Thermo Fisher Scientific VeriSpray PaperSpray Ion Source User guide

Thermo Scientific

VeriSpray PaperSpray

Ion Source

User Guide

98200-98015 Revision A April 2019

FreeStyle, TSQ Fortis, and VeriSpray are trademarks; and Thermo Scientific, TSQ Altis, TSQ Endura, TSQ

Quantiva, TSQ Quantis, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United

States. Fisher Scientific is a registered trademark of Fisher Scientific Co. in the United States.

PaperSpray is a trademark of the Purdue Research Foundation.

The following are registered trademarks in the United States and other countries: Kimwipes is a registered

trademark of Kimberly-Clark Corporation. Liquinox is a registered trademark of Alconox, Inc. Microsoft and

Windows are registered trademarks of Microsoft Corporation.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Rev A, April 2019

Software version: TSQ Series II 3.2 instrument control software and later

For Research Use Only. Not for use in diagnostic procedures.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable North American and European regulations. Your system meets the applicable requirements in the

electromagnetic compatibility (EMC) and product safety standards described in this section.

Unauthorized changes that you make to your system will void regulatory compliance and may defeat the built-in

protections for your instrument. Some examples of unauthorized changes include using replacement parts or adding

components, options, or peripherals that Thermo Fisher Scientific has not qualified and authorized. Unauthorized

changes can also result in bodily injury and/or damage to your system and laboratory.

Ensure continued compliance with regulatory standards:

• Follow all installation instructions provided in the documentation that comes with your system.

• Order replacement parts (as specified in the instrument manual) and additional components, options, and

peripherals directly from Thermo Fisher Scientific or an authorized representative.

Low Voltage Directive 2014/35/EU

This device complies with Low Voltage Directive 2014/35/EU safety standards EN 61010-1:2010, UL/CSA

61010-1:2012; EN/UL/CSA 61010-2-081:2015. .

EMC Directive 2014/30/EU and other EMC test standards

This device was tested by TÜV Rheinland and complies with the following EMC standards:

EN 61326-1 and subordinate EMC standards 47 CFR 15, Subpart B, Class A: 2015

Immunity: Emission:

• IEC/EN 61326-1:2013

• EN 55011:2009/A1:2010

• CISPR11:2009+A1:2010

• AS/NZS CISPR32; FCC Title 47, Part 15 Subpart B, Class A:2017

• ICES-003 Issue 6

• VCCI CISPR 32

• IEC/EN 61326-1:2013

• EN 61000-4-2:2009

• EN 61000-4-3:2006/A2:2010

• EN 61000-4-4:2004+A1:2010

• EN 61000-4-5:2006

• EN 61000-4-6:2009

• EN 61000-4-8:2010

• EN 61000-4-11:2004

RoHS II Directive 2011/65/EU

FCC Compliance Statement

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: This instrument must be used in the manner specified by Thermo Fisher

Scientific to ensure protections provided by the instrument are not impaired. Deviations from specified instructions on

the proper use of the instrument include changes to the system and part replacement. Accordingly, order replacement

parts from Thermo Fisher Scientific or one of its authorized representatives.

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

WEEE Directive

2012/19/EU

Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the

European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway.

If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business

(B2B) Recycling Program, send an email request to weee.recycle@thermofisher.com with the following information:

• WEEE product class

• Name of the manufacturer or distributor (where you purchased the product)

• Number of product pieces, and the estimated total weight and volume

• Pick-up address and contact person (include contact information)

• Appropriate pick-up time

• Declaration of decontamination, stating that all hazardous fluids or material have been removed from the product

For additional information about the Restriction on Hazardous Substances (RoHS) Directive for the European Union,

search for RoHS on the Thermo Fisher Scientific European language websites.

IMPORTANT This recycling program is not for biological hazard products or for products that have been medically

contaminated. You must treat these types of products as biohazard waste and dispose of them in accordance with

your local regulations.

