WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked with the following symbol:
Thermo Scientific Hematocrit Rotor | 3
WEEE Compliance ...................................................2
Table of Contents ...................................................3
Preface ...........................................................5
Items Supplied .......................................................... 5
Intended Use ............................................................ 5
Precautions ............................................................. 6
Symbols used in the manual ................................................ 6
Rotor Specifications ................................................. 7
Thermo Scientific™ Megafuge™ 8 Centrifuge .................................... 7
Thermo Scientific™ Megafuge™ 8R Centrifuge ................................. 8
Thermo Scientific™ Sorvall™ ST 8 Centrifuge ................................... 9
Thermo Scientific™ Sorvall™ ST 8R Centrifuge .................................10
Thermo Scientific SL 8 Centrifuge ........................................... 11
Thermo Scientific SL 8R Centrifuge .......................................... 12
Accessories ......................................................13
Thermo Scientific
Auto-Lock Rotor Exchange ..........................................15
Rotor Installation ........................................................ 15
Removing the Rotor ...................................................... 16
Rotor Loading .....................................................17
Before a Run ........................................................... 17
Filling the Hematocrit Capillaries ............................................ 17
Rotor Temperature Range ................................................. 18
Duration .............................................................. 18
Using the Reading Graph .................................................. 19
Rotor Life Time ......................................................... 20
Maintenance and Care ..............................................21
Cleaning Intervals ....................................................... 21
Replace Sealing ........................................................ 21
How to deal with broken Hematocrit Capillary Tubes ............................. 22
Basics ................................................................ 22
Cleaning .............................................................. 23
Disinfection ............................................................ 24
Decontamination ........................................................ 25
Table of Contents
Table of Contents
4 | Hematocrit Rotor Thermo Scientific
Autoclaving ............................................................26
Service of Thermo Fisher Scientific ..........................................26
Storage ............................................................... 26
Shipping and Disposal .................................................... 26
RCF-Values .......................................................27
Chemical Compatibility Chart ........................................31
Index ............................................................37
Thermo Scientific Hematocrit Rotor | 5
Before starting to use the rotor, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
warranty.
Items Supplied
Article No. Item Quantity
75005733 Hematocrit Rotor 1
70009284 Anti-corrosion Oil 1
50139838 Rubber Band White NBR, Shore 65 3
76000938 Reading Graph 1
50139640 Instruction Manual 1
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Intended Use
Together with compatible centrifuges this hematocrit rotor (75005733) is intended to be used as an in-vitro diagnostics
device to quantitatively determine the hematocrit levels in blood samples.
This applies only if the Hematocrit Rotor is used with approved accessories and items listed in the items supplied (see
“Items Supplied” on page 5) and accessories (see “Accessories” on page 13) sections.
This rotor and its accessories should be operated by trained specialists only.
Preface
P
Precautions
6 | Hematocrit Rotor Thermo Scientific
Precautions
In order to ensure safe operation of the Hematocrit Rotor, the following general safety regulations must be followed:
• Observe the safety instructions.
• Do not remove the magnets at the rotor bottom.
• Do not use rotors which show any signs of corrosion and/or cracks. Do not touch the electronic components of the
centrifuge and do not make any changes to the electronic or mechanical components.
• Use only a properly loaded rotor.
• Never overload the rotor.
• Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this rule are
commercially available glass or plastic centrifuge tubes, that are approved for the speed or the RCF value of the
rotor.
• Check that the rotor is locked properly into place before operating the centrifuge.
CAUTION
Due to the air friction the temperature of rotor may raise significantly while the centrifuge is spinning. Ventilated units
will always lead to temperature increase compared to ambient temperatures. Refrigerated units have limitations in
cooling capabilities. Displayed and set temperature can deviate from sample temperature. Sample temperature might
exceed critical temperature of your application.
WARNING
The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers.
The magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent
magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no
interference.
