Thermo Fisher Scientific T-647.5 Fixed Angle Rotor User manual

Brand: Thermo Fisher Scientific

Model: T-647.5 Fixed Angle Rotor

Category: Fridge-freezers

Type: User manual

8326-10 March 2010

Thermo Fisher Scientific

T-647.5

Instruction Manual

ULTRACRIMP< is either a registered trademark or a trademark of Thermo Fisher Scientific.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might

result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: 8326-10 printed in March 2010.

For Research Use Only. Not for use in diagnostic procedures.

Thermo Scientific T-647.5 i

Thermo Scientific T-647.5 Titanium Fixed-Angle Ultra-speed Centrifuge Rotor .......... ii

Important Safety Information ................................................................................................. iii

Chapter 1 DESCRIPTION ........................................................................................................................ 1-1

Rotor Description .................................................................................................... 1-2

Rotor Specifications ................................................................................................. 1-2

Accessories ............................................................................................................... 1-3

Chapter 2 OPERATION ............................................................................................................................ 2-1

Prerun Safety Checks ............................................................................................... 2-2

Compartment Loads in Excess of Design Mass ........................................................ 2-2

Critical Speed .......................................................................................................... 2-3

Rotor Precool .......................................................................................................... 2-3

Relative Centrifugal Force (RCF) Determination .................................................... 2-3

Chemical Compatibility .......................................................................................... 2-7

Rotor Balancing ....................................................................................................... 2-7

Rotor Loading and Sealing ....................................................................................... 2-8

Ultracentrifuge / Rotor Log Book ............................................................................ 2-9

Chapter 3 CARE and MAINTENANCE ................................................................................................... 3-1

Corrosion ................................................................................................................ 3-2

Cleaning .................................................................................................................. 3-2

Storage ..................................................................................................................... 3-4

Overspeed Decal Replacement ................................................................................. 3-4

Service Decontamination Policy .............................................................................. 3-5

Chemical Compatibility Chart ...............................................................................A-1

Contact Information ................................................................................................B-1

Table of contents

Thermo Scientific T-647.5 ii

This manual is a guide for the use of

Thermo Scientific T-647.5 Titanium Fixed-Angle

Ultra-speed Centrifuge Rotor

Data herein has been verified and is believed adequate for the intended use of the rotor. Because failure

to follow the recommendations set forth in this manual could produce personal injury or property

damage, always follow the recommendations set forth herein. Thermo Fisher Scientific does not

guarantee results and assumes no obligation for the performance of rotors or other products that are

not used in accordance with the instructions provided. This publication is not a license to operate

under, nor a recommendation to infringe upon, any process patents.

Publications prior to the Issue Date of this manual may contain data in apparent conflict with that

provided herein. Please consider all data in this manual to be the most current.

NOTES, CAUTIONS, and WARNINGS within the text of this manual are used to emphasize

important and critical instructions.

WARNING informs the operator of a hazard or unsafe practice that could result in personal injury,

affect the operator's health, or contaminate the environment.

CAUTION informs the operator of an unsafe practice that could result in damage of equipment.

NOTE highlights essential information.

CAUTION and WARNING are accompanied by a hazard symbol and appear near the

information they correspond to.

Preface

iii T-647.5 Thermo Scientific

Important Safety Information

Certain potentially dangerous conditions are inherent to the use of all centrifuge rotors. To ensure safe

operation of this rotor, anyone using it should be aware of all safe practices and take all precautions

described below and throughout this manual.

WARNING

When using radioactive, toxic, or pathogenic materials, be aware of all characteristics of

the materials and the hazards associated with them in the event leakage occurs during

centrifugation. In the event of a rotor failure, neither the centrifuge nor the rotor can

protect you from particles dispersed in the air. To protect yourself, we recommend

additional precautions be taken to prevent exposure to these materials, for example, use of

controlled ventilation or isolation areas.

Always be aware of the possibility of contamination when using radioactive, toxic, or

pathogenic materials. Take all necessary precautions and use appropriate decontamination

procedures if exposure occurs.

Never use any material capable of producing flammable or explosive vapors or creating

extreme exothermic reactions.

