Thermo Fisher Scientific Hematocrit Rotor User manual

Brand: Thermo Fisher Scientific

Model: Hematocrit Rotor

Type: User manual

Thermo Scientiﬁc

Hematocrit Rotor

for Thermo Scientiﬁc Megafuge 8 / 8R,

Sorvall ST 8 / 8R and SL 8 / 8 R Centrifuges

Instruction Manual

50143238-e • 06 / 2022

Visit us online to register your warranty:

thermofisher.com/labwarranty

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment

(WEEE) Directive 2012/19/EU. It is marked with the following symbol:

Thermo Scientiﬁc Hematocrit Rotor | 3

WEEE Compliance ...................................................2

Table of Contents ...................................................3

Preface ...........................................................5

Items Supplied .......................................................... 5

Intended Use ............................................................ 5

Precautions ............................................................. 6

Symbols used in the manual ................................................ 6

Rotor Specifications ................................................. 7

Thermo Scientific™ Megafuge™ 8 Centrifuge .................................... 7

Thermo Scientific™ Megafuge™ 8R Centrifuge ................................. 8

Thermo Scientific™ Sorvall™ ST 8 Centrifuge ................................... 9

Thermo Scientific™ Sorvall™ ST 8R Centrifuge .................................10

Thermo Scientific SL 8 Centrifuge ........................................... 11

Thermo Scientific SL 8R Centrifuge .......................................... 12

Accessories ......................................................13

Thermo Scientific

Auto-Lock Rotor Exchange ..........................................15

Rotor Installation ........................................................ 15

Removing the Rotor ...................................................... 16

Rotor Loading .....................................................17

Before a Run ........................................................... 17

Filling the Hematocrit Capillaries ............................................ 17

Rotor Temperature Range ................................................. 18

Duration .............................................................. 18

Using the Reading Graph .................................................. 19

Rotor Life Time ......................................................... 20

Maintenance and Care ..............................................21

Cleaning Intervals ....................................................... 21

Replace Sealing ........................................................ 21

How to deal with broken Hematocrit Capillary Tubes ............................. 22

Basics ................................................................ 22

Cleaning .............................................................. 23

Disinfection ............................................................ 24

Decontamination ........................................................ 25

Table of Contents

4 | Hematocrit Rotor Thermo Scientiﬁc

Autoclaving ............................................................26

Service of Thermo Fisher Scientific ..........................................26

Storage ............................................................... 26

Shipping and Disposal .................................................... 26

RCF-Values .......................................................27

Chemical Compatibility Chart ........................................31

Index ............................................................37

Thermo Scientiﬁc Hematocrit Rotor | 5

Before starting to use the rotor, read through this instruction manual carefully and follow the instructions.

Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers

warranty.

Items Supplied

Article No. Item Quantity

75005733 Hematocrit Rotor 1

70009284 Anti-corrosion Oil 1

50139838 Rubber Band White NBR, Shore 65 3

76000938 Reading Graph 1

50139640 Instruction Manual 1

If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Intended Use

Together with compatible centrifuges this hematocrit rotor (75005733) is intended to be used as an in-vitro diagnostics

device to quantitatively determine the hematocrit levels in blood samples.

This applies only if the Hematocrit Rotor is used with approved accessories and items listed in the items supplied (see

“Items Supplied” on page 5) and accessories (see “Accessories” on page 13) sections.

This rotor and its accessories should be operated by trained specialists only.

Preface

Precautions

6 | Hematocrit Rotor Thermo Scientiﬁc

Precautions

In order to ensure safe operation of the Hematocrit Rotor, the following general safety regulations must be followed:

• Observe the safety instructions.

• Do not remove the magnets at the rotor bottom.

• Do not use rotors which show any signs of corrosion and/or cracks. Do not touch the electronic components of the

centrifuge and do not make any changes to the electronic or mechanical components.

• Use only a properly loaded rotor.

• Never overload the rotor.

• Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this rule are

commercially available glass or plastic centrifuge tubes, that are approved for the speed or the RCF value of the

rotor.

• Check that the rotor is locked properly into place before operating the centrifuge.

CAUTION

Due to the air friction the temperature of rotor may raise signiﬁcantly while the centrifuge is spinning. Ventilated units

will always lead to temperature increase compared to ambient temperatures. Refrigerated units have limitations in

cooling capabilities. Displayed and set temperature can deviate from sample temperature. Sample temperature might

exceed critical temperature of your application.

WARNING

The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers.

The magnets are mounted on the bottom of the rotor.

Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent

magnetic ﬁelds. The magnetic ﬁeld strength at a distance of 20 cm is less than 0.1 mT, so there should be no

interference.

Symbols used in the manual

This symbol refers to general hazards.

CAUTION means that material damage could occur.

WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your

environment safe.

