Thermo Fisher Scientific MicroSeq® ID Analysis Software Quick start guide

MicroSeq® ID Analysis

Software

Version 1.0

Getting Started Guide

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For Research Use Only. Not for use in diagnostic procedures.

Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that

may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall

Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use

of this document.

NOTICE TO PURCHASER: DISCLAIMER OF LICENSE

Purchase of this software product alone does not imply any license under any process, instrument or other apparatus, system,

composition, reagent or kit rights under patent claims owned or otherwise controlled by Applera Corporation, either expressly or by

estoppel.

NOTICE TO PURCHASER:

PLEASE REFER TO THE MICROSEQ® IDENTIFICATION KIT PROTOCOL FOR LIMITED LABEL LICENSE OR

DISCLAIMER INFORMATION

TRADEMARKS:

ABI PRISM, Applied Biosystems, BigDye, and MicroSeq are registered trademarks of Applera Corporation or its subsidiaries in the

U.S. and/or certain other countries.

AB (Design), Applera, and POP-6 are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other

countries.

All other trademarks are the sole property of their respective owners.

Part Number 4345867 Rev. A

08/2003

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Contents

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide iii

Preface

How to Use This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Purpose of This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Audience . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Assumptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Text Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

User Attention Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

How to Obtain More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

Send Us Your Comments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

How to Obtain Services and Support . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Chapter 1 Software Basics

Start Here: Getting the Most out of This Guide . . . . . . . . . . . . . . . . . . 1-2

Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

What is the MicroSeq ID Analysis Software? . . . . . . . . . . . . . . . . . . . . 1-2

User Groups and Privileges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

User Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Tutorial Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5

Software Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

Options Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6

MicroSeqID Manager Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7

Project Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7

Software Toolbars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

General Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

Analysis Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9

Display Toolbar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10

Terms Used in This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-11

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iv MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

File-Naming Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13

For More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13

Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13

Context-Sensitive Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-13

Chapter 2 Administrator: Set Up the Software

Review Requirements and Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

User Privileges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Administrator Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Register the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

License and Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Register the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Check Hardware and Software Requirements . . . . . . . . . . . . . . . . . . . 2-4

Minimum System Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

Hard Drive Partitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

Install the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

Prepare for Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

Install the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6

Start the Software for the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8

Set Up General Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10

Set Up User Accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12

Create New Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-12

Change User Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-13

Import/Export User Accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14

Set Up Authentication and Auditing . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15

Set Up Authentication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-15

Set Up the Audit Trail . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-16

Import/Export Audit Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-17

Chapter 3 Scientist: Set Up Analysis Information

Review Requirements and Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

User Privileges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

User Accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Scientist Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Log In . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

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MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide v

Create a Custom Library . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Create a Master Analysis Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

Use the MicroSeqID Manager . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

Open a New Master Analysis Protocol . . . . . . . . . . . . . . . . . . . . . . 3-8

Specify Basecalling Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10

Specify Mixed Bases Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11

Specify Clear Range Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12

Specify Filter Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13

Chapter 4 Analyst: Set Up a Project

Review Requirements and Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

User Privileges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

User Accounts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Analysis Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Analyst Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Log In . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Customize the Display Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3

Create a Project . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6

Select Project Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6

Create a New Specimen and Import Sample Files . . . . . . . . . . . . . 4-8

View the Project Setup Summary . . . . . . . . . . . . . . . . . . . . . . . . . 4-11

Analyze the Project Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-12

Options for Analyzing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-12

Finish and Analyze the Project Data . . . . . . . . . . . . . . . . . . . . . . . 4-13

Evaluate Results: Analysis QC Report . . . . . . . . . . . . . . . . . . . . . . . . 4-15

View the Analysis QC Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-15

Interpret the Specimen Analysis Section . . . . . . . . . . . . . . . . . . . 4-16

Interpret the Sample Analysis Section . . . . . . . . . . . . . . . . . . . . . 4-18

Evaluate Results: Library Search Report . . . . . . . . . . . . . . . . . . . . . . 4-20

