Thermo Scientific 360 User manual

Brand: Thermo Scientific

Model: 360

Type: User manual

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User Manual

Forma Direct Heat CO2 Incubator

Model 310 Series

Operatign and Maintenance Manual 7010310 Rev. 16

Thermo ScientificThermo Scientific

Preface

Model 310 Series DH Incubator i

Preface

Model CO2 Sensor* Voltage**

310 T/C 115

311 T/C 230

320 IR 115

321 IR 230

350† T/C 115

351† T/C 230

360† IR 115

361† IR 230

* T/C is a thermal conductivity sensor.

IR is an infrared sensor.

** All units are 50/60 Hz.

† Copper interior

MANUAL NUMBER 7010310

16 31343/IN-4592 10/22/14 New magnetic door switch (exploded drawing, electrical schematics) RoHS ccs

15 29174/IN-4469 3/21/14 Solenoid change - update 203 drawing and schematics (pgs 8-5 & 9-1 to 9-3) ccs

14 30649/IN-4446 1/28/14 Updated electrical schematics - display board ccs

13 29704/IN-4310 3/29/13 Changed wingnut in blower assembly to hex nut (310-202-1-D) ccs

12 28116/IN-4201 5/29/12 Chg’d harness from 190522 to 1900089 - pg 8-6 ccs

11 27990/IN-4179 5/3/12 Added Intended Use to pg ii, updated Specs uniformity to 0.3°C from 0.2 ccs

10 27292/IN-4073 9/28/11 Switcher from 400199 to 400201 kit - pg 8-1 ccs

-- -- 7/13/10 Re-installed “Specs are based on...” on Specification page. Unintentional removal ccs

Thermo Scientificii Model 310 Series DH Incubator

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Warning If this unit is not used in the manner specified in this operating manual, the protection provided by

the equipment design may be impaired. s

Intended Use: The 310 Series Direct Heat Incubators are designed to maintain an optimal environment for

the incubation of tissue and cell samples. These models are designed to maintain temperature and Carbon

Dioxide levels as set by the operator as well as monitoring chamber relative humidity. CO2 is controlled using

either a thermo conductivity cell, or an infra-red sensor. T

The incubators are approved for general purpose use as a Microbiological Incubator (21 CFR 866.2540) and

are also approved use in assisted reproductive procedures for the incubation of ova and embryos (21 CFR

884.6120).

Non-intended Use: The 310 Series Direct Heat Incubators are not intended for use where electrical or physical

contact with the patient is established.

These incubators are not intended to be operated in potentially explosive environments and are not intended

for use with flammable materials.

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject

to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related

to the use of this manual.

Thermo Scientific Model 310 Series DH Incubator iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be

damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment

falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market

after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher

Scientific has contracted with one or more recycling/disposal companies in each EU Member State

European Country, and this product should be disposed of or recycled through them. Further

information on Thermo’s compliance with this directive, the recyclers in your country and

information on Thermo Scientific products will be available at www.thermo.com.

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Model 310 Series DH Incubator

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

1-800-438-4851 Toll Free, U.S. and Canada

1-877-213-8051 FAX

http://www.thermoscientific.com Internet Worldwide Web Home Page

service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Page

Thermo Fisher Scientific

401 Millcreek Road, Box 649

Marietta, OH 45750

Our staff can provide information on pricing and give you quotations. We can

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation or

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

International customers, please contact your local Thermo Scientific distributor.

Sales Support

Service Support

www.unitylabservices.com

Thermo Scientific Model 310 Series DH Incubator vThermo Scientific

Preface

Warranty Notes

Information You Should Know Before Requesting Warranty Service

•Locate the model and serial numbers.A serial tag is located on the unit itself.

•For equipment service or maintenance, or with technical or special application inquiries, contact Technical

Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local

distributor.

Repairs NOT Covered Under Warranty

•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°C for

temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as

a billable service at your location. Calibration after a warranty repair is covered under the warranty.

•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to

equipment materials.

•Service calls for improper installation or operating instructions. Corrections to any of the following

are billable services:

1) electrical service connection

2) tubing connections

3) gas regulators

4) gas tanks

5) unit leveling

6) room ventilation

7) adverse ambient temperature fluctuations

8) any repair external to the unit

•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper

installation.

•Repairs to parts or systems resulting from unauthorized unit modifications.

•Any labor costs other than that specified during the parts and labor warranty period, which may

include additional warranty on CO2sensors, blower motors, water jackets, etc.

