GE EPS 3501 XL Operating Instructions Manual

GE Healthcare

Electrophoresis Power

Supply EPS 3501 XL

Operating Instructions

Original instructions

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 3

Table of Contents

1 Introduction .............................................................................. 5

1.1 Important user information .................................................................................5

1.2 Regulatory information ......................................................................................... 7

1.3 Instrument ................................................................................................................... 9

2 Safety instructions................................................................. 15

2.1 Safety precautions ............................................................................................... 15

2.2 Labels .......................................................................................................................... 18

2.3 Emergency procedures ...................................................................................... 20

2.4 Built-in safety features ....................................................................................... 20

2.5 Recycling information ......................................................................................... 21

3 Installation .............................................................................. 23

3.1 Site requirements .................................................................................................. 23

3.2 Unpacking ................................................................................................................. 23

3.3 Transport ...................................................................................................................23

3.4 Connections ............................................................................................................. 24

3.5 Spare parts and accessories ........................................................................... 25

4 Operation ................................................................................ 27

4.1 Operation overview .............................................................................................. 27

4.2 Starting the instrument ...................................................................................... 27

4.3 Setting up a run ..................................................................................................... 28

4.4 Preparations before start .................................................................................. 35

4.5 Performing a run ................................................................................................... 35

5 Maintenance ........................................................................... 41

5.1 General ....................................................................................................................... 41

6 Troubleshooting ..................................................................... 43

7 Reference information .......................................................... 45

7.1 Specifications .......................................................................................................... 45

7.2 Literature ................................................................................................................... 45

7.3 Ordering information ........................................................................................... 45

Appendix A Electrical and communication connections...... 47

Table of Contents

4Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 5

Introduction 1

Important user information 1.1

1 Introduction

Purpose of the Operating

Instructions

The Operating Instructions provide you with the instructions needed to handle

Electrophoresis Power Supply EPS 3501 XL in a safe way.

Prerequisites

In order to operate EPS 3501 XL safely and according to the intended purpose the

following prerequisites must be met:

• You should be acquainted with the use of general laboratory equipment and with

handling of biological materials.

• You should understand the concepts of electrophoresis.

• You must read the Safety Instructions in Chapter 2 of these Operating Instructions.

• The system should be installed according to the instructions in Chapter 3 of these

Operating Instructions.

In this chapter

This chapter contains important user information and a general description of EPS 3501

XL and its intended use.

1.1 Important user information

Read this before using EPS 3501

XL

All users must read the Safety Instructions in Chapter 2 of these Operating Instructions

before installing, using or maintaining the instrument.

Do not operate EPS 3501 XL in any other way than described in the user documentation.

If you do, you may be exposed to hazards that can lead to personal injury and you may

cause damage to the equipment.

1 Introduction

1.1 Important user information

6Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Intended use

EPS 3501 XL is a high quality, high precision power supply for electrophoresis

applications that require advanced programming and high voltage.

EPS 3501 XL is intended for research use only, and shall not be used in any clinical

procedures, or for diagnostic purposes.

Safety notices

These Operating Instructions contain WARNINGS, CAUTIONS and NOTICES concerning

the use of the product, with meanings as defined below.

WARNING

WARNING indicates a hazardous situation which, if not avoided, could

result in death or serious injury. It is important not to proceed until all

stated conditions are met and clearly understood.

CAUTION

CAUTION indicates a hazardous situation which, if not avoided, could

result in minor or moderate injury. It is important not to proceed until all

stated conditions are met and clearly understood.

NOTICE

NOTICE indicates instructions that must be followed to avoid damage to

the product or other equipment.

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 7

Introduction 1

Regulatory information 1.2

Notes and tips

Note: A Note is used to indicate information that is important for trouble-free and

optimal use of the product.

Tip: A tip contains useful information that can improve or optimize your procedures.

Typographical conventions

Software texts and commands are identified by bold italic text. A colon is used to

separate menu levels (e.g. File:Open refers to the Open option in the File menu).

1.2 Regulatory information

This section lists the directives and standards that are fulfilled by EPS 3501 XL.

