CMA BT29i User guide

CMA
Brand
CMA
Model
BT29i
Type
User guide
COLORIMETER(BT29i!
USER’S!GUIDE!
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CENTRE FOR MICROCOMPUTER APPLICATIONS
http://www.cma-science.nl
"!|!#$"%&!'()(*&+,-,*!./,*0/!12&3,!!
Short!description!
$4,! '56! '()(*&+,-,*! #$"%&! +,7/2*,/! -4,! 7+(28-! (9! )&:4-! -*78/+&--,3! -4*(2:4! 7!
/7+;),!/()2-&(8!783!&-!<78!=,!2/,3!-(!3,-,*+&8,!-4,!<(8<,8-*7-&(8!(9!7!/()2-&(8>!?-!
47/!9(2*!@AB!)&:4-!/(2*<,/!,+&--&8:!)&:4-!(9!3&99,*,8-!C7D,),8: -4/ E!D&( ),-!FGH!8+E!=)2,!
FIH!8+E!:*,,8!JKJ!8+!783!*,3!KGJ!8+>!$4,!C7D,),8:-4!&/!/,),<-,3!=L!;*,//&8:!-4,!
9*(8-! ;78,)! 7**(C! M,L/>! $4,! '()(*&+,-,*! &/! 3,)&D,*,3! C&-4! NH! ;)7/-&<! <2D,--,/! C&-4!
<7;/>!!
!
$4,! '()(*&+,-,*! <78! =,! 3&*,<-)L! <(88,<-,3! -(! -4,! 787)(:! #$! &8;2-/! (9! -4,! '56!
&8-,*97<,/>! $4,! /,8/( *! <7=),! #$! O! ?AAANG%F ! 8,,3,3! -(! <(88,<-! -4,! /,8/(*! -(! 78!
&8-,*97<,!&/!8(-!/2;; )&,3 !C&-4!-4,!/,8/(*!78 3!47/!-(!=,!;2*<4 7 /,3 !/,;7*7-,)L!P' 5 6!
6*-&<),!#$/<QNR>!!
Sensor!recognition!
$4,! '()(*&+,-,*! #$"%&! 47/! 7! +,+(*L! <4&;! PAASTU5R! C&-4! &89(*+7-&(8! 7=(2-! -4,!
/,8/(*V! &-/!87+,E! +,7/2 *,3!W278-&-LE! 28&-!783!<7)&=*7-&(8>!$4*(2:4!7!/&+; ),!;*(-(<()!
-4&/! &89(*+7-&(8! &/! *,73! =L! -4,! '56! &8-,*97<,/! 783! -4,! /,8/(*! &/! 72-(+7-&<7))L!
*,<(:8&X,3! C4,8! &-! &/! <( 88 ,< -,3 ! -(! -4 ,/ ,! &8-,*97<,/>! ! ?9! L(2*! '()(*&+,-,*! &/! 8(-!
72-(+7-&<7))L!3,-,<-,3!=L!78!&8-,*97<,!L(2!47D,!-(!+7827))L!/,-!2;!L(2*!/,8/(*!=L!
/,),<-&8:!&-!9*(+!-4,!'(7<4!Y,8/(*!@&=*7*L>!!
How!the!Colorimeter!works!!
@&:4-!9*(+!-4,! @AB!;7//,/!-4*(2:4!7!<2D,--,!-47-!<(8-7&8/! 7!/7+;),!/()2-&(8>!6!)&:4-O
/,8/&-&D,! ;4(-(<,))! 7-! -4,! (-4,*! ,83! (9! -4,! )&:4-! ;7-4! 3,-,<-/! -4,! 7+ (2 8-! (9!
-*78/+&--,3!)&:4->!!!
!
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!
!
!
!
!
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!
!
!
@&:4-!3,-,<-(*!
'2D,--,!
/7+;),!
@AB!@&:4-!/(2*<,!
2D,--,!/7+;),!
#$"%&!'()(*&+,-,*!./,*0/!12&3,! |!G!
