Microtek PlateLyzer User manual

Brand: Microtek

Model: PlateLyzer

Type: User manual

Microtek PlateLyzer is a professional scanner designed specifically for capturing images of biochip reactions. It utilizes advanced optical, signal, and image processing technologies to quickly and accurately acquire image information from the reactions of biochips with samples and reagents. It generates simple reports that can be exported for further analysis and retrieval, making it an essential device for sample assaying in biomedical laboratories and research institutions. PlateLyzer offers high optical resolution, a wide optical density range, and high stability, ensuring accurate and consistent scan results. Its unique dual scanning technology improves the accuracy and reliability of sample detection, while multiple scan modes allow for scanning of transparent or opaque microplate and glass slides. Additionally, the fast scanning speed and efficient warm-up time make it suitable for processing a large number of samples quickly.

www.microtek.com

Scanner

User's Manual

2 PlateLyzer Scanner User's Manual

Preface

be reproduced or copied in any way, stored electronically, or translated into any

language, without the permission of Microtek International, Inc.

Trademarks

Microtek, ScanMaker, ArtixScan, ScanWizard and ColoRescue are trademarks

or registered trademarks of Microtek International, Inc. All other trademarks or

registered trademarks are the property of their respective holders. Specications,

software and hardware bundles are subject to change without notice. Not

responsible for typographical errors.

Disclaimer

The contents of this manual have been checked carefully for accuracy, and every

effort has been made to ensure that there are no errors. However, Microtek

makes no guarantee as to the accuracy of the contents of this document and

reserves the right to make changes to the contents without prior warning.

I49-005500, A

May 2023

Microtek International, Inc.

No. 6, Industry East Road 3, Hsinchu Science Park, Hsinchu, 30075, Taiwan

Tel: 886-3-5772155, Fax: 886-3-5772598, https://www.microtek.com

PlateLyzer Scanner User's Manual 3

FCC Compliance Statement

FCC NOTICE (Class A)

This device complies with Part 15 of the FCC Rules. Operation is subject to the

following two conditions: (1) this device may not cause harmful interference, and

(2) this device must accept any interference received, including interference that

may cause undesired operation.

This equipment (Model: MRS-1632U2P) has been veried and found to comply

with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against harmful

interference. This equipment generates uses and can radiate radio frequency

energy and, if not installed and used in accordance with the instructions, may

cause harmful interference to radio communications.

However, there is no guarantee that interference will not occur in a particular

installation. If this equipment does cause harmful interference to radio or

television reception, which can be determined by tuning the equipment off and

on, the user is encouraged to try to correct the interference by one or more of

the following measures:

• Reorient or relocate the equipment.

• Increase the separation between the equipment and receiver.

• Connect the equipment into an outlet on a circuit different from that to

which the receiver is connected.

• Consult the dealer or an experienced technician for help.

Any changes or modications not expressly approved by the party responsible for

compliance could void the user’s authority to operate the equipment.

4 PlateLyzer Scanner User's Manual

Safety Precautions

Before using your scanner, read the following safety guides carefully, which

detail the proper operation of the scanner and its accessories to prevent injuries

or damage to users or equipment.

Power Source and AC Power Cord:

• Use a power source only with a power rating specied on the marking label.

• Insert the plug completely into the outlet, as a loose connection may cause

arcing and result in re.

• Place and route the power supply cord such that it is not likely to be walked

on or pinched by items placed upon or against them, paying particular

attention to the cord near the power plugs, convenience receptacles, and at

the point where it exits from the outlet.

• When the scanner is left unattended and unused for long periods of time,

unplug it from the wall outlet.

Moving and Storing the Scanner:

• Always lock the scanner when shipping or moving the scanner. Quick stops,

excessive force, and uneven surfaces may cause the product to overturn

when moving.

• When locking or unlocking the scanner, do not turn the scanner upside

down.

• Do not place the scanner on any slippery, slanted, or unstable surface. The

product may slide or fall, causing serious injury to people as well as serious

damage to the product.

• Do not use this scanner near water. Never spill liquid of any kinds on the

product, or it may result in electric shock or other hazards.

• This scanner should be situated away from heat sources such as radiators,

heat registers, stoves, or other products (including ampliers) that produce

heat.

Service:

• When you need a repair service, unplug the scanner from the power outlet

and consult qualied service personnel.

• When replacement parts are required, use replacement parts that are

specied by the manufacturer or have the same characteristics as the

original parts.

