Bio-Tek FL600 User manual

BIO-TEK FL600

FLUORESCENCE

PLATE READER

HARDWARE GUIDE

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HIGHLAND PARK, BOX 998, WINOOSKI, VT 05404-0998 USA.

(800) 451-5172 • (802) 655-4040 • FAX: (802) 655-7941

BIO-TEK FL600

™

FLUORESCENCE

PLATE READER

For Research & Development Use Only

HARDWARE GUIDE

Part No. 6001000

June, 1998

Revision D

Bio-Tek FL600 iii

Notices

BIO-TEK INSTRUMENTS, INC.

Highland Park, Box 998

Winooski, Vermont, USA

05404-0998

Customer Service & Sales: 888-451-5171

Outside U.S.: 802-655-4040

Service: 800-24-BIOTK

Sales FAX: 802-655-7941

Service FAX: 802-655-3399

E-MAIL: [email protected]

[email protected]

Internet: http://www.biotek.com

Lionheart Technologies, Europe N.V.

Sneeuwbeslaan 33

B-2610 Wilrijk

Belgium

TEL: (32) 3-8290455

FAX: (32) 3-8254749

Copyright

publication may be reproduced, transmitted, transcribed, stored in a retrieval

system, or translated into any language without the written permission of Bio-Tek

Instruments, Incorporated.

Trademarks

Bio-Tek

®

is a registered trademark, and FL600™ and KC4™ are trademarks of

Bio-Tek Instruments, Inc.. Windows

®

and Windows 95 are trademarks of the

Microsoft Corporation.

Restrictions and Liabilities

Information in this document is subject to change, and does not represent a

commitment by Bio-Tek Instruments, Inc. Changes made to the information in

this document will be incorporated in new editions of the publication. No

responsibility is assumed by Bio-Tek for the use or reliability of software or

equipment that is not supplied by Bio-Tek, or its affiliated dealers.

Bio-Tek Part No. 6001000

iv Bio-Tek FL600

Revision History

Revision Date Changes

A 4/97 Release to production

B 10/97 Added Stay Clear safety symbol. Corrected Electrical, Optical,

and Performance Specifications sections. Added CE Mark

information. Deleted Appendix A.

C3/98

Preface: Added warranty. Page 2-1: Modified instructions for

Setting Up the FL600 Reader. Page 5-2: Changed electrical

specification for fuses. Page 5-11: Modified text and data on

serial communications to reflect parity differences between KC4

reader software and FL600 diagnostic software. Page 5-13: On

filter table, modified use of excitation filters and added two new

filter wavelengths.

D6/98

Preface: Changed phone numbers and e-mail addresses.

Chapter 4: Changed air filter maintenance procedure. Added

lamp replacement procedure.

Bio-Tek FL600 v

Warranty

This Warranty is limited and applies only to new products, except for computer-based

software which is covered under a separate Warranty Policy, manufactured by

Bio-Tek

®

Instruments, Inc. (“Bio-Tek”). Bio-Tek makes no warranty whatsoever

regarding the condition of used products.

Bio-Tek warrants the instrument (hereinafter collectively referred to as “Products” or

“Product”) for a period of one (1) year from the original purchase date against defective

materials or workmanship. This Warranty is limited to the original purchaser (the

“Purchaser”) and cannot be assigned or transferred. All claims under this Limited

Warranty must be made in writing to Bio-Tek, Attention: Service Department.

Purchaser must ship the Product to Bio-Tek, postage pre-paid. Bio-Tek shall either

repair or replace with new or like-new, at its option and without cost to the Purchaser,

any Product which in Bio-Tek’s sole judgment is defective by reason of defects in the

materials or workmanship.

This Warranty is VOID if the Product has been damaged by accident or misuse, or has

been damaged by abuse or negligence in the operation or maintenance of the Product,

including without limitation unsafe operation, operation by untrained personnel, and

failure to perform routine maintenance. This Warranty is VOID if the Product has been

repaired or altered by persons not authorized by Bio-Tek, or if the Product has had the

serial number altered, effaced, or removed. This Warranty is VOID if any of the

Products has not been connected, installed or adjusted strictly in accordance with

written directions furnished by Bio-Tek. Batteries, fuses, lightbulbs, and other

“consumable” items used in any of the Products are not covered by this Warranty.

Software utilized in conjunction with any of the Products is not covered by the terms of

this Warranty but may be covered under a separate Bio-Tek software warranty.

We will continue to stock parts for a maximum period of five (5) years after the

manufacturer of any equipment has been discontinued. Parts shall include all materials,

charts, instructions, diagrams, and accessories that were furnished with the standard

models.

