Thermo Fisher Scientific UB NA 380A/B Subject: Unplugging Glass Restrictors Owner's manual

Brand: Thermo Fisher Scientific

Model: UB NA 380A/B Subject: Unplugging Glass Restrictors

Type: Owner's manual

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© 1999 PE Biosystems

User Bulletin: ABI 380A/B

Nucleic Acid Synthesizers

Subject: Unplugging Glass

Restrictors

User Bulletin - Number 24

Models: All 380B and 380A with restrictor update

Unplugging Glass Restrictors

Unplugging Glass Restrictors

(Part Number 000984)

Lack of flow of all reagents to any column may indicate that the glass capillary

restrictor has become clogged. Although this part is easily replaceable, it may

often be unclogged. Two methods have been successful:

1. Sonicate in a warm water bath.

2. Boil in water.

Should these methods fail, a cleaning wire (Hamilton Company, Part Number

18300, Diameter 0.00350 inches) may be used to physically remove the clog. This

method is least desirable because it may alter the bore of the glass.

If these procedures are unsuccessful, the glass restrictors should be replaced. On

three-column instruments all three restrictors should be replaced at the same

time. Restrictors are supplied in sets of three which have been tested together

and determined to give the same flow rate. This ensures equal flow to all three

columns. Always verify the flow rate of each column.

This is done by choosing the " Control and Test Solenoid Valves" softkey on the

Model 380A or the "Manual Control" key on the Model 380B and entering Function

9 to deliver acetonitrile to a previously specified column. Remove the bottom Luer

fitting from the column and after 20 seconds of flow, collect the acetonitrile in a

graduated tube for exactly 2 minutes. The flow should be to 1.70 mL/min. ± 0.15

mL. On three column instruments measure the flow rates of all three columns

separately. If the three columns give similar flow rates but are not within the

correct range, the regulators need to be adjusted. If the three columns give

different flow rates, the restrictors need to be replaced. Call your local Applied

Biosystems representative if you need assistance.

Information subject to change without notice.

Last updated May 1996

About Our Products Applying Our Products Technical Resources Services Corporate Information Community

1 of 2 11/23/99 8:29 AM

User Bulletin: ABI 380A/B Nucleic Acid Synthesizers http://www.pebio.com/ab/techsupp/doclib/nasynth/38x/ub/html/UB24.html

2 of 2 11/23/99 8:29 AM

User Bulletin: ABI 380A/B Nucleic Acid Synthesizers http://www.pebio.com/ab/techsupp/doclib/nasynth/38x/ub/html/UB24.html

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Thermo Fisher Scientific UB NA 380A/B Subject: Unplugging Glass Restrictors Owner's manual

Brand: Thermo Fisher Scientific

Model: UB NA 380A/B Subject: Unplugging Glass Restrictors

Type: Owner's manual

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