Qubit™ 4 Fluorometer User Guide
1
Contents
About this guide ............................................................................................................ 3
1. Product information ................................................................................................ 5
Product contents .............................................................................................................................................. 5
Product description ......................................................................................................................................... 6
Instrument exterior components ................................................................................................................... 7
Product specifications ..................................................................................................................................... 8
2. Getting started ........................................................................................................ 9
Set up the Qubit™ 4 Fluorometer ................................................................................................................... 9
Optional: Configure instrument settings ..................................................................................................... 11
Guidelines for using the Qubit™ 4 Fluorometer ........................................................................................ 16
3. Perform assays ..................................................................................................... 17
Overview ........................................................................................................................................................ 17
Optional: Use Reagent Calculator to prepare Qubit™ Working Solution ............................................... 18
Calibrate assays on the Qubit™ 4 Fluorometer .......................................................................................... 20
Read samples ................................................................................................................................................. 24
Results ............................................................................................................................................................. 26
Fluorometer mode ......................................................................................................................................... 29
Ion Sphere™ Assay ......................................................................................................................................... 31
4. Manage data .......................................................................................................... 32
Introduction ................................................................................................................................................... 32
View detailed sample data ........................................................................................................................... 32
Rename data files........................................................................................................................................... 35
Export data ..................................................................................................................................................... 36
Delete data ...................................................................................................................................................... 40
5. Manage, import, and create assays ....................................................................... 41
Overview ........................................................................................................................................................ 41
Manage assays ............................................................................................................................................... 41
Show/Hide assays ........................................................................................................................................ 42
Move assays ................................................................................................................................................... 44
Import assays ................................................................................................................................................. 46
Create your own assay for the Qubit™ 4 Fluorometer .............................................................................. 51
6. Instrument maintenance ....................................................................................... 52
Maintenance and cleaning............................................................................................................................ 52
Software updates ........................................................................................................................................... 53
System verification test ................................................................................................................................. 55
Appendix A: Troubleshooting ...................................................................................... 57
Troubleshooting ............................................................................................................................................. 57