Accu-Scope EXC-500 Gout Kit Operating instructions

Accu-Scope
Brand
Accu-Scope
Model
EXC-500 Gout Kit
Type
Operating instructions
73 Mall Drive, Commack, NY 11725 ● PH (631) 864-1000 ● F (631) 543-8900 ● info@accu-scope.com ● www.accu-scope.com
EXC-500 Microscope Series
Gout Kit Installation
Gout Kit Components
Fig. 1
Installation
Adjust the the EXC-500 for Kohler illumination with the objective
of your choice.
Polarizer (Fig. 1)
Install the polarizer onto the light port so its thumb screw faces
toward the 3 O’clock position. This will set the orientation to
East-West.
Thumb Screw
(for Analyzer)
Polarizer
Analyzer
73 Mall Drive, Commack, NY 11725 ● PH (631) 864-1000 ● F (631) 543-8900 ● info@accu-scope.com ● www.accu-scope.com
Fig. 2
Installation (continued)
Analyzer Dust Plug (Fig. 2)
Remove the dust plug from the slot above the nosepiece.
Analyzer (Fig. 3 & 4)
Slide the analyzer into the slot above the nosepiece as shown.
It will automatically be in the North-South orientation. (Fig. 3)
Use the thumb screw provided to secure the analyzer. (Fig. 4)
Fig. 3
Fig. 4
Using the Gout Kit
A full wave retarder filter is
incorporated into the Gout Kit
polarizer (Fig. 1). The retarder can
be rotated 90°. This enables
distinction between true gout and
pseudo-gout crystals.
Monosodium urate crystals are
elongated prisms that have a
negative birefringence.
The crystals will exhibit a yellow
interference color when the crystals
long axis is orientated parallel to the
slow axis of the full wave retarder
45° degrees to the crossed
polarizers).
If the crystals are “true-gout”,
rotating the Polarizer (P1) will
change the interference color to
blue.
If the crystals are “Pseudo-gout”
(pyrophosphate) the crystals will be
blue when parallel to the slow axis of
the full wave plate, changing to blue
when P1 is rotated 90 degrees (from
East-West to North-South).
Monosodium urate crystals grow in
elongated prisms that have a
negative optical sign of birefringence
which generates a yellow
(subtraction) interference color when
the long axis of the crystal is
oriented parallel to the slow axis of
the first order retardation plate.
Rotating the crystals through 90°
changes the interference color to
blue (addition color). In contrast,
pseudo-gout pyrophosphate
cyrstals, which have similar
elongated growth characteristics,
exhibit a blue interference color
when oriented parallel to the slow
axis of the retardation plate and a
yellow color when perpendicular.
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Accu-Scope EXC-500 Gout Kit Operating instructions

Accu-Scope
Brand
Accu-Scope
Model
EXC-500 Gout Kit
Type
Operating instructions
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