Symbols used in the manual
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
Thermo Scientific Hematocrit Rotor | 7
Rotor Specifications
Thermo Scientific™ Megafuge™ 8 Centrifuge
Frequency 230 V 120 V
Weight (empty) 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g
K-Value at nmax 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90°
Acceleration / Braking Time 14 s / 24 s 14 s / 24 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C
Aerosol-tight No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C
1
Chapter 1 | Thermo Scientific™ Megafuge™ 8R Centrifuge
8 | Hematocrit Rotor Thermo Scientific
Thermo Scientific™ Megafuge™ 8R Centrifuge
Frequency 230 V 120 V
Weight (empty) 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g
K-Value at nmax 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90°
Acceleration / Braking Time 20 s / 25 s 15 s / 30 s
Maximum Speed at 4 °C 13300 rpm 13300 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C
Aerosol-tight No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C
Thermo Scientific™ Sorvall™ ST 8 Centrifuge | Chapter 1
Thermo Scientific Hematocrit Rotor | 9
Thermo Scientific™ Sorvall™ ST 8 Centrifuge
Frequency 230 V 120 V 100 V
Weight (empty) 1.0 kg 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g 16810 x g
K-Value at nmax 2069 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90° 90°
Acceleration / Braking Time 14 s / 24 s 14 s / 24 s 14 s / 24 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C 16 °C
Aerosol-tight No No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C 134 °C
Chapter 1 | Thermo Scientific™ Sorvall™ ST 8R Centrifuge
10 | Hematocrit Rotor Thermo Scientific
Thermo Scientific™ Sorvall™ ST 8R Centrifuge
Frequency 230 V 120 V 100 V
Weight (empty) 1.0 kg 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g 16810 x g
K-Value at nmax 2069 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90° 90°
Acceleration / Braking Time 20 s / 25 s 15 s / 30 s 15 s / 30 s
Maximum Speed at 4 °C 13300 rpm 13300 rpm 13300 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C < 4 °C
Aerosol-tight No No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C 134 °C
Thermo Scientific SL 8 Centrifuge | Chapter 1
Thermo Scientific Hematocrit Rotor | 11
Thermo Scientific SL 8 Centrifuge
Frequency 230 V 120 V
Weight (empty) 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g
K-Value at nmax 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90°
Acceleration / Braking Time 14 s / 24 s 14 s / 24 s
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C
Aerosol-tight No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C
Chapter 1 | Thermo Scientific SL 8R Centrifuge
12 | Hematocrit Rotor Thermo Scientific
Thermo Scientific SL 8R Centrifuge
Frequency 230 V 120 V
Weight (empty) 1.0 kg 1.0 kg
Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g
Maximum Speed nmax 13300 rpm 13300 rpm
Maximum RCF-Value at nmax 16810 x g 16810 x g
K-Value at nmax 2069 2069
Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm
Angle 90° 90°
Acceleration / Braking Time 20 s / 25 s 15 s / 30 s
Maximum Speed at 4 °C 13300 rpm 13300 rpm
Sample Temperature at nmax
(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C
Aerosol-tight No No
Permissible Temperature Range
Autoclavable 134 °C 134 °C
Thermo Scientific Hematocrit Rotor | 13
Accessories
Article No.
Capillaries 76000923
Sealing Kit 75000964
Replacement Rubber Bands (Set of 5) 75003030
Replacement Reading Graph 76000938
22
Chapter 2 |
14 | Hematocrit Rotor Thermo Scientific
Thermo Scientific Hematocrit Rotor | 15
Thermo Scientic
Auto-Lock Rotor Exchange
Rotor Installation
CAUTION
Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge.
This rotor is equipped with a Thermo Scientific™ Auto-Lock™ rotor exchange.
This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted onto
the centrifuge spindle.
Proceed as follows:
1. Open the door of the centrifuge and if necessary remove any dust, foreign objects or residue from the chamber.
Auto-Lock and o-ring must be clean and undamaged.
1
2
1Auto-Lock
2O-Ring
2. Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle. The rotor clicks
automatically into place.
CAUTION
Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with little pressure.
33
Chapter 3 | Removing the Rotor
16 | Hematocrit Rotor Thermo Scientific
3. Check if the rotor is properly installed by lifting it slightly on the handle. If the rotor can be pulled up, then it must
be re-clamped to the centrifuge spindle.
WARNING
If the rotor cannot be properly locked in place after several attempts, then the Auto-Lock is defective and you are not
permitted to operate the rotor.
Check for any damage to the rotor: Damaged rotors must not be used.
Keep the centrifuge spindle area of the rotor clear of objects.
If in doubt please call Thermo Fisher Scientific Customer Service.
CAUTION
Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle or from
beneath the rotor. The rotor has to be locked tight.
4. Close the centrifuge door.
Removing the Rotor
To remove the rotor, proceed as follows:
1. Open the centrifuge door.
2. Grab the rotor handle with one or both hands and push down the Auto-Lock button. At the same time, pull the rotor
directly upwards and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this.
