Thermo Fisher Scientific 1300 Series B2 BSC Operating instructions

Type
Operating instructions
1300 Series B2
Class II, Type B2 Biological Safety Cabinet
Operating Instructions 3848304 Rev. G
Copyright
These operating instructions are protected by copyright. Rights resulting thereof,
particularly reprint, photomechanical or digital postprocessing or reproduction,
even in part, are only allowed with the written consent of the Thermo Fisher
Scientific Inc.
This regulation does not apply to reproductions for in-plant use.
Trademarks
All trademarks mentioned in the operating instructions are the exclusive property
of the respective manufacturers.
Thermo Fisher Scientific Inc
Controlled Environment Equipment
401 Millcreek Road, P.O. Box 649,
Marietta, Ohio 45750-0649
United States
Part #3848304, Rev. G
ECO H795
Operating Instructions Safety Cabinet | 1300 Series B2
Contents
CHAPTER 1: INTRODUCTION ........................................................................... 1
Instruction of the Operating Personnel ................................................................. 2
Warranty ............................................................................................................... 2
CHAPTER 2: PREREQUISITES .......................................................................... 3
Space Requirements ............................................................................................ 3
Suggested Location .............................................................................................. 4
Exhaust Requirements ......................................................................................... 5
Electrical Requirements........................................................................................ 5
Service Line Requirements .................................................................................. 6
CHAPTER 3: GETTING STARTED ..................................................................... 7
Unpacking the Biosafety Cabinet ......................................................................... 8
Moving the Cabinet .............................................................................................. 8
Preparing the Biosafety Cabinet for Operation ..................................................... 8
Installing the Biosafety Cabinet on an Existing Work Surface .............................. 8
Installing the Biosafety Cabinet on a Base Stand ................................................. 9
Telescoping Base Stands ..................................................................................... 9
Connecting the Biosafety Cabinet to Utility Service Lines .................................. 10
Exhaust System Connections ............................................................................. 11
Exhaust System Requirements .......................................................................... 12
Drain Valve Installation ....................................................................................... 12
Initial Certification ............................................................................................... 13
CHAPTER 4: PERFORMANCE FEATURES AND SAFETY PRECAUTIONS .. 15
HEPA Filters ....................................................................................................... 15
ULPA Filters ....................................................................................................... 15
Laminar Airflow ................................................................................................... 17
Directional Airflow ............................................................................................... 18
Motor/Blower ...................................................................................................... 19
Airflow Sensor .................................................................................................... 20
Cabinet Air Intakes (Grilles), Ductwork & Air Balance Controls .......................... 20
Ultraviolet (UV) Lamp ......................................................................................... 20
Safety Precautions ............................................................................................. 21
CHAPTER 5: USING THE CABINET ................................................................ 23
Correct Use ........................................................................................................ 23
Incorrect Use ...................................................................................................... 23
System Reset Switch ......................................................................................... 24
Information Center .............................................................................................. 24
Status Area ......................................................................................................... 25
Performance Indicator ........................................................................................ 25
Icon Area ............................................................................................................ 25
Operating the Sliding Sash ................................................................................. 26
Starting the Biosafety Cabinet ............................................................................ 26
The Control Keypad ........................................................................................... 27
User Selectable Features ................................................................................... 32
Timer Operation ............................................................................................... 32
Interval Timer Operation ..................................................................................... 33
Stopwatch Timer Operation ................................................................................ 33
Menu Options ..... ............................................................................................... 33
UV Lamp Operation ..................................................................................... 33
UV Lamp Timer ........................................................................................... 34
UV Lamp Maintenance ................................................................................ 34
Light Operation ............................................................................................ 34
Light Maintenance ....................................................................................... 35
Light Hourmeter .......................................................................................... 35
Change Light Life ........................................................................................ 35
Reset Light Clock ........................................................................................ 35
Blower Operation ........................................................................................ 35
User Preferences ........................................................................................ 35
If An Exhaust Error Activates .............................................................................. 37