$4,! ;*(;(*-&(8! (9! -4,! )&:4-! -47-! ;7//,/! -4*( 2: 4! -4,! /()2-&(8! &/! M8(C8! 7/!
Transmittance!T!783!&/!,Z;*,//,3! 7/!-4,!*7-&(!(9!-4,!&8-,8/&-L!(9!-4,!-*78/+&--,3! )&:4-!
?
-E!
783!-4,!&8&-&7)!&8-,8/&-L!(9!-4,!)&:4-!=,7+!?
H
V!$[!?
-!
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H
!
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!
$4,! *,<&;*(<7)! (9! -*78/+&--78<,! (9! -4,! /7+;),! D7*&,/! )(:7*&-4+&<7))L! C&-4! -4*,,!
97<-(*/V!-4,!+()7*!7= /( *; -&D&-L!(9!-4,!/()2-&(8!ε,!-4,!<,))!(*!<2D,--,!C&3-4!=E!783!-4,!
+()7*!<(8<,8-*7-&(8!'V!!
log(
1
T
) = ε∗b∗C
!
578L! ,Z;,*&+,8-/! 3,/&:8,3! -(! 2/,! 7! '()(*&+,-,*! *,W2&*,! 7! +,7/2*,+,8-! (9!
Absorbance!AE!C4&<4!&/!3,9&8,3!7/V!
A = log(
1
T
)
!
!(*!
A = ε∗b∗C
!
!
](*! 7! :&D,8! /()2-&(8! <(8-7&8,3! &8! 7! <2D,--,! C&-4! 7! <(8/-78-! <,))! C&3-4E! (8,! <78!
7//2+,!ε 783!=!-(!=,!<(8/-78->!$4&/!),7 3/ !-(!-4, !,W2 7-&( 8 V!
A = k ∗C
E!C4,*,!M!&/!7!;*(;(*-&(87)&-L!<(8/-78->!
!
$4&/!,W27-&(8!/4(C/!7=/(*=78<,!-(!=,!*,)7-,3!3&*,<-)L!-(!<( 8<,8-*7-&(8!P#,,*0/!)7C R!
783!/(!7=/(*=78<,!<78!=,!2/,3!-(!+,7/2*,!-4,!<(8<,8 -*7-&( 8!( 9!7!/( )2-&( 8 >!!
!
$*78/+&--78<,! <78! =,! 7)/(! ,Z;*,//,3! 7/! Percent! Transmittance! (*! ^$>! Y&8<,!
$[^$\NHHE! -4,! 9(*+2)7! 3,/<*&=&8:! -4,! *,)7-&(8/4&;! =,-C,,8! 7=/(*=78<,! 783!
-*78/+&--78<,!<78!=,!*,C*&--,8!7/V!
A = log(100 / %T)
!!
(*!!
>
!
!
$4,! )&8,7*!*,)7-&(8/4&;!=,-C,,8!6=/(*=78<,!783!'(8<,8-*7-&(8!3(,/!8(-!4()3!7<*(//!
-4,! C4(),! $*78/+&--78<,! *78:,>! ](*! =,/-! *,/2)-/! (2*! -,/-&8:! (9! -4,! '()(*&+,-,*!
&83&<7-,/!-47-!-*78/+ &--78 <,!( *!7= /( *= 78 <, !D7)2 ,/ !/4 ( 2)3 !=,!C&-4&8!-4,/,!*78:,/V!
• $*78/+&--78<,V!NH^!O!%H^!!
• 6=/(*=78<,V!H>HJ!_!N>H>!
AZ;,*&+,8-/! 2/&8:! -4,! 7=/(*=78<,! *78:,! /4(2)3! =,! 3,/&:8,3! -(! 9&-! C&-4&8! -4,/,!
D7)2,/>!?9!L( 2 !47D,!7!/()2-&(8 !-47-!-*78/+&-/!D,*L!)(C!),D,)!(9!)&: 4-E!<(8/&3,*!3&)2-&8:!
-4,!/()2-&(8!/(!-47-!&-!97))/!C&-4 &8!-4 &/!*78 :,>!