Thermo Scientific Hematocrit Rotor | 17
Before a Run
1. Read and observe the precautions and safety instructions in this instruction manual and in the instruction manual
of the centrifuge.
2. Check the rotor and all accessory parts for damages such as cracks, scratches or traces of corrosion.
3. Check the rotor chamber, the centrifuge spindle and the Auto-Lock.
4. Check the rotor suitability. see “Rotor Loading” on page 17.
Filling the Hematocrit Capillaries
WARNING
Loss of sample, incorrect results, risk of cuts and infections result through the breakage of glass.
Handle the hematocrit capillaries with care and if necessary use protective equipment.
Only use the listed hematocrit capillaries (76000923, see “Accessories” on page 13).
CAUTION
Wrong results will be generated if capillary is filled with pre-separated fraction of blood. Before filling the capillaries
shake up the blood sample.
NOTICE
Capillaries are for single use. They are to be disposed after being used. Follow regulations for proper disposal.
1. Shake up the blood sample before filling the capillary.
2. Hold the capillary slanted with one end into the blood sample.
3. Fill the hematocrit capillary (76000923) with a blood column of approximately 65 mm.
Make sure that the second opening remains dry.
4. Close the dry end of the hematocrit capillary with sealing putty (75000964). To do this, push the hematocrit capillary
vertically into the sealing putty until its rim touches the sealing putty’s plate.
Tilt it slightly and pull the hematocrit capillary carefully out of the putty. Make sure that the capillaries are well sealed with
the sealing putty.
Rotor Loading
4
Chapter 4 | Rotor Temperature Range
18 | Hematocrit Rotor Thermo Scientific
Rotor Temperature Range
CAUTION
Operate the rotor in a temperature range between 4 °C and 40 °C only.
A pre-tempering in a freezer below 4 °C is not allowed.
NOTICE
The rotor can warm up in ventilated centrifuges. At temperatures above 45 °C the blood samples can be damaged.
Let the rotor cool down between two runs.
Duration
The duration depends on the RCF-value. For a clear separation the run should last at least 5 minutes. The following values
are suggested for reproducible results.
Speed rpm Duration in minutes
13300 8
Explanation of RCF-Value
The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unit less numerical value which is
used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of
device. Only the centrifuging radius and the speed are used for its calculation:
RCF = 11.18 x x r
r = centrifuging radius in cm
n = rotational speed in rpm
The maximum RCF value is related to the maximum radius of the tube opening.
Remember that this value is reduced depending on the tubes and adapters used.
This can be accounted for in the calculation above if required.
Rotor Loading
1. Place the hematocrit capillary with its closed end pointing outwards (towards the rim of the rotor) horizontally into
the hematocrit rotor. The hematocrit capillary must touch the sealing (50139838). The sealing protects the sensible
capillaries from the hard rotor rim. The capillaries can break during a run when the sealing is missing or pierce the
sealing after excessive reuse without changing or shifting.
CAUTION
Ensure that a balancing hematocrit capillary is placed opposite in order to avoid imbalance and possible damage.
2. Close the rotor lid by turning the handle clockwise.
CAUTION
Damaged parts must be replaced immediately.
Using the Reading Graph |
Thermo Scientific Hematocrit Rotor | 19
Using the Reading Graph
The reading graph (76000938) is supplied with the rotor. To read the values, proceed as follows:
1. Remove the hematocrit capillaries one by one from the rotor.
NOTICE
Be careful not to shake up the sample while removing. If the samples have been shaken up during removal, they can
be centrifuged again.
2. Place the bottom end of the blood column on the zero line with the top end of the plasma column on the 100 %
line of the graph.
The dividing line between the Buffy coat and the erythrocytes sediment indicates the percentage of packed cells.
1
4
7
8
5
3
2
6
1Linear scale reading graph
2Zero line
3Sealing putty
4Erythrocytes
5Dividing line between erythrocytes and buffy coat
6Buffy coat (leukocoytes and platelets)
7Plasma
8Air
NOTICE
Be careful to place the hematocrit capillary at a right angle to the zero line.
Chapter 4 | Rotor Life Time
20 | Hematocrit Rotor Thermo Scientific
Rotor Life Time
The rotor has no life-time restrictions. For safety reasons please bear the following in mind:
• UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct
sunlight.
• If the rotor shows signs of corrosion, decoloration, deformation, wear or imbalance it must be replaced.
• At the advised parameters for centrifugation of maximum speed 13300 rpm for 8 minutes the rubber band will last
for at least 30 cycles before it has to be replaced or shifted.
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