View the Library Search Report . . . . . . . . . . . . . . . . . . . . . . . . . . 4-20

Interpret the Hit List Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-21

Interpret the Concise Alignment Section . . . . . . . . . . . . . . . . . . . 4-22

Interpret the Phylogenetic Tree Section . . . . . . . . . . . . . . . . . . . . 4-22

Edit Data in the Analyzed Project . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24

When to Edit Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24

How to Edit Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24

Edit a Consensus Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24

Edit the Sample File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-26

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vi MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

Reanalyze and Save the Project . . . . . . . . . . . . . . . . . . . . . . . . . 4-28

Export and Print Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-28

Customize the Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-28

Export the Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-30

Print the Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-30

Appendix A Software Warranty Information

Computer Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2

Limited Product Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2

Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2

Warranty Period Effective Date . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Warranty Claims . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Warranty Exceptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Warranty Limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

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MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide vii

Preface

How to Use This Guide

Purpose of This

Guide

The Applied Biosystems MicroSeq® ID Analysis Software Version

1.0 Getting Started Guide is an installation guide and tutorial. It

provides:

• Installation instructions to help the Administrator set up the

MicroSeq® ID Analysis software.

• Brief, step-by-step tasks to help users quickly learn the

MicroSeq ID Analysis software. The tasks reference tutorial

data that is supplied on the software CD-ROM.

Audience This guide is intended for new users of the MicroSeq ID Analysis

software. There are three user groups: Administrator, Scientist, and

Analyst. (For more information on user groups, see page 1-3.)

Assumptions This guide assumes that you have:

• A working knowledge of the Microsoft® Windows® operating

system

• Access to the MicroSeq ID Analysis software Online Help

Text Conventions This guide uses the following conventions:

•Bold indicates user action. For example:

Ty pe 0, then press Enter for each of the remaining fields.

•Italic text indicates variables and new or important words. Italic

text is also used for emphasis. For example:

Before analyzing, always prepare fresh matrix.

• A right arrow bracket (>) separates successive commands you

select from a drop-down or shortcut menu. For example:

Select File > Open > Spot Set.

Right-click the sample row, then select View Filter > View All

Runs.

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Preface

viii MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

User Attention

Words

Two user attention words appear in Applied Biosystems user

documentation. Each word implies a particular level of observation

or action as described below:

Note: Provides information that may be of interest or help but is not

critical to the use of the product.

IMPORTANT! Provides information that is necessary for proper

instrument operation, accurate chemistry kit use, or safe use of a

chemical.

Examples of the user attention words appear below:

Note: The size of the column affects the run time.

Note: The Calibrate function is also available in the Control Console.

IMPORTANT! To verify your client connection to the database, you

need a valid Oracle user ID and password.

IMPORTANT! You must create a separate Sample Entry Spreadsheet

for each 96-well microtiter plate.

How to Obtain More Information

The following related documents are available:

•MicroSeq® ID Analysis Software Version 1.0 Online Help –

Describes the MicroSeq ID Analysis software and provides

procedures for common tasks.

The Online Help is supplied as part of the MicroSeq ID Analysis

software. When the software is installed and running, press F1,

or select Help > Contents & Index.

•MicroSeq® ID Analysis Software Version 1.0 Quick Reference

Card – Provides the Analyst with a brief description of the tasks

required to set up a project in the MicroSeq ID Analysis

software.

A portable document format (PDF) version of the Quick

Reference Card is provided on the software CD-ROM.

Note: For additional documentation, see “How to Obtain Services

and Support” on page ix.

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How to Obtain Services and Support

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide ix

Send Us Your

Comments

Applied Biosystems welcomes your comments and suggestions for

improving its user documents. You can e-mail your comments to:

[email protected]

How to Obtain Services and Support

For the latest services and support information for all locations, go to

http://www.appliedbiosystems.com, then click the link for Services

and Support.