THIS WARRANTY CONTAINS THE ENTIRE OBLIGATION OF BIO-TEK

INSTRUMENTS, INC., AND NO OTHER WARRANTIES, EXPRESSED, IMPLIED,

OR STATUTORY ARE GIVEN. PURCHASER AGREES TO ASSUME ALL

LIABILITY FOR ANY DAMAGES AND/OR BODILY INJURY OR DEATH

WHICH MAY RESULT FROM THE USE OR MISUSE OF ANY EQUIPMENT OR

INSTRUMENT BY THE PURCHASER, HIS EMPLOYEES, AGENTS, OR

CUSTOMERS, OTHER THAN THE EXPRESS WARRANTY CONTAINED

HEREIN. WE SHALL NOT BE RESPONSIBLE FOR ANY DIRECT OR

CONSEQUENTIAL DAMAGES OF ANY KIND. THIS WARRANTY SHALL NOT

BE CHANGED OR MODIFIED IN ANY WAY WITHOUT THE EXPRESS

WRITTEN PERMISSION OF AN OFFICER OF BIO-TEK INSTRUMENTS, INC.

vi Bio-Tek FL600

Warnings and Precautions

When operated in a safe environment, according to the instructions in this manual,

there are no known hazards associated with the instrument. However, the

operator should be aware of certain situations which could result in serious injury.

Hazard Warnings

Warning! Power Rating. The instrument must be connected to a power

receptacle that provides voltage and current within the specified rating for the

system. Use of an incompatible power receptacle may produce electrical shock

and fire hazards.

Warning! Electrical Grounding. Never use a two-prong plug adapter to

connect primary power to the instrument. Use of a two-prong adapter disconnects

the utility ground, creating a severe shock hazard. Always connect the system

power cord directly to a three-prong receptacle with a functional ground.

Warning! Internal Voltage. Always turn off the power switch and unplug the

power cord before cleaning the outer surfaces of the instrument or changing

filters.

Warning! Liquids. Avoid spilling liquids on the instrument; fluid seepage into

internal components creates a potential shock hazard. Wipe up all spills

immediately. Do not operate the instrument if internal components have been

exposed to fluid. If the microplate appears to have been exposed to fluid, the

reading chamber may also have been exposed. To protect the incubator, remove

the top cover and clean the interior.

Precautions

The following precautions are provided to help you avoid damaging the system:

Caution: Service. The system should be serviced by Bio-Tek-authorized service

personnel. Only qualified technical personnel should perform troubleshooting and

service procedures on internal components.

Caution: Replacement Fuses. Use replacement fuses with the required current

rating and specification. Improper fuses or short-circuiting the fuse holders may

cause fire or damage the instrument.

Bio-Tek FL600 vii

Caution: Environmental Conditions. Do not expose the system to temperature

extremes. System performance may be adversely affected.

Caution: Filter Settings. The software filter settings must match the actual

filters used in the reader, in order to prevent possible damage to the

photomultiplier and/or degradation of data.

Safety Symbols

The following warning and informational symbols may be found in various

locations on the instrument. Only qualified personnel who recognize shock

hazards and are familiar with the safety precautions should use this instrument.

Read the manual carefully before operating this instrument.

Direct current

Courant continue

Gleichstrom

Corriente continua

Corrente continua

Alternating current

Courant alternatif

Dreiphasen-Wechselstrom

Corriente Atterna

Corrente alternata

Both direct and alternating current

Courant continu et courant alternatif

Allstrom (Gleich - und Wechselstrom

Corriente continua y corriente alterna

Corrente continua e corrente alternata

Earth ground terminal

Borne de terre

Erde (Bettriebserde)

Borne de Tierra

Terra (di funzionamento)

Protective conductor terminal

Borne de terre de protection

Schutzleiteranscluss

Borne de Tierra de Protección

Terra di protezione

On (Supply)

Marche (alimentation)

Ein (Verbindung mit dem Netz)

Connectado

Chiuso

viii Bio-Tek FL600

Off (Supply)

Arrest (alimentation)

Aus (Trennung vom Netz)

Desconectado

Aperto (sconnessione dalla rete di alimentazione)

Caution (Refer to accompanying documents)

Attention (voir documents d'accompanement)

Achtung siehe Begleitpapiere

Atención (vease los documentos incluidos)

Attenzione, consultare la doc annessa

Caution, risk of electric shock

Attention, risque de choc electrique

Gefährliche elektrische Spannung

Atención, riesgo de sacudida eléctrica

Alta tensione (in questo documento

Alta tensione non significa "tensione pericolosa"

come definito in IEC 417)

Stay clear

Ne pas toucher svp.