Thermo Scientiﬁc Hematocrit Rotor | 7

Rotor Speciﬁcations

Thermo Scientiﬁc™ Megafuge™ 8 Centrifuge

Frequency 230 V 120 V

Weight (empty) 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g

K-Value at nmax 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90°

Acceleration / Braking Time 14 s / 24 s 14 s / 24 s

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C

Aerosol-tight No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C

Chapter 1 | Thermo Scientiﬁc™ Megafuge™ 8R Centrifuge

8 | Hematocrit Rotor Thermo Scientiﬁc

Thermo Scientiﬁc™ Megafuge™ 8R Centrifuge

Frequency 230 V 120 V

Weight (empty) 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g

K-Value at nmax 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90°

Acceleration / Braking Time 20 s / 25 s 15 s / 30 s

Maximum Speed at 4 °C 13300 rpm 13300 rpm

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C

Aerosol-tight No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C

Thermo Scientiﬁc™ Sorvall™ ST 8 Centrifuge | Chapter 1

Thermo Scientiﬁc Hematocrit Rotor | 9

Thermo Scientiﬁc™ Sorvall™ ST 8 Centrifuge

Frequency 230 V 120 V 100 V

Weight (empty) 1.0 kg 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g 16810 x g

K-Value at nmax 2069 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90° 90°

Acceleration / Braking Time 14 s / 24 s 14 s / 24 s 14 s / 24 s

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C 16 °C

Aerosol-tight No No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C 134 °C

Chapter 1 | Thermo Scientiﬁc™ Sorvall™ ST 8R Centrifuge

10 | Hematocrit Rotor Thermo Scientiﬁc

Thermo Scientiﬁc™ Sorvall™ ST 8R Centrifuge

Frequency 230 V 120 V 100 V

Weight (empty) 1.0 kg 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g 16810 x g

K-Value at nmax 2069 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90° 90°

Acceleration / Braking Time 20 s / 25 s 15 s / 30 s 15 s / 30 s

Maximum Speed at 4 °C 13300 rpm 13300 rpm 13300 rpm

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C < 4 °C

Aerosol-tight No No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C 134 °C

Thermo Scientiﬁc SL 8 Centrifuge | Chapter 1

Thermo Scientiﬁc Hematocrit Rotor | 11

Thermo Scientiﬁc SL 8 Centrifuge

Frequency 230 V 120 V

Weight (empty) 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g

K-Value at nmax 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90°

Acceleration / Braking Time 14 s / 24 s 14 s / 24 s

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) 16 °C 16 °C

Aerosol-tight No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C

Chapter 1 | Thermo Scientiﬁc SL 8R Centrifuge

12 | Hematocrit Rotor Thermo Scientiﬁc

Thermo Scientiﬁc SL 8R Centrifuge

Frequency 230 V 120 V

Weight (empty) 1.0 kg 1.0 kg

Maximum Permissible Load 24 x 0.2 g 24 x 0.2 g

Maximum Speed nmax 13300 rpm 13300 rpm

Maximum RCF-Value at nmax 16810 x g 16810 x g

K-Value at nmax 2069 2069

Radius max. / min. 85 mm / 20 mm 85 mm / 20 mm

Angle 90° 90°

Acceleration / Braking Time 20 s / 25 s 15 s / 30 s

Maximum Speed at 4 °C 13300 rpm 13300 rpm

Sample Temperature at nmax

(Ambient temperature of 23 °C, run time 60 minutes) < 4 °C < 4 °C

Aerosol-tight No No

Permissible Temperature Range

Autoclavable 134 °C 134 °C

Thermo Scientiﬁc Hematocrit Rotor | 13

Accessories

Article No.

Capillaries 76000923

Sealing Kit 75000964

Replacement Rubber Bands (Set of 5) 75003030

Replacement Reading Graph 76000938

Chapter 2 |

14 | Hematocrit Rotor Thermo Scientiﬁc

Thermo Scientiﬁc Hematocrit Rotor | 15

Thermo Scientic

Auto-Lock Rotor Exchange

Rotor Installation

CAUTION

Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge.

This rotor is equipped with a Thermo Scientific™ Auto-Lock™ rotor exchange.

This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted onto

the centrifuge spindle.

Proceed as follows:

1. Open the door of the centrifuge and if necessary remove any dust, foreign objects or residue from the chamber.

Auto-Lock and o-ring must be clean and undamaged.

1Auto-Lock

2O-Ring

2. Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle. The rotor clicks

automatically into place.

CAUTION

Do not force the rotor onto the centrifuge spindle.

If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with little pressure.

Chapter 3 | Removing the Rotor

16 | Hematocrit Rotor Thermo Scientiﬁc

3. Check if the rotor is properly installed by lifting it slightly on the handle. If the rotor can be pulled up, then it must

be re-clamped to the centrifuge spindle.

WARNING

If the rotor cannot be properly locked in place after several attempts, then the Auto-Lock is defective and you are not

permitted to operate the rotor.

Check for any damage to the rotor: Damaged rotors must not be used.

Keep the centrifuge spindle area of the rotor clear of objects.

If in doubt please call Thermo Fisher Scientiﬁc Customer Service.

