Thermo Fisher Scientific VitroEase Negative Stains User guide

Brand
Thermo Fisher Scientific
Model
VitroEase Negative Stains
Type
User guide
VitroEase™ Negative Stains
Catalog Numbers A51036 and A51037
Doc. Part No. 2162744 Pub. No. MAN0025329 Rev. A.0
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and
gloves. Safety Data Sheets (SDSs) are available from thermofisher.com/support.
Product description
VitroEase™ Methylamine Tungstate Negative Stain is a ready-to-use stain with excellent spreading qualities with high density for good contrast. It
wets both grid films and specimens well at neutral pH. This stain does not damage delicate structures as much as phosphotungstate (PTA) and
has been found to be useful for negative staining of macromolecules, viruses, and membranes.
VitroEase™ Methylamine Vanadate Negative Stain is a ready-to-use stain with excellent spreading properties at a near-neutral pH of 8. It produces
a light, uniform negative stain for high-resolution, fine-structure visualization and does not denature protein samples. This stain is also stable and
non-volatile in the electron beam, making it more resistant than uranyl acetate negative stain.
Contents and storage
Product Catalog Number Storage Temperature
VitroEase™ Methylamine Tungstate Negative Stain A51036 4°C
VitroEase™ Methylamine Vanadate Negative Stain A51037
Required materials not supplied
• Lab film
• Whatman filter paper
Note: Avoid complete drying of the grid during the procedure.
Perform negative staining
1. Place two 50-µL drops of deionized water and two 50-µL of stain on a piece of lab film.
2. Apply 4 µL of sample to a freshly glow-discharged EM grid covered with a continuous carbon film and incubate for 1 minute. Blot the grid
from the side with a piece of filter paper.
3. Briefly touch the grid on the first drop of deionized water and blot the edge of the grid gently with filter paper. Repeat this step.
4. Briefly touch the grid on the first drop of stain and blot the edge of the grid with filter paper.
5. Touch the grid on the second drop of stain and incubate for 50–60 seconds.
6. Completely blot dry the grid by touching only the rim with filter paper.
7. Air dry the grid for >5 minutes prior to loading on the microscope.
8. Clean the tweezers with ethanol.
Limited product warranty
Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of
Sale at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at
www.thermofisher.com/support.
Thermo Fisher Scientific | 3747 N. Meridian Road | Rockford, Illinois 61101 USA
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all
applicable Limited Use Label Licenses.
©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
  • Page 1 1

Thermo Fisher Scientific VitroEase Negative Stains User guide

Brand
Thermo Fisher Scientific
Model
VitroEase Negative Stains
Type
User guide
Download PDF
report

Ask a question and I''ll find the answer in the document

Finding information in a document is now easier with AI

Related papers