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide vii

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Accessing Documentation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Compatible Mass Spectrometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv

Special Notices, Symbols, and Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv

Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi

Safety Labels on the VeriSpray Ion Source . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

VeriSpray System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

VeriSpray Ion Source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Solvent Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Plate Transport Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Ion Source Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Barcode Scanner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Safety Interlock System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

VeriSpray Sample Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Manual Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Automated Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Magazine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Sample Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Alignment Plate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Plate Barcodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Ion Source LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Automated Plate Loader LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

PaperSpray Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Preparing the System and the Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Running the Samples. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Chapter 2 Laboratory Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Power Outlet Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Workbench Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Contents

viii VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

Workbench Clearance Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Recommended Layout for an MS with a VeriSpray System . . . . . . . . . . . . . . . . 20

Chapter 3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Installing the VeriSpray Ion Source on an MS. . . . . . . . . . . . . . . . . . . . . . . . . . 23

Preparing the MS for the VeriSpray Ion source . . . . . . . . . . . . . . . . . . . . . . . 24

Removing the Cover from an Uninstalled Ion Source . . . . . . . . . . . . . . . . . . 24

Mounting the Ion Source onto the MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Connecting the Ion Source to the Data System . . . . . . . . . . . . . . . . . . . . . . . 28

Connecting the Ion Source to Line Power . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Completing the VeriSpray System Installation. . . . . . . . . . . . . . . . . . . . . . . . 29

Installing the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Adjusting the Distance Between the Paper Tip and the MS Inlet . . . . . . . . . . . 30

Installing the Manual Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Installing the Automated Plate Loader. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Installing the Plate Transport Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Removing the Cover from an Installed Ion Source . . . . . . . . . . . . . . . . . . . . . . 37

Uninstalling the Automated Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Uninstalling the Manual Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Returning the Plate Slider to the Home Position. . . . . . . . . . . . . . . . . . . . . . . . 38

Running the Automated Post-Installation Calibration Procedures. . . . . . . . . . . 39

Uninstalling the VeriSpray System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Chapter 4 Automated System Calibration Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

Calibrating the Solvent Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Calibrating the Plate Transport Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Calibrating the Automated Plate Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Chapter 5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Turning the Ion Source Power On or Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Calibrating the Mass Accuracy of the MS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Optimizing the MS Parameters for Your Analytes . . . . . . . . . . . . . . . . . . . . . . . 46

Compatible Rewetting and Spray Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Filling the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Preparing the Sample Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Loading the Sample Plates into the Automated Plate Loader . . . . . . . . . . . . . . . 50

Mounting a Sample Plate onto the Manual Plate Loader. . . . . . . . . . . . . . . . . . 51

Priming the Ion Source Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Reading the Barcodes on the Plates in the Automated Plate Loader. . . . . . . . . . 53

Running a Paper Spray Experiment from the Tune Application . . . . . . . . . . . . 54

Setting Up the Solvent Dispense Program in the Tune Application. . . . . . . . . . 57

Setting Up the Paper Spray Parameters in an Instrument Method. . . . . . . . . . . 58

Contents

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide ix

Working with Xcalibur Sequences. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Specifying the Sample Positions in an Xcalibur Sequence . . . . . . . . . . . . . . . 60

Nomenclature for the Sample Positions in an Xcalibur Sequence . . . . . . . . . 62

Starting a Sequence Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Chapter 6 Data Review. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65

Reading the Plate Barcode and Strip Location from Qual Browser . . . . . . . . . . 65

Reading the Plate Barcode and Strip Location from FreeStyle . . . . . . . . . . . . . . 66

Chapter 7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Tools and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

Opening the Ion Source Door. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

Removing and Reinstalling the Ion Transfer Tube . . . . . . . . . . . . . . . . . . . . . . 72

Cooling the API Source Interface for Maintenance . . . . . . . . . . . . . . . . . . . . 72

Removing the Ion Transfer Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Reinstalling the Ion Transfer Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Replacing the High-Voltage Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

Maintaining the External Surfaces of the VeriSpray System. . . . . . . . . . . . . . . . 79

Maintaining the Sample Plates Before Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Disposing of Used Sample Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

Cleaning the Ion Source Sensor Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Chapter 8 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81

Top-Level VeriSpray Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Installation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Replaceable System Components and Custom Tools. . . . . . . . . . . . . . . . . . . . . 83

Consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85

Contents

xVeriSpray PaperSpray Ion Source User Guide Thermo Scientific

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide xi

F

Figure 1. Ion source mounting panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii

Figure 2. View of the API source interface when the ion source door is opened . . . . . . . . . 2

