Brooks CryoPod Carrier User manual

CryoPod™ Carrier

Operator Manual

Part Number: 257437 Rev. A

Brooks Automation, Inc.

Information provided within this document is subject to change without notice, and although believed

to be accurate, Brooks Automation, Inc. assumes no responsibility for any errors, omissions, or

inaccuracies.

ABF™, AcuLigner™, Advan Tag™, Align™, AquaTran™, AutoTeach™, ATR™, AXM™, Basic

Blue™, BioStore™, BiSymmetrik™, CenterSmart™, Cool Solutions™, Crate to Operate™,

CrossingConnect™, Cryotiger™, DARTS™, Enerta™, e-RMA™, e-Spares™, e-Volution™,

Falcon™, FastRegen™, FIXLOAD™, FrogLeg™, InLigner™, InCooler™, Interface™, Isoport™,

Jet™, Jet Engine™, LowProfile™, M2 Nano™, Mini-Ion™, PASIV™, PowerPak™,

PerformanceBlue™, Plate Auditor™, PowerPak™, PowerTools™, QuadraFly™, Radius™,

Radient™, Radient Express™, RapidThaw™, Reliance™, Reliance ATR™, RetroEase™,

SCARA™, SmartPM™, SPOTLevel™, Sprint™, Synetics™, The New Pathway to Productivity™,

Time Optimized Trajectory™, Time Optimal Trajectory™, Time Optimized Path™, TopCooler™,

TopLigner™, Tube Auditor™, Ultimate Blue™, VAC-407™, VacuTran™, VersaPort™,

WaferEngine™, and the Brooks logo are trademarks of Brooks Automation, Inc.

AcuTran®, AquaTrap®, Asyst®, the Cool Solutions logo, Crossing Automation®, Cryodyne®, Cryo-

Torr®, Fusion®, GOLDLink®, Guardian®, GUTS®, Helix®, Leapfrog®,MagnaTran®, MapTrak®,

Marathon®, Marathon 2®, Marathon Express®, MiniConvectron®, On-Board®, Polycold®, Razor®,

REMP®, Spartan®, TrueBlue®, TurboPlus®, Vision®, Xpeel®, XTape®, Zaris®, and the Brooks Life

Science Systems logo are registered U.S. trademarks of Brooks Automation, Inc.

All other trademarks are properties of their respective owners.

Proprietary Information of Brooks Automation, Inc. and is provided for the use of Brooks customers

only and cannot be used for distribution, reproduction, or sale without the express written permission

of Brooks Automation, Inc. This information may be incorporated into the user’s documentation;

however any changes made by the user to this information are the responsibility of the user.

ii

CryoPod™ Carrier Operator Manual CryoPod Carrier

Brooks Automation, Inc. Part Number: 257437 Rev. A

Corporate Headquarters

15 Elizabeth Drive

Chelmsford, MA 01824 U.S.A.

For Technical Support:

Location GUTS® Contact Number

North America

+1-800-FOR-GUTS (1-800-367-4887)

+1-978-262-2900

Europe +49-1804-CALL-GUTS (+49-1804-2255-4887)

Japan +81-45-477-5980

China +86-21-5131-7066

Taiwan +886-3-5525225

Korea +82-31-288-2500

Singapore +65-6464-1481

Visit us online: www.brooks.com

Contact Technical Publications directly: Technical.Publications@brooks.com

This technology is subject to United States export Administration Regulations and authorized to the

destination only; diversion contrary to U.S. law is prohibited.

Original manual printed in English. Printed in the U.S.A.

Accelerating Innovation

iii

Brooks Automation CryoPod™ Carrier Operator Manual

Part Number: 257437 Rev. A Brooks Automation, Inc.

Brooks Automation,

Inc.

15 Elizabeth Drive

Chelmsford, MA

01824-2400

Tel: +1 978-262-2400

Fax: +1 978-262-2500

www.brooks.com

Brooks Locations Worldwide:

Brooks Automation Inc.

46702 Bayside Parkway

Fremont,CA 94538

Tel: +1-510-661-5000

Fax: +1-510-661-5166

Brooks Life Science Systems

Northbank, Irlam

Manchester M44 5AY

United Kingdom

Tel: +44 (0) 161 777 2000

Fax: +44 (0) 161 777 2002

Brooks Life Science Systems

1003 E Trent Street, Suite 110

Spokane, WA 99202

Tel: +1 234-567-8910

Fax: +1 234-567-8910

Brooks Automation, Inc.

