Hitachi AP 720S Quick Start

Brand: Hitachi

Model: AP 720S

Type: Quick Start

QUICK START

AP 720S Semi-Automated Instrument

Note: The assumed starting point is the instrument state after

completion of a previous run and following routine maintenance and

shutdown. (The alternate state would be the interim between two

successive runs of the instrument). The wash buffer and deionized

water tubes will be on a clean surface following completion of the

drain function and the power will be off.

Prepare Serum Samples

1. Centrifuge all serum samples.

2. Transfer each serum sample into an appropriately labeled clean

 plastic storage tube with lid.

Load Sample / Reagent Rack

3. Load the required number of empty Sample Cups into the

 Sample / Reagent Rack. Start at position #1 and do not leave

 any empty positions.

4. Transfer the correct volume of each prepared sample into the

 sample cups:

  600µl serum for a 36-allergen panel

  490µl serum for a 20-allergen panel

Note: The red Sample Fill Line on the Sample / Reagent Rack can be

used as a guide to check that there is a fill volume of approximately

600µl in the Sample Cups.

5. Load the Antibody Reservoir (BLUE line) onto the Sample /

 Reagent Rack.

6. Remove the Antibody Reagent bottle from the kit and gently

 invert prior to use.

7. Fill the Antibody Reservoir with the required amount of reagent

 as listed in the Instruction Manual.

8. Install the Sample / Reagent Rack on the instrument aligning

 sample position #1 with the red indicator line and lowering the

 rack so that the Center and Positioning Pin locate into the holes

 at the bottom of the rack.

Load Pette Rack

9. Remove the required number of OPTIGEN Pettes from the kit

 box and label with sample identification. (For a given run all

 pettes must be of the same panel type, see Step 21 below).

 Reseal the plastic bag and return the kit to the refrigerator.

10. Attach a Pette Tip to each Pette Body.

11. Place the Pette Rack on the Pette Stand. (Always use the Pette

 Stand when loading or removing Pettes.)

12. Load the Pettes into the Pette Rack. Always start at position

 #1 and do not leave any empty positions. Check that each Pette

 Body is correctly secured in the Pette Rack. Ensure the number

 of Pettes matches the number of samples.

13. Remove the Pette Rack from the Pette Stand.

14. Install the Pette Rack on the instrument by lowering it onto

 the center guide bar with pette cartridge #1 positioned front

 and center so that the rack is properly aligned with the

 positioning pin.

15. Close the door of the instrument.

Load Wash Buffer and Deionized Water,

and Empty Waste

16. Prepare the Wash Buffer according to the instructions in the

 OPTIGEN package insert.

17. Fill the Wash Buffer Bottle with the required amount of Wash

 Buffer as listed in the Instruction Manual.

 Secure the yellow cap on the Wash Buffer Bottle and ensure the

 weighted end of the Wash Buffer tube is located at the bottom

 of the bottle.

18. Ensure there is sufficient (1L) deionized water in the Deionized

 Water Bottle and fill if needed. Secure the white cap on the

 Deionized Water Bottle and ensure the weighted end of the

 Deionized Water tube is located at the bottom of the bottle.

19. Empty the Waste Bottle by pressing the quick release connectors

 to detach the tubes, unscrew the cap, and then empty the waste

 liquid. Reconnect the Waste Bottle by screwing the cap back on

 and tightening. Attach the quick release connectors to the cap,

 matching the color-coded labels.

Doc. No. 0822 - ENG Rev. 00

www.hcdiagnostics.com

Start Processing

20. Turn on the AP 720S. After initializing, the [Main Menu] screen

 will be displayed.

21. Select the panel type (Panel 20 or Panel 36). Press “Next” and

 move to the [Check] screen.

22. Ensure there is sufficient Antibody Reagent in the Reagent

 Reservoir, sufficient Wash Buffer in the Wash Buffer Bottle, and

 sufficient Deionized Water in the Deionized Water Bottle, and

 check that the Waste Bottle is empty.

23. Press “Start” to begin automated processing. (There are 8

 processes in all to complete the Auto-Run).

Load Photoreagent Mixture

24. At the end of Process #7, the [Change Photo Rgt] will appear,

 the AP 720S will pause and an alarm will sound. Press “Alarm Off”.

25. Prepare Photoreagent Mixture by mixing an equal amount of

 Photoreagent AB and CD. Note: Allow Photoreagent AB and CD

 to come to room temperature prior to mixing. Once mixed, the

 Photoreagent should be used immediately for best results.

26. Fill the Photoreagent Reservoir (RED line) with the required

 amount of Photoreagent Mixture as listed in the Instruction

 Manual.

27. Open the door. Remove the Antibody Reservoir and load the

 Photoreagent Reservoir in the Sample / Reagent Rack. Close

 the door.

Continue Processing

28. Press “Continue” on the [Change Photo Rgt] screen.

29. Press “Yes” on the [Check] screen to start Process #8

 (Photoreagent Aspiration).

30. As soon as the first pette is filled with Photoreagent Mixture,

 set a timer for 10 minutes.

31. At the end of Process #8, an alarm will sound. Press “Alarm Off”

 on [Process End] screen.

Unload and Read Pettes

32. Open the door, remove the Pette Rack from the instrument and

 set on the Pette Stand.

33. Insert a black top plug into each Pette.

34. Unload the pettes one by one from the Pette Rack.

35. Remove the Pette Tip from each Pette.

36. Insert a white bottom plug into each Pette.

37. Load the Pettes in order into the Pette Cassette Tray.

38. When the timer reads 10 minutes load the tray in the CLA-1

 Luminometer and read the test results.

Completion of Operation

39. Remove the Sample / Reagent Rack and discard all used sample

 cups.

40. To run a second set of pettes immediately following the first set,

 press “End” on the [Process End] screen to return to the [Main

 Menu] screen.

41. Alternatively, to prepare for shutdown, press “Rinse” on the

 [Process End] screen.

42. Unscrew the caps on the Wash Buffer and Deionized Water

 bottles. Pull out the tube from the Wash Buffer Bottle and place

 it in the Deionized Water Bottle along with the Deionized Water

 tube. Press “Ok” in the [Rinse] screen.

43. On completion of the rinse remove the Wash Buffer and Deionized

 Water tubes from the Deionized Water bottle. Place Wash Buffer

 and Deionized Water tube on a clean surface. Press “Ok” on the

 [Drain] screen.

44. After the drain function is finished the [Main Menu] screen

 appears. Turn off instrument.

45. Rinse Reagent Reservoirs with deionized water.

Doc. No. 0822 - ENG Rev. 00

www.hcdiagnostics.com

QUICK START

AP 720S Semi-Automated Instrument (continued)

[Main Menu]

[Panel Type]

[Check]

[Change Photo Rgt]

Elapsed Time 0:0:15

[Check]

Add Photo Rgt?

[Process End]

Elapsed Time 0:0:15

Antibody,

Wash Buffer,

Water.Waste

[Panel 36]

Assay Panel 36

Back

Start

Alarm Off

Yes

Alarm Off

RinseEnd

Continue

[Rinse]

Please change tube

to Water Bottle.

[Drain]

Please pulll out tube

from Water Bottle.

Back Ok

End Ok

36 20

Maintenance

07/ 2/23

14:25:21

Hitachi AP 720S Quick Start

Hitachi AP 720S Quick Start

Hitachi AP 720S Quick Start

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