Never exceed the maximum rated speed of the installed rotor; to do so can cause rotor

failure.

Always reduce (derate) rotor speed as instructed in this manual whenever:

•the rotor speed/temperature combination exceeds the solubility of the gradient

material and causes it to precipitate.

•the compartment load exceeds the maximum allowable compartment load specified.

See Chapter 2, Compartment Loads in Excess of Design Mass.

Failuer to reduce rotor speed under these conditions can cause rotor failure

Preface

Thermo Scientific T-647.5 iv

CAUTION

Do not expose aluminum rotor components to: strong acids, bases, or alkaline laboratory

detergents; liquid chlorine bleach; or salts (chlorides) of heavy metals such as cesium, lead,

silver, or mercury. Use of these materials with aluminum can cause a chemical reaction that

initiates corrosion.

Do not operate or precool a rotor at the critical speed, as this will have a detrimental effect

on centrifuge component life. See Chapter 2, Operation.

Do not operate the rotor unless it is symmetrically balanced as described in this manual.

Operating the rotor out of balance can cause damage to the centrifuge drive assembly.

Do not operate the rotor without the cover in position and locked in place and the rotor

locked to the centrifuge drive. See Chapter 2, Operation.

Always maintain the rotor in the recommended manner. The rotor and all accessories

should be clean and inspected prior to each run; do not use rotors showing signs of

corrosion or cracking. See Chapter 3, Care and Maintenance.

Do not autoclave or expose any aluminum rotor body and cover or expose any aluminum

rotor parts to temperatures in excess of 100°C.

Thermo Scientific T-647.5 1-1

DESCRIPTION

This manual provides you with the information you will need to operate and maintain your Thermo

Scientific T-647.5 Fixed-Angle Ultracentrifuge Rotor. If you encounter any problem concerning either

operation or maintenance that is not covered in the manual, contact Thermo Fisher Scientific for

assistance. In the United States, telephone toll free 1-866-9THERMO. Outside the United States,

contact your distributor or agent for Thermo Fisher Scientific products. Thermo Fisher Scientific

product information is available on our internet web site at http:// www.thermo.com/centrifuge .

Contents

•“Rotor Description” on page 1-2

•“Rotor Specifications” on page 1-2

•“Accessories” on page 1-3

1 DESCRIPTION

Rotor Description

1-2 T-647.5 Thermo Scientific

Rotor Description

The T-647.5 is a six-place fixed-angle ultracentrifuge rotor that can be used at speeds up to 47,500

rpm.1 The rotor body is machined from a titanium forging for strength and corrosion resistance. The

aluminum lid and locking nut are given a blue anodized finish for surface protection. The rotor is

tightly sealed during operation by the rotor lid and two O-rings - one that fits inside the rotor lid and

another around the bottom of the aluminum locking nut. The six tube compartments are bored at a

22.5° angle to the axis of rotation. A disc with alternative black and reflective segments attached to

the bottom of the rotor provides overspeed protection.

Rotor Specifications

1Speed in revolutions per minute (rpm) is related to angular velocity, w, according to the following:

Where w = rad/s. All further references in this manual to speed will be designated as rpm.

ω(rpm)=2π

------

⎝⎠

⎛⎞ rpm()0.10472()=

Table 1-1.Rotor Specifications

Rotor Type Fixed Angle

Maximum Speed (rpm) 47,500*

*With tubes filled with a non-precipitating homogenous solution having an average density of 1.2 g/ml or less.

Relative Centrifugal Force (RCF) at Max. Speed

- at rmaximum 9.93 cm 250,259

- at raverage 6.66 cm 167.847

- at rminimum 3.39 cm 85.436

K Factor at Maximum Speed 114

Critical Speed 1000 rpm

Tube Size (Maximum) 39 mm x 107 mm

Number of Tube Compartments 6

Nominal Tube Capacity 90 ml

Total Rotor Capacity (Nominal) 540 ml

Maximum Tube Compartment Mass 157.6 grams

Tube Angle 22.5°

Rotor Diameter 19.5 cm (7.5 in)

Rotor Weight (empty) 11 kg (24.2 lbs)

1 DESCRIPTION

Accessories

Thermo Scientific T-647.5 1-3

Accessories

Accessories Supplied

The accessories supplied with the T-647.5 Rotor, Catalog No. 54304 (rotor and complete accessories),

are listed in Table 1-2. Those items indicated by an asterisk (*) are not supplied with Catalog No.