Polycold Systems

3800 Lakeville Highway

Petaluma, CA 94954

Tel: +1 707-769-7000

Fax: +1 707-769-1380

Brooks Automation, Inc.

MicroTool Products

824 South Tejon Street

Colorado Springs, CO 80903

Tel: +1 719-471-9888

Fax: +1 719-471-9977

Brooks Automation, Inc.

9601 Dessau Road, Suite 301

Austin, TX 78754

Tel: +1 512-912-2800

Fax: +1 512-912-2888

Brooks Automation Korea, Inc.

400-2 Gomae-Dong,

Giheung-Gu, Yongin-City

Gyeonggi-Do, 446-901

Korea

Tel : +82-31-288-2500

Fax: +82-31-287-2111

Brooks Automation France

SAS Les Jardins de Maupertuis

7 Chemin de la Dhuy

Batiment Le Juparana

Meylan, France 38240

Tel: +33 (0)4.76.18.92.00

Fax: +33 (0)4.76.18.91.98

Brooks Japan K.K.

HEADQUARTERS

Nisso Bldg. No 16, 10F

3-8-8 ShinYokohama, Kohoku-ku

Yokohama, Kanagawa 222-0033

Tel: +81-45-477-5570

Fax: +81-45-477-5571

Brooks Automation, Inc.

AIM Servicios Administrativos S de RL de

CV

Carretera Huinalá km 2.8

Parque Industrial Las Américas

66640 Apodaca, NL Mexico

Tel: +52 81 8863-6363

Brooks Automation Ltd.

TAIWAN HEADQUARTERS

5F-5, No.32, Tai-Yuen Street

Chu-Pei City

Hsinchu County 302, Taiwan, R.O.C.

Tel: +886-3-552 5258

Fax (G&A): +886-3-552 5255

Fax (Sales): +886-3-552 5200

Brooks Automation (Germany)

GmbH

Ernst-Ruska-Ring 11

07745 Jena, Germany

Tel: +49 3641 4821 100

Fax: +49 3641 4821 4100

Brooks Automation (Israel) Ltd.

Mevo Yerach 5

Kiryat-Gat 82000

Israel

Tel: +972 8672 2988

Fax: +972 8672 2966

Brooks Automation

(Germany) GmbH

RFID Division

Gartenstrasse 19

95490 Mistelgau, Germany

Tel: +49-9279-991 550

Fax: +49-9279-991 501

Brooks Automation

(Germany) GmbH

Karl-Marx-Strasse 23

D-01109 Dresden, Germany

Tel: +49 351 885 930

Fax: +49 351 885 9322

Brooks Technology

(Shanghai) Limited

2nd Floor, No. 72, 887

Zuchongzhi Road

Zhangjiang Hi-Tech Park

Pudong, Shanghai

China 201203

Tel: +86-21-5131-7070

Fax: +86-21-5131-7068

Brooks Life Science Systems

Weststrasse 12

CH-3672 Oberdiessbach

Switzerland

Tel: +41 (0) 31 770 70 70

Fax: +41 (0) 31 770 72 66

Brooks Automation

(Singapore) Pte Ltd

1200 Depot Road

#07-01 to #07-06

Singapore 109675

Tel: +65-6836-3168

Fax: +65-6836-3177

iv

CryoPod™ Carrier Operator Manual CryoPod Carrier

Brooks Automation, Inc. Part Number: 257437 Rev. A

Revision History

Part Number: 257437

CryoPod™ Carrier Operators Manual

Revision ECO Number Date Explanation of Changes

Revision A 90760 1/19/15 Initial completion of the manual.

