ThermoFisher Scientific Q33230 User guide

Brand: ThermoFisher Scientific

Model: Q33230

Type: User guide

ThermoFisher Scientific Q33230 Qubit 1X dsDNA HS Assay Kit empowers life science researchers with accurate and rapid DNA quantification for various applications. It quantifies DNA samples from 200 pg/µL to 1000 ng/µL in just two minutes, enabling quick and efficient downstream analysis.

QUICK REFERENCEQUICK REFERENCE Qubit™ Assays

For Research Use Only. Not for use in diagnostic procedures.

Pub. No. MAN 0017210

Rev. C.0

QUICK REFERENCE

Qubit™ Assays

1. Set up two assay tubes for the standards (three for

the protein or RNA IQ assay) and one assay tube for

each sample.

2. Prepare the Qubit™ working solution by diluting the

Qubit™ reagent 1:200 in Qubit™ buer. Prepare 200 μL of

working solution for each standard and sample.

†

3. Prepare the assay tubes* according to the table below.

Standard assay tubes User sample assay tubes

Working solution† (from step 2) 190 μL 180 –199 μL

Standard (from kit) 10 μL —

User sample —1–20 μL

Total Volume in each assay tube 200 μL 200 μL

† Qubit 1X dsDNA assays (Cat. Nos. Q33230, Q33231, Q33265, Q33266) are supplied with a ready-to-use working solution, and do not require preparation.

* Use only thin-wall, clear 0.5 mL PCR tubes. Acceptable tubes include Qubit™ assay tubes (set of 500, Cat. No. Q32856).

General Qubit Assay Protocol

4. Vortex all tubes for 2–3 seconds.

5. Incubate the tubes for 2 minutes at room temperature

(15 minutes for the Qubit™ protein assay).

6. Insert the tubes in the Qubit™ Fluorometer and take

readings. For detailed instructions, refer to the Qubit™

Fluorometer manual.

Standards from kit

User samples

Qubit™

buffer

Qubit™ reagent

Ensure all reagents are

at room temperature

Qubit™

working solution

1 × n μL*

199 × n μL*

* where n = number of standards plus number of samples

190 μL

180-199 μL

Final Qubit™ volume tube is 200 μL

Final Qubit™ tube volume is 200 μL

1-20 μL 1-20 μL 1-20 μL

10 μL 10 μL

1. Set up two assay tubes for the standards, one assay tube

for a buer blank and one assay tube for each sample.

2. Prepare assay tubes according to the table below. Adding

solutions in the following order to the assay tubes: (1)

Standards/Sample/Sample Buer, (2) Protein BR Assay

Buer, (3) Protein BR Assay Reagent.

3. Vortex for 5-7 seconds immediately after addition of

Protein BR Assay Reagent.

4. Incubate tubes for 10 minutes at room temperature.

5. Insert the tubes in the Qubit™ Fluorometer and take

readings. For detailed instructions, refer to the Qubit™

Fluorometer manual.

the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 12 March 2021

Find out more at thermoﬁsher.com/qubit

Limited Product Warranty

Life Technologies Corporation and/or its afﬁliate(s) warrant their products as set forth in the Life

Technologies’ General Terms and Conditions of Sale found on Life Technologies’ website at www.

thermoﬁsher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please

contact Life Technologies at www.thermoﬁsher.com/support.

The information in this document is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS

AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR

CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING

YOUR USE OF IT.

Important Licensing Information: These products may be covered by one or more Limited Use Label

Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use

Label Licenses.

Manufacturer: Multiple Life Technologies Corporation manufacturing sites are responsible for

manufacturing the products associated with the workﬂow covered in this guide.

For Qubit Protein BR Assay only

Standard assay tubes Buffer blank User sample assay tubes

Standard (from kit) 20 µL - -

User sample buffer -10 or 20 µL -

User sample - - 10 or 20 µL

Protein BR Assay Buffer 150 µL 150 or 160 µL 150 or 160 µL

Protein BR Assay Reagent 30 µL 30 µL 30 µL

Total volume in each assay tube 200 µL 200 µL 200 µL

STD 1 STD 2 Sample Blank

Protein BR

Assay Buffer

Step 1: Prepare

assay tubes

Protein BR

Reagent

Add 10 or 20 µL Mix & vortex for 5 sec

Final volume is 200 µL

Add 30 µL

Add 150 or 160 µL

Step 2: Add Protein BR

Assay Buffer

Step 3: Add Protein

BR Reagent

Step 4: Incubate for

10 min at RT

Step 5:

Read samples

ThermoFisher Scientific Q33230 User guide

ThermoFisher Scientific Q33230 User guide

ThermoFisher Scientific Q33230 User guide

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