Lovibond 56B006101 Owner's manual

www.lovibond.com

Legionella pneumophila serogroup 1 in Water

Instruction Manual

Part Number: 56B006101

Systems Test Kit

Lovibond

Water Testing

Tintometer

Group

Overview

The Legionella Systems Test Kit is for detecting Legionella in a water sample -- it is ideal for on-line and a variety

of industrial applications.

The test operates via a Lateral Flow Immuno-Chromatographic Assay (LFICA).

Each Systems Test Kit contains the following:

 •5Individuallyfoil-wrappedtests--eachcontainsanexactvolumepipette

 •5HollowFiberFilters

 •5SyringeswithRecoveryBuffer

 •1SampleCollectionBottle

 •1Samplepointpipeadapter

 •Instructionsforuse

This test is intended for analysis of water samples only. This product is not intended for clinical or medical

diagnostic use.

Theproductisintendedtobeusedaspartofanoverallwatertreatment,management,andriskreductionap-

proachandshouldNOTbeusedasthesolemethodforassessingrisksassociatedwithLegionellabacteria.

Test Operating Limits

Thetesthasbeenevaluatedforoperationbetween10-40ºC(50-104ºF).

Thetesthasbeenvalidatedforsamplesthatlterinlessthan10minutes.Samplesrequiringmorethan10min-

utestoltermaygiveerroneousresults.Samplesthatrequirelongtimestosamplemaybeindicativeofpoor

systemmaintenance.Awiderangeofnon-oxidizingbiocidesandbiodispersantshavebeencheckedforcross

reaction and inference with the test. This test should not be used on systems treated with biguanide or

THPS based biocides!

PerformanceofthetestwithsamplesotherthanthosetakenfromCoolingTowers,DomesticHot&Coldwater

systemsandWhirlpoolspashasnotbeenestablished.

Disposal

Thetest,lter,syringeandcapscannotbereusedorrecycled.Thepackagingmaterialsandthisinstruction

manualcanberecycled.

Storage

Thetestisintendedforstorageatroomtemperature.DoNotFreeze.Whenstoredcorrectly,thetestwill

continuetooperatewithindesignspecications,untilthespeciedexpirationdate.Do not use the test after

the stated expiration date specied on the packaging, Do not use any test where the foil packaging

is damaged.

Technical Information

Limit of Detection

Laboratoryanalysishasdemonstratedthat98-100%(91%Cl)oftestsarepositiveforcleanwatersamples

containing100CFU/LiterLegionella pneumophilaserogroup1.Thetheoreticalmathematicallimitofdetec-

tionofthetestisequivalent100CFU/Lwhena250mlsampleisltered.Ifsmallervolumesareprocessed,the

detectionlimitwillbealteredaccordingly:

Suspendedsolidcontentinthewatersamplesimpactsltrationandtestperformance,includinganalyticalsen-

sitivity.Acutualresultswillvary.Watersampleswithhighlevelsofsuspenedsolidsmayblockltrationentirely.

Legionella pneumophilaserogroup1bacteriarecoveryfromwatersamplescanrangefrom<10to100%,

dependingonwatersamplecomposition.Thisissimilartoltrationconcentrationtechniquestusedinother

microbiologicalanalysismethods.

limit of detection =

100CFU

Sample Volume (L)

Fit Sample Point

Identifythewatersystemtobesampledandanappropriatelocationfromwhichtoobtainarepresentative

sample.Largesystemsmayneedtobesampledandtestedatmultiplelocations.Attachthesamplepoint

adaptertothepointinthesystemtobetested.Itisrecommendedthatasamplepointadapterbettedand

retained to each sample point to avoid cross contamination. The sample line pressure should not exceed 6

bar (87 psi).

Resuspend the Bacteria

Disconnectthelterfromthesamplepointadapterbytwistingthelockringandpullingthelterfromthet-

ting.Removethewhitescrewcapfromthelterandreplaceitontheoppositeendofthelter.

 •Pullthesyringebacktothe0.5mlmarktosuspendtherecoverybuffer,thenpushthesyringeall

 thewaytothe0mlmark.

 •Repeatthisprocesstwo(2)additionaltimes.

 •Drawthesyringebacktocollect0.1mlofsample,thendisconnectfromthelter.Thesyringe

 nowconainstherecoveredbacteriareadyfortesting.

Add Sample to the Test Strip

Removethetestfromitsfoilwrapping,andplacethetestonaatsurface.