CAUTION

Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle or from

beneath the rotor. The rotor has to be locked tight.

4. Close the centrifuge door.

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Grab the rotor handle with one or both hands and push down the Auto-Lock button. At the same time, pull the rotor

directly upwards and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this.

Thermo Scientiﬁc Hematocrit Rotor | 17

Before a Run

1. Read and observe the precautions and safety instructions in this instruction manual and in the instruction manual

of the centrifuge.

2. Check the rotor and all accessory parts for damages such as cracks, scratches or traces of corrosion.

3. Check the rotor chamber, the centrifuge spindle and the Auto-Lock.

4. Check the rotor suitability. see “Rotor Loading” on page 17.

Filling the Hematocrit Capillaries

WARNING

Loss of sample, incorrect results, risk of cuts and infections result through the breakage of glass.

Handle the hematocrit capillaries with care and if necessary use protective equipment.

Only use the listed hematocrit capillaries (76000923, see “Accessories” on page 13).

CAUTION

Wrong results will be generated if capillary is ﬁlled with pre-separated fraction of blood. Before ﬁlling the capillaries

shake up the blood sample.

NOTICE

Capillaries are for single use. They are to be disposed after being used. Follow regulations for proper disposal.

1. Shake up the blood sample before filling the capillary.

2. Hold the capillary slanted with one end into the blood sample.

3. Fill the hematocrit capillary (76000923) with a blood column of approximately 65 mm.

Make sure that the second opening remains dry.

4. Close the dry end of the hematocrit capillary with sealing putty (75000964). To do this, push the hematocrit capillary

vertically into the sealing putty until its rim touches the sealing putty’s plate.

Tilt it slightly and pull the hematocrit capillary carefully out of the putty. Make sure that the capillaries are well sealed with

the sealing putty.

Rotor Loading

Chapter 4 | Rotor Temperature Range

18 | Hematocrit Rotor Thermo Scientiﬁc

Rotor Temperature Range

CAUTION

Operate the rotor in a temperature range between 4 °C and 40 °C only.

A pre-tempering in a freezer below 4 °C is not allowed.

NOTICE

The rotor can warm up in ventilated centrifuges. At temperatures above 45 °C the blood samples can be damaged.

Let the rotor cool down between two runs.

Duration

The duration depends on the RCF-value. For a clear separation the run should last at least 5 minutes. The following values

are suggested for reproducible results.

Speed rpm Duration in minutes

13300 8

Explanation of RCF-Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unit less numerical value which is

used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of

device. Only the centrifuging radius and the speed are used for its calculation:

RCF = 11.18 x x r

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Remember that this value is reduced depending on the tubes and adapters used.

This can be accounted for in the calculation above if required.

Rotor Loading

1. Place the hematocrit capillary with its closed end pointing outwards (towards the rim of the rotor) horizontally into

the hematocrit rotor. The hematocrit capillary must touch the sealing (50139838). The sealing protects the sensible

capillaries from the hard rotor rim. The capillaries can break during a run when the sealing is missing or pierce the

sealing after excessive reuse without changing or shifting.

CAUTION

Ensure that a balancing hematocrit capillary is placed opposite in order to avoid imbalance and possible damage.

2. Close the rotor lid by turning the handle clockwise.

CAUTION

Damaged parts must be replaced immediately.

Using the Reading Graph |

Thermo Scientiﬁc Hematocrit Rotor | 19

Using the Reading Graph

The reading graph (76000938) is supplied with the rotor. To read the values, proceed as follows:

1. Remove the hematocrit capillaries one by one from the rotor.

NOTICE

Be careful not to shake up the sample while removing. If the samples have been shaken up during removal, they can

be centrifuged again.

2. Place the bottom end of the blood column on the zero line with the top end of the plasma column on the 100 %

line of the graph.

The dividing line between the Buffy coat and the erythrocytes sediment indicates the percentage of packed cells.

1Linear scale reading graph

2Zero line

3Sealing putty

4Erythrocytes

5Dividing line between erythrocytes and buffy coat

6Buffy coat (leukocoytes and platelets)

7Plasma

8Air

NOTICE

Be careful to place the hematocrit capillary at a right angle to the zero line.

Chapter 4 | Rotor Life Time

20 | Hematocrit Rotor Thermo Scientiﬁc

Rotor Life Time

The rotor has no life-time restrictions. For safety reasons please bear the following in mind:

• UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct

sunlight.

• If the rotor shows signs of corrosion, decoloration, deformation, wear or imbalance it must be replaced.

• At the advised parameters for centrifugation of maximum speed 13300 rpm for 8 minutes the rubber band will last

for at least 30 cycles before it has to be replaced or shifted.

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Thermo Fisher Scientific Hematocrit Rotor User manual

Brand: Thermo Fisher Scientific

Model: Hematocrit Rotor

Type: User manual

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Thermo Fisher Scientific Hematocrit Rotor User manual

Thermo Fisher Scientific Hematocrit Rotor User manual

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