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Fluigent Droplet User guide

  • Hello! I've reviewed the Quick Start Guide for the FLUIGENT Droplet Kit, which includes the EZ Drop chip, Flow EZ, Flow Unit and P-Cap. This document provides instructions on how to set up and use the device for generating water-in-oil droplets using flow focusing. I am ready to answer any questions you might have about the setup process, connection tips, and operational features described in the guide.
  • What is the EZ Drop chip used for?
    How many devices are on each EZ Drop chip?
    What size tubing is used for connections?
    How is droplet size and generation rate controlled?
FluigentDropletStarterPack
QuickStartGuide
Contentinthispack
1DropletKit
3EZDropchip
1/32"tubing
2Adaptorsleeve
2FlowEZTM
1FlowEZTMsupplykit
2FlowUnit
2Lowflowratekit
2PCap
2PCapkit
1Tubecutter
ThisguidemayalsobeusedbyDroletKituserforinformationpurpose.
Datasheetisavailableathttps://www.fluigent.com/product/dropletstarterpack/
Foranyquestion:contact@fluigent.com/www.fluigent.com
Chipoverview
TheEZDropisamicrofluidicchiptogeneratewaterinoildroplets(usingflowfocusing).Each
chipcontains3identicaldevicesandeachonecanbeusedindependently.
Channeloverviewofasingledevice
Oilinlet Waterinlet Outlet
Nozzlezone(Seeright) Nozzlezone
Scaleof20µm,50µmand100µm
(foraquickdroplets'sizeidentificationundermicroscope)
Setupinstallation
PoweronyourFlowEZsandfollowthefirsttimeuseprocedure(SeeFlowEZ's
quickstartguide).
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2
Installthewholesetupasshownbelow(Useoffilteredsolutionswillenhancechiplife).
Aqueoussolution/
Sample
Oil(filtered<0.45µm)
Preparation
"Water"FlowEZ "Oil"FlowEZ
1/32''fluidic
tubing
Cable(notshown)
connectedtoits
relatedFlowEZ
"Oil"and"Water"channel
(tobeconnectedintothechip)
supplied
pressure
Forexemple:
MineralOil(SigmaRefM8410)
with2%w/wSpan80(SigmaRef85548)
2
connected
to"Water"
channel
connected
to"Oil"
channel todropletcollection
1
Makesurethe1/32"tubingyouareabouttoinserthasastraightcleancutatitsend.If
notso,useatubecuttertomakeastraightcleancut.
2
3
~1mm
Repeatpreviousstepsforthetwootherconnectionholesonthesamedevice.
(lateralview,channelsizenotinscale)
Chipconnectiontips
straightcleancut
4
Insertthetubinggentlyuntilittouchesthebottom.
Pullthetubingupgentlyforabout1mm(toleavespacefortheflow).
exampleofthe"Oil"channeltubing
insertedintothe"Oil"connectionhole
3
Orange:Oilflowrate
Green:Waterflowrate
unit:µL/min
Generatingdroplets
1
2
3
Setthepressureto2000mbaronthe"Oil"FlowEZand1000mbaronthe"Water"FlowEZ.
Waitafewsecondsuntilyouobservewaterjettinginthenozzlezoneunderthemicroscope.
waterjetting
oil
Reducethethepressureinthe"Water"channel(byturningthedialonthe"Water"FlowEZ),
untilyouobserveastabledropletgeneration.
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Ifyouneedtoachieveaspecificsizeandrateofdroplets,adjustthepressurestoreacha
certainOil/Waterflowratecombination(flowrateisshownasQmeasonyourFlowEZ'sscreen).
YoucanusethediagrambelowtoquicklyidentifytheOil/Waterflowratecombinationforthe
desireddiameterandgeneratingrate.
Droplets'rate/sizediagram(part)
Exampleofuse:Foracombinationof1.0µL/minofoiland1.0µL/minofwater,youmayachieveadropletdiameterof55µmatarateof190Hz
(11400dropletsperminute).
Onlythepartunder1.0µL/minispresentedhere
Thisdiagramshowsonlythepartoflowflowrate.TheEZDropchipitselfcanachieveupto4000Hz
undercertaincircumstances(Seethedatasheetformoredetails).
Resultsmaybedifferentfromdataonthisdiagram,dependingonexperimentalenvironment.
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