Thermo Fisher Scientific EVOS Onstage Incubator User guide

Type
User guide
EVOS Onstage Incubator (OSI2) Oxygen Sensor Replacement
Kit
Catalog Number AMEP4977
Doc. Part No. 710511 Pub.No. MAN0029524 Rev. A.0
Note: For safety and biohazard guidelines, see the "Safety" appendix in the EVOS Onstage Incubator (OSI-2) User Guide (Pub. No.
MAN0027987). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing,
and gloves.
Kit contents
Oxygen sensor
3.0 mm Hex key
2.5 mm Hex key
• Oring
• Instructions
Replace the oxygen sensor
1. Power o the EVOS Onstage Incubator (OSI-2), then unplug the power cord, the USB port cable, and the 6-pin sensor data cable
from the control unit of the incubator.
2. Turn o the regulators on the gas tanks, then detach the gas lines from the control unit.
3. Remove the water reservoir.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1 2
1Water reservoir
2Control unit
4. Turn the control unit upside-down, then use the 3.0mm hex key to remove the 6 screws that fasten the bottom cover of the control
unit.
Note: When removing the bottom cover, place the control unit on a soft surface and hold the control unit carefully. The plastic skin of
the control unit is textured and it is susceptible to visible marring if placed on a rough or dirty surface.
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
2
1 1 1
1 1 1
1Screws
2Control unit
5. Remove the bottom cover of the control unit, then use the 2.5mm hex key to remove the two screws that attach the O2 sensor
bracket to the control unit.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
1
2
1Screws
2O2 sensor bracket
6. Disconnect the plug on the oxygen sensor.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
12 3
1
1Oxygen sensor plug
2Oxygen sensor
3O2 sensor bracket
2EVOS Onstage Incubator (OSI2) Oxygen Sensor Replacement Kit
7. Unscrew the old oxygen sensor by twisting it counter-clockwise, then remove it from the control unit.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
8. Unpack the new oxygen sensor from the kit and verify that the Oring is installed on the new oxygen sensor. If not, install the Oring
as shown in the image.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
1Oring
9. Screw in the new oxygen sensor into the O2 sensor bracket, then reconnect the plug to the oxygen sensor.
10. Perform steps 1–5 in reverse order to reassemble and reinstall the control unit.
11. Calibrate the new oxygen sensor.
Calibrate the oxygen sensor (EVOS M7000 Imaging System)
1. Plug the power cord into the power input jack on the control unit and the wall outlet.
2. Connect the control unit and the computer with the USB cable, then plug the 6-pin sensor data cable from the environmental
chamber into the appropriate input jack on the control unit.
3. Re-attach the gas lines (Air and Nitrogen or CO2) via the PTC (push-to-click) connectors to the control unit, then turn on the valves
on the gas tanks and regulators.
4. First power on the EVOS Onstage Incubator, then power on the EVOS M7000 Imaging System.
EVOS Onstage Incubator (OSI2) Oxygen Sensor Replacement Kit 3
5. Start the EVOS M7000 Software, navigate to the Settings tab
1
in the upper right of the screen, then open the Incubator menu
2
.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
1Settings tab
2Incubator menu
6. Open the Oxygen sensor4Calibrate controls
1
from inside the Incubator window.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
3
4
1Calibrate controls
2Oxygen %
3Purge gas selection
4Begin Calibration button
7. Set the Oxygen%
2
to the oxygen content of the air used by the system.
Note: The default value for Oxygen% is 20.95% for normal compressed air.
8. Select the purge gas to use: Nitrogen or CO2
3
.
4EVOS Onstage Incubator (OSI2) Oxygen Sensor Replacement Kit
9. Press Begin Calibration
4
to start the calibration routine.
The calibration process takes approximately three minutes to complete. When complete, the system displays the “Calibration was
successful." message next to the Begin Calibration button.
If there was an error during calibration, the system displays the "Calibration error, please check if gas is connected." message.
10. If there is a calibration error, verify that the gas is connected, the gas valves and regulators are on, and the correct gas was selected
as the purge gas, then repeat the calibration routine.
Note: If the calibration error persists, contact Technical Support.
11. Press Done when finished.
Calibrate the oxygen sensor (EVOS M5000 Imaging System)
1. Plug the power cord into the power input jack on the control unit and the wall outlet.
2. Connect the control unit and the computer with the USB cable, then plug the 6-pin sensor data cable from the environmental
chamber into the appropriate input jack on the control unit.
3. Re-attach the gas lines (Air and Nitrogen or CO2) via the PTC (push-to-click) connectors to the control unit, then turn on the valves
on the gas tanks and regulators.
4. First power on the EVOS Onstage Incubator, then power on the EVOS M5000 Imaging System.
5. Start the EVOS M5000 Software, click the Settings button
1
in the upper right of the screen, then click to open the Incubator
menu
2
.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
3
1Settings button
2Incubator menu
3Oxygen Sensor Calibration button
EVOS Onstage Incubator (OSI2) Oxygen Sensor Replacement Kit 5
6. Click Oxygen Sensor Calibration
3
in the Incubator menu to open the Incubator Oxygen Sensor Calibration window.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
3
1O2 %
2Purge gas selection
3Begin Calibration button
7. Set the O2%
1
to the oxygen content of the air used by the system.
Note: The default value for O2% is 20.95% for normal compressed air.
8. Select the purge gas to use: Nitrogen or CO2
2
.
9. Click Begin Calibration
3
to start the calibration routine.
The calibration process takes approximately three minutes to complete. When complete, the system displays the “Calibration was
successful." message under the Begin Calibration button.
If there was an error during calibration, the system displays the "Calibration error, please check if gas is connected." message.
10. If theres is a calibration error, verify that the gas is connected, the gas valves and regulators are on, and the correct gas was
selected as the purge gas, then repeat the calibration routine.
Note: If the calibration error persists, contact Technical Support.
11. When finished, click Close to close the calibration window, then click Done to go back to the main instrument screen.
Limited product warranty
Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the Life Technologies' General Terms and
Conditions of Sale at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please
contact Life Technologies at www.thermofisher.com/support.
Life Technologies Corporation | 22025 20th Ave SE Ste. 100 | Bothell, Washington 98021 USA
Revision history:Pub.No.MAN0029524 A.0
Revision Date Description
A.0 21 June 2023 Initial release.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all
applicable Limited Use Label Licenses.
©2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
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21 June 2023
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Thermo Fisher Scientific EVOS Onstage Incubator User guide

Type
User guide

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