 17

Start Programs…………………………………………………………………………………………………… 18

If the test result of the optic

measurement is over detection

(MagCore® Super Only)…………………………………………………………………………………………………… 19

Stop…………………………………………………………………………………………………… 21

Enable the barcode function

(MagCore® HF16 Plus Only)…………………………………………………………………………………………………… 21

Barcode scanning and editing

(MagCore HF16 Optional)…………………………………………………………………………………………………… 22

Optic data

(MagCore® Super Only)…………………………………………………………………………………………………… 22

Easy maintenance…………………………………………………………………………………………………… 23

Replace O-Ring…………………………………………………………………………………………………… 23

Piston Test…………………………………………………………………………………………………… 23

Clean Piercing Needles…………………………………………………………………………………………………… 25

Reject Tip…………………………………………………………………………………………………… 25

Heater Test…………………………………………………………………………………………………… 26

Optic Test…………………………………………………………………………………………………… 26

UV sterilization…………………………………………………………………………………………………… 27

Alarm History…………………………………………………………………………………………………… 27

System Status…………………………………………………………………………………………………… 28

Set up the system time…………………………………………………………………………………………………… 28

Update Program…………………………………………………………………………………………………… 29

Clean the Waste Box…………………………………………………………………………………………………… 29

Replace thermal paper…………………………………………………………………………………………………… 30

Replace fuse…………………………………………………………………………………………………… 30

Advanced maintenance…………………………………………………………………………………………………… 31

Disassemble the back service lid…………………………………………………………………………………………………… 33

Disassemble the top service lid…………………………………………………………………………………………………… 34

Axis directions…………………………………………………………………………………………………… 35

Replace the UV lamp…………………………………………………………………………………………………… 36

Replace LED Light…………………………………………………………………………………………………… 37

4

RBCBioscience

Replace the optic lamp

(MagCore® Super Only)…………………………………………………………………………………………………… 38

Replace the pistons…………………………………………………………………………………………………… 39

Replace the ejector rod module…………………………………………………………………………………………………… 41

Replace the heater module…………………………………………………………………………………………………… 42

Replace the gas spring on front door…………………………………………………………………………………………………… 43

Replace Door Sensor…………………………………………………………………………………………………… 44

Replace the M-axis sensors…………………………………………………………………………………………………… 45

Replace the sensors of

C, V, X and Y axes…………………………………………………………………………………………………… 46

Replace Cooling Fans…………………………………………………………………………………………………… 47

Replace the X-axis stepper motor…………………………………………………………………………………………………… 48

Replace the Y-axis motor…………………………………………………………………………………………………… 49

Replace the Y-axis belt…………………………………………………………………………………………………… 50

Replace the V-axis motor…………………………………………………………………………………………………… 51

Replace the C-axis motor…………………………………………………………………………………………………… 53

Replace the C-axis timing belt…………………………………………………………………………………………………… 54

Replace the lift motor of the base…………………………………………………………………………………………………… 55

Replace the timing belt of the lift

motor of the base…………………………………………………………………………………………………… 56

Replace PCB…………………………………………………………………………………………………… 57

Replace PLC…………………………………………………………………………………………………… 58

Replace Power Supply…………………………………………………………………………………………………… 59

Replace Touch Panel…………………………………………………………………………………………………… 60

Replace Printer…………………………………………………………………………………………………… 61

Electrical control and engineering

software…………………………………………………………………………………………………… 62

System…………………………………………………………………………………………………… 63

Parts…………………………………………………………………………………………………… 64

Main board configuration…………………………………………………………………………………………………… 65

Copy the engineering software to the

desktop…………………………………………………………………………………………………… 66

Install the HMI updating software…………………………………………………………………………………………………… 66

Create an uploading folder…………………………………………………………………………………………………… 67

Install the driver…………………………………………………………………………………………………… 68

HMI updating…………………………………………………………………………………………………… 70

PLC updating…………………………………………………………………………………………………… 71

Position Teaching Tools…………………………………………………………………………………………………… 74

The pre-operation before

calibration…………………………………………………………………………………………………… 75

X-axis calibration…………………………………………………………………………………………………… 76

Y-axis calibration…………………………………………………………………………………………………… 78

C-axis calibration

( Magcore® Super Only)…………………………………………………………………………………………………… 79

Automatic Optical Measurement…………………………………………………………………………………………………… 81

Optical Calibration Flow Chart…………………………………………………………………………………………………… 82

Empty Cuvette Test of standard

cuvette…………………………………………………………………………………………………… 82

Standard Cuvette Vaule…………………………………………………………………………………………………… 83

Adjust integration time…………………………………………………………………………………………………… 84

Test Standard Sample…………………………………………………………………………………………………… 85

Trouble Shooting for Alarm Code…………………………………………………………………………………………………… 86

5

Accessories

1. T-Rack X 2

2. Cartridge Rack X 2

3. Power Cable X 2 (Europe standard and U.S.A standard)

4. Grease X 1

5. 3mm O-Rings X 16

6. 5mm O-Rings X 16

7. Empty Cartridge X 10

8. Sample Tube /Elution Tube X 20

9. Pipette Tip X 20

10. MagCore Cuvette Set(10pcs) X 1

11. Waste Box X 1

12. Barcode Scanner X 1 (MagCore Super Only)

1 2 345

7

6

8 9

10 11 12

You can purchase Cat. No.: MSC100 MagCore Cuvette Set (X100) from the

local distributor.