Manufacturing information

CE Conformity

Requirement Content

Name and address of manufacturer GE Healthcare Bio-Sciences AB,

Björkgatan 30, SE 751 84 Uppsala

Sweden

Name and ID of notified body INTERTEK SEMKO AB, NB 0413

Place and date of declaration Uppsala, Sweden, May 2009

Identity of person authorized to sign DoC See EC Declaration of Conformity

Directive Title

2006/42/EC Machinery Directive (MD)

2006/95/EC Low Voltage Directive (LVD)

2004/108/EC ElectroMagnetic Compatibility (EMC) Directive

1 Introduction

1.2 Regulatory information

8Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

International standards

CE marking

The CE marking and the corresponding Declaration of Conformity is valid for the

instrument when it is:

• used as a stand-alone unit, or

• connected to other CE-marked instruments, or

• connected to other products recommended or described in the user

documentation, and

• used in the same state as it was delivered from GE Healthcare, except for

alterations described in the user documentation or explicitly authorized by

GE Healthcare.

Regulatory compliance of

connected equipment

Any equipment connected to EPS 3501 XL should meet the safety requirements of EN

61010-1/IEC61010-1 or relevant harmonized standards. Within the European Union,

connected equipment must be CE-marked.

Standard Description Notes

EN 61010-1,

IEC 61010-1,

CAN/CSA-C22.2

No. 61010-1

Safety requirements for electrical

equipment for measurement, control

and laboratory use

EN 61326-1 EMC emissions and immunity

requirements for electrical equipment

measurement, control and laboratory

use

Harmonized with

2004/108/EC

EN-ISO 12100-1,

12100-2

Safety of machinery – Basic concepts,

general principles and design

Harmonized with

2006/42/EC

EN-ISO 14121-1,

14121-2

Safety of machinery – Principles of risk

assessment

Harmonized with

2006/42/EC

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 9

Introduction 1

Instrument 1.3

1.3 Instrument

This chapter contains a general description of EPS 3501 XL.

General description

EPS 3501 XL is a high quality, high precision power supply for electrophoresis

applications that require advanced programming and high voltage.

EPS 3501 XL is primarily designed for techniques using programming in several phases

and/or voltage gradients:

• 2-D (Two dimensional) electrophoresis using Multiphor II Electrophoresis System

• IEF (Isoelectric focusing) using Immobiline™ or carrier ampholytes such as

CleanGel™ and ExcelGel™

• Flatbed SDS-PAGE (Polyacrylamide Gel Electrophoresis) or flatbed native PAGE

EPS 3501 XL is also suitable for:

•DNA sequencing

• Agarose electrophoresis

•Electroblotting

• DNA pulsed field electrophoresis

Nine programs each with up to nine phases can be saved. Limiting values for voltage,

current and power as well as voltage gradients can be programmed for precise control

of the electrophoresis. The EPS 3501 XL automatically switches over the controlling

parameter according to programmed limits and gradients and conductivity variations

in the system.

Two electrophoresis units can be connected to EPS 3501 XL and run with the same

programmed method at one time.

1 Introduction

1.3 Instrument

10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Instrument description

Figure 1-1. The main parts of the EPS 3501 XL instrument.

1

3

2

Part Description

1Display

2Keyboard

3Output sockets

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 11

Introduction 1

Instrument 1.3

Indicator and switch on the instrument unit

Front panel

The front panel consists of an alphanumeric display, a keyboard with 9 membrane keys,

a light emitting diode (LED) that lights when voltage is applied (HV on) and connectors

for two electrophoresis units.

Display

Indicator/Switch Color Description

HV ON

(indicator lamp)

Green The HV ON lamp indicates that the

high voltage power supply is on.

Power

(switch)

- Switches on/off power to the

instrument. Located on the rear

panel.

HV on

ST OP

pa

con

delete

MAINS

Disconnect before serv

I

0

1

4

23

5

86

7

Part Description Part Description

1Mode 5Breakpoint parameter

2Voltage 6Phase number

3Current 7Control mode (step)

4Power 8Program number

1 Introduction

1.3 Instrument

12 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Keyboard

Key Label Description

set enter Press set enter to enter a value or choice, confirm this if

valid, and move programming to the next field. Valid

values are voltage 35–3 500 V, current 1–400 mA, power

1–200 W, time 0:01–500 h, volthours 1–500 000 Vh,

milliampere-hours 1–25 000 mAh.

In the RUN mode, pressing set enter shows the

programmed parameters for the actual run. In addition,

set enter allows you to make changes in the program

during a run after first pressing pause continue.

After a run, when in END mode, pressing set enter puts

the instrument into SET, its programming mode.

