Thermo Fisher Scientific HIGHPlate 2x5 Rotor User manual

Thermo Scientiﬁc

Highplate Rotor 2 x 5

Instruction Manual

50134653-b • 07 / 2020

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This product is subject to the regulations of the EU Waste Electrical & Electronic

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Thermo Scientific 75006444 Highplate Rotor 2 x 5 i

T

Preface ........................................................................................................................................ ii

Scope of Supply ........................................................................................................................ iii

Precautions ...............................................................................................................................iv

Chapter 1 Rotor Data ................................................................................................................................ 1-1

Chapter 2 Rotor Installation ................................................................................................................... 2-1

Rotor Installation ..................................................................................................... 2-2

Removing the Rotor ................................................................................................ 2-2

Chapter 3 Rotor Loading .......................................................................................................................... 3-1

Before a Run ............................................................................................................ 3-2

Proper Loading ........................................................................................................ 3-2

Improper Loading .................................................................................................... 3-2

Handling Micro Plates ............................................................................................. 3-3

Chapter 4 Aerosol-tight Applications ................................................................................................... 4-1

Basic Principles ........................................................................................................ 4-2

Fill Level .................................................................................................................. 4-2

Checking the Aerosol-Tightness .............................................................................. 4-2

Chapter 5 Maintenance and Care ......................................................................................................... 5-1

Cleaning Intervals .................................................................................................... 5-2

Cleaning .................................................................................................................. 5-2

Disinfection ............................................................................................................. 5-3

Decontamination ..................................................................................................... 5-4

Autoclaving .............................................................................................................. 5-5

Service of Thermo Fisher Scientific .......................................................................... 5-5

RCF-Values ...............................................................................................................A-1

Chemical Compatibility Chart ...............................................................................B-1

Table of Contents

Technical Data ........................................................................................................ 1-2

Thermo Scientific 75006444 Highplate Rotor 2 x 5 iii

P

Preface

Before starting to use the rotor, read through this instruction manual carefully and follow the

instructions.

The information contained in this instruction manual is the property of Thermo Fisher Scientific; it

is forbidden to copy or pass on this information without explicit approval.

Failure to follow the instructions and safety information in this instruction manual will result in the

expiration of the sellers warranty.

Scope of Supply

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.

Article Number Quantity Check

75006444 Highplate 2 x 5 1

76003500 Grease for rubber sealing 1

75003786 Bolt grease 1

50134653 Instruction manual 1

This symbol refers to general hazards.

WARNING means that injuries or material damage or contamination could occur.

CAUTION means that material damage could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your

environment safe.

Preface

iv75006444 Highplate Rotor 2 x 5 Thermo Scientific

Precautions

In order to ensure safe operation of the Highplate rotor 2 x 5, the following general safety

regulations must be followed:

•Do not remove the magnet at the rotor bottom.

•Do not use rotors which show any signs of corrosion and/or cracks.

•Use only with rotors which have been loaded properly.

•Never overload the rotor.

•Operate the rotor always with the lid closed.

•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this

rule are commercially available glass or plastic centrifuge tubes, provided they have been approved

for the speed or the RCF value of the rotor.

•Please observe the safety instructions.

Please pay particular attention to the following aspects:

•Rotor installation: Check that the rotor is locked properly into place before operating the

centrifuge.

•Always balance the samples.