(MagCore Super Only) (MagCore HF16 Optional)

6

RBCBioscience

770 mm

Left Side (Keep space clear) Height (Keep space clear)

Cooling Fans

710mm

760mm

Depth

Width

━ Before Install

Installation

Please refer to the machine size shown in the above figure to prepare an appropriate position for the

machine and reserve space for thermal dissipation.

7

━ IQ Documents

IQ/OQ/PQ Documents



MagCore Super and HF16 Plus

Installation Qualification/Operation Qualification/Performance

Qualification

SerialNo.____________________Date____________

InstallationQualification:

IQchecklist PASSFAILNOTE

MachineInspection:

*Noobviousdefectoncasing.

*Checkhandle.

*NoobviousdefectonPMMALOGOsurface.

*CheckT‐rack.

*CheckCartridgerack.

*Checkdoorfunction.



□



□



FunctionTest:

*Checkoutletvoltage:110~220V.

*Turnonmachine.

*Check7inchtouchpanel.

*CheckLEDLOGOlight.

*Barcodescanfunction(NotforHF16Plus).

*UVlampTest.

*MotorTest(AllAxisTest).

*RejectTipTest(Actsmoothly).

*HeaterTest(RTto60Clessthan7mins).

*SystemTimeSetup(InSystemStatus).



□



□



Examination

Signature

 Verification

Signature



Page1of3

8

RBCBioscience

━ OQ Documents



OperationQualification:

OQchecklist PASSFAILNOTE



ProgramCode999:

(SampleVolume:400μL,ElutionVolume:200μL,

OpticalMeasurement:Disable)

*Initialcheck.

*Perforatemotioncheck.

*Tiptakingmotioncheck.

*Pistonaspirationcheck.

*Magneticseparationmotioncheck.

*STOPfunctioncheck(AbortandRejecttip)



OpticTest:(NotforHF16Plus)

(EmptyCuvette,PlaceEmptyCuvetteinSlotNo.1)

*Thecountvaluein260nmshould>7000.



□



□



□



□



Examination

Signature

 Verification

Signature



Page2of3

9

━ PQ Documents



PerformanceQualification:

ThePerformanceQualificationshouldbedesignedaccordinguser’srequirement.

ThefollowingformatisanexampleofPerformanceQualification.



Sample KitSpec.ResultPASS/FAIL



400LWholeBlood



MagCore

GenomicDNA

WholeBloodKit

MGB400‐03

Cartridge102



Totalyield

>14g



Purity

OD260/280=

1.8~2.0



Examination

Signature

 Verification

Signature



Page3of3

10

RBCBioscience

System Overview

Front Door

Printer (MagCore® Super Only)

Touch Panel (HMI)

Top Service Lid

Back Service Lid

Cooling holes

Operation Area

Barcode Scanner

(HF16 Plus Optional)

Main Power Switch

and Power Outlet

Barcode

Scanner Port

USB Port

Slots

There are totally 16 slots

(number 1~16 from left to

right)

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

11

━ Module Position Description

Liquid handling

module

Work

platform

Heating module

Optic module

(Super Only)

Frame

Gantry structure

12

RBCBioscience

Unpacking

Prepare Unpacking Tools

If the cargo impacted, White Red If the cargo upside down, Sliver Red

2. Phillips Screwdriver

1. Hex Wrench (3mm)

3. Scissors

13

Install MagCore® HF16 Plus/Super

Proceed installation procedures after placing MagCore on a solid bench-top.

1.Use screw drivers to loose the screws as show in the picture.

2.Remove the red fixing plates.

3.Save all the materials for maintenance and shipment in the future.

Super Only

14

RBCBioscience

━ Installation settings and test

Step 1.

Please set up the local time.

Please refer to System Status chapter for the

information about the operation.

(Plase refer to P.28)

Step 2.

Test piston

Please refer to Piston Test chapter for the

information about the operation.

(Plase refer to P.23)

Step 3.

Test heater

Please refer to Heater Test chapter for the

information about the operation.

(Plase refer to P.26)

Step 4

Test optic module

(MagCore® Super Only)

Please refer to Optic Test chapter for the

information about the operation.

When the optic module test is finished, please

compare the result with the factory document to

make sure the function of optic module.

(Plase refer to P.82)

SystemTime Setup

Month

Day

Minute

Current Time

Year Hour

59

01

2014 23

2014/01/01 23:59

CancelUpdate

15

Touch Panel and User Interface

MagCore® Super / HF16 Plus provides 7-inch

full-color touch panel and easy-to-use user

interface. Operator only needs to pay attention

to the panel to know the current status, ending

time and test report of the machine. The above

figure shows the function list of the operation

screen and the description of the status icons.

