Thermo Fisher Scientific Large-Capacity Reach-In CO2 Incubator User manual

Brand: Thermo Fisher Scientific

Model: Large-Capacity Reach-In CO2 Incubator

Type: User manual

Visit us online to register your warranty

www.thermoscientific.com/labwarranty

User Manual

Forma 29 cu ft Reach-In Incubator

Model 3950 and 3951

Operating and Maintenance Manual 7003950 Rev. 27

Thermo Scientific

Preface

Reach-In Incubator i

Manual Number 7003950

27 41847 11/01/17 Added Intended and NonIntended use statement bpg

26 40087/IN-4743 7/27/16 Changed the label in the remote alarm artwork to a note - pg 6-1 ccs

25 40639 5/3/16 Updated for UL (G Smith), added risk assessment info ccs

24 31580/IN-4675 10/5/15 Motor chg - part number, expl dwg, schematic ccs

23 31289 7/18/14 Removed CE reference on pg 7-3 ccs

22 30374/IN-4419 4/22/14 Updated 1900008-06 drawing - pg 8-11 (left hand door swing) ccs

21 29318//IN-4272 12/14/12 Clarified usage on exploded drawing 3950-08-4 list ccs

20 28773/IN-4231 10/8/12 Updated Figure 1-6 to current ccs

* T/C is a thermal conductivity sensor.

** All units are 50/60 Hz.

Covered

Models CO2Sensor* Voltage**

3950 T/C 100-120

3951 T/C 200-230

Thermo Scientific

ii Reach-In Incubator

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Warning If the incubator is not used in the manner specified in this operating manual, the protection provided

by the equipment design may be impaired. s

Caution When operating this unit under high temperatures and/or relative humidity (RH), caution must be

observed when the unit is shut down, unexpectedly or by choice. Condensate can form on the interior of the

chamber as it cools. This includes the heating element surfaces. Heavy condensate can bridge the ceramic

insulators and cause a temporary electrical short to ground. This electrical circuit is protected by a circuit

breaker. However, to avoid this contingency if these conditions occur, open the door of the unit and allow it to

stand open until the water vapor disperses. s

Intended Use: The Thermo Scientific Large Capacity Reach in CO2 Incubators are intended to maintain an

optimal environment for the incubation of cells and tissues for scientific research and clinical applications.

These models maintain temperature and carbon dioxiode (CO2) levels as set by the operator with increased

relative humidity.

Non-Intended Use: The 310 Series Direct Heat Incubators are not intended for use where electrical or physical

contact with the patient is established. Not intended for use by the general public. Not intended for use within

the patient environment. Not to be used outdoors. Not intended to be operated in potentially explosive

environments or for use with flammable materials.

Material in this manual is for information purposes only. The contents and the product it describes are subject

to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Thermo Scientific Reach-In Incubator iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Lifting Hazard Warning. The incubator weighs more than 200lbs (91kgs). Take adequate safety

measures when moving this device.

WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in

each EU Member State. For further information, send an email to [email protected].

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Reach-In Incubator

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

1-800-438-4851 Toll Free, U.S. and Canada

1-877-213-8051 FAX

http://www.thermofisher.com Internet Worldwide Web Home Page

service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road, Box 649

Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Reach-In Incubator v

Preface

Warranty Notes

Information You Should Know Before Requesting Warranty Service

•Locate the model and serial numbers. A serial tag is located on the unit itself.

• For equipment service or maintenance, or with technical or special application inquiries, contact Technical

Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local

distributor.

Repairs NOT Covered Under Warranty

• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for

temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as

a billable service at your location. Calibration after a warranty repair is covered under the warranty.

• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to

equipment materials.

• Service calls for improper installation or operating instructions. Corrections to any of the following

are billable services:

1) electrical service connection

2) tubing connections

3) gas regulators

4) gas tanks

5) unit leveling

6) room ventilation

7) adverse ambient temperature fluctuations

8) any repair external to the unit

• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper

installation.

• Repairs to parts or systems resulting from unauthorized unit modifications.

• Any labor costs other than that specified during the parts and labor warranty period, which may

include additional warranty on CO2sensors, blower motors, water jackets, etc.

