Technoclone Ceveron MFU 500 User manual

2

1

Introduction

The new filtration unit Ceveron

®

MFU 500 is specifically designed for

removal of particles larger than 0.2 µm and their collection from platelet poor

plasma and other cell poor body fluids under standardized conditions.

The Ceveron

®

MFU 500 is used with standard multi-well filter plates and

corresponding receiver plates for collecting the filtrates and the retained

particles. It allows rapid collection of filtrates and retained particles for a

large number of applications. It is specifically designed to remove and collect

micro particles from platelet poor plasma.

CONTENTS

1 ABOUT THIS MANUAL

2 SAFETY WARNINGS

3 PARTS AND THEIR FUNCTIONS

4 USING CEVERON

®

MFU 500

5 APPLICATION NOTES

6 REPLACING O-RING AND GASKET

7 CLEANING AND STORAGE

8 TROUBLE SHOOTING

9 TECHNICAL DATA

10 ORDERING INFORMATION

11 WARRANTY

3

4

1. ABOUT THIS MANUAL

This product is equipped with safety features. Nevertheless, read the safety

warnings carefully and use the product only as described in this manual to

avoid injury or damage. Keep this manual for future reference.

Symbols in this manual:

This danger symbol indicates potential injury or handling errors.



This information symbol indicates supplementary information

2. SAFETY WARNINGS

Not following the instructions as set forward in this manual;

makes void any warranty claims or claims for damages to

property and injuries to persons resulting from the use of

this equipment!

Ceveron



MFU 500 must not be used with any other type of power supply as

the one included!

Check safety features of the instrument regularly e.g. for damage to the

housing, vacuum line, etc.

Maintenance or repair must only be performed by a specialist! Do not open

the housing of the instrument, Opening the housing or modifications to or

attempts to repair the instrument will make void any warranty claims.

• The power supply is delivered with the appropriate plug for your country.

The voltage to be used in connection with Ceveron

®

MFU 500 is preset

to 12 V DC. The correct settings for your country are preset by

Technoclone as is the polarity of the voltage adapter. You must not

change the settings!

• The respective accident prevention regulations of the Employer and the

respective rules put forward by the respective Liability Insurance

Association for electrical systems and equipment must be observed.

• In training facilities the handling of power supplies must be supervised

by trained personal.

• Do not touch the power supply with wet hands and avoid water spillage

of the power supply.

• Capacitors in the instrument may still be charged even when the

instrument has been disconnected from the power supply.

• Never plug in the power supply immediately after it has been brought in

from the cold. Formation of condensing water can destroy the unit. Allow

the instrument to reach room temperature before plugging it in.

• The power supply output connecting cable must not be extended.

• The Power Supply can become hot during operation. Ensure the device

has sufficient ventilation. Ventilation slots must not be covered!

• The Power Supply must not be plugged in when the unit shows visible

sings of damage or the unit does no longer function.

5

6

g

3. PARTS AND THEIR FUNCTIONS

a

Filter Plate Sample collection

b

Manifold Collar

Holds multiwell filter plates tightly to the receiver

plates

Contains gasket and O-Ring (not visible, in the manifold collar)

> see 6. Replacing O-ring and gasket

c

Spacer Block Serves as support for the receiver plate.

d

Manifold Base

Is connected to the vacuum pump and holds the

spacer block

e

Start button Press to start the vacuum pump and the timer

f

Release Valve

button

Press to release the vacuum after the pump has

stopped

g

Case Contains vacuum pump-, and electronic parts

h

Manometer

(pressure gauge)

Gives the pressure during filtration in inch Hg or

kPa

i

Tubing, ¼” I.D. Connects Manifold to vacuum pressure pump

j

Vacuum Control

Valve

Has to remain closed during standard operation

k

Stop Cock Has to remain open during standard operation

l

Receiver plate Collects the filtrate

m

Power connector

plug

To connect the unit with the 12 V power supply

n

Power supply unit

To be plugged into the socket of a regular AC

electrical power line to supply Ceveron

®

MFU

500 with 12 V electrical power.

a

b

c

d

e

h

i

j

k

l

f

n

m

7

8

4. USING Ceveron

®

MFU 500

FIRST-TIME USE

Remove the foam pad which is placed between upper and lower part of the

manifold for protection during shipment.