PlateLyzer Scanner User's Manual 5

Table of Contents

Preface ................................................................................................2

FCC Compliance Statement ................................................................3

Safety Precautions ..............................................................................4

Knowing about Your Scanner ...............................................................7

Features........................................................................................... 7

System Requirements ........................................................................ 8

Taking a Closer Look ......................................................................... 9

Front/Rear View .......................................................................... 9

Initial Setup .......................................................................................10

Step 1: Unpacking Package Contents ................................................ 10

Step 2: Installing the Software.......................................................... 11

Step 3: Unlocking the Scanner .......................................................... 12

Step 4: Connecting the Scanner ........................................................ 13

To the Power Adapter ............................................................... 13

To the USB 2.0 Cable ................................................................ 14

Positioning Samples ..........................................................................15

Standard Holder .............................................................................. 16

Dual-Scan Holder ............................................................................ 17

Slide Tray ...................................................................................... 18

Scanning and Measuring Scenarios ...................................................19

Using MiBio-Probe Software ............................................................. 19

Scanning Samples ..................................................................... 20

Creating a Spot Frame Template ................................................. 23

Measuring and Exporting Data .................................................... 25

Periodic Scanning ...................................................................... 28

Appendix ...........................................................................................29

Troubleshooting .............................................................................. 29

Specications ................................................................................. 30

6 PlateLyzer Scanner User's Manual

PlateLyzer Scanner User's Manual 7

Knowing about Your Scanner

Microtek PlateLyzer is a professional scanner designed specically for capturing

images of biochip reactions. It utilizes advanced optical, signal, and image

processing technologies, along with the Microtek MiBio-Probe software, to

quickly and accurately acquire image information from the reactions of biochips

with samples and reagents. It generates simple reports that can be exported for

further analysis and retrieval, making it an essential device for sample assaying in

biomedical laboratories and research institutions.

Features

• High optical resolution: Equipped with a high-resolution of CCD that can reach up

to 1632 pixels per inch, the scanner is able to capture ne details in the imaging

of the biochips, helping you analyze and assay samples more accurately.

• Wide optical density range: With a wide optical density range of 0.0D to 3.4D,

the scanner is capable of capturing ne details in both highlight and shadow

areas, as well as accurately representing biochip imaging at different densities,

ensuring the quality of the scanned images.

• High stability: With a coefcient of variation (CV) value of 0.5% and a relative

range (R) value of 1%, this scanner offers excellent performance in terms of

accuracy and consistency of scan results. It is suitable for quantitative analysis.

• Dual scanning: The unique dual scanning technology improves the accuracy and

reliability of sample detection. By subtracting the edge distortion images from

two scans and combining the clear images from the middle region, it provides

more reliable and accurate sample image data.

• Multiple scan modes: Provides scanning of transparent or opaque microplate

and glass slides, and supports 8-bit or 16-bit grayscale and RGB color scanning.

This allows you to perform sample scanning and data analysis based on different

needs, thereby obtaining more accurate test results.

• Fast and efcient: It only takes 10 minutes to warm up before scanning. Each

microplate scanning time is less than 1 minute, allowing you to process a large

number of samples quickly and obtain data rapidly.

• Microtek-patented E.D.I.T.™ technology: This is a patented “glassless” scanning

system, built into the lower bay of Microtek’s dual media scanners, which can

avoid the adverse effects by glass, such as Newton rings and image distortion.

This technology ensures clear and distortion-free images during the scanning

process.

• Microtek MiBio-Probe™ software: It is a professional software that combines

image scanning and analysis capabilities, providing comprehensive control over

scanning and measurement of images. With it, users can scan and measure

multiple samples simultaneously, as well as perform independent multi-target

measurements on individual samples. Additionally, the software has a user-

friendly interface that makes it easy for anyone to use.

8 PlateLyzer Scanner User's Manual

System Requirements

In order to use your scanner, your computer must satisfy the following

requirements:

• DVD-ROM drive (for installing software)

• Color display with 24-bit color output capability

• 1GB RAM or more (2GB is recommended)

• Intel Core i3 processor PC or higher with a built-in USB port

• Microsoft Windows 10/11 (compatible with Windows 7/8)

PlateLyzer Scanner User's Manual 9

No. Names of Parts Functions of Parts

1 Scanner lid Used to cover the glass surface of the scanner.

2 Power button Used to turn the scanner on or off.

3 LED indicators POWER indicator (Green): Indicates the scanner is on or off.

Reective Ready indicator (amber):

Indicates if the scanner is ready to scan.

Transparency Ready indicator (amber):

Indicates if the scanner is ready to scan.

