Omega PHB-215, PHB-220, PHB-225, PHB-250 Owner's manual

Brand: Omega

Model: PHB-215, PHB-220, PHB-225, PHB-250

Category: Measuring, testing & control

Type: Owner's manual

http://www.omega.com

e-mail: [email protected]

User’s Guide

PHB-215 pH Meter

PHB-220 pH/Conductivity Meter

PHB-225 pH/Ion Meter

PHB-250 pH/Ion/Conductivity Meter

PHB-215

pH m

eter

Servicing North America:

USA: One Omega Drive, Box 4047

ISO 9001 Certified Stamford, CT 06907-0047

Tel: (203) 359-1660 FAX: (203) 359-7700

e-mail: [email protected]

Canada: 976 Bergar

Laval (Quebec) H7L 5A1

Tel: (514) 856-6928 FAX: (514) 856-6886

e-mail: [email protected]

For immediate technical or application assistance:

USA and Canada: Sales Service: 1-800-826-6342 / 1-800-TC-OMEGA

Customer Service: 1-800-622-2378 / 1-800-622-BEST

Engineering Service: 1-800-872-9436 / 1-800-USA-WHEN

TELEX: 996404 EASYLINK: 62968934 CABLE: OMEGA

Mexico and

Latin America:

Tel: (95) 800-TC-OMEGA

FAX: (95) 203-359-7807

En Espan÷ol: (203) 359-7803 e-mail: [email protected]

Servicing Europe:

Benelux: Postbus 8034, 1180 LA Amstelveen, The Netherlands

Tel: (31) 20 6418405 FAX: (31) 20 6434643

Toll Free in Benelux: 06 0993344

e-mail: [email protected]

Czech Republic: ul. Rude armady 1868, 733 01 Kavrine-Hranice,

Czech Republic

Tel: 420 (69) 6311627 FAX: 420 (69) 6311114

e-mail: [email protected]

France: 9, rue Denis Papin, 78190 Trappes

Tel: (33) 130-621-400 FAX: (33) 130-699-120

Toll Free in France: 0800-4-06342

e-mail: [email protected]

Germany/Austria: Daimlerstrasse 26, D-75392 Deckenpfronn, Germany

Tel: 49 (07056) 3017 FAX: 49 (07056) 8540

Toll Free in Germany: 0130 11 21 66

e-mail: [email protected]

United Kingdom: 25 Swannington Road, P.O. Box 7, Omega Drive,

ISO 9002 Certified Broughton Astley, Leicestershire, Irlam, Manchester,

LE9 6TU, England M44 5EX, England

Tel: 44 (1455) 285520 Tel: 44 (161) 777-6611

FAX: 44 (1455) 283912 FAX: 44 (161) 777-6622

Toll Free in England: 0800-488-488

e-mail: [email protected]

omega.comomega.com

OMEGA

OMEGAnet

On-Line Service Internet e-mail

http://www.omega.com [email protected]

It is the policy of OMEGA to comply with all worldwide safety and EMC/EMI regulations that

apply. OMEGA is constantly pursuing certification of its products to the European New Approach

Directives. OMEGA will add the CE mark to every appropriate device upon certification.

The information contained in this document is believed to be correct but OMEGA Engineering, Inc. accepts

no liability for any errors it contains, and reserves the right to alter specifications without notice.

WARNING: These products are not designed for use in, and should not be used for, patient connected applications.

Quick Start Guide for pH Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . Page ii

Introduction

Menu Keys,Softkeys and Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Electrode Connectors and Inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

LCD Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Function Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Electrodes

Preparing pH and Ion Selective Electrodes. . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Connecting Electrodes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Using and Storing Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

pH Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Solid-State FET Electrodes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Ion Selective Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Meter Setup

Meter Setup Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

pH Mode

pH Mode Standardization Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Cal Reminder Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Select Custom Buffer Set. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

pH Mode Options Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Standardizing and Measuring pH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Clearing Buffers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

mV Mode

mV Mode Standardization Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

mV Mode Options Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Clearing Relative mV Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Quick Start Guide for Ion Measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Ion Mode

Ion Mode Standardization Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Standardizing and Measuring Ion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Measuring Ion using a Known Addition type (incremental ion) method. . . 17

Quick Start Guide for Conductivity/Resistivity/Salinity/TDS Measurement. . . . 18

Conductivity/Resistivity/Salinity/TDS Modes

Conductivity/Resistivity/Salinity/TDS Standardize Menu . . . . . . . . . . . . . . . . 19

Conductivity/Resistivity/Salinity/TDS Options Menu. . . . . . . . . . . . . . . . . . . . 20

Data logging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Meter Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

pH Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Ion Selective Electrode Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Determining Isopotential Point . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

RS-232 Serial Interface Meter Command Set. . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Table of Contents

1.Connect power cable to meter connector on

the rear panel marked “power”and to AC power

source.