Reach-In Incubator viThermo Scientific

Table of Contents

Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Control Panel Keys, Displays, & Indicators . . . . . . . . . . . . . . . . . . . . 1-2

Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Unit Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Preliminary Cleaning and Disinfecting . . . . . . . . . . . . . . . . . . . . . . 1-4

Installing the Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5

Attaching the Drain Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

The Water Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Filling the Humidity Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Connecting the Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . 1-7

Connecting the CO2Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9

Setting the Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . . 1-9

Setting the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10

Setting the CO2Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Calibrating the CO2System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Disable the ADD WATER Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Turn All Audible Alarms ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1

Set an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Set a Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Enable Temp Alarms to Trip Relay Contacts . . . . . . . . . . . . . . . . . . .3-3

Set Low CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Set High CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Enable CO2Alarms to Trip Relay Contacts . . . . . . . . . . . . . . . . . . . .3-4

Set New Zero No. for New CO2Sensors . . . . . . . . . . . . . . . . . . . . . .3-5

Set New Span No. for CO2Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5

Enable the High Humidity Feature . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

Select a Primary Tank w/ Gas Guard Option . . . . . . . . . . . . . . . . . . 3-6

Disable the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7

Set RS485 Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7

Section 1

Section 2

Section 3

vii Reach-In Incubator Thermo Scientific

Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning the Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning the Humidity Reservoir . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Replacing the Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Discarding/Removing Incubator from Service . . . . . . . . . . . . . . . . . . 5-3

Factory Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1

Connect the Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Connect the RS485 Interface (P/N 190523) . . . . . . . . . . . . . . . . . . . 6-2

Connect Analog Output Boards (P/N 190512, 190543, & 190544) .6-3

CO2Gas Guard (P/N 1900000) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4

Connect CO2Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

De-activate Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5

Operation of CO2Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Inner Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-6

Shaker Support Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-6

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1

Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

Assembly Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2

Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-3

Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9-1

Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-1

Table of Contents

Section 4

Section 5

Section 6

Section 8

Section 7

Section 9

Section 10

Reach-In Incubator 1-1Thermo Scientific

Section 1 Installation and Start-Up

Figure 1-1. Front View Figure 1-2. Side View

Figure 1-3. Back View

Figure 1-4. Top View

1-2 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

Figure 1-4. Control Panel Components

Tank Low Indicator - Used with Gas Guard, lights when gas tank is low,

if applicable.

Silence - Mutes the audible alarm. See Section 4 for ringback times.

Alarm Indicator - Light pulses on/off during an alarm condition in the

cabinet.

Mode Select Switch - Used to select Run, Setpoints, Calibration and

System Configuration Modes.

Message Center - Displays system status.

Mode Select Indicators -

• Run: Run Menu

• Set: Set Points Menu

• Cal: Calibrate Menu

• Config: Configuration Menu

Up and Down Arrows - Increases or decreases the number values, toggles

between parameter values of the selected mode.

Enter - Stores the changed value into computer memory.

Heat Indicator - Lights when power is applied to the heaters.

Temp Display – Displays temperature continuously

Scroll for Parameters Arrows - Moves the operator through the parame-

ters of the selected mode. Right arrow goes to next, left arrow goes to

previous parameter.

CO2Inject Indicator - Lights when CO2is being injected into the incu-

bator.

% CO2 Display - Displays CO2percentage continuously.

Control Panel Keys,

Displays, & Indicators

Tank Low

Reach-In Incubator 1-3Thermo Scientific

Section 1

Installation and Start-Up

The Model 3950 Series Reach-In Incubator has four basic modes that

allow incubator setup: Run, Setpoints, Calibration and System

Configuration.

• Run is the default mode during normal operation.

• Set is used to enter system setpoints.

• Calibration is used to calibrate various system parameters.

• Configuration allows for custom setup of various options.

The chart below shows the selections under each of the modes.

Keypad Operation

RUN SETPOINT CALIBRATION CONFIGURATION

Default Mode Temperature Temp Offset Audible

Overtemp CO2 Cal Access Code

CO2 Temp Lo Alarm

Temp Relay

CO2 Lo Alarm

CO2 Hi Alarm

CO2 Relay

CO2 Z & S #'s

RH High On/Off

H20 Alarm On/Off

Tank Select

Gas Guard

RS485 Address

**Base Unit Displays

**Option Displays

Caution Units must be installed against a wall or similar structure in an

area of minimum ambient temperature fluctuation. A minimum of six (6)

inches clearance is required at the top and back of the incubator plus a

minimum three (3) inch clearance on each side for electrical, water and gas

hook-ups. At least eight (8) inches clearance above the cabinet is required

for service access. s

Locate the unit on a firm level surface capable of supporting the unit

weight of approximately 500 lbs.