4 Scanning bed (Drawer) Used to place the holder with loaded microplate or glass slide.

5 USB 2.0 port Used to connect the scanner to a computer.

6 Power connector Used to connect the scanner to a power source.

Taking a Closer Look

Front View

Rear View

10 PlateLyzer Scanner User's Manual

Initial Setup

After unpacking your scanner package, follow the steps below for a quick and

easy installation process.

NOTE:

• Be sure to use the power adapter and USB cable came with the scanner

package.

• Do not place the scanner on any slippery, slanted, or unstable surface. The

scanner may slide or fall, causing serious injury to people as well as severe

damage to the scanner.

• Save all packing materials of the scanner, including the carton, if you have a

need to transport the scanner in the future.

Step 1: Unpacking Package Contents

After unpacking your scanner package, please check for the major components

listed below. Take note that the sample holder you received depends on the

conguration of the scanner you purchased.

Scanner

Power adapter

Slide Tray

Sample holder

Standard Holder

Dual-Scan Holder

Hi-Speed USB cable

Power cord

PlateLyzer Scanner User's Manual 11

Step 2: Installing the Software

Important: Do not remove the yellow sticker from your scanner until you are told

to do so. You must install all software before connecting your scanner.

Always close any open programs and turn off Anti-virus utilities before installing

software.

Please follow the steps below to load and install the required software for the

scanner:

1. Please visit the Microtek website at https://www.microtek.com.

2. Search for the keyword “PlateLyzer“ and locate the ”Download” page for the

product.

3. Download the necessary drivers and software marked as “Yes” in the

download list based on your operating system.

4. Once the download is complete, double-click on the downloaded le (*.exe)

to start the installation process.

5. After all the software and programs have been installed, restart your

computer.

NOTE: Please note that the le download process may require some time. The

duration may vary based on your internet speed, bandwidth, le size, and other

variables. We kindly ask for your patience until the download is complete.

If you experience any problems during the download process or require further

assistance, please contact Microtek Customer Service Department.

12 PlateLyzer Scanner User's Manual

Step 3: Unlocking the Scanner

Before using the scanner, the scanner should be unlocked. To unlock the

scanner, follow the steps below:

1. Remove the yellow “Unlock”

sticker from your scanner.

2. With the scanner power off,

tilt the front right corner of the

scanner to see the locking screw

at the bottom.

3. Using a screwdriver, push

and turn the locking screw

counterclockwise to the unlock

position.

When successfully unlocked,

the screw will push out a little,

protruding slightly from the

bottom of

Locked

Unlocked

If you have a need to transport the scanner, the scanner must be locked in

advance. To lock the scanner, follow the steps below:

1. Turn off the scanner if your

scanner is on.

2. Turn the scanner back on. The

scanner’s carriage will move

to the standby position in few

minutes.

3. When the indicators on the

front of your scanner stop

blinking, use a screwdriver, and

then push and turn the locking

screw clockwise to the locked

position.

When the screw has been

tightened, this indicates that

your scanner is locked.

4. Turn off your scanner. The

scanner is now ready for

transport.

PlateLyzer Scanner User's Manual 13

Step 4: Connecting the Scanner

To the Power Adapter

1. Connect the power adapter to the back of the scanner.

2. Plug one end of the power cord into the power adapter, and plug the other

end of the power cord into a wall outlet or other power source..

14 PlateLyzer Scanner User's Manual

To the Hi-Speed USB Cable

3. Connect one end of the USB cable to your computer.

4. Connect the other end of the USB cable to the scanner’s USB port.

Power button

5. Press the Power button ( ) at the

front panel of the scanner, and wait

for the lights to stop blinking and

stay on steady.

The system will detect your

scanner automatically.

PlateLyzer Scanner User's Manual 15

Positioning Samples

To scan microplates or glass slides with samples, please use the sample holder

provided in the scanner package. Microtek offers two types of microplate holders

and one slide tray for you to choose from. You can select and purchase the

appropriate holder based on the type of vessel with loaded samples. Before use,

please refer to the following sections on how to put the holder with the loaded

microplate or glass slide into the scanning bed (drawer) of the scanner.

Slide Tray

Standard Holder

Dual-Scan Holder

NOTE:

• Due to the potential presence of biological hazards and infectious

substances, it is crucial to handle samples with care when placing them into

the sample holder and scanner. To prevent infection in case of spillage, it

is necessary to wear gloves and handle the microplate or glass slide with

loaded samples carefully.

