Thermo Fisher Scientific Procise® PTH Chromatography Troubleshooting Tree User guide

Brand
Thermo Fisher Scientific
Model
Procise® PTH Chromatography Troubleshooting Tree
Type
User guide
Procise® PTH Chromatography Troubleshooting Tree
Chromatography
Problem
Peak
Resolution
sharp
Peak resolution
poor or peaks
broad, blunt
Baseline Flat
Check detector
(785/S200) for
deuterium lamp
strength
Lamp OK
Adjacent peaks
poorly resolved Peak heights
uneven
ILE - LYS -
LEU poorly
separated
HIS/ALA and/or
ARG/TYR
poorly resolved
Check cable to
Procise®
sequencer;
tighten or replace
Lamp weak
(-0.19 or less) or
dead (+0.55);
replace
ILE/LYS poorly
resolved
LYS/LEU poorly
resolved
Decrease %B at
18.0 min. (HT) or
22.0 min. (cLC)
Increase %B at
18.0 min. (HT) or
22.0 min. (cLC)
Increase %B at
0.4 min. (HT) up
to 18%, or
increase Premix
concentration
GLN/THR or
MET/VAL poorly
resolved
Replace PTH
column
Replace Solvent
A3
Replace R5 PTH
standards
ILE/LYS/LEU too
squeezed together
Retention times
shift/baseline
uneven
Replace pump
seals in 140C/D
119GU02-01
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Thermo Fisher Scientific Procise® PTH Chromatography Troubleshooting Tree User guide

Brand
Thermo Fisher Scientific
Model
Procise® PTH Chromatography Troubleshooting Tree
Type
User guide
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