The power supply is already set to the voltage of your country. The output

voltage is set to 12 Volts and must not be changed.

Checking the desired output voltage

• The In-Plug Power Supply as delivered together with Ceveron



MFU 500

is set to an output voltage of 12 V DC.

• Check if the DC output voltage is set to 12 Volt

• Should the output voltage accidentally be set to a different voltage, it has

to be changed before using it together with Ceveron



MFU 500.

• Before changing the output voltage remove the In-Plug Power Supply

from the AC socket

• Make sure that the In-Plug Power Supply is not connected to the

Ceveron



MFU 500.

• Use an appropriate screwdriver to set the output voltage at the yellow

switch to 12 Volt.

• Using higher voltages than 12 Volt will damage the pump inside the

Ceveron



MFU 500

• Using lower voltages than 12 Volt will result in poor function of the pump

and of Ceveron



MFU 500.

Change the output voltage only

in a powered-down condition!

Pay attention to the operating

voltage of 12 Volt! Likewise, pay

attention to the firm engagement of the

switch into the respective voltage

position!

2

1

OPERATING PROCEDURE

Plug the power supply n in a socket of the regular AC power line and the 12

V power cable into the socket in the grey case

m

.

Take off the upper part of the manifold

b

and place the receiver plate

l

on top of the white spacer block

c

.

Put the manifold collar b in place and put the filter plate

a

into the collar.

Pipette the samples into the filter plate.

Never use the unit

w

ithout

receiver plate! (The filtrate would be

lost and the manifold contaminated.)

Remember the orientation of the

plate, e.g. well A1 on the top left

position.

Make

sure the plate fits well on the

black gasket.

Make sure that the filter plate is in

the same orientation as the receiver

plate! (e.g. well A1 in the top left

position)!

Maximal sample volume for 96

-

well

filter plates: 350 µL

For filtration of plasma we recommend

to use 200 µL/well

Note: It is not necessary to fill all wells

of a plate.

9

10

Seal the filter plate tightly with a plate sealer foil (provided).

Stop Cock

k

has to be in the open position (in line with the tubing

i

).

Press the start button e.

Press the filter plate

a

Note: If a turbid plasma sample

cannot be filtered completely

within the preset time, repeating the filtration will not have an effect as

the filter is likely blocked! See 8. Trouble Shooting

Release the vacuum

After complete ventilation of the unit, first take off the filter plate

a

and then

the manifold collar b.

Finally remove the receiver plate

l

containing the filtrates

To obtain the best possible

vacuum, the whole plate has to

be sealed independent off the

number of filled wells. Make sure

the foil is tightly put on the surface

of the filter plate. We recommend

to use a fresh foil for every

filtration.

After the pump starts,

press the

filter plate

a

aa

a

shortly but tightly to

the manifold collar (b to ensure

tight fitting of the plate. The pump

stops automatically after ~ 3

minutes, the time required for

filtration of 200µL plasma.

To release the vacuum,

press the

ventilation valve button f

ff

f. The

pressure will fall to 0 at the

pressure gauge

h

.

The vacuum pump inside the unit

will generate a vacuum of ~ - 15

inHg (i.e. ~ - 45 kPa), The

pressure will be indicated on the

pressure gauge

h

.

Note: Filter plates and plate sealer

are intended for single use only !

Note: Receiver plates are intended

for single use only !

11

12

5. APPLICATION NOTES

Generation of micro particle free plasma samples and recovery of micro

particles from platelet poor plasma:

Ceveron

®

MFU 500 is designed to be used for filtration of fresh, clear,

platelet poor plasma samples.

For turbid plasma samples pre-filtration through another filter with larger

pores is required.

