Gilson FC 203B Quick Reference Manual

Brand: Gilson

Model: FC 203B

Type: Quick Reference Manual

Gilson,Inc.

3000 Parmenter Street • PO Box 620027 • Middleton, WI 53562 USA

Tel: 608-836-1551 or 800-445-7661 • F 608-831-4451 • www.gilson.com

FC 203B Fraction Collector

Quick Reference Guide

TABLE OF CONTENTS

FC 203B FraCtion ColleCtor | QUICK REFERENCE GUIDE

Table of Contents

Overview .................................................................................. 1

Customer Service ................................................................ 2

Notable Features ................................................................. 2

Collection Modes ......................................................... 2

Racks ................................................................................ 2

Operation ........................................................................ 3

Memory ............................................................................ 3

HELP ................................................................................. 3

Cycle Collection ............................................................3

Collection Windows .................................................... 4

Key Controls .......................................................................... 5

“Hard” Keys ....................................................................5

Cycle Collection ...................................................................11

Collection Modes ................................................................12

Time Mode .................................................................... 12

Drop Mode ..................................................................... 12

Manual Mode ................................................................13

Peak Modes ...................................................................13

Racks ...................................................................................... 16

Input/Output Control .......................................................18

Overview

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

Overview

This quick reference guide provides a concise overview

of the operating features of the fraction collector. In most

cases, this is the only documentation needed to operate

the fraction collector.

NOTE

This guide does not detail installation

procedures! Before operating the fraction

collector, refer to the user’s guide to make

all necessary plumbing and electrical

connections.

Use the quick reference guide for information about:

• control keys

• operating modes

• programming

• available racks

• input/output control

For more detailed information, refer to the user’s guide. It

contains instructions for:

• choosing run parameters

• making plumbing and electrical connections

• setting collection windows to selectively save or

discard effluent

• programming the fraction collector to control another

device

• using hidden function keys to set parameter values

and to monitor fraction collector performance

• maintaining and troubleshooting your unit

CUSTOMER SERVICE | FC 203B FRACTION COLLECTOR

CustOmer serviCe

Customer Service

Gilson, Inc. and its worldwide network of authorized

representatives provide customers with the following

types of assistance: sales, technical support, applications,

and instrument repair.

If you need assistance, please contact your local Gilson

representative. Specific contact information can be found

at www.gilson.com.

Notable Features

Collection Modes

Choose from several collection modes: time, drop,

peak plus time, peak plus drop, or manual. Refer to

Collection Modes on page 12 for more information.

Racks

Choose from a variety of racks. Each rack has a code

number. Tell the fraction collector which rack you

are using by entering the rack code number. When

changing racks, simply enter the new code. See Racks on

page 16 for more information.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

CustOmer serviCe

Operation

The fraction collector has two kinds of controls:

• “Hard” keys, such as START, END, YES, NO. The

functions of these keys do not vary.

• “Soft” keys, the four white keys below the display.

The functions of the soft keys vary depending on the

current display. The current function of each soft key

appears on the display directly above the key.

See Key Controls on page 5 for more information.

Memory

The fraction collector remembers its last set of

instructions (e.g., the operating mode and the numerical

values assigned to the various options) even if the power

has been turned off. It always “wakes up” ready to do

exactly what it did the last time.

At each startup, check the list of current values. To check

the values in the current mode, press List.

HELP

Pressing this hard key provides on-line instruction during

setup or during a run.

Cycle Collection

The fraction collector allows for collecting fractions from

each sample into the same set of tubes or into a new set

of tubes.

CUSTOMER SERVICE | FC 203B FRACTION COLLECTOR

CustOmer serviCe

Collection Windows

Collection windows allow for collecting only the effluent

desired and discarding the rest. Optionally, build up to ten

collection windows.

Collection windows are useful for:

• excluding void volumes

• excluding equilibration or washing volumes

• aiding in selective peak collection

Remember four key points about collection windows:

• It is always possible to collect from start to end,

designating the entire collection period as one

collection window.

• Whenever the fraction collector is outside a collection

window, it automatically diverts the effluent to drain.

• At the start of each collection window, the dispense

head automatically advances to the next tube.

• The collection windows are not mode specific.

Windows created in one mode are active in all other

modes.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

Key COntrOls

Key Controls

“Hard” Keys

START

Key used to start collection. This key has the same

function as the Start Soft Key. It is also possible to start

the fraction collector remotely; refer to the user’s guide.

END

A manual override key that ends the collection and

returns the dispense head to the “home” position at the

left and rear of the unit. Typically, the dispense head will

return to “home” after collecting into the last tube in the

rack.

When using the multiple column option, press END after

the last fraction has been collected.

If END is pressed while viewing any display, the ready-

to-run display of the current mode will appear. It is then

possible to begin collecting fractions.

EDIT

Key that calls up the display you use to select or change

collection modes. To access advanced technical and

service options, press EDIT 1, 2, or 3. Refer to the

appendices in the user’s guide for more information

about those options.

