Fluigent Droplet User guide

Brand
Fluigent
Model
Droplet
Type
User guide

Fluigent Droplet is a microfluidic chip designed to generate water-in-oil droplets using a technique called flow focusing. The device consists of three identical microfluidic devices, each with water and oil inlets and an outlet. By controlling the flow rates of the two fluids, users can generate droplets of various sizes and rates. Applications include cell encapsulation, drug delivery, and chemical synthesis. With its ability to precisely control droplet size and generation rate, Fluigent Droplet is a versatile tool for a wide range of research and industrial applications.

Fluigent Droplet is a microfluidic chip designed to generate water-in-oil droplets using a technique called flow focusing. The device consists of three identical microfluidic devices, each with water and oil inlets and an outlet. By controlling the flow rates of the two fluids, users can generate droplets of various sizes and rates. Applications include cell encapsulation, drug delivery, and chemical synthesis. With its ability to precisely control droplet size and generation rate, Fluigent Droplet is a versatile tool for a wide range of research and industrial applications.

FluigentDropletStarterPack
QuickStartGuide
Contentinthispack
1DropletKit
3EZDropchip
1/32"tubing
2Adaptorsleeve
2FlowEZTM
1FlowEZTMsupplykit
2FlowUnit
2Lowflowratekit
2PCap
2PCapkit
1Tubecutter
ThisguidemayalsobeusedbyDroletKituserforinformationpurpose.
Datasheetisavailableathttps://www.fluigent.com/product/dropletstarterpack/
Foranyquestion:contact@fluigent.com/www.fluigent.com
Chipoverview
TheEZDropisamicrofluidicchiptogeneratewaterinoildroplets(usingflowfocusing).Each
chipcontains3identicaldevicesandeachonecanbeusedindependently.
Channeloverviewofasingledevice
Oilinlet Waterinlet Outlet
Nozzlezone(Seeright) Nozzlezone
Scaleof20µm,50µmand100µm
(foraquickdroplets'sizeidentificationundermicroscope)
Setupinstallation
PoweronyourFlowEZsandfollowthefirsttimeuseprocedure(SeeFlowEZ's
quickstartguide).
1
2
Installthewholesetupasshownbelow(Useoffilteredsolutionswillenhancechiplife).
Aqueoussolution/
Sample
Oil(filtered<0.45µm)
Preparation
"Water"FlowEZ "Oil"FlowEZ
1/32''fluidic
tubing
Cable(notshown)
connectedtoits
relatedFlowEZ
"Oil"and"Water"channel
(tobeconnectedintothechip)
supplied
pressure
Forexemple:
MineralOil(SigmaRefM8410)
with2%w/wSpan80(SigmaRef85548)
2
connected
to"Water"
channel
connected
to"Oil"
channel todropletcollection
1
Makesurethe1/32"tubingyouareabouttoinserthasastraightcleancutatitsend.If
notso,useatubecuttertomakeastraightcleancut.
2
3
~1mm
Repeatpreviousstepsforthetwootherconnectionholesonthesamedevice.
(lateralview,channelsizenotinscale)
Chipconnectiontips
straightcleancut
4
Insertthetubinggentlyuntilittouchesthebottom.
Pullthetubingupgentlyforabout1mm(toleavespacefortheflow).
exampleofthe"Oil"channeltubing
insertedintothe"Oil"connectionhole
3
Orange:Oilflowrate
Green:Waterflowrate
unit:µL/min
Generatingdroplets
1
2
3
Setthepressureto2000mbaronthe"Oil"FlowEZand1000mbaronthe"Water"FlowEZ.
Waitafewsecondsuntilyouobservewaterjettinginthenozzlezoneunderthemicroscope.
waterjetting
oil
Reducethethepressureinthe"Water"channel(byturningthedialonthe"Water"FlowEZ),
untilyouobserveastabledropletgeneration.
4
Ifyouneedtoachieveaspecificsizeandrateofdroplets,adjustthepressurestoreacha
certainOil/Waterflowratecombination(flowrateisshownasQmeasonyourFlowEZ'sscreen).
YoucanusethediagrambelowtoquicklyidentifytheOil/Waterflowratecombinationforthe
desireddiameterandgeneratingrate.
Droplets'rate/sizediagram(part)
Exampleofuse:Foracombinationof1.0µL/minofoiland1.0µL/minofwater,youmayachieveadropletdiameterof55µmatarateof190Hz
(11400dropletsperminute).
Onlythepartunder1.0µL/minispresentedhere
Thisdiagramshowsonlythepartoflowflowrate.TheEZDropchipitselfcanachieveupto4000Hz
undercertaincircumstances(Seethedatasheetformoredetails).
Resultsmaybedifferentfromdataonthisdiagram,dependingonexperimentalenvironment.
4
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Fluigent Droplet User guide

Brand
Fluigent
Model
Droplet
Type
User guide

Fluigent Droplet is a microfluidic chip designed to generate water-in-oil droplets using a technique called flow focusing. The device consists of three identical microfluidic devices, each with water and oil inlets and an outlet. By controlling the flow rates of the two fluids, users can generate droplets of various sizes and rates. Applications include cell encapsulation, drug delivery, and chemical synthesis. With its ability to precisely control droplet size and generation rate, Fluigent Droplet is a versatile tool for a wide range of research and industrial applications.

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