CEM Edge Quick start guide

EDGE Instructions Page 1 of 3

Quick Start Guide 2021-01

Quick Start Guide

Prepare a Solvent

Turn on the EDGE. The EDGE should boot up to a “One Touch Methods” and “User Methods” screen. Add the appropriate solvent(s) to

bottles, cap using the caps provide with the EDGE, and run the solvent line through the cap. Ensure the bottom of the line is in solvent.

Select the System Menu icon. Enter the “Settings” menu, and go to “Bottle Setup.” Select the solvent placed in the lines, save, and

allow the solvents to prime. For more information on how to do this, please refer to the EDGE Manual.

Prepare System for Running

Select the System Menu icon. Select the “Tools” menu, and enter “Utilities.” Select “System Wash.” Select the desired solvent, and

allow the system to wash. The waste solvent should be collected by the waste assembly. For more information on how to do this,

please refer to the EDGE Manual.

Prepare a Q-Cup

Assemble the Q-Cup with the appropriate Q-Disc by placing the Q-Disc of choice in the bottom of the Q-Cup and then replacing the

bottom back on the Q-Cup’s body. Weigh any sorbents directly into the Q-Cup. Then, weigh the sample into the Q-Cup on top of the

sorbent. Do not add sorbents and sample above the fill line indicated in the image. Then, place a Q-Screen on top of the sorbents and

sample. For more information on how and when to use a Q-Screen, please consult the Q-Screen Guide. Place the Q-Cup and a

collection vial compatible with the EDGE in the EDGE rack.

EDGE Instructions Page 2 of 3

Quick Start Guide 2021-01

Create a Method

Program a method by selecting “User Methods” from the main screen, and then select the Plus Sign icon at the top of the screen.

Within the “Create Method” screen, name the method accordingly, and select the Q-Disc of choice. Then, in the “Cycles” screen, select

the appropriate solvent, top volume, bottom volume, temperature, hold time, rinse solvent, and rinse volume. Then, select the

appropriate wash parameters in the “Wash” screen. It is recommended the wash solvent used is the same as the extraction solvent. If

more guidance is needed for the selection of these parameters, please consult the EDGE Application Notes, the EDGE manual, and

the Molecular Support team.

EDGE Instructions Page 3 of 3

Quick Start Guide 2021-01

Run a Method

Select a method for each Q-Cup prepared by selecting the method on the left and the corresponding sample and vial on the right of the

screen. Press the Play sign at the top of the screen. When the sample loads, if desired, edit the Sample ID by selecting “Sample ID,”

selecting the sample to give a Sample ID, naming appropriately, and then selecting “OK” to save. Each sample can be provided its own

Sample ID. For more information on Sample IDs, please consult the EDGE manual. When ready to run, press “Start.” Allow the

extraction to run. Remove the collection vial and Q-Cup when finished. The extract is filtered, cooled, and ready for post-extraction

workup. Examples of post-extraction workup are evaporation, dilution, further sample prep, etc. If more guidance is needed, please

consult the EDGE Application Notes or the Molecular Support team.