v

Brooks Automation CryoPod™ Carrier Operator Manual

Part Number: 257437 Rev. A Revision History

Table of Contents

CryoPod™ Carrier Operator Manual i

Part Number: 257437 Rev. A i

Brooks Automation, Inc. ii

Revision History v

Table of Contents vi

1. Safety 1

Authorized Personnel Only 1

Explanation of Hazards and Alerts 2

Safety Text 2

Safety Icons 2

Signal Words and Color 2

Alert Example 3

General Safety Considerations 3

Safety and Identification Labels 5

Illustration of Label Location 6

Fire Hazards 7

Explosion Hazards 7

Thermal Hazards 7

Chemical Hazards 8

Ergonomic Hazards 8

Tip Hazard 8

Lift Hazard 8

Environmental Information 9

Noise Emission 9

Vibration 9

Decommissioning and Disposal 9

Regulatory Compliance and Declaration of Conformity (DOC) 10

2. Overview 11

Using this Manual 12

Specifications 13

System Button Selections 14

LCD System Display 14

LCD Lighting 14

LCD Control Buttons 15

LCD Indicators and Symbols 16

Concepts and Terminology 18

3. Installation 20

Unpacking 20

Package Components 20

CryoPod Carrier Registration and Software Download 20

CryoPod Carrier Assembly 21

SET Mode 22

Default SET Mode settings 22

vi

CryoPod™ Carrier Operator Manual CryoPod Carrier

Table of Contents Part Number: 257437 Rev. A

Manually Setting the CryoPod Carrier Displays 22

4. Operation 23

Materials needed (not provided by Brooks Automation) 23

Manual Filling of LN₂ 24

Inserting and Transporting Biospecimens 26

CryoPod Carrier Data Log Software 27

Software Installation and Set Up 27

Downloading Temperature Log Data 29

System Alarms 30

Alarm 1: TWARNING 30

Alarm 2: TCRITICAL 31

5. Preventative Maintenance 32

Cleaning the CryoPod Carrier 32

Removing the Replacing the LN₂Absorbent Pads 32

6. Troubleshooting 35

Functionality Check 35

Service indicator 35

Service and Error Codes 35

Contact Information for Brooks Life Science Systems 37

vii

Brooks Automation CryoPod™ Carrier Operator Manual

Part Number: 257437 Rev. A Table of Contents

1. Safety CryoPod Carrier

Authorized Personnel Only Part Number: 257437 Rev. A

1. Safety

Failure to review the Safety chapter and follow the safety warnings

can result in death or serious injury.

l All personnel involved with the operation or maintenance

of this product must read and understand the information

in this safety chapter.

l Follow all applicable safety codes of the facility as well as

national an international safety codes.

l Know the facility safety procedures, safety equipment, and

contact information.

l Read and understand each procedure before performing

it.

It is the responsibility of each person working on this product to know the

applicable regulatory safety codes as well as the facility safety procedures,

safety equipment, and contact information.

Authorized Personnel Only

The CryoPod™ Carrier is intended for use by trained and experienced laboratory personnel. It's

operators are advised to comply with applicable organizational standard operating procedures,

industry standard guidelines, and local, regional, national, and international laws and regulations.

1

Brooks Automation 1. Safety

Part Number: 257437 Rev. A Explanation of Hazards and Alerts

Explanation of Hazards and Alerts

This manual and this product use industry standard hazard alerts to notify the operator of personal or

equipment safety hazards. Hazard alerts contain Safety Text, Safety Icons, Signal Words, and Color.

Safety Text

Hazard alert text follows a standard, fixed-order, three-part format.

l Identify the hazard,

l State the consequences if the hazard is not avoided,

l State how to avoid the hazard.

Safety Icons

l Hazard alerts contain Safety Icons that graphically identify the hazard.

l The safety icons in this manual conform to ISO 3864 and ANSI Z535 standards.

Signal Words and Color

Signal Words inform of the level of hazard.

Danger indicates a hazardous situation which, if not

avoided, will result in death or serious injury.

Danger signal word is white on a red background with an

iconic exclamation point inside a yellow triangle with black

border.

Warning indicates a hazardous situation which, if not

avoided, could result in death or serious injury.

Warning signal word is black on an orange background

with an iconic exclamation point inside a yellow triangle

with black border.

Caution indicates a hazardous situation or unsafe practice

which, if not avoided, may result inminor or moderate

personal injury.

Caution signal word is black on a yellow background with

an iconic exclamation point inside a yellow triangle with

black border.

Indicates a situation or unsafe practice which, if not

avoided, may result in equipment damage.

Notice signal word is white on blue background with no

icon.

2

1. Safety CryoPod Carrier

General Safety Considerations Part Number: 257437 Rev. A

Alert Example

The following is an example of a WARNING hazard alert.