Start Maintenance

Barcode Optics Data

Start Maintenance

Barcode Oprics Data

Start

Barcode

Maintenance

Optics

Execute the

extraction process Easy maintenance

and system setting

functions

Optics data browsing and

output functions

(MagCore Super only)

Barcode scanning,

editing, printing

functions

(MagCore HF16 Plus

optional)

16

RBCBioscience

━ Install Tube, Tip

Warning :

Please do not use the Tips and Tubes which are not provided by the original manufacturer.

The test result may be not correct and the machine may be damaged due to different Tips

and Tubes.

━ Install reagent cartridge━ Install MagCore® Cuvette

(MagCore Super Only / for optic test)

1. Please put MagCore® Cuvette into the

corresponding sample's well. The installation

of Cuvette is directional. It is impossible to put

it into the well if the direction is not correct.

2. Put the 200 μl SP tip into the W4 of the T-Rack.

Please insert the front end of the cartridge into the space

below the fixing plate of the Cartridge Rack.

1. Put the tip into the corresponding well according to the left

figure.

2. Put the T-Rack on the machine.

Please install the Tip and Tube

according to the instructions of

extraction kit user manual.

Please insert the Cartridge Rack before

the T-Rack.

W5 Elution Tube

W4 200μl SP Tip

W3 Pipette Tip

W2 DNase / Tip Holder set

W1 Sample Tube - FS Tip

When installing it,

please do not scratch

the optic test window.

17

Stop

Program Processing

201

Program Code :

Start

Remaining Time

Process Message2014/01/01 23:59:59

10 / 75

D2 Lamp OFF

━ Emergency Stop

Please read the security information to correctly stop the machine

when an accident takes place.

When an emergency situation occurs and the

machine is running, please press the stop button or

open the door of the machine to stop the machine

immediately.

18

RBCBioscience

Please confirm the number of the reagent set

which you install.

Select the number of the cartridge.

Select DNase Treatment. (Only for RNA kit.)

Select Sample Volume.

Select Elution Buffer. (Only for tissue kit.)

Please pretreat the sample according to the

instructions of the user manual of the MagCore®

Kit and put consumables into the machine.

Press Start to go to the next step.

System will ask you whether or not to scan the

barcode.

If there is a barcode record from previous test

processes, the system will ask you whether or

not to delete previous barcode record. If there is

no barcode record, the system will not ask you.

(MagCore Super only; HF16 Plus optional)

Start Maintenance

Barcode Optics Data

Select Cartridge Code

Cancel

101

102 202

201

Prev Next

610

Program Code :

DNase Treatment

Ye

sN

o

Back

201

Program Code :

Select Sample Volume

200 400

Back

401

Program Code :

Select Elution Buffer

Water Tris

Back

Start Barcode Scanning?

Yes

Barcode

No

Delete All Barcode Record ?

Yes

Barcode

No

━ Start Programs

!The number can be

found in the sticker on

the cartridge.

1

2

3

5

6

4

19

━If the test result of the optic

measurement is over detection

Select to enable or disable the optic measurement

function. (MagCore Super Only)

( 201, 202, 211, 105 without the function)

Please confirm the parameters and press the Start

button to execute the program.

Automatic extraction process.

The extraction process is completed.

If you select the optic measurement funtion, you

can browse, print or output the test result to a USB

Flash Drive (please refer to the optic data chapter).

If the test result of the optic test shows the

concentration is over detection ( DNA >300ng/μl /

RNA > 240 ng/μl), the system will ask you to dilute.

A.Please record and save the

current measured data.

Please select YES.

Please check the value of each sample. If

there is a value showing DNA >300 ng/

μl or RNA > 240 ng/μl, please record

the number of the sample. It means the

sample needs to be diluted. After recording

the number of the slot, please insert the

USB Flash Drive and press the Save button

to save to current measured data. After

finishing the above steps, please press Next

Step.

10

7

8

9

102

Program Code :

Select Optic Measurement Function

Enable Disable

Back

Press Start to Process

Back

201

Program Code :

Start

N/A

200

N/A

300

Enable

DNase :

Sample Volume :

Elution Buffer :

Elution Volume :

Optical Measurement :

Program Completed

OK

Completed

Print Data Review Data OK

Program Completed

OK

Program Completed

10X Dilution ?

Print Data YesNo

Program Completed

OK

Program Completed

10X Dilution ?

Print Data YesNo

Note the samples which need dilution

1234567890

> 300

2.05

2.364

1.154

ng / μL ( DNA )

260 / 280

A260

A280

2014 / 01 / 30 09:19:59

Date

NextNext StepSave

12345678910 11 12 13 14 15 16

Stop

Program Processing

201

Program Code :

Start

Remaining Time

Process Message2014/01/01 23:59:59

10 / 75

D2 Lamp OFF

1

The values are DNA >300 ng/μl /

RNA > 240 ng/μl

Please save the data in the USB Flash Drive

and transfer to computer, or the data in

the USB Flash Drive may be overwritten

later.

File name: OpticsSampleData.cvs

(MagCore Super Only)

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RBCBioscience MagCore Super Training manual

RBCBioscience MagCore Super Training manual

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