Figure 3. View of the HV probe and solvent dispense nozzles . . . . . . . . . . . . . . . . . . . . . . . 3

Figure 4. Plate transport platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Figure 5. Plate transport module with its cover removed XXX . . . . . . . . . . . . . . . . . . . . . . 5

Figure 6. Power switch on the lower right side of the ion source . . . . . . . . . . . . . . . . . . . . . 6

Figure 7. Manual plate loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Figure 8. VeriSpray Plate Loader (front and back) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Figure 9. Exploded and assembled views of the magazine . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Figure 10. 24-count sample plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Figure 11. Alignment plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Figure 12. Top view (footprint) and recommended placement of the workbench . . . . . . . . 19

Figure 13. Workbenches for a TSQ Altis MS with the VeriSpray system . . . . . . . . . . . . . . 20

Figure 14. Data System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Figure 15. Back view of the front cover showing the alignment rails and magnets . . . . . . . . 24

Figure 16. Removing the plate transport cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Figure 17. Locking levers in the Open position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Figure 18. Back of the ion source with a view of the mounting holes . . . . . . . . . . . . . . . . . 26

Figure 19. Mounting the ion source onto the mounting rails . . . . . . . . . . . . . . . . . . . . . . . 27

Figure 20. Locking levers in the Locked position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Figure 21. Drain pipe over the drain port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Figure 22. Alignment plate with its pin extended into the target window . . . . . . . . . . . . . 31

Figure 23. Z-axis adjustment screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Figure 24. Aligning the positioning pins to the mounting holes. . . . . . . . . . . . . . . . . . . . . 33

Figure 25. Plate loader bridge shown in angled position above the ion source transport rail 34

Figure 26. Tightening the bolt that secures the automated plate loader to the ion source. . 35

Figure 27. Cable connections to the plate transport module . . . . . . . . . . . . . . . . . . . . . . . 35

Figure 28. View of the mounted ion source without its cover . . . . . . . . . . . . . . . . . . . . . . 36

Figure 29. Diagnostics pane of the Tune application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Figure 30. Calibrate Solvent Module selected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Figure 31. Calibrate Plate Transport selected. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Figure 32. Calibrate Plate Loader Module selected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Figure 33. Closeup of the target area markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Figure 34. Spotting a paper strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Figure 35. Sample plate installed in slot 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Figure 36. Installing a sample plate on the manual plate loader . . . . . . . . . . . . . . . . . . . . . 51

Figure 37. Xcalibur Status page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Figures

xii VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

Figure 38. Ion Source pane of the Tune application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Figure 39. Default settings for the Continuous Acquisition mode . . . . . . . . . . . . . . . . . . . . 55

Figure 40. Data acquisition pane set up to use an instrument method . . . . . . . . . . . . . . . . 56

Figure 41. Global Parameters page of the Method Editor . . . . . . . . . . . . . . . . . . . . . . . . . . 59

Figure 42. New Sequence Template . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Figure 43. Xcalibur sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Figure 44. Run Sequence dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Figure 45. Raw data file opened in Qual Browser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Figure 46. Heading Editor dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Figure 47. File Header report in the FreeStyle application . . . . . . . . . . . . . . . . . . . . . . . . . 66

Figure 48. Sample Information report with the strip position . . . . . . . . . . . . . . . . . . . . . . 67

Figure 49. Allen wrench inserted through the side hole in the removal tool . . . . . . . . . . . . 73

Figure 50. Releasing the ion transfer tube from the spray cone . . . . . . . . . . . . . . . . . . . . . . 74

Figure 51. View of the tube’s nose cone and the removal tool’s slotted end . . . . . . . . . . . . . 75

Figure 52. Pulling the ion transfer tube out of a TSQ Altis MS . . . . . . . . . . . . . . . . . . . . . 75

Figure 53. Pulling the ion transfer tube out of a TSQ Fortis MS . . . . . . . . . . . . . . . . . . . . 76

Figure 54. Installing the ion transfer tube in a TSQ Altis MS . . . . . . . . . . . . . . . . . . . . . . 77

Figure 55. Installing the ion transfer tube in a TSQ Fortis MS . . . . . . . . . . . . . . . . . . . . . 77

Figure 56. View of the high-voltage probe from the bottom of the ion source door . . . . . . 78

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide xiii

P

Preface

The VeriSpray PaperSpray Ion Source User Guide describes how to install, operate, and

maintain the Thermo Scientific™ VeriSpray™ Ion Source and sample handling system.