Maximum sample density at maximum speed: 1.2

g

cm3

-----------

Thermo Scientific 75006444 Highplate Rotor 2 x 5 1-1

1

Rotor Data

Contents

•“Technical Data” on page 1-2

1 Rotor Data

1-2 75006444 Highplate Rotor 2 x 5 Thermo Scientific

Technical Data

Table 1-1. 230 V, 50 / 60 Hz

Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR

Catalog Number 75004471 75004479 75004461

Weight of empty Rotor [ kg ]14 14 14

Maximum permissible Load [ g ] 2 x 500 2 x 500 2 x 500

Maximum Speed nmax [ rpm ] 5650 5650 5650

Maximum RCF-Value at nmax 5889 5889 5889

Radius max. / min. [ cm ] 16.5 / 10.5 16.5 / 10.5 16.5 / 10.5

Acceleration / Braking Time [ s ] 95 / 100 95 / 100 80 / 100

Sample Heating at nmax [ °C ] referred to Ambient

Temperature of 23-25 °C, Run Time 60 Minutes

00

*0

Maximum Speed nmax [ rpm ] at 4 °C 5650 5650 5650

Aerosol-tight** Yes Yes Yes

Autoclavable No No No

*at 200 V, 50 Hz up to 3 °C

**tested by TÜV Product Service GmbH - Hamburg

Table 1-2. 120 V, 60 Hz

Centrifuge Cryofuge 5500i

Catalog Number 75004475

Weight of empty Rotor [ kg ]14

Maximum permissible Load [ g ] 2 x 500

Maximum Speed nmax [ rpm ] 5650

Maximum RCF-Value at nmax 5889

Radius max. / min. [ cm ] 16.5 / 10.5

Acceleration / Braking Time [ s ] 95 / 100

Sample Heating at nmax [ °C ] referred to Ambient

Temperature of 23-25 °C, Run Time 60 Minutes

0

Maximum Speed nmax [ rpm ] at 4 °C 5650

Aerosol-tight*

*tested by TÜV Product Service GmbH - Hamburg

Yes

Permissible Temperature Range autoclavable °C No

1 Rotor Data

Thermo Scientific 75006444 Highplate Rotor 2 x 5 1-3

Table 1-3. 230 V, 50 / 60 Hz

Centrifuge Sorvall RC-4

Catalog Number 75004473 75004481

Weight of empty Rotor [ kg ]14 14

Maximum permissible Load [ g ] 2 x 500 2 x 500

Maximum Speed nmax [ rpm ] 5650 5650

Maximum RCF-Value at nmax 5889 5889

Radius max. / min. [ cm ] 16.5 / 10.5 16.5 / 10.5

Acceleration / Braking Time [ s ] 95 / 100 95 / 100

Sample Heating at nmax [ °C ] referred to Ambient

Temperature of 23-25 °C, Run Time 60 Minutes 00

*

Maximum Speed nmax [ rpm ] at 4 °C 5650 5650

Aerosol-tight** Yes Yes

Permissible Temperature Range autoclavable °C No No

*at 200 V, 50 Hz up to 3 °C

**tested by TÜV Product Service GmbH - Hamburg

Table 1-4. 120 V, 60 Hz

Centrifuge

Catalog Number 75004477

Weight of empty Rotor [ kg ]14

Maximum permissible Load [ g ] 2 x 500

Maximum Speed nmax [ rpm ] 5650

Maximum RCF-Value at nmax 5889

Radius max. / min. [ cm ] 16.5 / 10.5

Acceleration / Braking Time [ s ] 95 / 100

Sample Heating at nmax [ °C ] referred to Ambient

Temperature of 23-25 °C, Run Time 60 Minutes

3

Maximum Speed nmax [ rpm ] at 4 °C 5650

Aerosol-tight*

*tested by TÜV Product Service GmbH - Hamburg

Yes

Permissible Temperature Range autoclavable °C No

Sorvall RC-4

Technical Data

Thermo Scientific 75006444 Highplate Rotor 2 x 5 2-1

2

Rotor Installation

Contents

•“Rotor Installation” on page 2-2

•“Removing the Rotor” on page 2-2

2 Rotor Installation

Rotor Installation

2-2 75006444 Highplate Rotor 2 x 5 Thermo Scientific

Rotor Installation

Use only the rotors with approved accessories.

Proceed as follows:

1. Open the lid of the centrifuge and if necessary, remove any dust, foreign objects or residue from

the chamber.

Thread and O-ring must be clean and undamaged.

2. Hold the rotor over the centrifuge spindle.

3. Let the rotor slide slowly down the centrifuge spindle.

4. Thread the fastening tool into the centrifuge spindle clockwise. Hold the rotor with the other hand

into position.

5. Check if the rotor is properly installed by lifting it slightly on the handle.

6. If available close the rotor with the rotor lid.

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Thread the fastening tool in the centrifuge spindle counterclockwise. Hold the rotor with the

other hand into position.

3. At the same time, pull the rotor directly upwards with both hands and remove it from the

centrifuge spindle. Make sure not to tilt the rotor while doing this.

CAUTION Unapproved or incorrectly combined accessories can cause serious damage to

the centrifuge.

CAUTION Do not force the rotor onto the centrifuge spindle.

WARNING Check for any damage to the rotor: Damaged rotors must not be used.

Keep the centrifuge spindle area of the rotor clear of objects.