Thefoilwrappingshouldnotbeopeneduntilimmediatelypriortorunningthetest.Ifthefoilisopenedand

thenthetestisnotperformedwithin60minutes,discardthetest.

Beforeuse,thetestshouldhave2palebluelinesacrosstheresultwindow.Ifthesearenotpresent,notifyyour

suppliertoreplacethetestkit.Takethepipettefromthefoilwrapping.

Placethesyringeoverthesmallsamplewindowattheendoftheteststrip,andpresstheplungersothat0.1ml

of sample is dispensed onto the test strip. Record the time.

Read the Test Strip

Leavetheteststripsittingonaatsurfaceduringincubation.Foroptimumresultsthetestresultsshouldbe

performed at room temperature. After waiting 25 minutes, examine the test strip in good lighting. If the

testisnotreadwithin60minutesofaddingthewatersample,itshouldbediscardedandanewtestshouldbe

run. The test should show one of the following results on the large result window on the test strip:

Negative Result:One(1)REDlineacrosstheresultwindowattheendfurthestfromthesamplewindow.

Positive Result:Two(2)REDlinesacrosstheresultwindow.Theredlineclosesttothesamplewindowmay

beveryfaint(palepink).Anydistinctline,nomatterhowfaint,shouldbecountedaspositive.

Invalid Result: If the test does not show any red lines; or if it only shows a line at the end closest to the sample

window;orifthelinefurthestfromthesamplewindowisveryfaint,thenthetestisinvalid.Repeatthetestand

notify your supplier for technical support.

Test Procedure

Take the Sample

Beforetakingthesample,ushthesampleportfor15seconds.Attachtheltertothesamplepointadapter;

pushtheopenendofthelterintotheplasticluerttingandtwistthelockingringtosecure.Holdthesample

collectionbottleundertheoutletportofthelter.

Openthesystemvalvetoallowtheltrationtostart,lter250mlofthewaterandcloseoffthevalve.

Ifthesampletakeslongerthan10minutestolter,thenstoptheltrationprocessandmeasuretheamountof

liquidinthecollectionbottlebeforeproceedingtothenextstep(thedetectionlimitwillbeaffected,seLimitof

Detectionsection).

Tintometer Inc.

6456ParklandDrive

Sarasota,FL34243

Phone:941.756.6410

Fax:941.727.9654

Email: sales@tintomter.us

Web:www.lovibond.com

Interpreting the Results

Positive Results:

A positive test result indicates that Legionella pneumophilaserogroup1bacteriawerepresentinthesample

abovethedetectionlimit.

Thetestdoesnotdifferentiatebetweenviable(living)andnon-viable(dead)organisms.Thetestwilldetectvi-

ablebutnon-cultruablebacteriawhicharenotdetectablebytraditionallaboratorytechniques.Apositiveresult

doesnotnecessarilymeanthatviablebacteriaarepresent.

Whenapositiveresultisobserved,seekadvicefromyourriskmanagementplan,orwatertreatmentspecialist.

Negative Results:

A negative test result indicates that Legionella pneumophilaserogroup1bacteriawerenotdetectedorthe

numberofbacteriainthesamplewerebelowthedetectionlimit.

A negative result does not necessarily mean that Legionella bacteriaareabsent.

AnegativeresultdoesnotmeanthatthesystemiscompletelyfreefromrisksassociatedwithLegionella bacte-

ria. The test only detects Legionella pneumophilaserogroup1.Thetestdoesnotdetectthepresenceofother

Legionella species or serogroups.

Test Procedure, continued.

Specicity

The test has been shown to be non-reactive with the following bacteria ( at 1 x 10

organisms per sample):

Staphylococcus aureus and Legionella pneumophilaserogroups4&7inconcentrationshigherthan1x10

organisms per sample may interfere with test results in negative samples. These concentrations are higher than

woudlbeexpectedtobepresentinnormalwatersamples.

Acinetobacter calcoaceticus,

Aeromonas hydrophila subsp.

hydrophila, Bacillus subtilis,

Burkholderia cepacia, Citrobacter

freudii, Citrobacter koseri,

Escherichia coli, Enterobacter

cloacae, Klebsiella oxytoca, Pseu-

domonas

aeruginosa, Pseudomonas

uorescens, Pseudomonas putida,

Pseudomonas stutzeri,

Ralstonia pickettii, Raoultella

terrigena, Streptococcus pyro-

genes, Yersinia ruckeri

HYDROSENSEisatrademarkofAlbagaiaLimited,usedworldwideandregisteredinvariousterritories.

Lovibond 56B006101 Owner's manual

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