2.Connect the glass pH/ATC electrode to the

channel A BNC connector marked “ch.A”and to

the channel A temperature connector marked

“temp A”.

3.Verify the meter is in pH mode on channel A.Use

the Mode key and Channel key to set the meter

to the correct mode and channel if necessary.

(See Function keys).

4.Standardize the electrode by immersing the

electrode in a buffer,pressing Standardize, press-

ing 1) Auto-enter a buffer and following the

prompts.Repeat this step to enter each buffer.

The meter will check the electrode and buffers,

and give an error message if there is a problem.

Press Standardize to enter or clear buffers,select

buffers, set resolution,or set other parameters for

the current mode and channel.

5.The display shows the current measurement,and

indicates a stable reading with the indicator.

Press Cal Data to review and graph the elec-

trode calibration data.

Quick Start Guide for pH Measurement

. A

f. A

. A

. B

. C

. A

f. A

. A

. B

. C

A 7.000

25.0

No buffers.

Mode

Channel A: pH mode

Standardize Menu

1) Auto-enter a buffer

2) Manual buffer entry

3) Clear buffers

4) Options menu

5) Cal reminder menu

6) Select buffer set

channel

indicator

units

indicator

Channel

Standardize

A 7.000

25.0

4.008 pH

7.000

entered

buffers

“power”

“ch. A”

“temp A”

stability

indicator

This Omega meter is a powerful,versatile and accurate instrument.It features

easy menu-based operation with easy to understand prompts and

electrode/standard error checking.

These meters feature many advanced options, such as programmable stability

criteria,programmable standardization reading delay times,multi-channel oper-

ation,fast reading update rates of twice per second for all channels,program-

mable alarms,programmable data logging of 500 data points and a superb RS-

232 serial interface for controlling the meter and obtaining data.

Omega meters use flash programmable ROM for the operating code.The meter

operating code can easily be upgraded as new features are made available.

Direct Menu Keys, Softkeys and Display

The meter uses six Direct Menu keys to access the menus and operations (such

as selecting pH mode,standardizing,checking electrode calibration data,

selecting the electrode channel).

There are four Softkeys that provide additional operations; these Softkeys

change their function as needed and each Softkey has an icon to indicate its

current function.

The display is a backlit quarter-VGA screen capable of displaying all four elec-

trode channels (of a PHB-250) simultaneously. The backlight will turn off after a 45

minute period of non-use; pressing any key will automatically turn the backlight

on again.

A S

7.001

25.0¡C

4.008 pH

7.000

6/30/1998 02:28 PM

Denver Instrument

Introduction

Softkeys

Direct Menu

Keys

Direct Menu

Keys

Numeric

Keypad

PHB-215

pH m

eter

Electrode Connectors and Inputs

BNC ("ch.A" or "ch.B") connectors: pH,Ion

Selective Electrodes and ORP (redox) elec-

trodes attach to the meter through a BNC

("round twist-on") connector to channel A or

channel B (PHB-225 and PHB-250 only).

Temperature ("temp A" or "temp B") connec-

tors: use to connect the 2.5mm mini-phone

plug from the temperature sensor (built into

the pH electrode with the Omega standard

pH/ATC electrode) for Automatic Temperature

Compensation (ATC).

Reference ("ref.A" or "ref.B") connectors: use

for attaching a reference electrode tip-pin

plug when a separate reference electrode is

used.

Conductivity DIN (“ch.C conductivity”)

connector: use to connect a 4-band conduc-

tivity/ATC cell.These 4-band cells offer

improved linearity and stability over older 2-

band conductivity cells. (PHB-220 and PHB-250

only).

FET DIN ("ch.F FET") connector: use to connect

the Denver Solid-State Field Effect Transistor

(FET) pH/ATC electrode. These non-glass pH

electrodes offer certain advantages over con-

ventional glass pH electrodes.

Serial port ("RS232") DB-9 connector: use to

connect a serial printer or Personal Computer.

This bi-directional interface outputs data and

receives meter commands.

Power (“power”) connector: use to connect a

5.5mm OD x 2.1 ID coaxial connector with

12VDC at 500mA (center pin negative).

Introduction

ch. A

ref. A

temp

RS232C

power

ch. F

FET

ref

Connectors for PHB-250

Note: Not all of the following will display at the

same time.

. Result: current measurement.

. Units: displays the units for the current measure-

ment.Examples: pH,mV, mg/L F-,µS/cm or

W-cm.

. Softkey icons: show the current function

assigned to each softkey.

. Calibration due reminder: the icon means

a calibration is now due.

. Date and time: displayable in different formats.

. Datalogging: the icon indicates datalog-

ging is active.

. Buffers/Standards: in single channel mode,all

entered buffers or standards are displayed.A

“!”symbol beside a buffer indicates that buffer

is out of the entered calibration valid time (See

Calibration reminder,page 9).

. Temperature: displays the measured tempera-

ture when an electrode with ATC or separate

temperature probe is attached.Shows “M”

when a manually entered temperature is being

used.