08322, which is a T-647.5 with basic accessories only.

To order replacement accessories, telephone 1-866-9THERMO in the United States. Outside the

United States, contact the local representative, distributor or agent for Thermo Fisher Scientific

products. Be sure to provide a description of the part, the catalog number, plus the rotor model and

serial number.

Optional Multipiece Sealing Assembly

If you prefer, you can use the T-647.5 Rotor with thinwall poly-allomer tubes and aluminum sealing

cap assemblies. The sealing assemblies consist of a tube plug, a tube plug cover, a sealing nut, and a

post-fill screw. When the assembly is tightened, the cover crimps the top of the tube to form a seal. In

order to use the rotor with this sealing assembly, you must purchase all items separately: the T-647.5

Rotor with basic accessories only, Catalog No. 08322; Tool Kit, Catalog No. 52988; the tubes and the

sealing assembly components. Table 1-3 lists the catalog number and description of the tubes and

Note In order to seal the ULTRACRIMP< Tubes, (supplied with Catalog No. 54304) you must

have an ULTRACRIMP® Sealing Tool, Catalog No. 03920.

Table 1-2. Accessories Supplied

Qty. Catalog No. Description

8 03990*

*Not supplied with Catalog No. 08322 (basic accessories only).

ULTRACRIMP® Tubes, 90 ml (pkg/25)**

**Each package of tubes is supplied with an equal number of tube caps and tube plugs.

6 03998*Rotor Cap

1 03940*Tube Racks, 3-place (1 pair)

1 12826*ULTRACRIMP® Tube Removal Tool

1 51942 Rotor Stand

1 65937 Vacuum Grease

1 61556 Lubricant

1 51350 Overspeed Decal, 47 500 rpm (extra)

1 64668 Rotor Cover O-ring, (extra)

1 64667 Locking Nut O-ring, (extra)

1 52392 Rotor Cover Tool

1 52384 Centrifuge/Rotor Log Book

1 08326 Instruction Manual

1 DESCRIPTION

Accessories

1-4 T-647.5 Thermo Scientific

sealing assembly components required, and Table 1-4 lists the parts supplied in the Tool Kit. If you

have the tools listed, you need not purchase the kit; or, if necessary, any of the tools listed can be

ordered separately by catalog number given in the table..

Rotor Cover Removal Tool

The rotor cover of the T-647.5 Rotor is designed to fit the rotor body tightly to ensure a positive seal;

however, because of this tight fit, it is sometimes difficult to remove. The catalog number for this tool

is 52652; complete instructions for use of the tool are supplied with it.

Table 1-3.Optional Multipiece Sealing Assembly Components

Catalog No. Description

03140 Tubes, 100 ml, Polyallomer (25/Pkg)

52571 Sealing Cap Assembly (each); 6 required

Table 1-4.Tool Kit (Catalog No. 52988) for use with Optional Sealing Assembly

Catalog No. Description

51834 Tube Cap Vise

52013 Tube Removal Tool (for Catalog No. 03140)

52881 Torque Wrench

66376 Socket, 7/8 inch

00693 Bottle Removal Tool

Thermo Scientific T-647.5 2-1

OPERATION

This chapter contains the information necessary to prepare the T-647.5 Ultraspeed Rotor for operation

and includes important safety information.

Contents

•“Prerun Safety Checks” on page 2-2

•“Compartment Loads in Excess of Design Mass” on page 2-2

•“Critical Speed” on page 2-3

•“Rotor Precool” on page 2-3

•“Relative Centrifugal Force (RCF) Determination” on page 2-3

•“Chemical Compatibility” on page 2-7

•“Rotor Balancing” on page 2-7

•“Rotor Loading and Sealing” on page 2-8

•“Ultracentrifuge / Rotor Log Book” on page 2-9

2 OPERATION

Prerun Safety Checks

2-2 T-647.5 Thermo Scientific

Prerun Safety Checks

To ensure safe performance of the rotor, before every run you should:

•read the Safety Information Page in the front of this manual.