To suggest changes to the documentation or to the Help

Complete a brief survey about this document by clicking the button below.

Thank you in advance for your help.

Accessing Documentation

Thermo Scientific mass spectrometers (MSs) include complete documentation. For system

requirements refer to the release notes on the software DVD.

To view the product manuals

• (Windows 7) From the Microsoft™ Windows™ taskbar, choose Start > All Programs >

Thermo Instruments > MS model x.x, and then open the applicable PDF file.

• (Windows 10) From the Windows taskbar, choose Start > All Apps> Thermo

Instruments > MS model x.x, and then open the applicable PDF file.

• From the Method Editor view for the MS, choose MS model > Manuals.

Contents

•Accessing Documentation

•Compatible Mass Spectrometers

•Special Notices, Symbols, and Cautions

•Safety Precautions

•Safety Labels on the VeriSpray Ion Source

•Contacting Us

Preface

xiv VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

To view user documentation from the Thermo Fisher Scientific website

1. Go to thermofisher.com.

2. Point to Services & Support and click Manuals on the left.

3. In the Refine Your Search box, search by the product name.

4. From the results list, click the title to open the document in your web browser, save it, or

print it.

To return to the document list, click the browser Back button.

Compatible Mass Spectrometers

Install the VeriSpray ion source on any of the Thermo Scientific TSQ Series II mass

spectrometers.

If you have instrument compatibility questions, contact Thermo Fisher Scientific Customer

Service.

Special Notices, Symbols, and Cautions

Make sure you understand the special notices, symbols, and caution labels in this guide. Most

of the special notices and cautions appear in boxes; those pertaining to safety also have

corresponding symbols. Some symbols are also marked on the API source itself and can

appear in color or in black and white. For complete definitions, see Table 1.

Table 1. Notices, symbols, labels, and their meanings (Sheet 1 of 2)

Notice, symbol, or label Meaning

IMPORTANT Highlights information necessary to prevent damage to

software, loss of data, or invalid test results; or might contain

information that is critical for optimal performance of the

product.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Caution: Highlights hazards to humans, property, or the

environment. Each CAUTION notice is accompanied by an

appropriate CAUTION symbol.

Chemical hazard: Highlights a chemical hazard to humans.

Preface

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide xv

Hot surface: Highlights a hot surface hazard to humans.

Risk of electric shock: Highlights an electric shock hazard to

humans.

Risk of eye injury: Highlights an eye hazard to humans.

Trip obstacle: Highlights trip hazards to humans.

Pinch point: Highlights hazards from moving parts that are

accessible to the user. Do not place your hands in the way of

these moving parts.

Table 1. Notices, symbols, labels, and their meanings (Sheet 2 of 2)

Notice, symbol, or label Meaning

Preface

xvi VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

Safety Precautions

Observe the following safety precautions when you operate or perform service on the

VeriSpray system.

CAUTION Do not perform any servicing other than that contained in this manual. To

avoid personal injury or damage to the instrument, do not perform any servicing other

than that contained in this manual or related manuals unless you are qualified to do so.

CAUTION Take care when moving the ion source or the automated plate loader.

Although the ion source and the automated plate loader are light enough for one person

alone to lift, they are unwieldy. Use a cart when moving the hardware from one bench to

another.

CAUTION Do not touch heated zones. Allow the API interface to cool to room

temperature before you service it. The API interface and the mass spectrometer’s ion

transfer tube can be very hot and can cause severe burns if touched.

CAUTION This ion source uses voltages that can cause electric shock and personal injury.

While operating the ion source, keep the covers on. Before removing the ion source from

the MS, turn the ion source off and unplug its power supply module.

CAUTION Observe safe laboratory practices and procedures when handling chemicals.

Only work with volatile chemicals under a fume or exhaust hood. Wear gloves and other

protective equipment, as appropriate, when handling toxic, carcinogenic, mutagenic,

corrosive, or irritant chemicals. Use approved containers and proper procedures to dispose

of solvents.

CAUTION Risk of eye damage. Eye injury can occur from splattered chemicals, airborne

particles, or sharp objects. Wear safety glasses when handling samples or chemicals.