. Alarm: “

”indicator means data is outside the

set alarm limits.

Channel: indicates which electrode channel

(input) is being displayed.Channel A and

Channel B (PHB-225 only) are BNC inputs.

Channel F is the FET electrode input.

. Stability: the indicates the electrode is sta-

ble to the selected criteria.

. Multiple Channel: display can show two

(PHB- 215),three (PHB-220 and PHB-225) or four

(PHB-250) electrode measurements with tem-

perature simultaneously.

. Out-of-range or non-valid reading: dashes indi-

cate a measurement is not available.This usual-

ly means the reading is out of range,or can

mean in ion mode that no standards have

been entered,or strict calibration has been set

and the calibration expired.

Display

A 7.000

25.0

4.008 pH

7.000

9.999

6/30/1998 02:28 PM

A B

Single channel display

A 6.998

24.6

B 1.02

mg/L

23.4

C F-

30-6-1998 14:28:05

Dual channel display

A pH

23.8

1998.6.30 14:28:30

CAL!

Log

. Mode: Selects the mode: pH,mV

(PHB-215),Ion (PHB-225 and PHB-250),

Conductivity - Resistivity-NaCl Salinity-

Practical Salinity-TDS (PHB-220 and

PHB-250) to use for the currently selected

channel (electrode input).

. Standardize: Enters buffers or standards

for the currently selected channel and

mode.Use to enter pH buffers, relative mV

offset,ion standards or conductivity /resis-

tivity standards.

Also used to change other settings which

affect the measurement.

. Cal Data: Displays and graphs buffers or

standards with time and date stamp and

electrode calibration data for the select-

ed channel and mode.

. Channel: Selects the channel(s) (elec-

trode inputs) to display.The PHB-215 can

display one or two channels simultane-

ously (Channels A and F).The PHB-225

can display one,two or three channels

simultaneously (Channels A, B and F).The

PHB-220 can display up to three channels

(Channels A,C and F).The PHB-250 can

display up to four channels (Channels A,

B,C and F).

. Setup: The Setup menu is used to set vari-

ous general meter settings,such as date

and time,display contrast,keypress beep

and serial port.

. Data Log: Displays the datalogging menu

used to set datalogging and view the

stored Data Log (see Datalogging).

. Clear: Exits from the current menu and

returns to the previous menu,cancels the

current operation or clears a number

entry.

. Enter/Print: Accepts numeric values,menu

selections or pending operations.In the

main measure screen,acts as a Print key,

sending all current measurements to a

printer/ computer through the serial port

and stores the measurements in the Data

Log.

. Softkeys: These four keys access different

operations at different times.Most menus

offer a “Help”softkey and the “Measure”

softkey, which allows a direct return to the

main measuring screen,exiting all menus

immediately. The “Up Arrow”and “Down

Arrow” softkeys offer one way to select a

menu item.The “Left Arrow”key is a back-

space,active during number entry.

. Numeric Keys: Pressing a number key

selects a numbered item in a menu.The

number keys also allow entering values for

buffers, standards,and various meter set-

tings.

Function Keys

–

Help

Measure

Scroll up

Scroll down

Backspace

Graph

Exponent number entry

Incremental ion method

Measure lock

Measure unlock

Softkeys

Function Keys

Mode

Standardize

Cal Data

Channel

Enter/Print

Clear

Setup

Data Log

Channels

The Channel key is used to turn on or off each

available channel.In single-channel operation,

additional information for the selected channel is

provided,including a display list of all entered

buffers or standards.In multi-channel operation,

the Mode,Standardize and Cal Data menus ask

for the channel before accessing the menu.

The PHB-215 offers single or dual channel opera-

tion using channel A ( pH and ORP electrodes)

and channel F (Solid-state FET pH electrode).The

PHB-225 offers up to three-channel simultaneous

operation of channel A and channel B (pH,ORP

and Ion Selective Electrodes) and channel F

(Solid-state FET pH electrode).

The PHB-220 has Channel A (pH and ORP elec-

trodes),Channel C (Conductivity cells) and

Channel F (solid-state FET pH electrode).The

PHB-250 provides up to four channel measure-

ment with Channel A and Channel B (pH and

ORP and Ion Selective electrodes),Channel C

(Conductivity cells) and Channel F (solid-state

FET pH electrode).

Configure Display

1) Turn channel A on/off: ON

2) Turn channel B on/off: ON

3) Turn channel F on/off: OFF

4) Turn channel C on/off: ON

5) Measurement screen

PHB-250 Select Channel

screen

Channel

The meter allows you to use a variety of glass mem-

brane (“glass”) pH/ATC electrodes,ion selective elec-

trodes,Conductivity/ATC cells,the Omega Field Effect

Transistor (FET) Solid-State pH/ATC electrode,tempera-

ture (ATC) probes,combination electrodes using a BNC

connector, or separate electrode pairs with BNC con-

nector and reference pin.