!
!
!
!
!
F!|!#$"%&!'()(*&+,-,*!./,*0/!12&3,!!
Calibration!!
$4,!'56!'()(*&+,-,*!#$"%&!&/!/2 ; ; )&,3 !<7)&=*7-,3>!$4,!(2-;2-!(9!-4,!'()(*&+,-,*!&/!
)&8,7*!C&-4!*,/;,<-!-(!-4,!;,*<,8-!-*78/+&--78<,>!$4,!/2; ;)&,3!<7)&=*7-&(8!928<-&(8!&/!
+73,!9(*!6=/(*=78<,!6[!H>JFF!_!H>FGF`)8a
(2-
PaR
!N
>!
$4,!'(7<4!/(9-C7*,!7))(C/!/,),<-&8:!-4,!<7)&=*7-&(8!/2;;)&,3!=L!-4,!/,8/(*!+,+(*L!
PAASTU5R!(*!-4,!<7)&=*7-&(8!/-(*,3!&8!-4,!'(7<4!K!Y,8 /(*!@&=*7 *L>!!
!
#,9(*,! <()),<-&8:! 37-7! -4,! X,*(O;(&8-! <7)&=*7-&(8!
9(*!-4,!/,),<-,3!C7D,),8:-4 !/4(2)3 !=,!;,*9(*+,3>!
$(!;,*9(*+!/2<4!<7)&=*7-&(8V!!
• '(88,<-!-4,!'()(*&+,-,*!-(!78!&8-,*97<,>!!
• @,-! -4,! /L/-,+! /-7=&)&X,! 7-! -4,! 3,/&*,3!
C7D,),8:-4! 9(*! J! +&82-,/
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>! U8,! (9! -4,! 9(2*!
:*,,8!C7D,),8:-4! &83&<7-(*!)&:4-/! &/!-2*8,3 ! (8!
C4,8!-4,!'()(*&+,-,*!&/!;(C,*,3>!
• S*,//!-4,! !(*! !=2--(8!(8!-4,!'()(*&+,-,*!
-(! /,),<-! -4,! <(**,<-! C7D,),8:-4! /,--&8:! 9(*!
L(2*!,Z;,*&+,8-!PFGH!8+E!FIH!8+E!JKJ!8+E!(*!KGJ!8+R>!!
• U;,8!-4,!'()(*&+,-,*!)&3>!
• ]&))!7!<2D,--,!C&-4!3&/-&)),3!C7-,*>!$4&/!C&))!=,!L(2*!=)78M!+,7/2*,+,8-!PNHH!^!
-*78/+&--78<,!(*!H!7=/(*= 78 <,R>!
• ?8/,*-!-4,!/7+;),!<2D,--,!&8-(!-4,!<2D,--,!4()3,*>!!?-!&/!&+;(*-78-!-(!)&8,!2;!(8,!
(9!-4,!/+((-4!/&3,/!(9!-4,!<2D,--,!7-!-4,!-(;!(9!-4,!<2D,--,!/)(->!b(-&<,!-47-!-4,*,!
<78!=,!/)&:4-!3&99,*,8<,!&8!-*78/+&//&(8!&9!-4,!<2D,--,!&/!*(-7-,3!=L!NcH!3,:*,,/>!
• ')(/,!-4,!'()(*&+,-,*!)&3>!!
• b,Z-E!;*,//!-4,! !=2--(8!-(!=,:&8!-4,!<7)&=*7-&(8!;*(<,//>!T,),7/,!-4,!=2--(8!
C4,8!-4,!*,3!@AB!=,:&8/!-(!9)7/4>!$4,!<7)&=*7-&(8!&/!9&8&/4,3!C4,8!-4,!@AB!/-(;/!
9)7/4&8:!783!-4,!+,7/2*,3!7=/(*=78<,!C&))!=,!H>HHH!(*!H>HHN>!$4,!28&-!&/!8(C!
*,73L!-(!<()),<-!37-7>!
Collecting!data!!
