Hygiena AOAC-RI Operating instructions

Brand: Hygiena

Model: AOAC-RI

Type: Operating instructions

Hygiena AOAC-RI is a revolutionary device that provides accurate, reliable, and validated quantification of Salmonella in poultry, beef, pork, and their trims, enabling faster data-driven decisions for improved food safety. It utilizes the SalQuant™ methodology and advanced BAX® System Real-Time PCR Assay technology to deliver precise results, aiding in reducing product hold times, verifying corrective actions, monitoring process control, and promoting efficient diversion decisions.

INTRODUCTION:

Process control and final product decisions based only

on prevalence have shown limitations in reducing

consumer risk. Therefore, adoption of validated

quantification methodologies with low error and wide

enumerable ranges should be utilized to make data-

driven food safety decisions.

Savannah F. Applegate1, Rossy L. Bueno Lopez2, April K. Englishbey1, Nisha Corrigan1,

Stacy K. Stoltenberg1, Tyler P. Stephens1, and Marcos X. Sanchez-Plata2

1Hygiena™, 2 Boulden Circle, New Castle, DE 19720

2Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409

Twenty samples per matrix were

divided into 4 inoculation levels with

Salmonella ATCC strains at low:

0.0–1.0 Log CFU/g(mL), medium:

1.5–2.5 Log CFU/g(mL), high: 2.5–

3.5 Log CFU/g(mL), or uninoculated

as negative controls, then cold-

stressed for 48-72 hours. After the

stress period, sample processing

followed the Hygiena Pathogen

Quantification Procedures Manual

with incubation at 42 ˚C for 6 or 8

hours for SalQuant with reference

method performed in parallel.

Candidate results were compared

to the reference results with

statistical equivalence within 90%

confidence intervals and mean

difference of ± 0.50 Log10

CFU/g(mL) utilizing the AOAC-RI

quantitative worksheet.

METHODOLOGY: RESULTS:

The BAX SalQuant candidate method was

comparable to the USDA-FSIS MLG MPN

reference method at all inoculation levels for

poultry rinsates, ground beef, ground pork,

beef trim, pork trim, and MicroTally for beef

and pork trim.

SIGNIFICANCE:

PURPOSE:

The aim of this certification study was to compare the

performance of the candidate method, BAX®System

Real-Time PCR Assay for Salmonella (KIT2006)

utilizing the SalQuant™methodology, to the USDA-

FSIS MLG Most Probable Number (MPN) quantitative

reference method.

AOAC-RI Validation of Hygiena™BAX®System SalQuant™Methods

for Poultry Rinsates, Ground Beef and Pork, Beef and Pork Trim,

and MicroTally™Manual Sampling Devices on Beef and Pork Trim

One Health Diagnostics

REGISTERED TRADEMARKS /

GLOBAL CERTIFICATIONS:

BAX®System Real-Time PCR Assay for Salmonella AOAC-RI Performance

Tested Methods℠Certification Number 081201

MicroTally™Manual Sample Device is a trademark of MicroTally.

Low Med High

SalQuant 0.86 2.10 3.23

MLG MPN 0.97 2.37 3.59

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Ground Beef

Low Med High

SalQuant 0.99 2.28 3.32

MLG MPN 0.70 2.19 3.61

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/mL

AOAC -Poultry Rinsates

This certification provides the

poultry, beef, and pork industries

with an accurate, reliable, and

validated quantification tool to

reduce product hold-times, verify

corrective actions, monitor process

control, and promote faster data-

driven diversion decisions which

ultimately reduces consumer risk

in animal protein products.

Low Med High

SalQuant 0.70 1.59 3.39

MLG MPN 0.71 1.72 3.53

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Ground Pork

Low Med High

SalQuant 0.53 2.21 3.41

MLG MPN 0.69 2.48 3.49

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Pork Trim

Low Med High

SalQuant 0.70 1.79 3.51

MLG MPN 0.46 2.18 3.12

-1.00

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Beef Trim

Low Med High

SalQuant 0.65 2.13 3.22

MLG MPN 0.60 1.80 3.16

-1.00

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Pork Trim MicroTally

Low Med High

SalQuant 0.75 2.01 3.26

MLG MPN 0.63 2.25 3.61

0.00

1.00

2.00

3.00

4.00

5.00

Log10 CFU (MPN)/g

AOAC -Beef Trim MicroTally

Hygiena AOAC-RI Operating instructions

Hygiena AOAC-RI Operating instructions

Hygiena AOAC-RI Operating instructions

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