Preparing Electrodes and Conductivity Cells

Remove the wetting cap or storage cap from the elec-

trode.Before first using your pH electrode or whenever

the electrode is dry,soak it several hours in an elec-

trode filling or storage solution (3 Molar KCl solution) or

in a buffer for pH electrodes.Condition ISE’s in the rec-

ommended solutions. Rinse Conductivity cells with

deionized water before use.

Connecting Electrodes

pH, ORP or ISE electrodes (with BNC connector):

Connect the electrode to the BNC input,either chan-

nel A or channel B (PHB-225 and PHB-250 only),located

at the rear of the meter.Push in and rotate the elec-

trode’s BNC connector until it locks in place.Connect

the ATC connector to the temp.A or temp.B connec-

tor. To disconnect,twist the BNC connector in the

opposite direction and pull.

Electrode Pair Using a Reference Electrode (with

Reference Pin Plug):

Connect the indicating electrode to the BNC input.

Connect the reference electrode to the Reference

input.Push the electrode’s tip pin plug into the input to

connect and pull out to disconnect.

Conductivity Cells (with DIN connector):

Align and push in the DIN connector fully to the chan-

nel C input (PHB-220 and PHB-250 only).Pull carefully to

disconnect.

Electrodes

To measure Use channel (connector)

pH A (BNC)*

B (BNC)*

mV (ORP) A (BNC)*

B (BNC)*

ion (ISE) A (BNC)*

B (BNC)*

Conductivity C (DIN)

Resistivity C (DIN)

Salinity C (DIN)

TDS C (DIN)

pH (FET) F (mini-DIN)

*Separate reference electrodes can be used with “Ref A” or

“Ref B”connectors

ch. A

ref. A

temp

ref

ch. F

FET

BNC

Connector

Channel C

Conductivity Input

ATC

Connector

Channel F

FET pH Input

Using and Storing Electrodes

pH Electrodes

• Provide moderate stirring for faster electrode

response.

• Leave the fill hole open during all use.

• Rinse the electrode between each measurement

with a portion of the next sample or buffer to be

measured,or with deionized or distilled water.

• Keep glass electrodes wet when not being used

by placing some electrode filling solution in the

wetting cap and storing with the wetting cap on.

• Keeping glass electrodes “wet”will improve their

performance.Store electrodes in electrode filling

solution or storage solution (3M KCl).

Solid-State FET Electrode

• All models allow use of both standard glass

pH/ATC and Solid-State FET (Field Effect Transistor)

pH/ATC electrodes.The meter can store a cali-

bration for both types of electrodes.Plug the FET

electrode into the channel F mini-DIN input.

• Allow the FET about 1 minute to stabilize when

first connected.The FET electrode can be stored

dry or in electrode storage solution. Provide mod-

erate stirring.

Ion Selective Electrodes

• Add proper amount of Ionic Strength Adjuster

(ISA) to all standards and samples,usually 1 mL

ISA to 50 mL standard or sample.

• Provide moderate stirring for faster electrode

response.

• Rinse the electrode(s) between each measure-

ment with a portion of the next sample or stan-

dard to be measured,or with deionized or dis-

tilled water.

• Follow the instruction sheets for the individual

electrode.Store as recommended.

Conductivity Cells

• When changing samples or standards,immerse

the cell into the new solution,then lift and allow

solution to drain out.Repeat two more times.

• Gently tap cell to dislodge air bubbles.

Electrodes

Store with wetting

cap and Fill

Solution (3M KCl)

Provide

moderate

stirring

Store FET and

Conductivity

Cells dry

Meter Setup Menu

Press Setup to access the Meter Setup menu:

1.Time and date menu: use to set the time format

(HH:MM AM/PM or HH:MM:SS),set the time, set the

date format (MM/DD/YY,DD-MM-YY or YYYY.MM.DD),

and set the date.

2.Select temperature units: use to select temperature

measurement and display in degrees Celsius,degrees

Fahrenheit or Kelvin.

3.Select contrast: use to select the display contrast,mak-

ing the displayed characters lighter or darker.Select

setting “5”for typical conditions.

4.Select baud rate: use to set the serial RS232 port baud

rate. This must match the baud rate setting of the

printer or computer being used with the meter.

5.Setup serial port: use to configure the serial port start

bits and parity setting. This must match the settings of

the printer or computer being used with the meter.

6.Keypress beep on/off: use to turn on or off a "beep"

upon each keypress as an audible signal that a key

has been pressed.

7.Select video color scheme: use to set the display to

black characters on a white background or white

characters on a black background.

8.Show meter information: use to show the meter

model,software version and serial number.

9.Enable measure lock: use to enable the measure lock

where a stable measurement is locked (frozen) for

later review. Stability criteria should be set to SLOW for

all channels and modes in use.