•make sure each tube compartment is clean and that there is no sign of corrosion.

•be sure the rotor itself is clean and shows no sign of corrosion or cracking. Also, make sure there

are no scratches or burrs around the rim of the rotor.

•check the centrifuge chamber and drive spindle to be sure they are clean and free of scratches and

burrs.

•verify that proper overspeed decal is firmly attached to bottom of rotor; decal should have 19

black segments (see page 3-4 for Overspeed Decal Replacement).

•check the chemical compatibility of all materials used (see Appendix).

•inspect the rotor cover O-rings for cracks, tears, or abrasions; replace if necessary. Be sure the

O-rings are properly lubricated.

•make sure the rotor cover is on and properly tightened and that the rotor cover tool has been

removed from the locking nut.

•be sure the proper environment has been selected for operation; for example, controlled

ventilation or isolation, if required.

•check the top speed capability of the tube (or bottle) being used; observe the CAUTION.

Compartment Loads in Excess of Design Mass

The maximum operating speed (see Specifications, page 1-2) is based on the recommended design

mass that has been established for this rotor, representing the maximum mass that each tube

compartment can contain at top speed. To prevent rotor failure, the total contents of each

compartment (including specimen, tube, rotor cap, tube cap, and tube plug) should not exceed the

recommended figure unless the rotor speed is reduced proportionately. (If using optional sealing

assembly, include the weight of the complete assembly.)

WARNING The stresses that this rotor withstands during centrifugation gradually

weaken the rotor body, increasing the possibility of rotor failure. Thermo Fisher Scientific

warranties this rotor for the expected life of the product; and, for your safety, recommends

that this rotor be withdrawn from service after it has been used for 5000 runs. Failure to

do so can cause rotor failure with subsequent sample loss and damage to the rotor and/or

centrifuge. If the material being processed is hazardous, take all necessary precautions to

prevent exposure should rotor failure occur.

CAUTION When using a tube or bottle assembly other than those supplied by Thermo

Fisher Scientific, be sure to check the top speed capability; when in doubt, do a test run for

the desired application. If using a Thermo Fisher Scientific tube (or bottle) assembly other

than those supplied with the rotor, refer to the Product Guide for the maximum speed.

Exceeding the top speed capability of the tube (or bottle) can result in its breakage.

2 OPERATION

Critical Speed

Thermo Scientific T-647.5 2-3

Strict adherence to the maximum allowable compartment mass or reduced speed is required to prevent

rotor failure. Observe the WARNING.

The design mass for each compartment of the rotor is 157.6 g at 47 500 rpm for the T-647.5 Rotor.

This figure is based on the use of a ULTRACRIMP® polyallomer tubes, Catalog No. 03990, filled with

a liquid at 1.2 specific gravity, plus all sealing assembly components. If the total compartment mass is

greater than that figure, use the following formula to determine the reduced speed:

Critical Speed

The critical speed is that speed at which any rotor imbalance will produce a driving frequency equal to

the resonant frequency of the rotating system (that is, the rotor and the centrifuge drive). At this speed,

the rotor may produce large amplitude vibrations which can be felt in the centrifuge frame. Mass

imbalance will contribute to increased vibration intensity at critical speed. Avoid operating rotor at

critical speed (see page 1-1). Operation at the critical speed will have a detrimental effect on centrifuge

component life. Observe the CAUTION.

Rotor Precool

If samples are routinely processed around 4°C or below, the rotor should be stored in a refrigerator or a

cold room. If this is not possible, the rotor can be precooled in a Thermo Scientific Ultracentrifuge.

Refer to the ultracentrifuge instruction manual for precooling directions. Be careful not to precool the

rotor at its critical speed.