At the Services and Support page, you can:

• Download PDF documents

• Obtain information about customer training

• Download software updates and patches

In addition, the Services and Support page provides access to

worldwide telephone and fax numbers to contact Applied Biosystems

Technical Support and Sales facilities.

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Preface

xMicroSeq

® ID Analysis Software Version 1.0 Getting Started Guide

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MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide 1-1

1

Software Basics 1

This chapter covers:

Start Here: Getting the Most out of This Guide . . . . . . . . . . . . . . .1-2

What is the MicroSeq ID Analysis Software?. . . . . . . . . . . . . . . . .1-2

User Groups and Privileges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

User Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Tutorial Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Software Structure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Software Toolbars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8

Terms Used in This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11

File-Naming Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13

For More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13

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Chapter 1 Software Basics

1-2 MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

Start Here: Getting the Most out of This Guide

The Applied Biosystems MicroSeq® ID Analysis Software Version

1.0 Getting Started Guide is an installation guide and tutorial. It

provides:

• Installation instructions to help the Administrator set up the

MicroSeq® ID Analysis software.

• Brief, step-by-step tasks to help users quickly learn the

MicroSeq ID Analysis software. The tasks reference tutorial

data that is supplied on the software CD-ROM.

To get the most out of this Getting Started Guide, we recommend

proceeding as follows:

Step 1 Read this “Software Basics” chapter.

Step 2 Determine which user group you belong to. See “User Groups and

Privileges” on page 1-3.

Step 3 Work through the tutorial tasks appropriate for your user group. See

“User Workflow” on page 1-4.

What is the MicroSeq ID Analysis Software?

MicroSeq® ID Analysis software version 1.0 is a tool for microbial

identification of bacteria and fungi. The software analyzes data

generated using a MicroSeq®chemistry kit and an ABI PRISM®

capillary-based genetic analyzer.

The MicroSeq ID Analysis software:

1. Compares the sequence of unknown isolate data to the

sequences of known bacteria or fungi stored in a library.

2. Generates a final identification list of organisms that are the

closest matches to the unknown.

3. Reports the percent similarity that reflects how closely the

unknown isolate matches the library sequence.

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User Groups and Privileges

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide 1-3

User Groups and Privileges

There are three user groups defined in the software: Administrator,

Scientist, and Analyst. Each of these user groups has the privileges

listed in the table below.

User Group Privileges

Administrator • Install and start the software

• Set up defaults

• Set up users

• Set up auditing

• All Scientist privileges

• All Analyst privileges

Scientist • Create libraries

• Create analysis protocols

• All Analyst privileges

Analyst • Create a project

• Analyze the project

• Evaluate the results

• Edit data in the analyzed project

• Export or print reports

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Chapter 1 Software Basics

1-4 MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

User Workflow

The workflow below provides an overview of the tasks presented in

this Getting Started Guide. The tasks reference tutorial data (see

page 1-5) that is supplied on the software CD-ROM.

IMPORTANT! Each task should be performed in the order given by

the specified user group. The Administrator and Scientist must

perform their tasks before the Analyst can set up a project.

See

Ch. 3

Scientist:

Set up reference

data

Create libraries.

Create analysis protocols.

Create a project.

See

Ch. 4

Analyst:

Set up a project

Analyze the project.

Evaluate the results.

Edit data in the analyzed

project.

Export or print reports.

Install and start the software.

See

Ch. 2

Administrator:

Set up the

software

Set up defaults.

Set up users.

Set up auditing.

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Tutorial Data

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide 1-5

Tutorial Data

When you perform the tasks in this Getting Started Guide, you will

use tutorial data that is supplied on the MicroSeq ID Analysis

software CD-ROM. The tutorial data is in the following installation

drive location:

drive letter\AppliedBiosystems\MicroSeqID\Tutorial Data\

Bacterial500Samples

Table 1-1 defines the two type of files provided and their folder

names, as well as the specific file names and extensions.