0.Enable strict calibration: use to set strict calibration

where no measurements are displayed if the calibra-

tion reminder has expired.

“±”Set screen saver timeout: use to set a time for the

backlight to turn off and the screensaver to activate.

"•" Restore factory defaults: use to reset all settings to

factory defaults.On occasion it may be useful to

completely reset the meter,for example,if other users

have changed a setting.

Warning!

A reset also clears all electrode standardizations.

Meter Setup

Meter Setup Menu

1) Time and date menu

2) Select temperature units

3) Select contrast

4) Select baud rate

5) Setup serial port

6) Keypress beep on/off

7) Select video color scheme

8) Show meter information

9) Enable measure lock

0) Enable strict calibration

±) Set screen saver timeout

•) Restore factory defaults

Setup

Select Time Format

1) hr : min : sec

2) hr : min AM/PM

Select Date Format

1) mm / dd / yyyy

2) dd – mm – yyyy

3) yyyy.mm.dd

Enable Measure Lock

Display lock/unlock softkey?

1) Yes

2) No

If “measure lock” is enabled, a

“lock” soft key will appear on

the measurement screen

Enable Strict Calibration

Require calibration?

1) Yes

2) No

If strict calibration is enabled,

no measurements will be

displayed for a channel with an

expired cal reminder.

pH Mode

pH Mode Standardize Menu

Press Mode and select 1) pH.

Press Standardize and the pH Mode Standardize

Menu appears:

1.Auto-enter a buffer: use to add a new buffer which

is auto recognized by the meter,or update an exist-

ing buffer.Follow the prompts.

2.Manual buffer entry: use to enter a buffer value by

manually entering the pH of the buffer.

3.Clear buffers: use to clear all buffers entered for the

current channel (pH mode).If all entered buffers are

being re-entered,it is usually not necessary to clear

buffers before re-entering them.

4.Options Menu: A menu of additional specific pH

mode settings. (See page 10).

5.Cal reminder menu: use to set a timer reminding

you to recalibrate.A icon will appear on the

main screen and an exclamation mark will appear

beside the buffers for which time has expired.

The calibration reminder is a reminder of when

electrode calibration (with buffers) should be

redone.It is based on elapsed time from the oldest

entered buffer.

If strict calibration is set (see Meter Setup Menu),when

a calibration has expired the icon appears,and

"- - - " is displayed in place of the measurement. No

measurements can be obtained until a calibration is

performed.

Note: When strict calibration is set,the calibration

reminders for all channels are turned ON, and can't

be turned off from the Cal Reminder Menu.

6.Select buffer set: There are five auto-recognition

buffer sets and the option to configure and use a

custom buffer set of your own.

Select custom buffer set

Use Custom Buffer Set to make a set of buffers con-

taining the specific buffers in use (up to five buffers).

Select Custom Buffer Set,configure the custom

buffer set,then buffers from this set will be automati-

cally recognized and entered.

Custom buffers can have any numeric pH value,or

can be selected from the built-in temperature cor-

rected buffers. Using the built-in buffers allow tem-

perature correction of the pH values of the buffers,

offering more accuracy.

Channel A: pH mode

Standardize Menu

1) Auto-enter a buffer

2) Manual buffer entry

3) Clear Buffers

4) Options Menu

5) Cal reminder menu

6) Select buffer set

Standardize

Channel A: pH mode

Manual Buffer Entry

Enter the actual pH of this

buffer, after correcting

for temperature:

Press Enter to accept.

Channel A: pH mode

Cal Reminder Menu

1) Turn reminder On/Off: OFF

2) Set reminder interval:

01, 00 : 00 days, hr : min

3) Standardization menu

When the cal reminder expires, a “CAL!”

(or “!”) icon will be displayed with the

measurement and with each expired stan-

dard. If strict cal is ON, reminders are

always ON, and expired channels show no

measurements.

Channel A: pH mode

Select Buffer Set

1) 2, 4, 7, 10, 12 at 25C

2) 2, 4, 7, 10, 12 at 20C

3) 1, 3, 6, 8, 10, 13

4) 1.68, 4.01, 6.86, 9.18, 12.46

5) 1.09, 3.06, 4.65, 9.23, 12.75

6) Select custom buffer set

Custom Buffer

No buffers

CAL!

Channel A: pH mode

Configure Custom Buffer Set

1) Add a “built-in” buffer

2) Add a manual buffer

3) Delete a buffer

4) Standardize menu

Custom Buffer Set

No buffers

CAL!

pH Mode Options Menu

1.Select resolution: use to set pH readings to 0.1,0.01,

or 0.001 pH units.

2.Select stability criteria: use to set stability criteria to

slow,medium or fast to match the electrode's speed

of response and the variability of the signal allowed

for a “stable”( ) measurement.

3.Select signal averaging: use to set filtering of the

electrode signal to very slow (10 readings),slow (8),

medium (6),fast (4) or very fast (2). Slower settings

give more stable readings,although may require

longer times to reach stability.