CAUTION Check that the rotor is properly locked on the centrifuge spindle before each

use by pulling it at its handle.

Be sure to check all sealings before starting any aerosol-tight applications.

See the information in the rotor instruction manual.

2 Rotor Installation

Removing the Rotor

Thermo Scientific 75006444 Highplate Rotor 2 x 5 2-3

Aerosol-tight Rotors

When using an aerosol tight lid the rotor can only be removed with the lid closed. This is to protect

you and the samples.

Thermo Scientific 75006444 Highplate Rotor 2 x 5 3-1

3

Rotor Loading

Contents

•“Before a Run” on page 3-2

•“Proper Loading” on page 3-2

•“Improper Loading” on page 3-2

•“Handling Micro Plates” on page 3-3

3 Rotor Loading

Before a Run

3-2 75006444 Highplate Rotor 2 x 5 Thermo Scientific

Before a Run

1. Please read and observe the safety instructions contained in this operating instruction and in the

instructions for use.

2. Check the rotor and all accessory parts for damages such as cracks, scratches or traces of corrosion.

3. Check the rotor chamber, the centrifuge spindle and the fixation of the rotor for damages.

4. Check the rotor’s suitability using the chemical compatibility chart on page B-1.

5. Make sure the tubes or bottles do not touch the bucket lid.

6. Check the rotor bolts and apply grease 75003786 before using for the first time.

7. Check that each bucket or micro-plate carrier can swing freely by moving it carefully with your

hand. Weigh the bucket content (adapter and tube). Make sure you do not exceed the maximum

compartment load.

Proper Loading

To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times.

Improper Loading

CAUTION Always be sure to use buckets of the same weight class. Operate the rotor always

with the lid closed.

3 Rotor Loading

Handling Micro Plates

Thermo Scientific 75006444 Highplate Rotor 2 x 5 3-3

Handling Micro Plates

•Always remove the plate holder (70057477) before loading and unloading it.

•The rubber cushion (20056846) should be in the plate holder.

•Let the plate holder (70057477) slide vertically into the bucket.

•Deepwell plates can be inserted without plate holder (70057477).

•Make sure the used micro plates can withstand the centrifugal forces.

Thermo Scientific 75006444 Highplate Rotor 2 x 5 4-1

4

Aerosol-tight Applications

Contents

•“Basic Principles” on page 4-2

•“Fill Level” on page 4-2

•“Checking the Aerosol-Tightness” on page 4-2

4 Aerosol-tight Applications

Basic Principles

4-2 75006444 Highplate Rotor 2 x 5 Thermo Scientific

Basic Principles

•Check that the sample containers are well suited for the desired centrifugation process.

Fill Level

The tubes are only to be filled to a level which ensures that the sample is unable to reach the top of the

tube during centrifugation. Therefore, fill the tube only 2/3 of the rated level.

Checking the Aerosol-Tightness

The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in

accordance with the EN 61010-2-020 Appendix AA.

Whether or not a rotor is aerosol-tight depends primarily on proper handling.

Check as needed to make sure your rotor is aerosol tight.

The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks,

scratches and embrittlement are extremely important.

Aerosol-tight applications are not possible if the lids are open.

Aerosol-tightness requires the correct operation when filling the sample vessels and closing the rotor lid.

•Lubricate all seals lightly.

Always use the special grease 76003500 when lubricating the seals.

•When you load a rotor before installing it in the centrifuge, make sure you do not tilt it when

installing.

CAUTION Aerosol-tight rotors and tubes may only be opened in an approved safety

work-bench when centrifuging dangerous samples.

Mind the maximum permissible load.

CAUTION Be sure to check all sealing before starting any aerosol-tight applications.

CAUTION This quick test is suited for validating the aerosol tightness of your

Highplate 2 x 5 rotor. Check the seals and sealing surfaces of the lid thoroughly.

CAUTION Prior to each use, the seals in the bucket are to be inspected in order to assure

that they are correctly seated and are not worn or damaged. Lubricate all seals lightly.

Damaged parts must be replaced immediately.

When loading the rotor, ensure that the rotor lid closes securely.

Damaged or clouded rotor lids are to be replaced immediately.

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Thermo Fisher Scientific HIGHPlate 2x5 Rotor User manual

Thermo Fisher Scientific HIGHPlate 2x5 Rotor User manual

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