Thermo Fisher Scientific Shandon M-1 Embedding Matrix Operating instructions

Type
Operating instructions
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus Drive, Kalamazoo, MI 49008
Phone: 1 (800) 522-7270
Phone: + 1 (269) 544-5600
www.thermoscientific.com/pathology
Anatomical Pathology
©2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
Thermo Shandon Limited
Subsidiary of Thermo Fisher Scientific
Tudor Road, Manor Park,
Runcorn, Cheshire WA7 1TA, UK
Phone: +44 (0) 1928 534 050
Fax: + 44 (0) 1928 534 049
Thermo Scientic Shandon M-1 Embedding Matrix Instructions for Use
Thermo Scientific Shandon M-1 Embedding Matrix is an outstanding embedding medium used to support
tissue for frozen sectioning at temperatures between -12° to -14° C. It forms a firm bond with the specimen
holder that secures tissue during cryotomy. M-1 Embedding Matrix freezes quickly, sections smoothly and
dissolves in water without residue.
Instructions For Use
1. Cover the surface of the specimen holder with a thick layer of M-1 Embedding Matrix.
2. Position and orient tissue on holder and press lightly to eliminate air bubbles beneath the tissue.
3. Apply additional M-1 Embedding Matrix until the tissue is surrounded.
4. Freeze tissue to holder in usual manner until tissue and medium are firm. The color of the medium will
lighten considerably but will still be easily identifiable.
5. Section specimen in usual manner.
6. No special procedure is required to remove M-1 Embedding Matrix from sections; it will dissolve in alcohol
or aqueous solutions during the staining procedure.
7. To remove specimen from holder for further processing, dissolve medium and immediately place specimen
in fixative.
8. To avoid vacuole formation within the cells (sometimes referred to as “freeze artifact”) it is best to freeze
tissues as rapidly as possible. Tissues allowed to freeze slowly have sufficient time for the formation of ice
crystals within the cells. These ice crystals expand inside the cells. When the ice crystals melt during stain-
ing, large holes or vacuoles remain inside the cells.
Warnings and Precautions
See Material Safety Data Sheets for warnings and precautions, as well as R & S code definitions.
See container label for warnings and precautions.
Order Information
Product Size Qty. REF
M-1 Embedding Matrix 180mL Ea. 1310
238164 R1/13
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Thermo Fisher Scientific Shandon M-1 Embedding Matrix Operating instructions

Type
Operating instructions

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