4.Set standardization delay: use to set a reading delay

time for the meter to wait before accepting an elec-

trode signal during standardization.Programming a

standardization reading delay helps slow responding

electrodes reach equilibrium before the electrode

signal is accepted.

5.Set pH slope: use to set a known electrode slope

used by the meter with a zero- or single-point stan-

dardization.The normal default slope is 59.16 mV/pH.

The meter allows between 80 and 120 % efficiency to

be entered.

6.Standardize menu: Returns to the pH mode

Standardization Menu.

7.Manual temperature menu: use to set a temperature

to be used in the absence of an ATC probe or when

manually overriding the ATC.

8.Data alarm menu: use to set pH limits.If the limits are

exceeded an alarm indication (“

”) is displayed and

recorded with any data points placed in the Data

Log.

9.Set isopotential point: use to set an isopotential point

for use in high accuracy electrode measurements

(See Isopotential,page 20).

pH Mode

Channel A: pH mode

Options Menu

1) Select resolution

2) Select stability criteria

3) Select signal averaging

4) Set standardization delay

5) Set pH slope

6) Standardize menu

7) Manual temperature menu

8) Data alarm menu

9) Set isopotential point

Notes:

1.Auto-recognized buffers are found in the Auto-rec-

ognized built-in buffer sets.These buffers are auto-

recognized by the meter, and are also automatically

temperature corrected for the variation of buffer pH

with temperature.

2.When manually entering buffers,the exact pH of the

buffer at the current temperature must be entered.

All buffers change pH with temperature.For best

accuracy,either use the built-in buffers or make sure

manually entered buffers are at the expected tem-

perature (so that their pH as entered is correct).

3.Auto-recognition Buffer Sets:

1) 2,4,7,10, 12 (nominal value adjusted at 25°C)

2) 2,4,7,10, 12 (nominal value adjusted at 20°C)

3) 1,3,6,8, 10,13

4) 1.68,4.01,6.86,9.18, 12.46 (NIST buffers)

5) 1.09,3.06,4.65,9.23, 12.75 (DIN buffers)

6) Select custom buffer set

4.Temperature Correction of Electrodes and Buffers

The meter automatically compensates for the temper-

ature dependence of the electrode's response when

measuring pH.The meter also compensates for buffer

change in pH value with temperature.Temperature

compensation is based on temperature either from an

ATC probe or a manually entered temperature.

Using a Solid-state FET (Field Effect Transistor)

pH/ATC Electrode

By turning channel F (FET) on,an Omega FET pH/ATC

electrode can be directly used.pH and mV modes are

available with the FET electrode.FET devices can have

large offset potentials that vary with each transistor

chip,so Manual buffer entry must be used to enter the

first buffer.After one buffer has been entered using

“Manual buffer entry”, following buffers can usually be

entered with “Auto-enter a buffer”.

pH Mode

Actual Buffer pH vs. Temperature

pH 4.00(4.01)/7.00/10.00 buffer (nominal 25°C)

Temperature Buffer 4 Buffer 7 Buffer 10

(°C)

30 4.016 6.991 9.947

25 4.008 7.003 10.000

20 4.003 7.020 10.057

15 4.000 7.042 10.119

10 3.998 7.069 10.187

Standardizing and Measuring pH

1.Immerse the electrode in a buffer and stir moderate-

ly.The meter displays the current pH measurement.

2.Allow the electrode sufficient time to reach

equilibrium.

3.Press Standardize,then press either 1) Auto-enter a

buffer or 2) Manual buffer entry.

4.Follow the prompts on the display.

5.The meter waits for a stable signal,automatically rec-

ognizes the buffer (if using “Auto-enter”),checks the

electrode and buffer and enters the buffer.The

entered buffer appears in the display.

6.Alternatively,if the signal is not stable,you can press

Enter when the reading stabilizes according to your

tolerance criteria.The meter then enters the buffer.

7.Repeat steps 1 through 4 to enter a second,third,

fourth or fifth buffer.With more than one buffer the

meter performs a diagnostic check on the electrode.

The electrode is considered good if the slope is

between 90 to 105%.If a sixth buffer is entered,the

buffer farthest away is replaced by the new buffer.

Hints to achieve better accuracy:

• During standardization,allow time for the electrode

to stabilize before entering the buffer into the meter.

• Standardize using at least two buffers,bracketing the

expected pH of your samples.

• Standardize at least daily for the most accurate read-

ings.

• Open the Fill Hole on the electrode.

• Stir all buffers and samples.

• Rinse the electrode with DI water between samples

and buffers.

• Always use fresh buffers.

Clearing Buffers

Press Standardize,then press 3) Clear buffers to clear

buffers. If all previously entered buffers will be re-

entered,it is not necessary to clear buffers since the

meter will replace the previous values.If re-entering

only some buffers, all the old buffers should be cleared.

