Thermo Fisher Scientific TaqMan SCID SMA Plus Assay User guide
- Type
- User guide
TaqMan™ SCID/SMA Plus Assay
Catalog Numbers A48566, A48567, A48568, A48569
Pub. No. MAN0019382 Rev. A.0
Note: For safety and biohazard guidelines, see the “Safety” appendix in the TaqMan™ SCID/SMA Plus Assay User Guide
(Pub. No. MAN0019381). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves.
This Calibration Guide is intended to provide dye spectral calibration plate information for users of the TaqMan™ SCID/SMA Plus Assay.
For detailed instructions and ordering information for additional products, see the TaqMan™ SCID/SMA Plus Assay User Guide (Pub.
No. MAN0019381).
■Dye spectral calibration plates for the QuantStudio™ 6 / QuantStudio™ 7 Flex Real-Time PCR System ....................... 1
■Dye spectral calibration plates for the QuantStudio™ 12K Flex Real–Time PCR System ................................... 2
■Dye spectral calibration plates for the QuantStudio™ 5 Real‑Time PCR System ......................................... 3
■Dye spectral calibration plates for the QuantStudio™ Dx Real‑Time PCR Instrument (RUO mode) ........................... 3
Dye spectral calibration plates for the QuantStudio™ 6 / QuantStudio™ 7 Flex Real-Time PCR System
See your instrument user guide for recommended calibration schedules and detailed calibration instructions.
Table 1 QuantStudio™ Real‑Time PCR Software v1.6.1 or later
Plate[1] Cat. No.[2]
96-well 0.2 mL
QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, and SYBR™ dyes), 96‑Well 0.2‑mL A26331
QuantStudio™ 3/5 Spectral Calibration Plate 2, 96‑Well 0.2‑mL (ABY™, JUN™, and MUSTANG PURPLE™ dyes) A26332
96-well 0.1 mL
QuantStudio™ 3/5 Spectral Calibration Plate 1, (FAM™, VIC™, ROX™, and SYBR™ dyes), 96‑well, 0.1 mL A26336
QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96‑well Fast (0.1‑mL) Plate A26337
384-well
QuantStudio™ 5 Spectral Calibration Plate 1, (FAM™, VIC™, ROX™ TAMRA™, and SYBR™ dyes), 384-well A26334
QuantStudio™ 5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy®5 dyes), 384-well A26335
[1] Dye spectral calibration plates for the QuantStudio™ 3 and 5 Instruments are used for the calibration procedure with the QuantStudio™ Real‑Time PCR Software v1.6.1 or later.
[2] Catalog numbers that appear as links open the web pages for those products.
Table 2 QuantStudio™ Real‑Time PCR Software v1.3
Plate Cat. No.[1]
96-well 0.2 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24738
FAM™ Dye Spectral Calibration Plate, 96-well 4432327
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24737
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well 4461599
VIC™ Dye Spectral Calibration Plate, 96-well 4432334
CALIBRATION GUIDE
For Research Use Only. Not for use in diagnostic procedures.
Plate Cat. No.[1]
96-well 0.1 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24734
FAM™ Dye Spectral Calibration Plate, Fast 96-well 4432389
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24735
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well 4457328
VIC™ Dye Spectral Calibration Plate, Fast 96-well 4432396
384-well
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24736
FAM™ Dye Spectral Calibration Plate, 384-well 4432271
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24733
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well 4457334
VIC™ Dye Spectral Calibration Plate, 384-well 4432278
[1] Catalog numbers that appear as links open the web pages for those products.
Dye spectral calibration plates for the QuantStudio™ 12K Flex Real–Time PCR System
See your instrument user guide for recommended calibration schedules and detailed calibration instructions.
Plate Cat. No.[1]
96-well 0.2 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24738
FAM™ Dye Spectral Calibration Plate, 96-well 4432327
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24737
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well 4461599
VIC™ Dye Spectral Calibration Plate, 96-well 4432334
96-well 0.1 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24734
FAM™ Dye Spectral Calibration Plate, Fast 96-well 4432389
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24735
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well 4457328
VIC™ Dye Spectral Calibration Plate, Fast 96-well 4432396
384-well
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24736
FAM™ Dye Spectral Calibration Plate, 384-well 4432271
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24733
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well 4457334
VIC™ Dye Spectral Calibration Plate, 384-well 4432278
[1] Catalog numbers that appear as links open the web pages for those products.
2TaqMan™ SCID/SMA Plus Assay Calibration Guide
Dye spectral calibration plates for the QuantStudio™ 5 Real‑Time PCR System
See your instrument user guide for recommended calibration schedules and detailed calibration instructions.
Plate Cat. No.[1]
96-well 0.2 mL
QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, and SYBR™ dyes), 96‑Well 0.2‑mL A26331
QuantStudio™ 3/5 Spectral Calibration Plate 2, 96‑Well 0.2‑mL (ABY™, JUN™, and MUSTANG PURPLE™ dyes) A26332
96-well 0.1 mL
QuantStudio™ 3/5 Spectral Calibration Plate 1, (FAM™, VIC™, ROX™, and SYBR™ dyes), 96‑well, 0.1 mL A26336
QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96‑well Fast (0.1‑mL) Plate A26337
384-well
QuantStudio™ 5 Spectral Calibration Plate 1, (FAM™, VIC™, ROX™ TAMRA™, and SYBR™ dyes), 384-well A26334
QuantStudio™ 5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy®5 dyes), 384-well A26335
[1] Catalog numbers that appear as links open the web pages for those products.
Dye spectral calibration plates for the QuantStudio™ Dx Real‑Time PCR Instrument (RUO mode)
See your instrument user guide for recommended calibration schedules and detailed calibration instructions.
Plate Cat. No.[1]
96-well 0.2 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24738
FAM™ Dye Spectral Calibration Plate, 96-well 4432327
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well A24737
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well 4461599
VIC™ Dye Spectral Calibration Plate, 96-well 4432334
96-well 0.1 mL
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24734
FAM™ Dye Spectral Calibration Plate, Fast 96-well 4432389
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well A24735
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well 4457328
VIC™ Dye Spectral Calibration Plate, Fast 96-well 4432396
384-well
ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24736
FAM™ Dye Spectral Calibration Plate, 384-well 4432271
JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well A24733
MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well 4457334
VIC™ Dye Spectral Calibration Plate, 384-well 4432278
[1] Catalog numbers that appear as links open the web pages for those products.
TaqMan™ SCID/SMA Plus Assay Calibration Guide 3
Life Technologies Corporation | 6055 Sunol Blvd | Pleasanton, CA 94566 USA
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0019382
Revision Date Description
A.0 8 January 2021 New document.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all
applicable Limited Use Label Licenses.
©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a
registered trademark of Roche Molecular Systems, Inc., used under permission and license. Cy is a registered trademark of GE Healthcare.
thermofisher.com/support | thermofisher.com/askaquestion
thermofisher.com
8 January 2021
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Thermo Fisher Scientific TaqMan SCID SMA Plus Assay User guide
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- User guide
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