If An Airflow Alert Activates ................................................................................ 37
Resetting the Airflow Alert System ..................................................................... 37
Working in the Biosafety Cabinet ....................................................................... 38
Operating Instructions Safety Cabinet | 1300 Series B2
Contents
CHAPTER 6: MAINTAINING THE CABINET ..................................................... 40
Routine Maintenance Schedule .......................................................................... 40
Service Operations ............................................................................................. 40
Work Surface Removal ................................................................................ 40
Front Grille Removal .................................................................................... 40
Towel Catch Removal .................................................................................. 41
Front Panel Removal ................................................................................... 42
Changing the B2 Prefilter ............................................................................. 43
Changing the Fluorescent Lamps ................................................................ 43
Changing the Optional UV Lamp ................................................................. 43
Resetting a Circuit Breaker .......................................................................... 44
Storage ............................................................................................................... 44
CHAPTER 7: TROUBLESHOOTING ................................................................. 45
APPENDIX A: COMPONENTS .......................................................................... 48
APPENDIX B: DIMENSIONS ............................................................................. 50
APPENDIX C: SPECIFICATIONS ...................................................................... 51
Motor Specifications ............................................................................................ 51
Environmental Conditions ................................................................................... 51
APPENDIX D: ACCESSORIES .......................................................................... 52
Telescoping Base Stands ................................................................................... 52
Seismic Bracket Kit ............................................................................................. 52
Service Valve Kit ................................................................................................. 52
Ultraviolet Lamp Kits ........................................................................................... 52
IV Bar Kits ........................................................................................................... 52
Backdraft Damper ............................................................................................... 52
ULPA Supply Filters ............................................................................................ 53
APPENDIX E: QUICK CHART ........................................................................... 54
DECLARATION OF CONFORMITY ................................................................... 55
Figures
Figure 2-1 Preferred location for the Biosafety Cabinet ....................................... 4
Figure 3-1 Adjusting height of the base stand ...................................................... 9
Figure 3-2 Leveling the base stand .................................................................... 10
Figure 3-3 Connecting Biosafety Cabinet to service lines .................................. 11
Figure 3-4 Attaching drain assembly .................................................................. 13
Figure 4-1 HEPA filters ...................................................................................... 16
Figure 4-2 Downflow Velocity Profile ................................................................. 17
Figure 4-3 Inflow Volume or Average Inflow Velocity ......................................... 18
Figure 4-4 Motor/Blower recirculation ................................................................ 19
Figure 5-1 System reset switch .......................................................................... 24
Figure 5-2 Information Center LCD .................................................................... 24
Figure 5-3 Control Keypad ................................................................................. 27
Figure 5-4 Programming Menu Instructions ....................................................... 28
Figure 6-1 Front grille removal ........................................................................... 41
Figure 6-2 Towel catch removal ......................................................................... 42
Figure 6-3 Front panel removal ......................................................................... 42
Figure 6-4 Resetting a circuit breaker ................................................................ 44
Figure A-1 Service parts/replacement accessory parts ...................................... 49
Figure B-1 Dimensions ...................................................................................... 50
Operating Instructions Safety Cabinet | 1300 Series B2
1
1. Introduction
Dear User,
Congratulations on your purchase of a Thermo Scientific 1300 Series B2 Class
II, Type B2 biosafety cabinet. Your 1300 Series B2 biosafety cabinet has been
tested and certified in accordance to NSF/ANSI 49, and is designed to protect
the user, the environment, and your research from harmful substances and
cross-contamination. This user’s manual provides instructions on how to use
the 1300 Series B2 most effectively and safely.
The 1300 Series B2 offers a unique range of product features that will enhance
your safety and improve overall operating efficiency. Should you have any
questions on using this product or need further explanation of any of its features,
please contact the Thermo Fisher Scientific technical support group at the
phone numbers listed on the following pages.
Postal address USA
Thermo Fisher Scientific Inc.
401 Millcreek Road
Marietta, Ohio 45750
Enquiries from North America, Latin America and Asia Pacific
Phone +1 888-213 1790
Fax +1 740-373 4189
Operating Instructions Safety Cabinet | 1300 Series B2
2
1. Introduction
Instruction of the Operating Personnel
The operating instructions describe the safety cabinet 1300 Series B2, and
applies to the models:
4-foot models 1310 / 1311 / 1315 / 1316 / 1317 / 1318
6-foot models 1360 / 1361 / 1362 / 1363 / 1364 / 1365
This safety cabinet has been manufactured in keeping with the latest
technological developments and has been tested before delivery for its correct
function. It may, however, present potential hazards if it is not used according to
the intended purpose or outside of operating parameters. Therefore, the
following procedures must always be observed to prevent accidents:
The safety cabinet should be operated by authorized personnel who have
been trained on the use and maintenance of this product.