What is the efficiency for removal of micro particles ?

After filtration of plasma spiked with 10,000 MP/µl only 0.4% ± 0.004% of

MPs were found in the filtrate.

Recovery of micro particles from the filter plate

MPs can be recovered from the filter plate by rinsing each well with 200µL

of PBS or micro particle free plasma. Rinsing is performed by gentle

pipetting the sample for 3 to 5 times and then pipetting off the sample.

Recovery rates are in general > 85 %

Sample Material

Sample

Pre-filtration /

filter required

Notes

PPP NO

Collection of PPP under

standardized condition is required

PRP YES

Instead of pre-filtration prepare

PPP by standard centrifugation

procedure

Whole Blood YES

Prepare PPP by standard

centrifugation procedure

Lipaemic Plasma YES 5 µm filter

Lyophilized Plasma YES 5 µm filter

Some lyophilized plasma do not

require pre-filtration

6. REPLACING O-RING AND GASKET

An accessory kit containing an additional O-ring, gasket and Allen wrench is

included with each unit of Ceveron

®

MFU 500. O-ring and gasket should be

replaced when worn or vacuum leakage is observed.

For replacing the black gasket, the 12 screws have to be loosened by using

the Allen wrench included in the accessory kit. The O-ring can simply be

replaced manually.

7. CLEANING AND STORAGE

Basic preventive maintenance requires regularly cleaning of Ceveron

®

MFU

500.

Use a mild soap, standard laboratory detergent, a dilute solution of sodium

hypochlorite, or ethyl alcohol to clean all surfaces of the manifold. After

cleaning, rinse off any residual liquid with a soft cloth or paper towel

dampened in clean water, then wipe dry.

Should there be any liquid leakage into the manifold base or the vacuum

tubing, it can be removed by rinsing the base with distilled water while the

vacuum pump is running (the pump is water resistant). After rinsing with

water, air should be pumped through the unit at least for 2 runs to dry out

the system

Other than occasional cleaning, the Power Supply is maintenance-free. For

cleaning the case, use a clean, lint-free, antistatic and dry cleaning cloth.

Store in a controlled environment at a temperature between 15°C and 30°C.

13

14

8. TROUBLE SHOOTING

This section describes possible problems that may be encountered when

using Ceveron

®

MFU 500 and recommended procedures to solve these

problems.

SYMPTOM POSSIBLE CAUSE SOLUTION

Filter plate not covered

or sealed.

Use plate sealer

After starting the filtration - press the

filter plate a shortly but tightly to the

manifold collar to ensure tight fitting of

the plates

Poor contact of plates

a with gasket

Manifold collar b

Align plates

Vaccum control valve

open

j

Close the valve

Stop cock (k) in “off”

position

Put stop cock to “on” position

(in line with tubing)

NO OR WEAK

VACUUM

Gaskets

worn / compromised

Replace gaskets (See “6. Replacing

O-ring or Gaskets”)

NO FLOW

Plasma sample is not

platelet poor or

contains particles

(denatured protein,

fibrin, cells) that cause

plugging of the filter

Pre-filter the samples

LIQUID IN

VACUUM

TUBING i

Unit was used without

receiver plate or

leaking receiver plates

Rinse unit with distilled water followed

by pumping air through the unit

If problems continue after trying the suggested solutions, contact

sales@technoclone.com or your local distributor.

9. TECHNICAL DATA

This section lists specifications for Ceveron

®

MFU 500 including building

materials, storage conditions and dimensions.

Building materials:

Manifold Base: Anodized Aluminium

Gasket: EPDM Ethylene-propylene Polymer

O-ring: Silicone

Spacer: Delrin

Adaptor Collar: Stainless steel

Pump housing: Plastic material

Pump

Ceveron



MFU 500 is equipped with a diaphragm Liquid Pump.

