The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL,
INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR
USE OF IT.
Revision history: MAN0010919
Revision Date Description
C.0 8 January 2017 • Ion PGM™ Calibration Standard removed from kit components list. It is now ordered
separately (Cat. No. A27832).
• Recommendation for dilution of
E. coli
DH10B Control 400 Library added.
• “Load the Ion Chef™ System“ and “Clean the Ion Chef™ Instrument“ topics reorganized
for ease of use.
• “Create a Planned Run“ on page 20 updated for Torrent Suite™ Software v5.2
• Designation of Chip-loading centrifuge positions changed from "A" and "B" to "1" and
"2".
• ISP quality control sections updated and moved to new user guides:
–
Ion Sphere
™
Assay on the Qubit
™
2.0 Fluorometer User Guide
(Pub. No.
MAN0016387)
–
Ion Sphere
™
Assay on the Qubit
™
3.0 Fluorometer User Guide
(Pub. No.
MAN0016388)
• Web links updated.
B.0 28 January 2016 • Guidance added for use with Torrent Suite™ Assay Development Software
• Library input recommendations added for Ion AmpliSeq™ for Chef DL8 Kit users.
• Updated for Torrent Suite™ Software v5.0
• Instructions added to override warning for no Torrent Server or network connection
•Clarifications made in the sequencing workflow, including use of the Line Clear feature
in initialization.
A.0 6 February 2015 New user guide, which includes instructions to set up and operate the Ion Chef™ System with
the new Ion PGM™ Hi‑Q™ Chef Kit (Cat. No. A25948).
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the
terms and conditions of all applicable Limited Use Label Licenses.
Corporate entity: Life Technologies Corporation | Carlsbad, CA 92008 USA | Toll Free in USA 1 800 955 6288
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Microsoft, Windows, and
Excel are trademarks of Microsoft Corporation. Macintosh is a trademark of Apple, Inc. Guava and easyCyte are trademarks of Guava Technologies,
Inc. Cy is a trademark of GE Healthcare UK Limited. Agencourt and Ampure are trademarks of Beckman Coulter, Inc. Bioanalyzer is a trademark of
Agilient Technologies, Inc. ELGA and PURELAB are trademarks of VWS Limited. Luer-Lok is a trademark of of Becton, Dickinson and Company
Corporation. Orion is a trademark of Thermo Orion, Inc. Kimwipes is a trademark of Kimberly-Clark Corporation.
©2017 Thermo Fisher Scientific Inc. All rights reserved.