Channel A: pH mode

pH Checklist

Prepare buffer or sample.

Rinse electrode.

Place electrode in solution.

Stir moderately.

Press Enter to continue.

Channel A: pH mode

Standardize Menu

1) Auto-enter a buffer

2) Manual buffer entry

3) Clear buffers

4) Options menu

5) Cal reminder menu

6) Select buffer set

Standardize

Channel A: pH mode

Manual Buffer Entry

Enter the actual pH of this

buffer, after correcting

for temperature:

Press Enter to accept.

4 7 10

pH buffers

samples

Electrode Potential,mV

Titrant Volume,mL

Redox Titration

Standardize

Millivolt measurements are used to measure ORP (oxi-

dation-reduction potential) or redox potential,to

check performance of pH or Ion Selective Electrodes,

and for redox titrations.

The meter will measure millivolts (mV) by pressing Mode

and selecting 2) mV.Relative mV can be measured

by entering a mV offset or using the current mV value

as the mV offset.

mV Standardization Menu

In mV mode,press Standardize and the mV mode

Standardization Menu appears:

1.Auto-enter mV offset: use to set the relative mV off-

set equal to the current mV reading. The current mV

becomes 0.0 relative mV.

2.Set mV offset: use to manually enter a mV offset.

3.Clear mV offset: use to clear any offset that has

been entered,returning the meter to absolute mV

mode.

4.Options menu: a menu of additional settings specif-

ic to the mV mode.See below.

mV Mode Options Menu

1.Select resolution: use to set mV readings to 1 or 0.1

millivolt resolution.

2.Select stability criteria: use to select stability criteria

for slow,medium or fast response which use a tight,

average or loose requirement to indicate a “stable”

( ) reading.

3.Select signal averaging: use to set the meter to

average readings that are very slow (10 readings),

slow (8),medium (6), fast (4) or very fast (2).

4.Set standardization delay: use to set a length of time

for the meter to wait before entering a relative mV

standardization.

5.Set mV offset: use to manually enter a mV offset

(same as in the mV Standardize menu).

6.Standardization menu: returns to the mV

Standardization Menu.

Clearing Relative mV Mode

Press Standardize,then press 3) Clear mV offset to clear

offset and return the meter to absolute mV mode.

mV Mode

Channel A: mV mode

Standardize Menu

1) Auto-enter mV offset

2) Set mV offset

3) Clear mV offset

4) Options menu

Channel A: mV mode

Options Menu

1) Select resolution

2) Select stability criteria

3) Select signal averaging

4) Set standardization delay

5) Set mV offset

6) Standardize

Quick Start Guide for Ion Measurements

1.Connect the Ion Selective Electrode (ISE) and

Reference Electrode,if required,to the

meter.”Combination”ISE’s have a reference

electrode built-in,and do not require a sepa-

rate reference electrode or connection.

2.Prepare two or more ion standards at concen-

trations bracketing typical sample solutions.

Add the appropriate Ionic Strength Adjuster

solution to each standard.

3.Set the meter to display the correct channel

(the channel with the ISE attached,either A or

B) using Channel.Set the meter to Ion mode:

press Mode,then 3) Ion.

Note: The meter will display “— — —” (no valid

data) until an ion standard has been entered.

4.Place the electrode(s) in the standard,provide

stirring (a magnetic stirrer is recommended),

and allow sufficient time (1 to 5 minutes

depending on the ISE) for the electrode to

reach a stable signal.

5.Press Standardize,1) Enter a standard and fol-

low the prompts.Repeat these steps to enter

up to seven ion standards.See the

Standardizing and Measuring Ion section for

more information.

6.Check the ISE response by pressing Cal Data to

see the standards and the ISE slope between

calibration points (standards).The meter will

allow an ion electrode slope between 5.92

mV/decade (10% slope) and 70.99

mV/decade (120% slope).

. A

f. A

. A

. B

Mode

Channel

ISA

Example: 1 mg/L 10 mg/L Ionic Strength

Adjuster

BNC

ref

measuring or

Combination ISE

reference

Cal Data

Channel A:

Select Mode

1) pH

2) mV

3) Ion

Channel A: ion mode

Ion Cal Data

1.00 mg/l 08/01/1998 08:32 AM

57.15 mV/decade

10.0 mg/l 08/01/1998 08:35 AM

Channel A: ion mode

Standardize Menu

1) Enter a standard

2) Set ion slope

3) Clear standards

4) Options menu

5) Cal reminder menu

Ion Mode Standardization Menu

Select channel A or B.Press Mode and then press 3) Ion for ion

mode.Press Standardize and the Ion Mode Standardize Menu

appears.

1.Enter a standard: use to add a new standard or update (re-

enter) an existing standard.Follow the prompts.With the first

standard you select the ion name and units.