Model 310 Series DH Incubator viThermo Scientific

Table of Contents

Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2

Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrating the Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrating the T/C CO2System . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Calibrating the Infrared CO2System . . . . . . . . . . . . . . . . . . . . . . . . .2-3

Calibrating Relative Humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4

Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Turn the Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Set an Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Set a Low Temp Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Enable the Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . .3-2

Set a Low CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Setting a High CO2Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3

Enable CO2Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . .3-4

Set New Zero Number for T/C CO2Sensors . . . . . . . . . . . . . . . . . .3-4

Set New Span Number for T/C CO2Sensors . . . . . . . . . . . . . . . . . .3-5

Set a Low RH Alarm Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5

Enable RH Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . . . .3-6

Enable Temp/RH to be Displayed . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6

Select Primary Tank w/ Gas Guard Option . . . . . . . . . . . . . . . . . . . .3-7

Disable the Gas Guard System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-7

Communications Address for RS485 . . . . . . . . . . . . . . . . . . . . . . . .3-8

Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Temp Control Failure Alarm TMP CNTR ERR . . . . . . . . . . . . . . .4-2

Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

CO2 SNSR ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

IR AUTOZ ERR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Section 1

Section 2

Section 3

Section 4

vii Model 310 Series DH Incubator Thermo Scientific

Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Disinfecting the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Cleaning the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Cleaning the Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Cleaning the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Reversing the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

HEPA Filter (Factory Installed Option) . . . . . . . . . . . . . . . . . . . . . . .5-8

Replacing the Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8

Major Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9

Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9

Replacing the Sample Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10

Factory Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Connect Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Connect RS485 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Connecting the Analog Output Boards . . . . . . . . . . . . . . . . . . . . . . .6-2

CO2Gas Guard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Humidity Readout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6

Uninterruptible Power Supply Connections . . . . . . . . . . . . . . . . . . .6-7

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Table of Contents

Section 5

Section 6

Section 8

Section 7

Section 9

Section 10

Model 310 Series DH Incubator 1-1Thermo Scientific

Section 1 Installation and Start-Up

Incubator components are described below.

Figure 1-1. Model 310 Series Direct Heat Incubator

• Outer Door - Reversible to opposite swing, see Section 5

• Inner Door - Reversible to opposite swing, see Section 5

• Chamber Gas Sample Port - Used for sampling chamber CO2content

using a FYRITE or similar instrument.

• Main Power Switch

• Control Panel - Keypad, Displays and indicators (See Figure 1-2)

• Leveling Legs - Used to level the unit

Note The incubators are stackable. Information follows. s

1-2 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-up

Silence - Silences the audible alarm.

Alarm Indicator - Light pulses on/off during an alarm condition in the

cabinet.

Mode Select Switch - Used to select Run, Setpoints, Calibration and

System Configuration Modes.

Message Center - Displays system status.

Mode Select Indicators -

Run: Run Menu

Set: Set Points Menu

Cal: Calibrate Menu

Config: Configuration Menu

Up and Down Arrows - Increases or decreases the number values, toggles

between choices.

Enter - Stores the value into computer memory.

Heat Indicator - Lights when power is applied to the heaters.

Temp Display - Program to display temperature continuously, RH con-

tinuously (with RH option), or toggle between temperature and humid-

ity (with RH option). See Section 3, Configuration.

Scroll for Parameters Arrows - Moves the operator through the choices

of the selected mode.

CO2Inject Indicator - Lights when CO2is being injected into the incu-

bator.

% CO2 Display - Displays CO2percentage continuously.

Control Panel Keys,

Displays & Indicators

Figure 1-2. Control Panel

Model 310 Series DH Incubator 1-3Thermo Scientific

Section 1

Installation and Start-up

The Model 310 Series direct heat incubator has four basic modes which

allow incubator setup: Run, Setpoints, Calibration and System

Configuration.

Run is the default mode which the incubator will normally be in during

operation.

Set is used to enter system setpoints for incubator operation.

Calibration is used to calibrate various system parameters.

Configuration allows for custom setup of various options.

The chart below shows the selections under each of the modes.

1T/C units only Base Unit Displays

2IR units only Option Displays

*T/C units only

Scroll for Parameters Arrows: Steps the operator through the parameters

of SET, CAL and CONFIG Modes. The right arrow goes to the next

parameter, the left arrow returns to the previous parameter.

Up Arrow: Increases or toggles the parameter value that has been selected

in the SET, CAL, and CONFIG Modes.

