You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care, maintenance

(certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate

contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize yourself with proper installation,

maintenance, and operation of the cabinet. Other reference and guideline materials are available through the following web sites.

The information contained in this manual is intended to reflect our current production standard configuration model along with

the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back flap of this

report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy on file. A

replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief

description of the information desired.

loading capacity. The Energy Saver ECM motor is controlled to airflow setpoints via a solid-state DC motor controller with

digital dual thermistor airflow sensors that provide an automatic compensation (constant volume control) for both filter

loading and line voltage variances.

The Laminar Flow Biosafety Cabinet, (BSC) is a product resulting from the development of the "laminar flow" principle and

the application of environmental controls as required in the field of biological research or chemical containment. The BSC,

when used with proper technique, is an effective laboratory aid in obtaining the optimum control over product quality

while reducing the potential for exposure of both product and personnel to airborne biological or particulate chemical

agents in low to moderate risk-hazard research and use of the BSC to work with chemicals and trace amounts of

radionuclides required as an adjunct to microbiological studies, as prescribed by the Center for Disease Control (CDC)

The model NU-427, Class II, Type B1 Laminar Flow Biosafety Cabinet is manufactured in two sizes:

4 ft. (1.2m) and 6 ft. (1.8m).

The exhaust volume and corresponding inflow velocity are displayed on the front panel. Preset lower and upper

alarm limits are factory set but can be field verified at any time. To ensure that adequate exhaust is available for a

dirty exhaust HEPA filter condition, the nominal exhaust readings should be attainable with the butterfly valve or

damper set at 60% open, with all other dampers in the system (duct) open.

Verify airflow sensor shroud is in place: Downflow

Verify configuration type selection for specific model * (see section 7.5.2)

Verify setpoints and alarm limits for specific model * (see section 7.5.2)

Perform BCS certification.

At a minimum, the following tests should be performed: HEPA filter leak test, Downflow velocity test, Inflow velocity

test, Airflow smoke patterns, Site installation assessment tests.

++ Based on cabinet with new filters running at 115VAC.

The Biosafety Cabinet Control (BSCC) system is designed to service the control requirements of the NU-427

following functions:

grid

voltage variances and other environmental effects. A digital differential velocity pressure glow grid in the exhaust

airstream monitors for exhaust volume and subsequent inflow velocity. Downflow velocity, exhaust volume and

inflow velocity are displayed on the TOUCHLINK LCD screen. The control system also monitors the sliding window

position with a micro switch for both window height and window closed positions.

The control system, through the use of the front panel, controls the on/off function of the LED and ultraviolet

lights (optional), outlets and DC ECM motor/blower. The control system also allows contact closure outputs for

interaction with HVAC systems to optimize environmental performance.