Figure 1-1: Components of a Safety Alert

General Safety Considerations

Unauthorized Service

Personal injury or damage to equipment may result if this product

is operated or serviced by unauthorized personnel.

l Only qualified personnel are allowed to transport, assemble,

operate, or maintain the Product.

l Properly qualified personnel are those who have received

training and have the proper qualifications for their jobs.

3

Brooks Automation 1. Safety

Part Number: 257437 Rev. A General Safety Considerations

Personal Protective Equipment

This product contains objects that may cause personal harm.

l Wear protective eye wear when setting up or testing the

system.

l Use appropriate thermal protective gloves when working

with components of the system.

Inappropriate Use

Use of this product in a manner or for purposes other than for what

it is intended may cause equipment damage or personal injury.

l Only use the product for its intended application.

l Do not modify this product beyond its original design.

l Always operate this product with the covers in place.

Damaged Components

The use of this product when components appear to be damaged

may cause equipment malfunction or personal injury.

l Do not use this product if components appear to be

damaged.

l Place the product in a location where it will not get

damaged.

4

1. Safety CryoPod Carrier

Safety and Identification Labels Part Number: 257437 Rev. A

Seismic Restraint

The use of this product in an earthquake prone environment may

cause equipment damage or personal injury.

The operator is responsible for determining whether the product is

used in an earthquake prone environment and installing the

appropriate seismic restraint in accordance with local regulations.

Do not use the CryoPod Carrier as a cryogenic shipper.

Do not place near or immerse in water or other liquids.

Do not remove the electronic module from the system.

Safety and Identification Labels

Safety labels are placed on the CryoPod Carrier to identify hazards. Identification labels provide

information about the product. Safety Labels provide instructions on how to avoid the hazard.

5

Brooks Automation 1. Safety

Part Number: 257437 Rev. A Safety and Identification Labels

Do not change or remove the safety or equipment identification labels.

To replace a lost or damaged label call Brooks Life Science Systems Technical Support.

Hazard Label Type or Name: Extreme Cold

p/n: 255864

Qty: 1

Location: Top of CryoPod Carrier

Possible injuries: Tissue Damage, Cold

Burns, and Frostbite

How to avoid the hazards: PPE Safety

Gloves

Table 1-1: Safety Labels placed on Product

Illustration of Label Location

6

1. Safety CryoPod Carrier

Fire Hazards Part Number: 257437 Rev. A

Fire Hazards

The CryoPod Carrier provides no direct fire hazard.

Explosion Hazards

Improper Use of liquid nitrogen

Do not place objects on top of the CryoPod Carrier.

Do not overfill with liquid nitrogen.

Do not tightly seal the CryoPod Carrier.

Thermal Hazards

Extreme Temperature

Contact with cold surfaces can cause death or serious injury

from frost bite.

Do not operate the product without all covers in place.

Do not touch components that show signs of frost. Wait for them

to defrost or use insulated gloves or appropriate protective

equipment.

Use extreme care to prevent contact with LN₂ by spilling or

splashing.

7

Brooks Automation 1. Safety

Part Number: 257437 Rev. A Ergonomic Hazards

Chemical Hazards

Asphyxiation

The liquid nitrogen used in this product is non-flammable and

non-toxic. However, as with any gas, death or serious injury due

to asphyxiation could occur if concentrated gas is inhaled.

l When working with refrigerant gases, follow all facility and

local regulatory and environmental procedures regarding

the storage, handling, and disposal of the gases.

l Fill, service, and use the unit in a well ventilated area.

Ergonomic Hazards

Tip Hazard

Do not place product above typical laboratory bench level.

Maximum height of 106.7 cm (42 in) to minimize risk of tipping

or knocking the unit over.

Lift Hazard

This product weighs greater than 9.0 kg (20 lb) but less than

11.3 kg (25 lb). Improper lifting may result in personal injury.

Use appropriate lift techniques when lifting this product.

8

1. Safety CryoPod Carrier

Environmental Information Part Number: 257437 Rev. A

Environmental Information

Noise Emission

The CryoPod Carrier provides no direct noise hazard during operation.

Vibration

The CryoPod Carrier provides no direct vibration hazard during operation. Any vibrations produced

during normal operation are minimal and cause no hazardous conditions.