-Press to change the parameter, value or other

choice in the field which is blinking. Numerical values are

changed in an accelerating manner when a key is held

down. Clicking a key changes the value in preset

increments. Parameters or units (e.g. Vh) and choices (e.g.

Yes/No) are changed with one key push. The keys can also

be used to switch between time and volthours in RUN,

PAUSE and END. The values scroll i.e. they automatically

change from maximum to minimum value or vice versa.

run Press run to start the run and put the program into RUN

mode. The current values for voltage, current and power

are shown on the display. The elapsed time, volthours or

milliamperehours are also displayed. Switch between

these last three parameters with 

pause

continue

Press pause continue to put the instrument in PAUSE

mode and switch off the voltage. The display shows the

status of the run at the time the key was pressed. pause

continue only operates in RUN mode. Time, integrated

voltage and integrated current are retained.

In the PAUSE MODE mode, set enter can be used to make

changes in the program. Return to RUN mode by pressing

pause continue or by pressing run.

STOP Press STOP to stop the run and put the instrument in END

mode. The voltage is switched off and the end parameters

are displayed. Switch between time, integrated voltage

and integrated current by pressing . A run cannot be

continued after pressing STOP. Press run to run the same

method again or press set enter to choose another

program, program a new method or make changes in an

existing method.

ent er

set

ru n

pause

continue

ST OP

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 13

Introduction 1

Instrument 1.3

Output sockets

There are two sets of output sockets to allow two electrophoresis units to be connected

and run at the same time.

insert

delete

Press insert delete to insert or delete a phase in a

program. This function is activated in SET mode. Note that

a program must be completed by answering Yes (Y) to the

question “Last Phase?” in SET mode or by pressing exit

before you can use the insert delete key.

more Press more to place program in MORE mode. This gives

access to some special functions. These include:

COPY: Copying a program.

CLEAR: Clearing a program.

SETUP: Disabling the start current check.

MORE mode cannot be activated in RUN or PAUSE mode.

Leave MORE by pressing exit.

exit Press exit to stop the execution of an operation, such as

the entry of a value. Only values/units that have already

been confirmed by set enter are retained when exit is

pressed. Note that if a phase contains zeros when pressing

exit, that phase will be deleted.

Press exit to return the instrument to the mode that was

left or to the start position in SET.

Key Label Description

Inser t

delet e

mo re

exit

+

-

Output socket Label Color Voltage

Positive +Red 0 and +1750 V

Negative -Black or blue 0 and –1750 V

1 Introduction

1.3 Instrument

14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 15

Safety instructions 2

Safety precautions 2.1

2 Safety instructions

This chapter describes safety compliance, safety labels, general safety precautions,

emergency procedures, power failure and recycling of EPS 3501 XL.

2.1 Safety precautions

Introduction

The EPS 3501 XL is powered by mains voltage and handles liquids that may be

hazardous. Before installing, operating or maintaining the equipment, you must be

aware of the hazards described in this manual. Follow the instructions provided to avoid

personal injury or damage to the equipment.

The safety precautions in this section are grouped into the following categories:

• General precautions

• Personal protection

• Installing and moving the instrument

•System operation

•Maintenance

General precautions

WARNING

Do not operate the EPS 3501 XL in any other way than described in the

EPS 3501 XL User Manual.

WARNING

Operation and user maintenance of EPS 3501 XL should be performed

by properly trained personnel only.

WARNING

Do not use any accessories not supplied or recommended by

GE Healthcare.

2 Safety instructions

2.1 Safety precautions

16 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

Personal protection

Installing and moving the

instrument

WARNING

Do not use EPS 3501 XL if it is not working properly, nor if it have

suffered any damage, for example:

• damage to the power cord or its plug

• damage caused by dropping the equipment

• damage caused by splashing liquid onto items not supplied or

recommended by GE Healthcare.

WARNING

Operation and user maintenance of EPS 3501 XL should be performed

by properly trained personnel only.

WARNING

EPS 3501 XL is a high voltage instrument that can cause fatal electrical

shock if the safety features are disabled. The safety lid must be securely

closed before starting a protocol.

WARNING

The instrument covers must not be opened by the user. It contains

electrical circuits which can give a lethal electric shock. Service and

planned maintenance should be performed by personnel authorized by

GE Healthcare.