Position the incubator away from doors and windows and heating and air

conditioning ducts.

Warning This incubator weighs approximately 500 lbs. Have sufficient

personnel available when moving. s

Disinfect all interior surfaces with a general-use laboratory disinfectant,

such as quaternary ammonium, to remove any residue from the production

of the incubator. Rinse thoroughly with sterile distilled water, then 70%

alcohol. Dry with a sterile cloth as needed. Disinfect the shelf channels and

shelves, then rinse with distilled water before installing.

Caution Before using any cleaning or decontamination method except

those recommended by the manufacturer, users should check with the

manufacturer that the proposed method will not damage the equipment.

Accidental spills of hazardous materials on or inside this unit are the

responsibility of the user. s

The shelves may be installed at any level in the incubator. Install a shelf

channel on each side. With the tabs

pointing up, attach the channel by

locating the rivet into a slotted hole, far

end first. Pull the channel toward the

front and slide the front rivet on the

channel into the slotted hole and press

down. Make sure that the channels are

opposite each other so that the installed

shelf will be level.

Place a bubble-type level on a shelf inside the incubator. Adjust the feet as

needed; counterclockwise to lengthen or clockwise to shorten. Level the

unit front-to-back and left-to-right.

1-4 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

Preliminary Cleaning and

Disinfecting

Unit Installation

Leveling the Unit

Installing the Shelves

Figure 1-5. Shelf Channel

Attaching the Drain Lines Note Drain connections on the back of the unit are shipped capped. If

humidity is not required, the caps should be left on the drains. s

If manual or gravity feed method is used for humidity in the cabinet,

connecting the drains is optional.

If an in-house pure water supply is used to fill the reservoir, it is

recommended that the drains be connected to a floor drain.

For best operation of the incubator, sterilized distilled, demineralized or

de-ionized water should be used in the humidity reservoir. Water purity

should be in the resistance range of 50K to 1M Ohm/cm, or a

conductivity range of 20.0 to 1.0 uS/cm. Refer to ASTM Standard

D5391-93 or D4195-88 for measuring water purity.

Distillation systems, as well as some types of reverse osmosis water purity

systems, can produce water in the quality range specified. Tap water is not

recommended as it may contain chlorine, which can deteriorate the

stainless steel. Tap water may also have a high mineral content, which

would produce a build-up of scale in the reservoir. High purity or ultra

pure water is not recommended as it is an extremely aggressive solvent and

will deteriorate the stainless steel. High purity water has a resistance of

above 1M to 18M Ohm. Even high purity water can contain bacteria and

organic contaminants. Water should always be sterilized or treated with a

decontaminant, safe for use with stainless steel as well as safe for the

product, prior to being introduced into the humidity reservoir.

Reach-In Incubator 1-5Thermo Scientific

Section 1

Installation and Start-Up

Back of Unit

1/4” Water Inlet fitting

to 1/4” ID tubing

to in-house water supply

shut-off valve (30 PSIG max.)

Chamber Drain (Overflow) -

3/8” elbow with 3/8” hose barb fitting.

Install with fitting sloping slightly downward.

Condensate Drain -

3/8” fitting

to 3/8” ID hose

Back View

Floor

Figure 1-6. Top View of Fitting Connections

The Water Supply

In-house Pure Water

Supply

Filling the Humidity

Reservoir

Caution Distilled or de-ionized water used in the humidity reservoir must

be within a water quality resistance range of 50K to 1M Ohm/cm to

protect and prolong the life of the stainless steel. Use of water outside the

specified range will decrease the operating life of the unit and may void

warranty. s

The incubator is equipped with a liquid level float switch that detects low

water levels in the humidity reservoir and produces an alarm condition. As

all applications do not require humidity, this alarm can be disabled. To

disable the ADD WATER alarm, see Section 3. If humidity is required in

the current application, press the Silence key on the control panel to mute

the alarm while the humidity reservoir is being filled.

There are three methods for filling the humidity reservoir; an in-house

pure water supply, the manual and the gravity feed methods.

All incubators provide a connection for using an in-house water supply.

Connect a low pressure (not more than 30 PSI) water line, equipped with

a manual shut-off valve, to the inlet fitting on the float valve reservoir. The

1/4” hose connection is located on the rear of the incubator cabinet. With

the water turned on, a float valve inside the incubator regulates the water

level in the humidity reservoir. When the reservoir is full, a 10-second

audible tone is heard and the ADD WATER message no longer displays.