Note: The tutorial data was electrophoresed on an ABI PRISM® 3100

Genetic Analyzer running POP-6™ polymer on a 50-cm array using

the standard run (SR) protocol. The unknown isolates were

sequenced with BigDye® Terminator v1.1 chemistry.

Table 1-1 Tutorial data referenced in this guide

File Type Folder Name File Name and Extension

Library Bacterial500CustomLibrary 500KitCustomLibrary.fsta

Sample file 500EColi MicroSeq041703_C03_H_5F_05.ab1

MicroSeq041703_D03_H_535R_07.ab1

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Chapter 1 Software Basics

1-6 MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

Software Structure

To perform the tasks in this Getting Started Guide, you will access

three windows from the MicroSeq ID Analysis software main

window:

• MicroSeqID Manager window

• Options window

•Project window

Options Window Use the Options window to create and/or edit:

• General defaults, which include

– Displaying reports after analysis

– Exporting reports after analysis

– Specifying a directory to which reports are automatically

exported

• User accounts

• Authentication and audit trails

Options Window

Analysis QC

Report

Report Manager

Project Window

MicroSeq ID Analysis Software: Main Window

View project

data

View, export, or

print results

Edit and

apply to project

Create and edit

settings

Library Search

Report

Audit Trail Report

Sample analysis

protocol

Sample Manager

General defaults

User accounts

Authentication

and audit trails

MicroSeqID Manager

Manage analysis

information

Projects

Libraries

Master analysis

protocols

Project View

Specimen View

Sample View

Access the Options window

by clicking

Tools >

Options

.

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Software Structure

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide 1-7

MicroSeqID

Manager Window

Use the MicroSeqID Manager window to enter and manage the

information necessary to perform analyses on your projects:

• Edit, delete, import, and export projects

• Create, edit, delete, import, and export libraries

• Create, edit, delete, import, and export master analysis protocols

Project Window Use the Project window to:

• View, export, or print project data (Project View, Specimen

View, or Sample View)

• Access the Sample Manager to edit sample file information

• Access the Report Manager to view, export, or print project

analysis results

Access the MicroSeqID

Manager window by

clicking

Tools >

MicroSeqID Manager

.

Access the Project window

by clicking

File > Open

Project

and selecting an

existing project.

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Chapter 1 Software Basics

1-8 MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide

Software Toolbars

The MicroSeq ID Analysis software toolbars display buttons for

software functions that you are likely to use often. Refer to the

following diagrams (pages 1-8 to 1-10) for the names, descriptions,

and keyboard shortcuts for each button.

General Toolbar The General toolbar contains general processing tools for creating

projects.

New Project

Ctrl+N

Creates a

new project

Print

Ctrl+P

Import Samples

To Project

Ctrl+M

Find Again

F3

Finds a selected

sequence again

Delete

Deletes a

selected

object

Open Project

Ctrl+O

Opens a window to

select and open a project

MicroSeqID Manager

Opens the MicroSeqID

Manager window

New Specimen

Opens a new

specimen

Find

Ctrl+F

Finds a selected

string/IUB code

in a sequence

Undo Base

Change

Ctrl+Z

Save Project

Ctrl+S

Saves a

modified project

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Software Toolbars

MicroSeq® ID Analysis Software Version 1.0 Getting Started Guide 1-9

Analysis Toolbar The Analysis toolbar contains analysis tools for the projects you

create.

A

nalyze Samples

Ctrl+R

Sample Manager

Opens the Sample

Manager window

Report Manager

Ctrl+1

Opens the Report

Manager window

Analysis Settings

Ctrl+B

When a sample file is

selected, displays the

Analysis Protocol settings

When a project is selected,

displays the Project

Analysis Settings

MyScience

Opens your Applied

Biosystems MyScience

web page.

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Thermo Fisher Scientific MicroSeq® ID Analysis Software Quick start guide

Thermo Fisher Scientific MicroSeq® ID Analysis Software Quick start guide

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