3M Petrifilm™ Environmental Listeria Plates Operating instructions

Petrifilm™ Environmental Listeria Plates
3M
Brand
3M
Model
Petrifilm™ Environmental Listeria Plates
Type
Operating instructions

3M Petrifilm™ Environmental Listeria Plates are sample-ready culture mediums designed to detect the majority of environmental Listeria, including Listeria monocytogenes, Listeria innocua, and Listeria welshimeri. They feature a cold-water-soluble gelling agent and a chromogenic indicator that facilitates easy detection of Listeria colonies. The plates can be used for quantitative, semi-quantitative, or qualitative testing, and provide results in 28-30 hours of incubation.

3M Petrifilm™ Environmental Listeria Plates are sample-ready culture mediums designed to detect the majority of environmental Listeria, including Listeria monocytogenes, Listeria innocua, and Listeria welshimeri. They feature a cold-water-soluble gelling agent and a chromogenic indicator that facilitates easy detection of Listeria colonies. The plates can be used for quantitative, semi-quantitative, or qualitative testing, and provide results in 28-30 hours of incubation.

1
Listeria



Listeria

Listeria

Interpretation
Guide
EL
Listeria
2
Environmental Listeria Plate
Listeria


• Quantitative Interpretation: Listeria


Listeria
• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria
Listeria


• Quantitative Interpretation: Listeria

• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria
Figure 2
Figure 1
ListeriaListeria innocua
Listeria monocytogenesListeria
ListeriaListeria monocytogenesListeria innocuaListeria
welshimeriL. ivanovii, L. grayi/murrayi and L. seeligeri 

Listeria
Listeria
3

are not



remain

Listeria
• Quantitative Interpretation: Listeria

• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria

changed

Listeria
• Quantitative Interpretation: Listeria

• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria
Figure 3b
Figure 3a
Several factors inuence the rate at which the chromogenic indicator changes to intense
red-violet, including the strain and the nature and degree of stress to which the organism
has been exposed.
4
Environmental Listeria Plate
Listeria





• Quantitative Interpretation:Listeria

Listeria





• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria




Listeria


• Quantitative Interpretation: Listeria

• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria
Figure 5
Figure 4
5
1
4





Listeria
• Quantitative Interpretation: Listeria

• Semi-Quantitative Interpretation: Listeria




• Qualitative Interpretation: Listeria
Figure 6
Reminders for Use
Storage Sample Preparation
Inoculation
5















Listeria


≤8°C
2


To prevent exposure to moisture, do
not refrigerate opened pouches.


3








6
Listeria

3
3
3
3
Environmental Listeria Plate
Incubation Interpretation
The 3M Petrilm Environmental Listeria Plate method can be used as a
quantitative, semi-quantitative or qualitative test.
10
13
11
14
12
15




 Please refer
to product instructions for third party
validated methods.
semi-quantitative
relative number






Listeria




Listeria






qualitative
detectednot detected





quantitative






Listeria

Optional




 
Listeria
9
7 8







Listeria



1 42 3

















Listeria

Listeria







Listeria
Quantitative Interpretation
Example: 3M Quick Swab Contact Method


Environmental quantitative sampling is consistent with the following references:
• 
th

• 
5
th


 





 G
Line G equals CFU/area
Quantitative Sampling & Interpretation
 














Environmental Listeria Plate




3M.com/foodsafety/Petrilm

1 42 3















Listeria

3
User’s Responsibilities:









3M Food Safety
3M Center, Building 275-5W-05
St. Paul, MN 55144-1000 USA
1-800-328-6553
3M.com/foodsafety
3M Canada
Post Oce Box 5757
London, Ontario N6A 4T1
Canada
1-800-364-3577
 
 3
 1
 
 
 
  G
Line G equals CFU/area
 
 3
 5
 
 
 
  G
Line G equals CFU/area
Example: Sponge Contact Method
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3M Petrifilm™ Environmental Listeria Plates Operating instructions

Petrifilm™ Environmental Listeria Plates
3M
Brand
3M
Model
Petrifilm™ Environmental Listeria Plates
Type
Operating instructions

3M Petrifilm™ Environmental Listeria Plates are sample-ready culture mediums designed to detect the majority of environmental Listeria, including Listeria monocytogenes, Listeria innocua, and Listeria welshimeri. They feature a cold-water-soluble gelling agent and a chromogenic indicator that facilitates easy detection of Listeria colonies. The plates can be used for quantitative, semi-quantitative, or qualitative testing, and provide results in 28-30 hours of incubation.

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