This fill system consists of a funnel attached to the side duct sheet and

tubing located inside the incubator. Disinfect the funnel and rinse with

sterile water before using. This funnel may be moved to a preferred

location by unscrewing the well nuts and disconnecting it from the

sidewall. Tighten the nuts after relocation. The holes allotted for funnel

use are slightly larger and in a centralized area. See Figure 1-6.

To fill the reservoir, pour sterile distilled water (approximately 2.5 gallons)

into the funnel. When the float switch trips, a 10-second audible tone

sounds and the ADD WATER message no longer displays. Add 1 gallon

after the 10-second tone is heard.

1-6 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

The Water Supply

(continued)

Manual Method

An optional carboy water fill system is also available. The carboy option

kit includes an instruction sheet that details the installation of the system.

See the serial tag on the side of the unit or the Specifications section for

electrical specifications. Refer to the electrical schematics at the end of this

manual.

Warning Connect the incubator to a grounded, dedicated circuit. The

power cord connector is the mains disconnect device for the incubator.

Position the incubator so the unit can be easily disconnected. s

Plug the provided power cord into the power inlet connector on the back

of the cabinet, then into a grounded, dedicated electrical circuit.

The Model 3950 Series also has an internal outlet located on the right side

of the interior back wall. The outlet is to provide power (230W

maximum) to accessory equipment. This outlet is not to be used when the

temperature is above 40°C and the RH more than 50%.

Reach-In Incubator 1-7Thermo Scientific

Section 1

Installation and Start-Up

Gravity Feed Method

Pour in sterile,

distilled water

Center Front

Unit Sidewall

Figure 1-6. Manual Feed Method with Funnel

Connecting the Unit

to Electrical Power

Manual Method

(continued)

Warning High concentrations of CO2gas can cause asphyxiation.

OSHA Standards specify that employee exposure to carbon dioxide in any

8-hour shift of a 40-hour work week shall not exceed the 8-hour time

weighted average of 5000 PPM (0.5% CO2). The short-term exposure

limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide

monitors are recommended for confined areas where concentrations of

carbon dioxide gas can accumulate.

This incubator is designed for CO2gas only. Connecting a flammable or

toxic gas could result in a hazardous condition.

Gases other than CO2should not be connected to this equipment. CO2

gas cylinders have a UN1013 label on the cylinder and are equipped with a

CGA 320 outlet valve. Check the gas cylinder for the proper identification

labels. The CO2gas supply being connected to the incubator should be

industrial grade, 99.5% pure. Do not use CO2gas cylinders equipped with

siphon tubes. A siphon tube is used to extract liquid CO2from the

cylinder, which can damage the pressure regulator. Consult with your gas

supplier to ensure that the CO2cylinder does not contain a siphon tube.

Gas cylinders should also be secured to a wall or other stationary object to

prevent them from tipping.

A two-stage CO2pressure regulator is required to be installed on the outlet

valve of the gas cylinder. Input pressure to the incubator must be

maintained at 15 psig (103.4 kPa) for proper performance of the CO2

control system. A single stage CO2pressure regulator will not maintain 15

psig (103.4 kPa) to the incubator as the pressure in the CO2cylinder

decreases; therefore, a two-stage regulator is recommended.

If higher purity CO2is desired inside the incubator (greater than 99.5%

pure), the pressure regulator should be constructed with a stainless steel

diaphragm, along with specifying the purity of the CO2from the gas

supplier. Follow the manufacturer’s instructions to ensure proper and safe

installation of the pressure regulator on the gas cylinder.

Consult your facility safety officer to ensure that the equipment is installed

in accordance with the codes and regulations applicable in your area. s

The CO2gas supply being connected should be industrial grade 99.5%

pure and should not contain siphon tubes. Install a two-stage pressure

regulator at the cylinder outlet. The high pressure gauge at the tank should

have 0-2000 psig range. The low pressure gauge, at the incubator inlet,

should have a 0-30 psig range. Input pressure to the incubator must be

maintained at 15 psig (103.4 kPa), ±5 psig.

Caution Each tank should be connected to one incubator only. s

1-8 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

Connecting the CO2

Gas Supply

Setting the Operating

Temperature

Incubator Start-Up

The incubator has serrated fittings on the back of the cabinet to connect

the gas supply (Figure 1-2). The fitting is labeled CO2 Inlet #1 Tank.

Secure connections with clamps. Check all fittings for leaks.

For units having the CO2Gas Guard option, refer to Section 6.