Relative Centrifugal Force (RCF) Determination

Relative centrifugal force (RCF) refers to the force during centrifugation that moves the particulate

outward from the center of rotation. This force is proportional to the radial distance and the square of

the rotor speed. The RCF value, also known as g force, is determined by the following formula:

when r = the radius in centimeters from the centerline of the rotor to the point in the tube where

RCF value is required

and rpm = the rotor speed in revolutions per minute

WARNING Always reduce (derate) rotor speed whenever the compartment load exceeds

the maximum allowable compartment load specified, or the average fluid density is greater

than 1.2 g/ml. Failure to reduce rotor speed under these conditions can cause rotor failure.

Reduced Speed Maximum Speed 157.6 g

actual weight (g)

--------------------------------------=

CAUTION Do not operate or precool the rotor at the critical speed, as this will have a

detrimental effect on centrifuge component life.

RCF 11.17(r)=rpm

1000

------------

⎝⎠

⎛⎞

2 OPERATION

Relative Centrifugal Force (RCF) Determination

2-4 T-647.5 Thermo Scientific

Figure 2-1 shows the minimum, average, and maximum radii of the T-647.5 Rotor. Tables 2-1 gives

the RCF value at each radius at speeds from 20 000 rpm to maximum speed (in increments of 500

rpm). The RCF value at any other speed can be calculated by using the above formula.

Figure 2-1. Rotor Radii

The time required to sediment a particle in water at 20°C through the maximum rotor path length

(that is, the distance between rminimum and rmaximum) can be calculated using the equation:

where:

t = sedimentation time in hours

K = the clearing factor for the rotor (defined on the next page)

S20, w = the sedimentation coefficient for the particle of interest in water at 20°C as expressed

in Svedbergs1

Note The radii values given are the actual rotor specifications. These values do not take the

thickness of the tube into consideration.

1 The sedimentation coefficient (S) in seconds, for a particle in a centrifugal field is defined by the equation S =

(dx/dt) [1/(ω2x)]; where dx/dt = sedimentation velocity of the particle in cm/s; ω = rotor speed in rad/s; and x

= the distance of the particle from the axis of rotation in centimeters. Conventionally, experimentally

determined values of sedimentation coefficients are multiplied by 1013 to convert them to Svedberg units (S),

so a particle with an experimentally determined sedimentation coefficient of 10-11 seconds is usually referred to

in the literature as a "100 S particle." Since the value determined for the sedimentation coefficient is dependent

on the density and viscosity of the solution in which centrifugation is performed, values are usually reported for

the standard conditions of infinite dilution in water at 20°C, and designated S20, w.

S20,w

-------------

2 OPERATION

Relative Centrifugal Force (RCF) Determination

Thermo Scientific T-647.5 2-5

The clearing (or K) factor is defined by the equation:

Where rmaximum and rminimum are the maximum and minimum rotor radii, respectively, and rotor speed

is expressed in rpm.

K factors for the T-647.5 Rotor, at speeds from 20 000 rpm to maximum speed (in increments of 500

rpm), are listed in Table 2-1.

Example: The T-647.5 Rotor has a K factor of 120.7 at the maximum permitted speed (47 500 rpm).

If the particles to be sedimented have a sedimentation coefficient of 10S, the estimated run

time required at maximum speed will be:

Note that the calculation assumes particles in water at 20°C. If the suspending medium is denser or more viscous than

water, the sedimentation time will be greater.