Enter: Must press Enter key to save to memory all changed values.

Down Arrow: Decreases or toggles the parameter values that have been

selected in the SET, CAL and CONFIG Modes.

Silence Key: Press to silence the audible alarm. See Section 4 for alarm

ringback times.

Keypad Operation

RUN SETPOINTCALIBRATION CONFIGURATION

Default Mode Temperature Temp Offset Audible

Overtemp CO2Cal1Access Code

CO2IR Cal2Temp Lo Alarm

RH Cal Temp Relay

CO2 Lo Alarm

CO2 Hi Alarm

CO2 Relay

CO2 Z & S #'s *

RH Lo Alarm

RH Relay

Display Temp

Display Rh

Tank Select

Gas Guard

RS485 Address

Message Center: Displays the system status (Mode) at all times. Displays

SYSTEM OK during normal operation, or alarm messages if the system

detects an alarm condition. See Section 4, Alarms.

Upper and Lower Displays: These 7 segment displays vary depending

upon the options present and the configuration chosen. The upper dis-

play can display temp or RH, or toggle between them. The bottom dis-

play shows CO2continuously.

Warning Single and stacked units must be installed against a wall or

similar structure. Maintain a three-inch clearance behind the incubator for

electrical and gas hook-ups. s

1. Locate the unit on a firm level surface capable of supporting the unit’s

weight of 205 lbs.

2. Locate the unit away from doors and windows and heating and air

conditioning ducts.

3. Lift the unit only by the sides of the cabinet base . Do not attempt to

lift it by the front and back . This places stress on the outer door

hinges.

Caution When stacking incubators, the direct heat incubator must be the

top unit. Never stack a water-jacketed incubator on top of a Model 310

Series unit. s

Warning With incubators in a stacked configuration, do not leave both

exterior doors open at the same time. s

Warning If the units have been in operation, shut them both off and

disconnect from the power source before beginning any service work. s

Note Two stacking brackets (shown at right) are included

in the parts bag shipped with each incubator. s

1-4 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Displays

Installing the

Incubator

Stacking the Incubators

Figure 1-3.

Stacking Brackets

1. Remove and discard the slotted head

screws on the top of the bottom incubator

(Figure 1-4). Replace with 5/16 HH bolts

and flatwashers included in the stacking

kit. Do not tighten.

2. Remove the kickplate from below the door on the top incubator

(Figure 1-5). Two Phillips screws secure the plate. Note the notches at

the base of the incubator frame.

3. Disconnect the door cable from the connector behind the plate. Lift

the door off the hinge pins and set it aside. The hinge pins are not

attached to the brackets and may fall out when the door is lifted off.

4. Unscrew and remove the leveling feet from the top unit and lift it onto

the bottom unit, offsetting the base of the top unit approximately 2-3

inches behind the screws identified by the arrows in Figure 1-4.

Warning This incubator weighs 205 lbs. Have sufficient personnel

available when lifting. Lift the unit only by the sides of the cabinet base.

Do not attempt to lift it by the front and back as this places stress on the

outer door hinge. s

5. Align the sides of the top unit with the bottom unit and slide the top

unit forward until the notches in the base of the top unit align with

the bolts in the top of the bottom unit. The flatwashers should slide

over the base frame. Tighten bolts.

6. Remove the four nylon plugs on the lower back of the upper incubator.

7. Insert the stacking brackets into the slots on the back of the control

panel of the bottom unit as shown in Figure 1-6.

Model 310 Series DH Incubator 1-5Thermo Scientific

Section 1

Installation and Start-Up

Stacking (continued)

Figure 1-4. Slotted Head Screws Figure 1-5. Align

Figure 1-6. Brackets Installed

8. Align the slots in the brackets with the mounting holes on the back of

the top incubator. Secure the brackets with the screws provided in the

stacking kit.

9. Make sure the hinge pins are in the hinge brackets. Slide the door onto

the hinge pins.

10. Secure the door heater cable to the connector below the door and

replace the kick plate on the top unit, taking care not to crimp cable.

11. The stacked incubators are ready for service.

Remove vinyl from shelf channels, duct sheets, and air duct, if present.

Using a suitable laboratory disinfectant, disinfect all interior surfaces

including shelves and shelf supports, door gaskets, blower wheel and CO2

sensor. Refer to Section 5.

Caution Before using any cleaning or decontamination method except

those recommended by the manufacturer, users should check with the

manufacturer that the proposed method will not damage the equipment.