2.Set ion slope: use to manually enter a slope for the selected

ion electrode.Used with a one-point ion calibration.Useful if

the ISE has a known,stable slope,so that measurements can

be made after entering a single ion standard.

Note: when two or more standards are entered,the meter

uses the actual determined slope(s).

3.Clear standards: use to clear standards for the electrode

standardization selected.

4.Cal reminder menu: use to set a timer reminding you to

recalibrate.A icon will appear on the main screen

and an exclamation mark will appear beside the standards

which need to be re-entered.

5.Options Menu: use to set various additional parameters to

the ion mode.See below.

Ion Mode Options Menu

1.Resolution: use to set the readings to 1,2,or 3 significant dig-

its.

2. Select stability criteria: use to set the stability criteria to slow,

medium or fast to match the electrode's speed and stability

of response,providing tight,medium and loose requirements

for a stable ( ) indication.

3.Select signal averaging: use to set filtering of the electrode

signal to very slow (10 readings),slow (8),medium (6),fast (4)

or very fast (2). Slower settings give more stable readings,

although may require longer times to reach stability.

4.Set standardization delay: use to set a reading delay time for

the meter to wait before accepting an electrode signal dur-

ing standardization.Programming a standardization reading

delay helps slow responding electrodes reach equilibrium

before the electrode signal is accepted.Delays of one

minute for fast ISE’s and five to ten minutes for slow ISE’s are

appropriate.

5.Set ion slope: use to enter a known ion electrode slope for a

one point standardization.

6.Standardize menu: returns to the ion standardize menu.

7.Manual temperature menu: use to set a manual temperature

for use in the absence of an ATC probe or when manually

overriding the ATC.

8.Data alarm menu: use to enter ion limits to be entered.If the

limits are exceeded an alarm indication (“

”) is displayed.

9.Set isopotential point: use to enter an isopotential point. See

page 20.

0.Enable incremental: use to turn on the known addition/sub-

traction type ion methods.See page 17.

Ion Mode

Channel A: ion mode

Standardize Menu

1) Enter a standard

2) Set ion slope

3) Clear standards

4) Options menu

5) Cal reminder menu

Channel A: ion mode

Options Menu

1) Select resolution

2) Select stability criteria

3) Select signal averaging

4) Set standardization delay

5) Set ion slope

6) Standardize menu

7) Manual temperature menu

8) Data alarm menu

9) Set isopotential point

0) Enable incremental modes

CAL!

Standardizing and Measuring Ion

1.Set the meter to ion mode (use Mode) and turn

ON the channel (use Channel) with the Ion

Selective Electrode (either Channel A or B).

The meter displays “— — —”, indicating no valid

measurement,until at least one ion standard has

been entered.

2.Prepare a standard,and add the appropriate

Ionic Strength Adjuster (ISA) solution to the stan-

dard.

3.Immerse the electrode(s) in the solution and stir

continuously.

4.Press Standardize,select the correct channel if

prompted to do so,and select 1) Enter a stan-

dard to add a standard.If this is the first standard

to be entered,select the ion name and units.

Follow the prompts.Be sure to allow enough time

for the electrode to reach a stable signal.

Note: The default standardization delay for ion

mode is 30 seconds.This can be set by the user.

See Ion Mode Options Menu,page 15.

5.The meter waits for a stable signal and enters the

standard.The entered standard appears in the

display (in single channel mode).Alternatively,if

the signal is not stable,you can press Enter when

the reading stabilizes according to your toler-

ance criteria.The meter then enters the stan-

dard.

6.Repeat steps 2 through 5 to enter additional

standards.Up to seven standards can be

entered.With more than one standard,the meter

performs a diagnostic check on the electrode.

Helpful Hints:

• Provide stirring.

• Allow the electrode time to reach a stable read-

ing before entering the standard into the meter.

• To achieve better accuracy, standardize using at

least two standards, bracketing the expected

range of your samples.

• Standardize from low to high concentrations.

• Always use fresh standards.

• Use standards and samples near the same tem-

perature.

• Remember to add Ionic Strength Adjuster to

each standard and sample.

ion standards

log [ion]

Channel B: ion mode

A 25.0°C

No standards.

08/01/1998 08:40 PM

Channel B: ion mode

Ion Checklist

Prepare standard or sample.

Add Ionic strength Adjuster.

Rinse electrode.

Place electrode in solution.

Stir moderately.

Press Enter to continue.

Standardize

Channel A: ion mode

Standardize Menu

1) Enter a standard

2) Set ion slope

3) Clear standards

4) Options menu

5) Cal reminder menu

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Omega PHB-215, PHB-220, PHB-225, PHB-250 Owner's manual

Brand: Omega

Model: PHB-215, PHB-220, PHB-225, PHB-250

Category: Measuring, testing & control

Type: Owner's manual

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Omega PHB-215, PHB-220, PHB-225, PHB-250 Owner's manual

Omega PHB-215, PHB-220, PHB-225, PHB-250 Owner's manual

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