For any operation of this device, it is recommended that the operator
prepares clear and concise written instructions in the language of the
operating and cleaning personnel based on these operating instructions,
applicable safety data sheets, plant hygiene guidelines, and technical
regulations. Most importantly:
which decontamination measures are to be applied for the cabinet and
accessories,
which protective measures apply while specific agents are used,
which measures are to be taken in the case of an accident.
To ensure safety during the repair of the cabinet, servicing of the equipment
must be carried out by trained and authorized personnel.
Warranty
Thermo Fisher Scientific warrants the operational safety and functions of the
safety cabinet only under the condition that:
the device is operated and serviced exclusively in accordance with its
intended purpose and as described in these operating instructions,
the device is not modified,
only original spare parts and accessories that have been approved by
Thermo Fisher Scientific are used,
inspections and preventative maintenance are performed at the specified
intervals,
an installation certification is performed prior to the initial operation of the
device and a repeat certification is performed on the occasion of all
inspections and repairs.
The warranty is valid from the date of delivery of the device to the operator.
Operating Instructions Safety Cabinet | 1300 Series B2
3
2. Prerequisites
Before you install the Biosafety Cabinet, you need to prepare the site for
installation. Carefully examine the location where you intend to install the
cabinet. You must be certain that the area is level and of solid construction. In
addition, a dedicated source of electrical power must be located near the
installation site.
Carefully read this chapter to learn:
Location requirements.
Electrical power requirements.
Exhaust requirements.
Service utility requirements.
Space requirements.
Refer to Appendix C: Specifications, for complete biosafety cabinet electrical
and environmental conditions, specifications and requirements.
Space Requirements
The dimensions for the different models are shown in Appendix B: Dimensions.
Operating Instructions Safety Cabinet | 1300 Series B2
4
2. Prerequisites
Suggested Location
Note: The Biosafety Cabinet should be located away from traffic patterns,
doors, fans, ventilation registers, fume hoods and any other air-handling devices
that could disrupt its airflow patterns. All windows in the room should remain
closed. Figure 2-1 shows the preferred location for the Biosafety Cabinet.
Preferred location
Air register blocked or redirected
to prevent cabinet disruption
Alternate location
Figure 2-1
Operating Instructions Safety Cabinet | 1300 Series B2
5
2. Prerequisites
Exhaust Requirements
Before deciding on a location, examine it carefully to ensure that it is compatible
with the cabinet’s exhaust duct. The area directly above the cabinet’s exhaust
port should be clear of structural elements, water and utility lines, or other fixed
obstructions. There should be enough clearance to allow for the passage of a
10" PVC or stainless steel duct. Avoid cabinet locations that require either an
elbow directly on top of the cabinet’s exhaust connection or an excessive
number of elbows to clear other items. For a further discussion of the cabinet’s
exhaust system requirements, please go to Chapter 3: Getting Started.
Electrical Requirements
The different Biosafety Cabinet models have the following electrical
requirements:
Model # Requirements
1310, 1311 115 VAC, 60 Hz, 12 Amps
1315, 1316, 1317, 1318 230 VAC, 50/60 Hz, 6 Amps
1360, 1361 115 VAC, 60 Hz, 12 Amps
1362, 1363, 1364, 1365 230 VAC, 50/60 Hz, 6 Amps
Note: A dedicated outlet with a circuit breaker should be located as close
as possible to the right rear side of the cabinet, at a height even with, or
higher than, the top of the bench or stand.
Table 2-1
Operating Instructions Safety Cabinet | 1300 Series B2
6
2. Prerequisites
Service Line Requirements
All utility service lines should be ¼ inch O.D., brass, copper, or stainless steel,
and equipped with an easily accessible shut-off valve. If the service line
pressure exceeds 100 PSI, it must be equipped with a pressure regulator to
reduce the line pressure. Consult local regulations for materials, pressures and
techniques regarding gas services.
Note: The use of flammable gases or solvents should be avoided in the
Biosafety Cabinet. Open flame in the cabinet will disrupt the laminar airflow in
the cabinet and may damage the HEPA filters. Flammable gases or solvents
may reach explosive concentrations in the cabinet or ductwork. If you feel that
the procedure requires the use of an open flame or flammable materials, contact
the institution’s safety office.
The use of air or gases under high pressure should be avoided as they may
seriously disrupt the airflow patterns in the cabinet.