• Make sure that the Calibration strip on the holder is kept clear and free of

obstruction at all times; no material should ever be placed on this area.

• Please take note that the high temperature may cause damage to your

samples. Therefore, turn off the scanner and remove the holder containing

the samples from the scanner once you nish the scanning job.

16 PlateLyzer Scanner User's Manual

Standard Holder

The Standard Holder is used to hold the transparent microplate with samples.

1. Place the microplate into the frame of the Standard Holder.

2. Insert the holder with loaded microplate all the way into the scanning bed

(drawer) of the scanner.

Keep Calibration strip clear

and free of any obstruction

at all times

PlateLyzer Scanner User's Manual 17

Keep Calibration strip clear

and free of any obstruction

at all times

Dual-Scan Holder

The Dual-Scan Holder is used to hold the transparent or opaque microplate with

samples.

1. Locate the spring latch on the corner of the holder, push it in the direction

as shown in the picture, and then place the microplate into the frame of the

holder.

Push and hold the spring latch

while placing the microplate.

NOTE: The Dual-Scan Holder uses the slide rail to move the microplate left

and right for scanning. You can place the microplate on either side of the

holder, and the scanner will automatically detect the microplate's position

and perform the left and right scans to complete the dual scanning action.

2. Insert the holder with loaded microplate all the way into the scanning bed

(drawer) of the scanner.

18 PlateLyzer Scanner User's Manual

Slide Tray

The Slide Tray is used to hold the glass slide with samples and can accommodate

up to four slides at a time. It must be used together with the Standard Holder for

scanning samples.

1. Put the glass slide to be scanned inside of the Slide Tray. Then, place the

Slide Tray into the frame of the Standard Holder.

Hold up to four glass

slides at a time

2. Insert the holder with loaded glass slides all the way into the scanning bed

(drawer) of the scanner.

Keep Calibration strip clear

and free of any obstruction

at all times

PlateLyzer Scanner User's Manual 19

Scanning and Measuring Scenarios

Using MiBio-Probe Software

Microtek MiBio-Probe is a program to acquire and measure images of biochips

with a variety of scanning, viewing and measurement functions. The software

allows users to quickly and accurately acquire image information from the

reaction of biochips with samples and reagents, and generate simple reports for

export to other software for analysis.

NOTE: Before using the Microtek MiBio-Probe for scanning, please ensure that

the Microtek ScanWizard Bio software has been installed on your computer.

The following sections provide usage scenarios for acquiring and measuring

images of biochips, including:

• Scanning Samples: Describes how to capture images of biochips and export

images.

• Creating a Spot Frame Template: Explains how to frame the position of the

analyte in the wells and create a spot frame template that can be shared

between different samples.

• Measuring and Exporting Data: Introduces how to measure the analytes in

the wells and export the measurement data for subsequent analysis.

• Periodic Scanning: Explains how to use the scanner to capture a series of

images from the same batch of samples at a set interval. This is useful for

tracking changes in the same sample over time.

Microtek

MiBio-Probe

20 PlateLyzer Scanner User's Manual

Scanning Samples

Before proceeding to templates and

measurement operations, follow these steps

to complete the sample scan:

1. Please refer to section the “Positioning

Samples” to place the sample holder

with the loaded microplate or glass slide

into the scanner's scanning bed (drawer).

NOTE: Make sure that nothing is on the

scanner's glass surface.

2. Double-click on the Microtek MiBio-Probe icon on the desktop to launch it.

3. Click on the Scan Settings button( ) to open the Settings window. Then

set the desired scanning parameters according to your needs.

a) In the Assay list, select a scan preset or a custom scan setting.

– None: The scan settings can be customized.

– Generic: The scan preset is the system preset.

If necessary, you can customize the scan presets via the Management

button next to the drop-down list and display them in the Assay option

for future use.

b) Custom Scan Settings:

– In the Tray Type list, select the type of sample tray to use.

– In the Scan Mode list, select Transparent or Reective according to

the type of microplate tray used.

– In the Resolution list, select the desired image output resolution.

– In the Color Type list, select the color mode for the image output.

– If necessary, select the direction and angle of image rotation in the

Rotate list.

– If necessary, adjust the Exposure Time slider.

c) Click on the OK button to return to the main interface.

4. Click on the Scan button to start the scanning process.

The system automatically saves the original scanned image (RAW le) in

the default folder and displays it as a thumbnail in the Thumbnail area. The

scanned image is also displayed in the Viewer panel.

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Microtek PlateLyzer User manual

Microtek PlateLyzer User manual

Microtek PlateLyzer User manual

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