Decommissioning and Disposal

Brooks Life Science Systems complies with the EU Directive 2002/96/EU Waste Electrical and

Electronic Equipment (WEEE). The obligations for disposal for the product and components are

passed to the end user for responsible disposal. No cost for disposal is included in the initial cost of

the equipment.

For further information and assistance in disposal, please contact the Brooks Life Science Systems

technical support.

9

Brooks Automation 1. Safety

Part Number: 257437 Rev. A Regulatory Compliance and Declaration of Conformity (DOC)

Regulatory Compliance and Declaration of Conformity

(DOC)

The Product meets the requirements of the European Union’s Machinery Directive 2006/42/EC as a

completed machine. In accordance with the Directive, BioCision has issued a Declaration of

Conformity and the Product has a CE mark affixed.

10

2. Overview CryoPod Carrier

Part Number: 257437 Rev. A

2. Overview

The CryoPod™ Carrier is a portable liquid nitrogen (LN₂) based system that, with the CryoPod Carrier

lid secure, provides over four hours of < -150°C temperature stability for the handling and

transportation of cryogenic biospecimens. The system’s battery powered electronic module displays

the cryogenic chamber temperature as well as operator defined parameters such as date, time,

instrument identification, alarms and log function activation.

The included manual fill kit facilitates the system LN₂ charging process utilizing approximately 3 liters

of LN₂. The cryogenic chamber consists of an aluminum basket that is seated above the LN₂

absorbent pad assembly, eliminating contact between the chamber’s biological contents and LN₂.

With a built-in handle and compact dimensions, the CryoPod Carrier can be hand-carried over short

distances such as around a laboratory or research campus to extend the cryogenic cold chain.

11

Brooks Automation 2. Overview

Part Number: 257437 Rev. A Using this Manual

Using this Manual

Failure to review the Safety chapter and follow the safety warnings

can result in death or serious injury.

l All personnel involved with the operation or maintenance

of this product must read and understand the information

in this safety chapter.

l Follow all applicable safety codes of the facility as well as

national an international safety codes.

l Know the facility safety procedures, safety equipment, and

contact information.

l Read and understand each procedure before performing

it.

The CryoPod Carrier is intended for use in a laboratory environment by trained laboratory personnel.

The manuals and related materials are intended for use by trained and experienced technical

personnel.

The manufacturer accepts no liability for any other use of the equipment or its individual parts and

components. This also applies to service and repair work carried out by unauthorized service

personnel. All warranties are declared null and void in the event of non-compliance with these

instructions. This also applies to parts not directly affected by any unauthorized repair work.

This manual contains information on safety, specifications, and operation as well as troubleshooting

and maintenance of the CryoPod Carrier. If there are any questions regarding this manual or use of

this system or to order additional copies of this publication, contact Brooks Automation.

12

2. Overview CryoPod Carrier

Specifications Part Number: 257437 Rev. A

Specifications

Parameter Specifications

Internal Basket dimensions 17.4 x 18.8 x 7.8 cm (6.9 x 7.4 x 3.1 in)

External CryoPod Carrier dimensions 34.0 x 32.0 x 26.0 cm (13.4 x 12.6 x 10.2 in)

Capacity One 2" tall cryobox, two SBS Microplates, or small cassettes

Weight

(without samples)

5.1 kg (11.2 lbs) without LN₂

6.9 kg (15.2 lbs) fully charged with LN₂

LN₂ fill volume (full charge)

~3 L

Operating Environment 15 – 35 °C (59 – 95 °F)

Hold Time at < - 150°C

Lid On: >4 hours

Lid Off: >1 hour

Relative Humidity (RH) 10 - 85% recommended, 30 - 70% ideal

Power Rating 5 watts

Battery Requirements 3 "AA" (LR6) Alkaline (included)

3 “AA” Battery expected life Over 2 years of routine use

Maximum data log hours 100 hours

Display temperature increments 1°C

Temperature acquisition and data

logging rate

60 sec

Display temperature range -199 to 49 °C

Display temperature accuracy ±5°C

Alarms Two programmable temperature alarms

Compliance CE Mark, RoHS, WEEE, FCC, EN 61010, EN 61326

13

Page is loading ...

Brooks CryoPod Carrier User manual

Brooks CryoPod Carrier User manual

Related papers

Other documents