CAUTION Risk of pinched skin. Take care when working with the automated plate

loader.

Never place your hand into the automated plate loader while the elevator magazine is in

motion. Wait until its motion ceases before you load or remove a plate.

CAUTION Risk of tripping. Be aware of cords, hoses, or other objects located on the floor.

Preface

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide xvii

Safety Labels on the VeriSpray Ion Source

Figure 1 shows the two safety labels that appear on the ion source’s mounting panel.

Figure 1. Ion source mounting panel

The generic Caution symbol is for the exposed wires.

The hot surface symbol warns you that the MS inlet can reach temperatures high enough to

cause burns.

CAUTION Do not touch the exposed wires. Take care to avoid disturbing the exposed

wires above the MS inlet when mounting the VeriSpray Ion Source. If you accidentally

loosen or disconnect any of the exposed wires, only a Thermo Fisher Scientific service

engineer may repair the damage.

CAUTION Do not touch heated zones. Allow the API interface to cool to room

temperature before you service it. The API interface and the mass spectrometer’s ion

transfer tube can be very hot and can cause severe burns if touched.

Preface

xviii VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

Contacting Us

Contact Email Telephone QR Code

U.S. Technical Support us.techsupport.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

U.S. Customer Service

and Sales

us.customer-support.analyze@thermofisher.com (U.S.) 1 (800) 532-4752

Global Support To find global contact information or customize your request

1. Go to thermofisher.com.

2. Click Contact Us, select the country, and then select the type of support

you need.

3. At the prompt, type the product name.

4. Use the phone number or complete the online form.

To find product support, knowledge bases, and resources

Go to thermofisher.com/us/en/home/technical-resources.

To find product information

Go to thermofisher.com/us/en/home/brands/thermo-scientific.

Note To provide feedback for this document, go to surveymonkey.com/s/PQM6P62 or send an email message to

Technical Publications (techpubs-lcms@thermofisher.com).

Thermo Scientific VeriSpray PaperSpray Ion Source User Guide 1

1

Introduction

This chapter provides a basic overview of the VeriSpray system and PaperSpray™ technology.

VeriSpray System

The VeriSpray system is a batch sampling system that uses PaperSpray technology to generate

analyte ions for the Thermo Fisher Scientific TSQ II series of mass spectrometers. The system

consists of a 24-count sample plate, a manual plate loader or an optional robotic sample

handler, and the ion source.

VeriSpray Ion Source

The ion source consists of two components: a plate transport module with a robotic arm that

moves the sample plate into the ion source and aligns the samples with the API source

interface and a solvent module that provides the sample rewetting solvent, the spray solvent,

and the ionizing voltage.

For information about the ion source components, see these topics:

•Solvent Module

•Plate Transport Module

•Ion Source Power Switch

Contents

•VeriSpray System

•VeriSpray Ion Source

•VeriSpray Sample Handling System

•Status LEDs

•PaperSpray Workflow

•Specifications

1 Introduction

VeriSpray Ion Source

2VeriSpray PaperSpray Ion Source User Guide Thermo Scientific

•Barcode Scanner

•Safety Interlock System

Solvent Module

The solvent module at the top of the ion source holds two 100 mL solvent bottles (Figure 2).

The bottle on the left holds the sample rewetting solvent, and the bottle on the right holds the

spray solvent. An integrated tubing assembly connects each solvent bottle to the associated

pump inlet inside the solvent module.

The ion source door opens to expose the API source interface. The barcode scanner is visible

from the lower left of the door and a safety interlock is visible in the upper right of the door’s

back panel.

Figure 2. View of the API source interface when the ion source door is opened

From the bottom of the door, you can see the high-voltage (HV) probe that provides the spray

voltage during a PaperSpray experiment and the two solvent dispense nozzles.

No. Description No. Description

1 Status LED (three LEDs) 2 API source interface (MS inlet)

3 Solvent bottles and tubing assemblies 4 Solvent module door handle

5 Barcode scanner window 6 Safety interlock

1 2

3

4

5

6

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Thermo Fisher Scientific VeriSpray PaperSpray Ion Source User guide

Thermo Fisher Scientific VeriSpray PaperSpray Ion Source User guide

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