2. Thermo Fisher Scientific
  3. Procise® PTH Chromatography Troubleshooting Tree

Thermo Fisher Scientific Procise® PTH Chromatography Troubleshooting Tree User guide

  • Hello! I've analyzed the provided document, which is a user manual for Procise PTH Chromatography. It's a troubleshooting guide that helps resolve issues like uneven retention times, poor peak resolution, and flat baselines. I'm ready to answer any questions you might have about the manual or the device.
  • What should I do if my baseline is flat?
    What to do in case of poor peak resolution?
    What if ILE/LYS/LEU peaks are squeezed together?
    How to fix ILE/LYS poorly resolved peaks?
Procise® PTH Chromatography Troubleshooting Tree
Chromatography
Problem
Peak
Resolution
sharp
Peak resolution
poor or peaks
broad, blunt
Baseline Flat
Check detector
(785/S200) for
deuterium lamp
strength
Lamp OK
Adjacent peaks
poorly resolved Peak heights
uneven
ILE - LYS -
LEU poorly
separated
HIS/ALA and/or
ARG/TYR
poorly resolved
Check cable to
Procise®
sequencer;
tighten or replace
Lamp weak
(-0.19 or less) or
dead (+0.55);
replace
ILE/LYS poorly
resolved
LYS/LEU poorly
resolved
Decrease %B at
18.0 min. (HT) or
22.0 min. (cLC)
Increase %B at
18.0 min. (HT) or
22.0 min. (cLC)
Increase %B at
0.4 min. (HT) up
to 18%, or
increase Premix
concentration
GLN/THR or
MET/VAL poorly
resolved
Replace PTH
column
Replace Solvent
A3
Replace R5 PTH
standards
ILE/LYS/LEU too
squeezed together
Retention times
shift/baseline
uneven
Replace pump
seals in 140C/D
119GU02-01
/