KEY CONTROLS | FC 203B FRACTION COLLECTOR

Key COntrOls

HELP

Displays information and instructions to simplify

operation during setup or during a run. There are many

help messages. Each gives specific information about the

current options.

ADV

Manually advances the number of the current collection

vessel. The current tube number is always shown on the

display. For instance, if tube 1 is the current collection

vessel, pressing ADV makes tube 2 the current collection

vessel. When using the multiple column option, the

current fraction number, rather than tube number, is

displayed.

Typically, tube advance is automatic, but it is possible to

manually initiate an advance by pressing ADV or advance

remotely, as described in the user’s guide.

To understand the interaction between the

ADV and DRAIN Keys, refer to DRAIN below.

DRAIN

Diverts effluent to drain. DRAIN is a two-state key. Press

it once, its light goes on and effluent is diverted to drain.

Press it a second time, the light goes off and the fraction

collector collects into the current vessel.

Whenever the dispense head of the fraction collector

travels from a vessel to the drain trough or back again, it

avoids passing over any vessel into which fractions have

been collected, to prevent contamination of collected

fractions.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

Key COntrOls

While the fraction collector is draining, pressing ADV

does not resume collection, but rather it advances the

number of the current tube, which is always displayed

during a run. When DRAIN is pressed a second time, the

fraction collector collects into the vessel shown on the

display. This allows for maximum flexibility: after draining,

optionally resume collection in the last vessel, the next

one, or the first vessel in the next row.

0–9

Press number keys to enter numerical values. The decimal

point, where applicable, does not have to be entered.

One easy way to enter a new number is to press the NO

key to clear the current number and then enter the new

number. Numbers appear on the display at the far right,

and move to the left with each successive entry.

YES

Press YES when you want the instructions shown on the

top line of the display to apply. The YES Key has the same

function as the Ok Soft Key.

Press NO to clear the numerical value from the

display. Also used to choose alternate options (e.g.,

programmable output 2).

KEY CONTROLS | FC 203B FRACTION COLLECTOR

Key COntrOls

“Soft” Keys

The following functions are controlled by the four white

keys on the fraction collector front panel. Directly above

a soft key its current function is displayed. If the display

above a key is blank, that key is inactive.

Add

Press to begin building a collection window. Press Add

and then specify the start and end times of the window.

When the fraction collector is outside a window, all

effluent will be discarded.

Collection windows are optional. Add up to ten windows.

Refer to the user’s guide for detailed instructions.

Collect

Press to collect the non-peak effluent in peak modes.

Collection of non-peak material is optional. It is possible

to set a different time/tube or drop/tube value for

collection of non-peak effluent than for collection of peak

effluent. Refer to Discard on page 9.

Cycle

Identifies the next set of tubes to be used for collection.

This soft key appears instead of the Start Soft Key if the

Tubes per cycle Parameter was set to a number greater

than 0 (zero). Refer to the user’s guide for detailed

instructions.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

Key COntrOls

Del

Press to delete the collection window currently shown on

the top line of the display. To collect from start to end,

delete all collection windows. Refer to the user’s guide for

detailed instructions.

Delayed Adv

In manual mode, this soft key functions like ADV, except

tube advance is delayed to account for the time it

takes effluent to pass from the detector to the fraction

collector.

Discard

In peak modes, press to divert the non-peak effluent to

the drain.

Drop

Specifies collection in drop mode. Refer to

Collection Modes on page 2 for more information.

Back

After viewing a HELP message, press this key to return to

the point at which HELP was requested.

List

Press to display a listing of each option and its current

value in the selected collection mode.

Manl

Specifies collection in manual mode. Refer to

Collection Modes on page 2 for more information.

KEY CONTROLS | FC 203B FRACTION COLLECTOR

Key COntrOls

Press this key to see the next display of a HELP message.

Refer to Back on page 9.

Press to apply the instructions shown on the top line of

the display. Works in the same way as the YES Hard Key.

Peak

Specifies collection in one of the two peak modes.

After making this selection, select to subfractionate

peaks by time or by drop. Refer to Collection Modes on

page 2 for more information.

Quit

Press to return to the previous display.

Rack

Press to tell the fraction collector which rack will be

used. Change the rack code by entering a new number.

Also calls up a description of the rack code that

currently appears on the display. Refer to Racks on

page 16 for more information.

Start

Press to start the collection. The START Hard Key has the

same function.

Time

Specifies collection in time mode. Refer to

Collection Modes on page 2 for more information.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

CyCle COlleCtiOn

Cycle Collection

The fraction collector enables collecting fractions two

different ways:

• Collect each sample, or full collection cycle, into the

same set of tubes. During the run, each cycle begins at

tube 1.

• Collect each sample, or full collection cycle, into a new

set of tubes. During the run, each cycle begins at the

tube following the last tube of the previous cycle.

The Tubes per cycle Parameter in the Technical Menu

(press EDIT 2) allows for indicating how the fractions will

be collected.

To collect each sample into the same set of tubes, set the

Tubes per cycle Parameter to 0 (zero).