69-,*!-4,!X,*(O;(&8-!<7)&=*7-&(8!&/!9&8&/4,3!L(2!<78!/-7*-!L(2*!+,7/2*,+,8 -V!!
• ]&))!7!<2D,--,!C&-4!">"! -( !G>J!+@!(9!-4,!/()2-&(8!L(2! C78-!-(!+,7/2*,!P-4,!D()2+,!
(9!-4,!<2D,--,!&/!F!+ @R>!')(/, !&-!C&-4 !-4 ,!<7 ;>!b(-&<,!-47-!C&-4!/(+,!, Z; ,*&+,8-/!
P9(*!,Z7+ ;),!*7-,!(9!*,7<-&(8R!-4,!-&+,!-7 M,8!&8!9&--&8:!-4,!<7;!<78!*,/2)-!&8!)(//!(9!
,7*)L!37-7>!
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
N
!'7)<2)7-,3!7/!9())(C&8:!6[!"!_!)(:P^$R[!"!_!)( :P " c>J I N ` a
(2-
R[H>JFF!_!)(:Pa
(2-
R[!H>JFF!_!H>FGF`)8Pa
(2-
R!
2
$](*!=,/-!*,/2)-/E!-4,!/L/-,+!/4(2)3!=,!/-7=&)&X,3!9(*!J!+&82-,/!;*&(*!-(!<7)&=*7-&(8!(*!37-7!<()),<-&(8>!!
#$"%&!'()(*&+,-,*!./,*0/!12&3,! |!J!
• T,;)7<,! -4,! =)78M! <2 D,--,! &8! -4,! '()(*&+,-,*! C&-4! -4,! <2D,--,! <(8-7&8&8:! -4,!
/()2-&(8>!d783),!-4,!<2D,--,!=L!-4,!<7;!783!7D(&3!-(2<4&8:!&-!C&-4!L(2*!9&8:,*/>!!
• ')(/,!-4,!'()(*&+,-,*!<7;!783!/-7*-!L(2*!37-7!<()),<-&(8>!!
Wavelength!selection!!
$4,!C7D,),8:-4!&/!/,),<-,3!=L!;*,//&8:! !(*! !=2--(8/!(8!-4,!9*(8 -!;78,)>!$4,*,!
7*,!/,D,*7)!C7L/!(8,!<78!3,<&3,!C4&<4!(9!-4,!9(2*!)&:4-!C7D,),8:-4/!-(!2/,>!
!
Method!1!
@((M!7-!-4,!<()(*!(9!-4,!/()2-&(8>!T,+,+=,*!-47-!-4,!<()(*!(9!7!/()2-&(8!&/!-4,!<()(*!
(9!)&:4-!-47-!;7//,/!-4*(2:4!&->!e(2!;*(=7=)L!C78-!-(!2/,!7!3&99,*,8-!<()(*!(9!)&:4-!-47-!
C&))!=,!7=/(*=,3E!*7-4,*!-478!-*78/+&--,3f!9(*!,Z7+;),E!C&-4!7!=)2,!<(;;,*!P??R!/2)97-,!
P'2YUFR!/()2-&(8E!2/,!-4,!*,3!@AB!PKGJ!8+R>!
!
Method!2!
68(-4,*!,7/L!+,-4(3!&/!-(!;)7<,!7!<2D,--,!<(8-7&8&8:!-4,!/()2-&(8!&8!W2,/-&(8!&8!-4,!
'()(*&+,-,*! 783! <4,<M! -(! /,,! C4&<4! (9! -4,! C7D,),8:-4/! L&,)3/! -4,! 4&:4,/-!
7=/(*=78<,>!
!
Method!3!
B&*,<-&(8/! 9(*! +(/-! <()(*&+,-*L! ,Z;,*&+,8-/! ,Z;*,//! 7! *,<(++,83,3! C7D,),8:-4>!
./,!-4,!C7D,),8:-4! <)(/,/-!&8!D7)2,! -(! -4,!*,<(++,83,3!C7D,),8:-4>! AD,8!&9!-4,!
