QUICK START
TM
AP 720S Semi-Automated Instrument
Note: The assumed starting point is the instrument state after
completion of a previous run and following routine maintenance and
shutdown. (The alternate state would be the interim between two
successive runs of the instrument). The wash buffer and deionized
water tubes will be on a clean surface following completion of the
drain function and the power will be off.
Prepare Serum Samples
1. Centrifuge all serum samples.
2. Transfer each serum sample into an appropriately labeled clean
plastic storage tube with lid.
Load Sample / Reagent Rack
3. Load the required number of empty Sample Cups into the
Sample / Reagent Rack. Start at position #1 and do not leave
any empty positions.
4. Transfer the correct volume of each prepared sample into the
sample cups:
600µl serum for a 36-allergen panel
490µl serum for a 20-allergen panel
Note: The red Sample Fill Line on the Sample / Reagent Rack can be
used as a guide to check that there is a fill volume of approximately
600µl in the Sample Cups.
5. Load the Antibody Reservoir (BLUE line) onto the Sample /
Reagent Rack.
6. Remove the Antibody Reagent bottle from the kit and gently
invert prior to use.
7. Fill the Antibody Reservoir with the required amount of reagent
as listed in the Instruction Manual.
8. Install the Sample / Reagent Rack on the instrument aligning
sample position #1 with the red indicator line and lowering the
rack so that the Center and Positioning Pin locate into the holes
at the bottom of the rack.
Load Pette Rack
9. Remove the required number of OPTIGEN Pettes from the kit
box and label with sample identification. (For a given run all
pettes must be of the same panel type, see Step 21 below).
Reseal the plastic bag and return the kit to the refrigerator.
10. Attach a Pette Tip to each Pette Body.
11. Place the Pette Rack on the Pette Stand. (Always use the Pette
Stand when loading or removing Pettes.)
12. Load the Pettes into the Pette Rack. Always start at position
#1 and do not leave any empty positions. Check that each Pette
Body is correctly secured in the Pette Rack. Ensure the number
of Pettes matches the number of samples.
13. Remove the Pette Rack from the Pette Stand.
14. Install the Pette Rack on the instrument by lowering it onto
the center guide bar with pette cartridge #1 positioned front
and center so that the rack is properly aligned with the
positioning pin.
15. Close the door of the instrument.
Load Wash Buffer and Deionized Water,
and Empty Waste
16. Prepare the Wash Buffer according to the instructions in the
OPTIGEN package insert.
17. Fill the Wash Buffer Bottle with the required amount of Wash
Buffer as listed in the Instruction Manual.
Secure the yellow cap on the Wash Buffer Bottle and ensure the
weighted end of the Wash Buffer tube is located at the bottom
of the bottle.
18. Ensure there is sufficient (1L) deionized water in the Deionized
Water Bottle and fill if needed. Secure the white cap on the
Deionized Water Bottle and ensure the weighted end of the
Deionized Water tube is located at the bottom of the bottle.
19. Empty the Waste Bottle by pressing the quick release connectors
to detach the tubes, unscrew the cap, and then empty the waste
liquid. Reconnect the Waste Bottle by screwing the cap back on
and tightening. Attach the quick release connectors to the cap,
matching the color-coded labels.
Doc. No. 0822 - ENG Rev. 00
www.hcdiagnostics.com
Start Processing
20. Turn on the AP 720S. After initializing, the [Main Menu] screen
will be displayed.
21. Select the panel type (Panel 20 or Panel 36). Press “Next” and
move to the [Check] screen.
22. Ensure there is sufficient Antibody Reagent in the Reagent
Reservoir, sufficient Wash Buffer in the Wash Buffer Bottle, and
sufficient Deionized Water in the Deionized Water Bottle, and
check that the Waste Bottle is empty.
23. Press “Start” to begin automated processing. (There are 8
processes in all to complete the Auto-Run).
Load Photoreagent Mixture
24. At the end of Process #7, the [Change Photo Rgt] will appear,
the AP 720S will pause and an alarm will sound. Press “Alarm Off”.
25. Prepare Photoreagent Mixture by mixing an equal amount of
Photoreagent AB and CD. Note: Allow Photoreagent AB and CD
to come to room temperature prior to mixing. Once mixed, the
Photoreagent should be used immediately for best results.
26. Fill the Photoreagent Reservoir (RED line) with the required
amount of Photoreagent Mixture as listed in the Instruction
Manual.
27. Open the door. Remove the Antibody Reservoir and load the
Photoreagent Reservoir in the Sample / Reagent Rack. Close
the door.
Continue Processing
28. Press “Continue” on the [Change Photo Rgt] screen.
29. Press “Yes” on the [Check] screen to start Process #8
(Photoreagent Aspiration).
30. As soon as the first pette is filled with Photoreagent Mixture,
set a timer for 10 minutes.
31. At the end of Process #8, an alarm will sound. Press “Alarm Off”
on [Process End] screen.
Unload and Read Pettes
32. Open the door, remove the Pette Rack from the instrument and
set on the Pette Stand.
33. Insert a black top plug into each Pette.
34. Unload the pettes one by one from the Pette Rack.
35. Remove the Pette Tip from each Pette.
36. Insert a white bottom plug into each Pette.
37. Load the Pettes in order into the Pette Cassette Tray.
38. When the timer reads 10 minutes load the tray in the CLA-1
Luminometer and read the test results.
Completion of Operation
39. Remove the Sample / Reagent Rack and discard all used sample
cups.
40. To run a second set of pettes immediately following the first set,
press “End” on the [Process End] screen to return to the [Main
Menu] screen.
41. Alternatively, to prepare for shutdown, press “Rinse” on the
[Process End] screen.
42. Unscrew the caps on the Wash Buffer and Deionized Water
bottles. Pull out the tube from the Wash Buffer Bottle and place
it in the Deionized Water Bottle along with the Deionized Water
tube. Press “Ok” in the [Rinse] screen.
43. On completion of the rinse remove the Wash Buffer and Deionized
Water tubes from the Deionized Water bottle. Place Wash Buffer
and Deionized Water tube on a clean surface. Press “Ok” on the
[Drain] screen.
44. After the drain function is finished the [Main Menu] screen
appears. Turn off instrument.
45. Rinse Reagent Reservoirs with deionized water.
Doc. No. 0822 - ENG Rev. 00
www.hcdiagnostics.com
QUICK START
TM
AP 720S Semi-Automated Instrument (continued)
[Main Menu]
[Panel Type]
[Check]
[Change Photo Rgt]
Elapsed Time 0:0:15
[Check]
Add Photo Rgt?
[Process End]
Elapsed Time 0:0:15
Antibody,
Wash Buffer,
Water.Waste
[Panel 36]
Assay Panel 36
Next
Back
Start
Alarm Off
Yes
Alarm Off
RinseEnd
No
Continue
[Rinse]
Please change tube
to Water Bottle.
[Drain]
Please pulll out tube
from Water Bottle.
Back Ok
End Ok
36 20
Maintenance
07/ 2/23
14:25:21
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