Bitte nicht berühren

No tocario por favor

Si prega di non toccare

Bio-Tek FL600 ix

Contents

1. Introducing the Bio-Tek FL600

Hardware Requirements 1-3

Software Requirements 1-4

2. Getting Started

Setting Up the FL600 Reader 2-1

Installing KC4™ and FL600 Diagnostic Software 2-2

Installing KC4 2-2

Installing the Hardware Key 2-2

Installing the FL600 Diagnostic Software (Diagnostics Use)2-2

Checking the FL600 System 2-4

Using the FL600 Diagnostic Software 2-4

Filter Wavelength Locations 2-5

Checking the COM Port 2-5

Running System Diagnostics 2-7

What to Do if the Tests Fail 2-8

Exiting the FL600 Diagnostic Software 2-9

3. Replacing Filters and Adjusting Probe Height

Adding and Replacing Filters 3-1

Changing the Filters Inside the Reader 3-2

Setting the Filter Positions in KC4 Software 3-6

Utilizing the Filter Adapter System 3-7

Removing the Filter from the Adapter 3-7

Inserting the Replacement Filter into the Adapter 3-8

Adjusting Top Optical Probe Height 3-8

FL600 Interior Configuration Diagrams 3-10

4. Bio-Tek FL600 Maintenance

Maintaining the Reader 4-1

How to Maintain the Exterior 4-1

How to Replace the Air Filter 4-1

How to Replace the Lamp 4-3

x Bio-Tek FL600

5. Technical Information

Bio-Tek FL600 Fluorescence/Absorbance Plate Reader 5-1

Physical Dimensions 5-1

Operating Conditions 5-1

Electrical Specifications 5-2

Optical Specifications 5-2

Scanning Mechanism 5-3

Temperature Control 5-4

Performance Specifications 5-4

CE Mark Approval 5-8

Computer Specifications for FL600 Diagnostic Software 5-10

FL600 Serial Communications 5-11

Ordering Filters 5-12

Fluorescence Filters 5-12

Absorbance Filters 5-14

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This chapter introduces the Bio-Tek FL600 Fluorescence

Plate Reader. You'll find information on hardware and

software requirements and optional equipment

suggestions.

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Thank you for selecting the Bio-Tek FL600. The FL600 is a powerful

fluorescence and absorbance measurement system which measures

through the use of a photomultiplier tube housed inside of a light-

impermeable detection compartment. The FL600 is capable of quantifying

both soluble and cell-associated fluorescence. The system consists of:

• The Bio-Tek FL600 Fluorescence Plate Reader

• KC4™ reader control and data reduction software

• FL600 System Software for diagnostics

• Accessories Kit

• Serial Communications Cable (P/N 75034)

Both software packages are for Windows-based PCs and provides the user

with full command of the operation of the X-Y scanner. FL600’s precision

X-Y mechanism scans a microplate with a sensitive optical detection

system to quantify the fluorescence or absorbance in each well.

Introduction to the Bio-Tek FL600

1-2

Virtually any microplate type from 6 to 384 wells can be used with the

FL600. Users can select any microplate from the FL600’s built-in list of

industry-standard plates, or they can define their own unique plate

format(s).

The FL600 is designed to meet international radio frequency emission

standards, EMC immunity standards, and user safety standards.

The Bio-Tek FL600 should be installed in a well-ventilated area, free from

dust and fumes. Keep the system away from direct sunlight and local heat

sources.

Introduction to the Bio-Tek FL600

1-3

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• IBM

™

486 PC or clone 33 MHz clock

• 640 KB base memory, extended to 8 MB

• Color graphics monitor (VGA)

• Color graphics adapter (VGA)

• 120 MB hard drive

• High-density floppy disk drive 3½"

• 1 serial (COM) and 1 parallel (LPT) port

• Mouse and keyboard ports

• Microsoft or compatible mouse

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• IBM 486 PC or clone 100 MHz clock

• 640 KB base memory, extended to 8 MB

• Color graphics monitor (VGA)

• Color graphics adapter (VGA)

• 120 MB hard drive

• High-density floppy disk drive 3½"

• 1 serial (COM) and 1 parallel (LPT) port

• Mouse and keyboard ports

• Microsoft or compatible mouse

• Surge/spike protector

Introduction to the Bio-Tek FL600

1-4

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• IBM 486 / 100 MHz PC or better compatible

• Memory: 640 KB base, extended to 16 MB minimum

• 250 MB hard drive

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• Microsoft Windows compatible printer

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• FL600 System Software, version 2 or higher

• KC4™ reader control and data reduction software

• MS-DOS version 5.0 or later

• Microsoft Windows version 3.1 or later, or Microsoft

Windows 95 (with 16 MB memory)

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This chapter discusses how to set up the Bio-Tek FL600

hardware, check the communication port and run the

diagnostic tests, install and configure the FL600 software,

and exit the FL600 system software.