Hydraulic data:

Free flow at U

n

400 ml/min

Max. pressure height at U

n

40 m H

2

0

Working range up to 15 m H

2

O

Suction height (dry) 6 m H

2

O

Electrical data:

Motor Permanent Magnet

Nominal Voltage 12 V DC

Nominal Speed 3000 rpm

Nominal Current 0,4 A

Starting Current 1,7 A

Power Consumption max 5 W

Insulation Class E

Protection Class IP20

EMC protection Basic EMC filter (2L)

Parts in contact with spilled liquids:

Pump head Polyarylamide (PA)

Diaphragm EPDM

Valves EPDM

15

16

Dimensions:

Length: 24 cm (9.4 in.)

Width: 18 cm (7.1 in.)

Height: 15.6 cm (6.1 in.)

Weight: 3.6 kg (7.93 lbs.)

Power supply

Ceveron



MFU500 is driven by an In-Plug power supply, type UPA-1200 /

UPA-2000.

Construction complies with EN 61558 / safety of Power supplies). In

addition it has been EMC-tested and fulfills the requirements of the

applicable European and national directives.

Conformance to these has been proven; the relevant statements and

documents are filed with the manufacturer.

Technical data power supply

Operating data : 100V ~ to 240 V ~ 50/60 Hz

Output voltage : 3-4,5-6-7,5-9-12 VDC switchable

Output current : UPA 1200: 1200 mA max.

UPA 2000: 2000 mA max.

Ripple : < approx. 100 mV

Dimensions (LxWxH) :UPA 1200: approx. 105 x 60 x 43 (mm)

UPA 2000: approx. 118 x 62 x 39 (mm)

(without AC plug adapter respectively)

10. ORDERING INFORMATION

REF 9821010 Ceveron



MFU 500 1pc

contains: 1 x Ceveron



MFU 500

1 x Adapter

1 x Replacement Kit (Gaskets)

1 x Filter plate 0.2 µm

1 x Receiver plate

2 x Plate Sealer Cover

1 x Warranty

1 x Manual

Additional Products:

REF 9821035 Filter plate 0.2 µm (96 wells) 1x

REF 9821040 Filter plates 0.2 µm (96 wells) 10x

REF 9821055 Receiver plate (96 wells) 1x

REF 9821060 Receiver plates (96 wells) 10x.

REF 9821033 Plate sealers for filter plates 10 pc.

REF 9821034 Plate sealers for filter plates 100 pc.

REF 9821013 Ceveron

®

MFU Replacement Accessory Kit (Gaskets)

Also see Technoclone products under

www.technoclone.com

or www.ceveron.com

17

11. WARRANTY

Technoclone gives a 12 months warranty from the date of purchase. Our

product was manufactured using the latest production techniques and has

passed quality control.

Damage due to improper use, worn parts and consumables are not covered

by the warranty. The warranty is void, if the unit has been subjected to

unauthorized manipulations.

If the unit has to be sent back to Technoclone for repair purposes, please

pay attention to proper packaging. Technoclone does not take responsibility

for any damages occurred during transport.

Note: Please return page 2 of the warranty card to your distributor or to

Technoclone GmbH. Credit or replacement of instrument / spare parts for

repair under warranty, can only be obtained when the distributor or

Technoclone GmbH has received pages 2 of the warranty card.

Notice:

The information in this document is subject to change without notice and

should not be construed as a commitment by Technoclone GmbH.

Technoclone GmbH assumes no responsibility for any errors that may

appear in this document. This manual is thought to be complete and

accurate at the time of publication. In no event shall Technoclone GmbH be

liable for incidental or consequential damages in connection with or arising

from the use of this manual.

Copyright

©

may be reproduced in any form or by any means whatsoever without prior written permission of

Technoclone GmbH.

3050510-003 Date of Issue: 12/2010

Technoclone GmbH

Brunner Str. 59/5, 1230 Vienna

AUSTRIA

Phone: + 43 1 86373-0

Fax: + 43 1 86373-44

Email: sales@technoclone.com

www.technoclone.com

Technoclone Ceveron MFU 500 User manual

Technoclone Ceveron MFU 500 User manual

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