Table 2-5. T-647.5 Rotor: RCF Values and K Factors*

Speed (rpm) rmax. 9.93 cm

RCF

ravg. 6.66 cm rmin. 3.39 cm K Factors

20,000 44,367 29,757 15,147 680

20,500 46,613 31,263 15,913 647

21,000 48,915 32,807 16,699 617

21,500 51,272 34,388 17,504 588

22,000 53,684 36,006 18,327 562

22,500 56,152 37,661 19,170 537

23,000 58,676 39,353 20,031 514

23,500 61,255 41,083 20,912 492

24,000 63,889 42,850 21,811 472

24,500 66,579 44,654 22,729 453

25,000 69,324 46,495 23,666 435

25,500 72,124 48,374 24,623 418

26,000 74,981 50,289 25,598 402

26,500 77,892 52,242 26,592 387

27,000 80,859 54,232 27,605 373

27,500 83,882 56,259 28,636 360

28,000 86,960 58,323 29,687 347

28,500 90,093 60,425 30,757 335

29,000 93,282 62,564 31,846 323

29,500 96,526 64,740 32,953 312

30,000 99,826 66,953 34,080 302

K 253000()In rmaximum

rminimum

--------------------

⎝⎠

⎛⎞

rotor speed

1000

-------------------------

⎝⎠

⎛⎞

÷=

t121

10S

---------

=12.10 hours 6 hours=

2 OPERATION

Relative Centrifugal Force (RCF) Determination

2-6 T-647.5 Thermo Scientific

30,500 103,182 69,203 35,225 292

31,000 106,592 71,491 36,390 283

31,500 110,058 73,816 37,573 274

32,000 113,580 76,178 38,775 266

32,500 117,157 78,577 39,996 257

33,000 120,790 81,013 41,236 250

33,500 124,477 83,486 42,495 242

34,000 128,221 85,997 43,773 235

34,500 132,020 88,545 45,070 228

35,000 135,875 91,130 46,386 222

35,500 139,785 93,753 47,721 216

36,000 143,750 96,412 49,075 210

36,500 147,771 99,109 50,447 204

37,000 151,847 101,843 51,839 199

37,500 155,979 104,614 53,249 193

38,000 160,166 107,422 54,679 188

38,500 164,408 110,268 56,127 183

39,000 168,706 113,151 57,595 179

39,500 173,060 116,070 59,081 174

40,000 177,469 119,028 60,586 170

40,500 181,933 122,022 62,110 166

41,000 186,453 125,053 63,653 162

41,500 191,029 128,122 65,215 158

42,000 195,660 131,228 66,796 154

42,500 200,346 134,371 68,396 151

43,000 205,088 137,551 70,015 147

43,500 209,885 140,769 71,653 144

44,000 214,737 144,023 73,309 140

44,500 219,646 147,315 74,985 137

45,000 224,609 150,644 76,679 134

45,500 229,628 154,010 78,393 131

46,000 234,703 157,414 80,125 129

46,500 239,833 160,855 81,876 126

47,000 245,018 164,332 83,647 123

47,500 250,259 167,847 85,436 121

Table 2-5. T-647.5 Rotor: RCF Values and K Factors*

Speed (rpm) rmax. 9.93 cm

RCF

ravg. 6.66 cm rmin. 3.39 cm K Factors

2 OPERATION

Chemical Compatibility

Thermo Scientific T-647.5 2-7

Chemical Compatibility

The critical components of the T-647.5 Rotor likely to come in contact with solution are: rotor body

(titanium), rotor cover assembly (aluminum), rotor cap and tube cap (aluminum), tube plug (Buna N),

rotor cover O-ring and locking nut O-ring (Viton®), plus the material of the tubes (or bottles) being

used. The components of the optional sealing assembly are: tube plug cover and tube plug (titanium);

post-fill screw (stainless steel); plug (nylon); O-ring (Viton®); and tube (polyallomer).

The chemical compatibility of rotor elements and accessory materials is given in the Appendix. Because

no organized chemical resistance data exists for materials under the stress of centrifugation, this data is

intended to be used only as a guide. When in doubt, we recommend pretesting of sample lots.

Rotor Balancing

Always balance the rotor according to the following criteria:

a. balance pairs of tubes containing fluid of identical specific gravity to within 0.5 gram and place

them in opposing tube compartments;

b. when using less than a full complement of six tubes, the rotor can be operated at its maximum

allowable speed with two, or four samples, if opposing pairs of tubes are positioned as shown

in figure 2-2;

c. if one, three, five, or seven samples are to be run, balance the load as above with a tube that

contains a solution of the same specific gravity as the sample in the opposing tube

compartment.

Figure 2-2. Compartment Loading

*RCF and K Factor are based on radii values that do not take the thickness of the tube into consideration.

CAUTION Do not operate the rotor unless it is symmetrically balanced as described in this

manual. Operating the rotor out of balance can cause damage to the centrifuge drive

assembly.