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CAUTION: It is recommended that two people lift the FL600, which weighs

about 23

kg (50.6 lb.). Care must be taken when moving the device or removing it from the

packaging. Hold the device firmly from the bottom of the case when lifting it.

Setting up the Bio-Tek FL600 Fluorescence Plate Reader is simply a matter of

connecting the system to the computer via the computer’s serial port, installing

the software, and setting up communications parameters. One of the serial ports

most likely already has a mouse connected to it (the FL600 software, since it

operates under Windows, requires a mouse).

Step 1:

Set the incubator power switch to the appropriate voltage as

follows:

110V for 100-120V˜ operation,

220V for 200-240V˜ operation

Step 2:

Turn off the PC.

Step 3:

To connect the FL600, first attach the 9-pin D-type cable into the

computer’s serial port (COM1, COM2, COM3, or COM4).

Step 4:

Plug the other end of the cable into the Serial Data Port located at

the rear of the FL600 Fluorescence Plate Reader.

Step 5:

Connect the power cable to the back of the Plate Reader.

Step 6:

Plug all power cords into grounded, electrical outlets (use of a surge

protector is strongly recommended).

Getting Started

2-2

The FL600 and all computer hardware must be electrically grounded and

connected to a viable surge protector to avoid dangerous shocks and to

avoid destroying the electronic components through electrical spikes and

surges.

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The FL600 is supplied with two Windows™ software packages, KC4™

and FL600 Diagnostic Software.

KC4 controls the reader, reduces the fluorescence and absorbance data,

and furnishes the reports.

The FL600 Diagnostic software provides diagnostics to test the reader.

Although it contains other functions, these are undocumented and

unsupported.

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Please consult your KC4™ User’s Guide. Place disk 1 into the floppy

drive. Run Setup.exe by choosing the Run option from the File Menu of

your Windows program. This routine will guide you through the

installation of KC4 onto your hard disk.

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To use KC4 to control the reader and print data, the hardware key needs to

be installed on the parallel port of your computer. Please consult your

KC4 Operations manual for further instruction.

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To install the FL600 software, follow these steps:

Step 1:

Turn on your computer, start Windows, and activate Windows’

Program Manager, if necessary.

Step 2:

Insert the FL600 Setup diskette into the floppy drive.

Step 3:

Select

Run

from the Windows’

File

menu.

Step 4:

Type drive: setup, (i.e., b: setup).

Getting Started

2-3

Setup will indicate to you that all FL600 diagnostic software files will be

installed into the drive and directory C:\FL600. You will be given the

opportunity to change either the drive or the directory (or both). Setup

creates a new group called Bio-Tek FL600. Note that this is different from

the KC4™ group created by the KC4™ software.

NOTE: Be sure that TrueType Fonts are enabled in the Fonts Section of the

Windows Control Panel. FL600 requires certain TrueType fonts when

printing reports. For more information on activating TrueType Fonts,

please check your Windows User’s Guide.

Getting Started

2-4

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Before you begin to use the FL600 software, you should first check that

the FL600 settings are correct.

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Step 1:

To start the FL600 diagnostic software:

From within Windows, double-click on the FL600 icon.

Depending on your computer speed, the FL600 diagnostic

software should boot up from within 15 to 60 seconds.

Longer than that might indicate that your computer system

needs more RAM or clock speed.

The FL600 Plate Layout screen will appear:

Figure 2-1: FL600 Plate Layout Screen

Getting Started

2-5

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Refer to Chapter 3 for instructions on determining filter locations. Record

the filter locations to be entered into KC4.

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Next, turn on the FL600 Reader using the switch located on the right side

of the front panel and check the COM Port Setting.

NOTE: When the Reader has been switched on, a green light next the switch

lights up and the plate transport moves out, ready to load a plate.

Step 1:

Pull down the

Instrument

menu and select

Communications

. A

dialog box appears showing you the currently selected

communications port, through which the software is attempting to

communicate with the FL600 Reader:

Figure 2-3: Communications Port Settings Screen

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Bio-Tek FL600 User manual

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