Accidental spills of hazardous materials on or inside this unit are the

responsibility of the user. s

1. Install the large sheet metal shelf supports with the tabs facing into the

center of the chamber with their slots up. There are no right side or

left side supports. Simply turn one of them to fit the opposite side. Tilt

the shelf supports as they are placed in the chamber so the tops fit into

the top air duct, then guide them into the vertical position. Figure 1-7

shows the support as it would be oriented for right side of chamber.

1-6 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Preliminary Cleaning and

Disinfecting

Installing the Shelves

Figure 1-7. Shelf Channels and Ductwork

Stacking (continued)

2. Note there is no difference in the left side and right side shelf channels.

3. Install the shelf channels by placing the channel’s rear slot over the

appropriate rear tab on the shelf support. Pull the shelf channel

forward and engage the channel’s front slot into the shelf support’s

appropriate forward tab. Refer to Figure 1-8.

Locate the opening in the top left corner on the inside of the chamber.

Remove the tape covering the opening on the outside of the unit. Locate

the stopper with filter in the hardware bag. Install the assembly in the

opening inside the chamber. See Figure 1-9.

Figure 1-9. Filter and Sensor Locations

Caution Be careful when handling the filter. The media can be damaged if

it is mishandled.

To avoid damage to the incubator, do not operate the unit without the

HEPA filter in place. s

1. Remove the filter from the shipping box.

2. Remove the plastic coating from the filter, using caution not to touch

the filter media.

3. Install the filter as shown in Figure 1-9. Refer to Section 5 for HEPA

filter maintenance.

Model 310 Series DH Incubator 1-7Thermo Scientific

Section 1

Installation and Start-Up

Figure 1-8. Channel Ends

HEPA Filter

Sample Air Filter

Temperature

sensor

Access

port filter

Installing (optional) HEPA

Filter

Installing Access Port

Filter

Installing the Shelves

(continued)

Leveling the Unit

1. Remove the filter from the shipping bag.

2. Install the air sample filter assembly to the black hose barb behind the

top duct.

3. Insert the other end of the filter assembly into the larger hole in the air

intake to the blower. Refer to Figure 1-10.

4. Verify that the temperature sensor is installed into the smaller hole in

the intake, as in Figure 1-10.

Check for level by placing a bubble style level on one of the shelves. Turn

the hex nut on the leveler counterclockwise to lengthen the leg or

clockwise to shorten it. Level the unit front-to-back and left-to-right.

See the serial tag on the side of the unit for electrical specifications or refer

to the electrical schematics at the end of this manual.

Caution Connect the incubator to a grounded, dedicated circuit.

The power cord connector is the mains disconnect device for the

incubator. Position the incubator so the unit can be easily disconnected. s

1-8 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Figure 1-10. View of Chamber Ceiling

Installing the Air Sample

Filter

Connecting the Unit to

Electrical Power

Plug the provided power cord into the power inlet connector on the back

of the cabinet (See Figure 1-11), then into the grounded, dedicated,

electrical circuit.

Figure 1-11. Connections on Unit Back

Fill the humidity pan with sterile pure water to within 1/2” of the top.

Place the pan directly in the center of the incubator floor to ensure best

humidity and temperature response.

For applications requiring abnormally high humidity, the pan should be

placed against the left side wall of the incubator. The ductwork has been

modified to allow the pan to be placed against the wall of the incubator.

In some ambient conditions, this may cause condensation to form in the

chamber.

For best operation of the incubator, sterilized distilled, demineralized or

de-ionized water should be used in the humidity pan. Water purity should

be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range

of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88

for measuring water purity.

Distillation systems, as well as some types of reverse osmosis water purity

systems, can produce water in the quality range specified. Tap water is not

recommended as it may contain chlorine, which can deteriorate the

stainless steel. Tap water may also have a high mineral content, which

would produce a build-up of scale in the pan. High purity or ultra pure

water is not recommended as it is an extremely aggressive solvent and will

deteriorate the stainless steel. High purity water has a resistance of above

1M to 18M Ohm. Even high purity water can contain bacteria and

organic contaminants. Water should always be sterilized or treated with a

decontaminant, safe for use with stainless steel as well as safe for the

product, prior to being introduced into the humidity pan.

Model 310 Series DH Incubator 1-9Thermo Scientific

Section 1

Installation and Start-Up

Filling the Humidity Pan

Connecting the Unit to

Electrical Power (cont.)