Note The configuration for THREE tubes has the tubes unopposed. In this case, all three tubes

must be equally balanced.

2 OPERATION

Rotor Loading and Sealing

2-8 T-647.5 Thermo Scientific

Rotor Loading and Sealing

Prepare the ULTRACRIMP® tubes for use by following the tube filling and tube sealing procedures

given in the ULTRACRIMP® Tube Sealing System Instruction Manual.

Load and seal the rotor as follows:

1. Gently place the filled ULTRACRIMP® tubes in the tube compartments, balancing the rotor as

described above.

2. Place a rotor cap (Catalog No. 03998) in each tube compartment that contains a filled tube.

Push the rotor cap into the tube compartment until it is properly seated; when properly seated,

the edge of the cap will approximately flush with, or slightly below, the top of the outer edge of

the tube compartment. See figure 2-3. Read the CAUTION.

Figure 2-3. Properly Seated Rotor Cap

Note If using the optional tubes and sealing assemblies (Catalog No. 03140 and 52571

respectively), seal the tubes and load them in the rotor according to the instructions supplied with

the sealing cap assemblies.

Note Do not put a rotor cap in an empty tube compartment. Rotor caps should only be

installed in compartments that contain a filled tube.

Rotor caps must be used and properly seated in the rotor to provide tube support during

centrifugation.

2 OPERATION

Ultracentrifuge / Rotor Log Book

Thermo Scientific T-647.5 2-9

3. Separate the locking nut from the rotor cover, and check locking nut O-ring and rotor cover

O-ring for scuffs, cracks or breaks. Replace, if necessary (see Figure 3-1 for replacement part

catalog numbers).

4. Coat O-rings lightly with vacuum grease, Catalog No. 65937.

5. Check that the locking nut thread is lightly lubricated with grease, Catalog No. 61556.

6. Place the rotor cover (with O-ring) on the rotor body. Insert the locking nut in the cover and finger

tighten the locking nut by turning it clockwise. Insert the rotor cover tool through the locking nut.

Hold the cover to prevent it from turning, and continue turning the locking nut clockwise until

rotor cover is tightened securely.

7. Remove the rotor cover tool from the locking nut. Read the CAUTION.

8. Carry the rotor carefully, and lower it into the centrifuge chamber slowly and vertically. Be sure it

snaps in place on the drive adapter. Check that the rotor is properly seated by pulling it gently

upward and noting a small amount of resistance.

Perform the run as explained in the centrifuge instruction manual.

Ultracentrifuge / Rotor Log Book

An Ultracentrifuge/Rotor Log Book is supplied with the T-647.5 so that you can easily record all data

necessary to meet the warranty stipulation that any defective ultracentrifuge rotor (or ultracentrifuge)

returned to Thermo Fisher Scientific must be accompanied by a current history of the rotor (see

Warranty Statement in the Appendix).

Each time the T-647.5 Rotor is used, record the run in the appropriate log book. If desired, the

information may be recorded elsewhere, however, it must include all data as shown in figure 2-4,

Sample Centrifuge/Rotor Log Sheet.

Figure 2-4. Sample Centrifuge/Rotor Log Sheet

CAUTION The rotor cover tool must be removed prior to centrifuge run.

Thermo Scientific T-647.5 3-1

CARE and MAINTENANCE

This chapter provides instructions on how to clean, decontaminate, and maintain your rotor. Always

maintain the rotor in the recommended manner. Do not use rotors that show signs of corrosion or

cracking.

Contents

•“Corrosion” on page 3-2

•“Cleaning” on page 3-2

•“Storage” on page 3-4

•“Overspeed Decal Replacement” on page 3-4

•“Service Decontamination Policy” on page 3-5

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Thermo Fisher Scientific T-647.5 Fixed Angle Rotor User manual

Brand: Thermo Fisher Scientific

Model: T-647.5 Fixed Angle Rotor

Category: Fridge-freezers

Type: User manual

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Thermo Fisher Scientific T-647.5 Fixed Angle Rotor User manual

Thermo Fisher Scientific T-647.5 Fixed Angle Rotor User manual

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