Operating Instructions Safety Cabinet | 1300 Series B2
7
3. Getting Started
Now that the installation is properly prepared, you are ready to inspect, install,
and certify the Biosafety Cabinet. This chapter covers how to:
Unpack and move the Biosafety Cabinet.
Install the cabinet.
Connect the electrical supply source.
Connect the service lines.
Connect to an exhaust system.
Arrange certification of the Biosafety Cabinet.
Tools required for installation the Biosafety Cabinet include two 1/2" wrenches, a
flat-blade screwdriver, a #2 Phillips screwdriver, and a carpenter’s level.
Note: The Biosafety Cabinet models weigh between 400–700 lbs. (182-318 kg).
The shipping pallet allows for lifting with a mechanical lift truck or floor jack. If
you must lift the product manually, use at least six (6) persons and follow safe-
lifting guidelines.
Note: The side panels must be removed to access the fasteners that secure the
Biosafety Cabinet to the pallet. DO NOT ATTEMPT TO LIFT THE BIOSAFETY
CABINET BY THE SIDE PANELS; DAMAGE WILL OCCUR.
Operating Instructions Safety Cabinet | 1300 Series B2
8
3. Getting Started
Unpacking the Biosafety Cabinet
Carefully remove the outer carton and inspect the cabinet for damage that may
have occurred in transit. If the Biosafety Cabinet is damaged, notify the delivery
carrier immediately and retain the entire shipment intact for inspection by the
carrier.
Note: United States Interstate Commerce Commission rules require that claims
be filed with the delivery carrier within fifteen (15) days of delivery.
Do not return goods without the prior authorization of Thermo Fisher Scientific.
Unauthorized returns will not be accepted.
If the cabinet was damaged in transit, you must file a claim directly with the
freight carrier. Thermo Fisher Scientific is not responsible for shipping
damages.
Do not discard the carton or packing material for the Biosafety Cabinet until all
of the components have been checked, installed and tested.
The cabinet is secured to the pallet in two places on each side. To access the
nuts and bolts, remove the side panels by removing and keeping the two Phillips
screws on both panels. Swing the front of each panel away from the Cabinet,
and lift it straight up to remove the panel from the cabinet.
Moving the Cabinet
Move the cabinet, attached to its pallet, by using a floor jack, or a furniture dolly
underneath the unit. DO NOT move the cabinet by tilting it onto a hand truck.
Preparing the Biosafety Cabinet for Operation
Installation instructions for the cabinet are attached to the sash of the Biosafety
Cabinet. If these instructions are missing or unclear, contact Product Service at
1-888-213-1790.
Installing the Biosafety Cabinet on an Existing Work
Surface
Note: The Biosafety Cabinet is very top heavy. Use caution when lifting or
moving it.
When installing the Biosafety Cabinet onto an existing work surface or
benchtop, ensure that the structure can safely support the combined weight of
the cabinet and any related equipment. The work surface should be at least as
wide as the Cabinet and 31 inches (787 mm) deep to properly support the unit.
A hole or notch may be cut in the supporting surface in the right front corner to
accommodate the drain valve.
Operating Instructions Safety Cabinet | 1300 Series B2
9
3. Getting Started
Installing the Biosafety Cabinet on a Base Stand
Thermo Scientific offers accessory Base Stands that effectively support the
weight of the cabinet. Stands for a B2 cabinet generally are not mobile, since it
is connected to an exhaust duct.
Telescoping Base Stands
Adjust the leg height before installing the cabinet on top of the stand. The height
can be set in 1-inch intervals between 27.5 to 34.5 inches (699 to 876 mm),
providing a Cabinet work surface height from 30.0 to 37.0 inches (762 to
940 mm). The Base Stands for each width Cabinet are listed in Table 3-1
below.
Width Base Stand w/Feet Model #
4' 3730402
6' 3730602
1. Before positioning the Telescoping Base Stand in its final location, adjust
the height. A plastic bag containing the mounting bolts for the Biosafety
Cabinet is shipped with the base stand. Remove and save it.
2. Select the height of the stand and slide four (4) leg extensions into base
stand corner posts and attach with 2 ¼ inch long bolt, flatwasher,
lockwasher and nut. Ensure that the same height hole is selected for each
leg. Tighten the leg bolts securely. See Figure 3-1.