With the incubator properly installed, connected to power, the humidity

reservoir filled and the unit connected to a gas supply, system setpoints can

be entered. The following setpoints can be entered in Set Mode:

Temperature, Overtemperature and CO2. To enter Set Mode, press the

Mode key until the Set indicator lights. Press the right and/or left arrow

keys until the proper parameter appears in the message display center. See

Chart 1-1 for more detail.

This incubator has an operating temperature setpoint range of 5.0°C above

ambient to 60.0°C. It is shipped from the factory with a temperature

setpoint of 10.0° C. At this setting, all heaters are turned off. To change

the operating temperature setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until “Temp XX.X” is displayed in the message

center.

3. Press up/down until the desired temperature setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights for Run mode or

press the right/left arrow keys to go to next/previous parameter.

Caution Any equipment placed inside chamber must be rated for unit

operating temperature and humidity. s

Reach-In Incubator 1-9Thermo Scientific

Section 1

Installation and Start-Up

Connecting CO2Gas

Supply (continued)

Warning The independent overtemp system is designed as a safety to

protect the incubator only. It is not intended to protect or limit the

maximum temperature of the cell cultures or customer’s equipment inside

the incubator if an overtemp condition occurs. s

All Model 3950 Series incubators are equipped with a secondary

temperature monitoring system to monitor the air temperature inside the

cabinet. This system is designed as a safety device to turn off all heaters in

the event of a temperature control failure. The temperature control point

in the incubator will be approximately ±1°C of the overtemp setpoint.

The overtemp setpoint is set by the factory (default) at 40°C. However, the

overtemp can be reset over a range from 0.5°C above the operating

temperature setpoint to 65°C.

If the incubator’s operating temperature setpoint is set above the overtemp

setpoint, the overtemp setpoint will automatically update to 1°C above the

temperature setpoint. It is recommended that the overtemp setpoint be

maintained at 1°C over the operating temperature setpoint.

To set the Overtemp setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until Otemp XX.X is displayed in the message

center.

3. Press the up or down arrow until the desired Otemp setpoint is

displayed.

4. Press Enter to save the setting.

5. Press the Mode key until the Run indicator lights, or press the right or

left arrow to go to the next or previous parameter.

All CO2cells are calibrated at the factory at 37°C, medium humidity and

10% CO2. Therefore, if a temperature setpoint of 37°C has been entered,

the humidity reservoir has been filled and the CO2control is set to run

between 0-10%, the CO2setpoint may be entered immediately. Otherwise,

it is important to allow the unit 12 hours to stabilize at the temperature

setpoint before entering the CO2setpoint.

1-10 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

Setting the Overtemp

Setpoint

Setting the CO2Setpoint

All models of the incubator have a CO2setpoint range of 0.0% to 20.0%.

The incubator is shipped from the factory with a CO2setpoint of 0.0%.

At this setting, all CO2control and alarms are turned off.

To change the CO2setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until “CO2 XX.X” is displayed in the message

center.

3. Press the up/down arrow until the desired CO2setpoint is displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights to go to Run mode,

or press the right/left arrow keys to go to next/previous parameter.

Reach-In Incubator 1-11Thermo Scientific

Section 1

Installation and Start-Up

Setting the CO2Setpoint

(continued)

1-12 Reach-In Incubator Thermo Scientific

Section 1

Installation and Start-Up

Press MODE to light SET

Silence Mode

Mode

SET POINTS

TEMP XX.X C

CO2 XX.X%

36.9

4.9

Enter

Numbers increase

Press MODE to move

to CALIBRATE

mode

Numbers decrease

Press Enter

to save setting

Press Enter

to save setting

Press Enter

to save setting

Run Set Cal Config Heat Temp

Inject % CO2

Scroll for Parameters

Mode OTEMP XX.X C EEnntteerr

Scroll for Parameters

Press to return

to previous parameter

Press to return

to previous parameter

Press to return

to previous parameter

Percent CO2

Over

Temperature

Operating

Temperature

To Se t:

Set Mode

Chart 1-1

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Thermo Fisher Scientific Large-Capacity Reach-In CO2 Incubator User manual

Brand: Thermo Fisher Scientific

Model: Large-Capacity Reach-In CO2 Incubator

Type: User manual

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Thermo Fisher Scientific Large-Capacity Reach-In CO2 Incubator User manual

Thermo Fisher Scientific Large-Capacity Reach-In CO2 Incubator User manual

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