To collect each sample into a new set of tubes, set the

Tubes per cycle Parameter to a value other than 0 (zero).

Using the tubes per cycle and the total tube capacity for

the rack, the number of cycles is calculated. If the number

of tubes per cycle cannot be divided evenly into the tube

capacity for the rack, the number of cycles is the whole-

number quotient; the remaining tubes are not used.

Refer to the user’s guide for more information.

COLLECTION MODES | FC 203B FRACTION COLLECTOR

COlleCtiOn mOdes

Collection Modes

Time Mode

For collection of timed fractions; from 0.01 to

99.99minutes per tube, in 0.01 minute increments.

Parameters:

_ _ . _ _ min/tube

Collect: _ _ _ . _ to _ _ _ . _ (collection windows)

Optionally, change min/tube values during the run. Enter

new numbers and then press YES.

Drop Mode

For collection of fractions by drop count; from 1 to

9999drops per tube.

Parameters:

_ _ _ _ drops/tube

Collect: _ _ _ . _ to _ _ _ . _ (collection windows)

Optionally, change drops/tube values during the run.

Enter new numbers and then press YES.

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

COlleCtiOn mOdes

Manual Mode

For manual collection, use the START, ADV, DRAIN and

END Keys. A delayed advance soft key advances the

dispense head only after waiting until the effluent passes

from the detector to the fraction collector. Refer to the

user’s guide for more information about when to activate

the delayed advance function.

Parameters:

Delay time: _ . _ _ min

Collect: _ _ _ . _ to _ _ _ . _ (collection windows)

Peak Modes

Choose to collect peak and/or non-peak fractions. To

detect peaks, the fraction collector must be connected

to an analog output of a detector. The fraction collector

can interpret the detector data to distinguish peaks from

non-peaks. Refer to the user’s guide for instructions

to connect the detector to the fraction collector

multipurpose I/O port.

There is no peak-only mode. Peak or non-peak effluent

must be subdivided either:

• by time (from 0.01 to 99.99 min/tube) or

• by drops (from 1 to 9999 drops/tube)

COLLECTION MODES | FC 203B FRACTION COLLECTOR

COlleCtiOn mOdes

In peak modes, the fraction collector needs the following

information:

• the width of a typical peak in the run at half of its

maximum height, measured in minutes

• the height of the shortest peak of interest, measured

in millivolts (this number is easily determined from

the full scale voltage deflection of the detector and

recorder) or the millivolt (mV) level above which peaks

will be collected

• the time it takes for the effluent to pass from the

detector flow cell to the dispense head of the fraction

collector. This aids in the collection of purer peaks. The

user’s guide explains how to calculate the delay time

based on the setup.

Optionally, collect the non-peak effluent at a different

time/tube or drop/tube than the peak effluent.

To discard non-peak effluent to waste, press the

Discard Soft Key. When you discard non-peak effluent, it

is highly recommended that you use a 3-way valve. Refer

to the user’s guide.

Parameters:

Peak: _ _ . _ _ min/tube

Peak: _ _ _ _ drops/tube

Non-peak: _ . _ _ min/tube

Non-peak: _ _ _ _ drops/tube

FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE

COlleCtiOn mOdes

NOTE

The “Non-peak” displays will not appear if

discard non-peak fractions was selected.

Peak width: _ . _ _ min

Peak height: _ _ . _ mV

Peak level: _ _ . _ mV

Delay time: _ . _ _ min

Collect: _ _ _ . _ to _ _ _ . _ (collection windows)

It is possible to change time/tube or drop/tube values

during a run. Enter numbers and then press YES.

RACKS | FC 203B FRACTION COLLECTOR

raCKs

Racks

The fraction collector “knows” the collection patterns

for all of the following racks, according to their code

numbers. When you enter the code number of the rack

you intend to use, the fraction collector describes the

rack to you. To change rack codes, simply enter the code

of the new rack.

RACK DESCRIPTION

Collection Plate 80-cavity plate, 2.5 mL/cavity; will also hold 11mm

Eppendorf vials. Set the rack code to “0”.

Code 0 rack For 80 12 mm diameter vials

Code 1 rack For 80 13 mm diameter tubes

Code 2 rack For 119 6 mm diameter tubes

Code 4 rack For 80 12 mm diameter tubes

Code 11 rack For 105 13 mm diameter tubes

Code 14 rack For 128 12 mm diameter tubes

Code 15 rack For one 96-well microplate. Set rack code to “15” to

collect across rows. Set rack code to “17” to collect in

a serpentine pattern.

Code 20 rack For 108 10 mm diameter tubes

Code 21 rack For 60 13 mm diameter tubes

Code 23 rack For forty-four 17 x 55 mm (6.8 mL) or 17x65mm

(8mL) mini-scintillation vials

Code 23W rack For 44 Waters WISP vials. Set rack code to “23”.

Page is loading ...

Gilson FC 203B Quick Reference Manual

Gilson FC 203B Quick Reference Manual

Gilson FC 203B Quick Reference Manual

Related papers

Other documents