Table 3-1
Figure 3-1
Operating Instructions Safety Cabinet | 1300 Series B2
10
3. Getting Started
3. CAUTION: Only lift and support the cabinet at the stainless steel sides and
back. Damage will occur if lifted or supported on the front edge. Move the
base stand into its final location. Using a carpenter’s level, adjust each
leveling foot until the stand is level in both planes as shown in Figure 3-2.
You are now ready to lift the Biosafety Cabinet onto its stand.
Connecting the Biosafety Cabinet to Utility Service
Lines
The service lines (if any) should be connected to the tube fitting(s) on the
outside of the liner wall as shown in Figure 3-3. To install the tubing, follow
these steps:
1. Ensure that the tubing is ¼ inch O.D., soft metal, and that the end has been
completely deburred.
2. Route the tubing from the rear of the cabinet, ensuring that it will line up
with the slot in the back of the side panel. The slot is located from 8.75 to
11.25 inches (222 to 288 mm) from the bottom of the cabinet.
Note: Make sure that the tube routing will not contact any electrical wires. DO
NOT loop service line tubing within the side panels of the Cabinet.
3. Make sure that the nut on the tube fitting is loose, but do not remove it.
Look inside the fitting to make sure the tube ferrule is there.
4. Push the tube into the fitting until it is properly seated. The tube will go
approximately ¾ inch (19 mm) into the fitting.
Figure 3-2
Operating Instructions Safety Cabinet | 1300 Series B2
11
3. Getting Started
5. Tighten the tube fitting nut hand tight and then, using a 7/16-inch wrench,
tighten it at least ¾ turn more.
6. Close the service valve in the Biosafety Cabinet and then slowly open the
shutoff valve on the service valve. Test all fittings for leakage. Tighten the
tube nut slightly if needed.
Tube fitting nut
Tube fitting
Seal fittings to cabinet
with silicone sealant
Exhaust System Connections
The Total Exhaust Biosafety Cabinets are a “Type B2 cabinet,” meaning they
direct their HEPA filtered exhaust air out of the laboratory.
Note: The B2 Biosafety Cabinet is designed to be connected to an
appropriate exhaust system. Do not attempt to operate it when it is not
connected to an appropriate exhaust system.
THE EXHAUST CONNECTION IS A SEALED EXHAUST SYSTEM FROM THE
TOP OF THE CABINET TO THE REMOTE BLOWER. THE EXHAUST
SYSTEMS SHOULD BE DEDICATED TO A SINGLE CABINET. THE CABINET
IS EQUIPPED WITH AN EXHAUST FLOW ALARM, SUCH THAT AN ALARM
SOUNDS, AND THE CABINET BLOWER SHUTS OFF IN THE EVENT OF
INSUFFICIENT EXHAUST FLOW.
If your research involves the use of toxic compounds or volatile materials,
contact your facility’s safety officer or Thermo Fisher Scientific to ensure that
your cabinet and its exhaust system are compatible with the materials you will
be working with.
Figure 3-3
Operating Instructions Safety Cabinet | 1300 Series B2
12
3. Getting Started
Exhaust System Requirements
The cabinet exhaust stack is sized to accept 10-inch (254 mm) stainless steel
ductwork. The exhaust system’s blower should be sized to handle the sum of:
1) The pressure loss for the building’s exhaust system (duct work) when it
operates at the Cabinet’s flow rate.
2) PLUS The initial operating loss of the cabinet (1.0 inches (25 mm) of
water for the 1310 through 1318 Series B2 cabinets, OR 1.4 inches (36
mm) of water for the 1360 through 1365 Series B2 cabinets.
3) PLUS, In order to get the maximum possible life out of the cabinet’s
exhaust filter, the system should be able to draw an additional 2.0
inches (51 mm) of vacuum.
EXAMPLE
A 1310 Series Cabinet is being connected to an exhaust system that has a
calculated loss 0.5 inches of water when operating at the Cabinet’s required flow
rate of 810 CFM. What should the design capacity of the system be?
ANSWER 0.5” Exhaust system loss
+ 1.0” Initial Cabinet loss
+ 2.0” Reserve for maximum exhaust filter life
= 3.5” Vacuum total at maximum load
NOTE: The exhaust system should be fitted with a backdraft damper (part
#3858800) to prevent the reversing of airflow in the system.
Drain Valve Installation
In order to prevent damage during shipping, the drain valve assembly has not
been installed. It is not required, but if desired, the valve should be installed
after the Cabinet is in its final location.