Caution Distilled or de-ionized water used in the humidity pan must be

within a water quality resistance range of 50K to 1M Ohm/cm to protect

and prolong the life of the stainless steel. Use of chlorinated water, or

decontamination products containing chlorine, will deteriorate the stainless

steel and cause rust, voiding the warranty. s

Check the level and change the water frequently to avoid contamination.

Do not allow the water level to fluctuate significantly. “Dry-outs” will have

an adverse effect on the humidity level and CO2calibration of the T/C

units.

Warning High concentrations of CO2gas can cause asphyxiation! OSHA

Standards specify that employee exposure to carbon dioxide in any eight-

hour shift of a 40-hour work week shall not exceed the eight-hour time

weighted average of 5000 PPM (0.5% CO2). The short term exposure

limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide

monitors are recommended for confined areas where concentrations of

carbon dioxide gas can accumulate. s

Warning This incubator is designed to be operated with CO2gas only.

Connecting a flammable or toxic gas can result in a hazardous condition.

Gases other than CO2should not be connected to this equipment. CO2

gas cylinders have a UN1013 label on the cylinder and are equipped with a

CGA 320 outlet valve. Check the gas cylinder for the proper identification

labels. The CO2gas supply being connected to the incubator should be

industrial grade, 99.5% pure. Do not use CO2gas cylinders equipped with

siphon tubes. A siphon tube is used to extract liquid CO2from the

cylinder which can damage the pressure regulator. Consult with your gas

supplier to ensure that the CO2cylinder does not contain a siphon tube.

Gas cylinders should also be secured to a wall or other stationary object to

prevent them from tipping.

A two-stage CO2pressure regulator is required to be installed on the outlet

valve of the gas cylinder. Input pressure to the incubator must be

maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the

CO2control system. A single stage CO2pressure regulator will not

maintain 15 psig (103.4 kPa) to the incubator as the pressure in the CO2

cylinder decreases; therefore, a two stage regulator is recommended.

If higher purity CO2is desired inside the incubator (greater than 99.5%

pure), the pressure regulator should be constructed with a stainless steel

diaphragm, along with specifying the purity of the CO2from the gas

supplier. Follow the manufacturer’s instructions to ensure proper and safe

installation of the pressure regulator on the gas cylinder.

Consult your facility safety officer to ensure that the equipment is installed

in accordance with the codes and regulations applicable in your area. s

1-10 Model 310 Series DH Incubator Thermo Scientific

Section 1

Installation and Start-Up

Connecting the CO2Gas

Supply

Filling the Humidity Pan

(cont.)

The CO2gas supply being connected should be industrial grade 99.5%

pure and should not contain siphon tubes. Install a two-stage pressure

regulator at the cylinder outlet. The high pressure gauge at the tank should

have 0-2000 psig range. The low pressure gauge, at the incubator inlet,

should have a 0-30 psig range. Input pressure to the incubator must be

maintained at 15 psig (103.4 kPa).

The incubator has serrated fittings on the back of the cabinet to connect

the gas supply. Refer to Figure 1-11. The fitting is labeled CO2 Inlet #1

Tank. Make sure that the connections are secured with clamps. Check all

fittings for leaks.

For units having the CO2Gas Guard option, refer to Section 6.

With the incubator properly installed and connected to power, the

humidity pan filled, and the unit connected to gas supplies, system

setpoints can be entered. The following setpoints can be entered in Set

mode: Temperature, Overtemperature and CO2. To enter Set mode, press

the Mode key until the Set indicator lights. Press the right and/or left

arrow keys until the proper parameter appears in the message center. See

Chart 1-1 for more detail.

Model 310 Series incubators have an operating temperature range of 10°C

to 50°C, depending on ambient temperature. The incubator is shipped

from the factory with a temperature setpoint of 10°C. At this setting, all

heaters are turned off. To change the operating temperature setpoint:

1. Press the Mode key until the Set indicator lights.

2. Press the right arrow until “Temp XX.X” is displayed in the message

center.

3. Press the up/down arrow key until the desired temperature setpoint is

displayed.

4. Press Enter to save the setpoint.

5. Press the Mode key until the Run indicator lights for Run mode or

press the right/left arrow keys to go to next/previous parameter.

Model 310 Series DH Incubator 1-11Thermo Scientific

Section 1

Installation and Start-Up

Incubator Start-Up

Setting the Operating

Temperature

Connecting the CO2gas

Supply (continued)

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