WARNING

Always use appropriate personal protective equipment during

operation and maintenance of EPS 3501 XL.

WARNING

Supply voltage. Make sure that the supply voltage at the wall outlet

corresponds to the marking on the instrument, before connecting the

power cord.

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 17

Safety instructions 2

Safety precautions 2.1

System operation

Maintenance

WARNING

Protective ground. The EPS 3501 XL must always be connected to a

grounded power outlet.

WARNING

Power cord. Only use power cords delivered or approved by

GE Healthcare.

WARNING

Access to power switch and power cord. Do not block the rear panel of

the instrument. The Power switch must always be easy to access. The

power cord must always be easy to disconnect.

WARNING

Hazardous biological agents during run. When using hazardous

biological agents, flush the entire system tubing with bacteriostatic

solution followed by a neutral buffer and finally distilled water, before

service and maintenance.

WARNING

Avoid spilling liquids on the body of the instrument. If large volumes of

liquid have penetrated the casing of the instrument and come into

contact with the electrical components, immediately switch off the

instrument and contact an authorized service technician.

WARNING

Do not remove the main cover. There are no user serviceable

components inside, and you can be exposed to high voltage.

2 Safety instructions

2.2 Labels

18 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

2.2 Labels

This section describes the safety labels and labels concerning hazardous substances

that are attached to EPS 3501 XL.

Labels on the instrument

Rear panel labels

The illustration below shows an example of the identification label that is attached to

EPS 3501 XL.

Front panel labels

Symbols used in safety labels

28-9269-30 ETTAN IPGphor 3

Made in Sweden

28952336aa

GE Healthcare Bio-Sciences AB

751 84 Uppsala Sweden

Serial No: 1234567

N12406

Code No: 11003364

Mfg Year: 2009

Voltage: 100-240 V

50-60 Hz

Frequency:

Power max: 230 VA

Fuse: 2x T2.5AL 250 V

XX-XXXX-XX EPS 3501 XL

Made in Sweden

28952336aa

GE Healthcare Bio-Sciences AB

751 84 Uppsala Sweden

Serial No XXXXXXX

N12406

Code No: XXXXXXXX

Mfg Year: 2009

Voltage:

Frequency:

Power max:

Fuse:

Label Meaning

Warning! Read the user documentation before using the system. Do

not open any covers or replace parts unless specifically stated in the

user documentation.

Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA 19

Safety instructions 2

Labels 2.2

Labels concerning hazardous

substances

Indicates that hazardous voltages are generated inside the

instrument.

The system complies with the requirements for electromagnetic

compliance (EMC) in Australia and New Zealand.

The system complies with applicable European directives.

Label Meaning

This symbol indicates that the waste of electrical and electronic

equipment must not be disposed as unsorted municipal waste and

must be collected separately. Please contact an authorized

representative of the manufacturer for information concerning the

decommissioning of equipment.

This symbol indicates that the product contains hazardous materials

in excess of the limits established by the Chinese standard SJ/

T11363-2006 Requirements for Concentration Limits for Certain

Hazardous Substances in Electronics.

2 Safety instructions

2.3 Emergency procedures

20 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA

2.3 Emergency procedures

This section describes how to do an emergency shutdown of EPS 3501 XL. The section

also describes the result in the event of power failure.

Emergency procedures

In an emergency situation, do as follows to stop the run:

Power failure

The result of a power failure depends on which unit that is affected.

2.4 Built-in safety features

The power supply has the following built-in safety functions:

Step Action

1Switch off power to the instrument by pressing the Power switch to the 0

position. If required, disconnect the mains power cord. The run is

interrupted immediately.

Power failure to... will result in...

EPS 3501 XL The run is interrupted immediately, in an undefined state

Function Description

Functional earth

leakage

Should the power supply be connected to an electrophoresis

unit that has a leakage path to earth, the EPS 3501 XL will

detect this fault and turn off the high voltage.

Start current check The power supply checks that the resistance is not higher

than a specified limit at a low safety voltage (<40V). If this

resistance is too high, the high voltage is turned off.

Too high resistance can be caused by for example:

- an electrophoresis unit incorrectly connected

- use of buffer with exremely low conductivity

This function can be disabled to perform certain applications,

see Electrophoresis Power Supply EPS 3501 XL User Manual.

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GE EPS 3501 XL Operating Instructions Manual

GE EPS 3501 XL Operating Instructions Manual

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