To install the valve assembly, follow these steps:
Note: The work surface is heavy. Use caution when handling it.
1. Lift the work surface out of the Biosafety Cabinet by lifting on the knobs at
the front of the work surface. Steady the work surface while pulling it
straight out the front of the cabinet.
2. Using a putty knife, remove and discard the stainless steel cover that is
sealed over the drain mounting holes. Scrape out remaining sealant that is
around the holes.
3. Apply a light coating of silicone sealant (user supplied) to the mounting
surface of the drain assembly. Attach the drain assembly under the bottom
of the cabinet as shown in Figure 3-4. Wipe off any excess sealant from the
cabinet bottom. Ensure that the center drain hole is unobstructed.
4. Make sure the drain valve is in the closed position.
5. Reinstall the work surface.
6. Allow the silicone sealant to cure for at least eight hours before exposing it
to liquid.
Operating Instructions Safety Cabinet | 1300 Series B2
13
3. Getting Started
NOTE: If desired, the drain
valve assembly attaches to the
underside of the cabinet
bottom.
Apply a light coat of silicone
sealant to this surface of the
connector, aligning the three
holes in the connector with the
three holes in the Biosafety
Cabinet liner.
Initial Certification
Prior to use, a qualified certifier should certify all Biosafety Cabinets. Under
normal operating conditions, the Biosafety Cabinet should be recertified at least
annually and when relocated or serviced. The certifier should perform the
following tests, as recommended in ANSI/NSF International Standard Number
49 in effect when the cabinet was manufactured:
Downflow Velocity Profile Test
Inflow Velocity Test
Airflow Smoke Patterns
HEPA Filter Leak Test
Figure 3-4
Operating Instructions Safety Cabinet | 1300 Series B2
14
3. Getting Started
Site Installation Assessment Tests
Vibration Test *
Noise Level Test *
Lighting Intensity Test *
*These tests are user comfort related tests and may be omitted at the
user’s or certifier’s discretion.
If you have any questions regarding certification agencies or need assistance in
locating one, contact Thermo Scientific at 1-888-213-1790.
NOTE!
Service with costs:
Unless certification was expressly called for in the
specification, quotes and/or purchase order, the cost for
this on-site testing is to be paid for by the customer.
Operating Instructions Safety Cabinet | 1300 Series B2
15
4. Performance Features and Safety Precautions
All 1300 Series B2 Biosafety Cabinets operate using the following principles:
Filtration and retention of particulates by High Efficiency Particulate Air
(HEPA) filter(s)
Laminar airflow
Directional airflow
The major components in a Biosafety Cabinet are:
The HEPA or optional ULPA filter(s)
The motor/blower to force air through the Cabinet
Cabinet air intakes (grilles), ductwork and air balance controls
HEPA Filters
HEPA filters are disposable, dry-type particulate filters. The filter material or
media is typically made of borosilicate microfibers formed into a thin sheet, in a
process similar to the production of paper. This sheet is folded, or pleated to
increase its surface area. The pleats are held in place by aluminum diffusers or
by beads of glue that add rigidity to the media pack. The pack is then set into a
frame, and sealed as shown in Figure 4-1.
The HEPA filter manufacturer establishes the efficiency of the filter by
challenging it with an aerosol of known particle size. The number of particles
that penetrate the filter are quantified, and this establishes the efficiency of the
filter. Thus, the filters used in the Thermo Scientific 1300 Series B2 Cabinets are
at least 99.99% efficient in removing particles 0.3 micron.
Note: The HEPA filter media is very fragile. DO NOT touch the media. If you
think the media of a HEPA filter is damaged, DO NOT USE THE CABINET.
Have the HEPA filter integrity tested by a certifier before using the cabinet.
Note: HEPA Filters are only effective against particulate material. Gases will
pass through the filter.
ULPA Filters
ULPA filters have the same properties as the HEPA filters described above
except ULPA filters are rated at 99.999% efficient in removing particles 0.1-0.2
or 0.2-0.3 micron.
Operating Instructions Safety Cabinet | 1300 Series B2
16
4. Performance Features and Safety Precautions
Filter frame
Polyurethane seal
between media pack
and filter frame
Filter
media
pack –
A continuous sheet
of filter media
Figure 4-1
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Thermo Fisher Scientific 1300 Series B2 BSC Operating instructions

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