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Thermo Fisher Scientific Preparation of DNA Samples User guide

  • I have reviewed the user guide for DNA Loading Dye, specifically the MassRuler R0621, Orange R0631, and TriTrack R1161 variants. It details a straightforward protocol for preparing DNA samples for conventional electrophoresis, involving mixing specific ratios of dye with the sample. I'm ready to answer your questions about this process and the loading dyes.
  • What is the recommended ratio of loading dye to DNA sample?
    What is the next step after mixing the loading dye and DNA sample?
Protocol
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Preparation of DNA Samples for
Conventional DNA Electrophoresis
6x DNA Loading Dye, 6x Thermo Scientic MassRuler DNA Loading Dye (Cat #R0621),
6x Orange DNA Loading Dye (Cat #R0631), 6x Thermo Scientic TriTrack DNA Loading Dye
(Cat #R1161) are all used according to below protocol:
1. Add 1 volume of 6x DNA loading dye to 5 volumes of DNA sample.
2. Mix well, spin down and load.
This protocol is for Preparation of DNA Samples for Conventional DNA Electrophoresis.
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