@AB!C7D,),8:-4!&/!/(+,C47-!3&99,*,8-E!7!#,,*0/! )7C!<2*D,!<78!2/2 7))L!=,!(=-7&8,3!7-!
7)+(/-!78L!C7D,),8:-4!&8!-4,!D&<&8&-L!(9!-4,!*, <( + + ,8 3 ,3 !C7 D,), 8: -4 >!!
Using!cuvettes!with!the!Colorimeter!!
$4,! '()(*&+,-,*! &/! 3,/&:8,3! -(! 2/,! /-7837*3! 3&/;(/7=),!
;()L/-L*,8,! <2D,--,/> 57<*(! <2D,--,/! 47D,! 7! NH! ++! ;7-4!
),8:-4!783!7*,!FJ!++!4&:4>! $4,!-(-7)!D()2+,! (9!7!<2D,--,!&/!F!
+@>!$4,! <2D,--,! 47/!-C(!/+((-4! 783! -C(! *&==,3!97<,/>!$4,!
/+((-4! 97<,/! 7*,! -4,! (;-&<7)! /2*97<,/! &8-,83,3! -(! -*78/+&-!
-4,!)&:4-!9*(+!-4, !@AB>!?-!&/!&+;(*-78-!-(!;( /&- &(8 !-4,!<2D,--,!
<(**,<-)L! &8! -4,! '()(*&+,-,*E! C&-4! 7! /+((-4! /&3,! 97<&8:! -4,!
-(;!783!-4,!=(--(+!(9!-4,!<2D,--,!/)(-E!783!C&-4!-4, !*&==,3!
,3:,/!97<&8:!),9-!783!*&:4->!!
!
?83&D&327)!;)7/-&<!<2D,--,/!D7*L!/)&:4-)L!&8!-4,!7+(28-!(9!)&:4-!
-4,L! 7= /( *=>! ](*! +(/-! ,Z;,*&+,8 -/E! -4&/! D7*&7-&( 8 ! C&))! 8(-!
47D,! 7!8(-&<,7=),! ,99,<-!(8! ,Z;,*&+,8-7)!*,/2)-/!783!L(2!+7L!
<4((/,!-(!&:8(*,!-4,/,!3&99,*,8<,/>!](*!=,/-!*,/2)-/E!D7*&7-&(8!
&8!)&:4-!7=/(*=,3!=L!&83&D&327)!<2D,--,/!<7 8!=,!<(8-*()),3!,&-4,*!=L!2/&8:!-4,!/ 7+ ,!
<2D,--,!9(*!7))!-*&7)/!(9!7!;7*-&<2)7*!,Z;,*&+,8-!(*!=L!matching!7!/,-!(9!<2D,--,/>!!
$(;!
D&,C!
K!|!#$"%&!'()(*&+,-,*!./,*0/!12&3,!!
57-<4,3! <2D,--,/! 7*,! 7! /,-! (9! <2D,--,/! -47-! 7))! 7=/(*=! )&:4-! PC4,8! ,+;-LR! 7-!
7;;*(Z&+7-,)L!-4,!/7+,! ),D,)>!$(!+7-<4!7!/,-!(9!<2D,--,/! 2/,!<),78E!3*L!<2D,--,/E!;2-!
7!*,9,*,8<,!+7*M!(8!(8,!(9!-4,!<),7*!/&3,/! ( 9! -4,!<2D,--,/!/(!&-!&/!7)C7L/!(*&,8-,3! -4,!
/7+,! C7L! &8! -4,! <2D,--,! /)(->! S)7<,! ,7<4! <2D,--,! &8! -4,! '()(*&+,-,*! 783! *,<(*3!
7=/(*=78<,!D7)2,/!9(*!,7<4>!g4,8!L(2!7*,!9&8&/4,3!:*(2; !<2D,--,/!7<<(*3&8:!-(!-4,!
/&+&)7*!7=/(*=78<,!D7)2,/>!!
!
Suggested!experiments!!
$4,!'()(*&+,-,*!<78!=,!2/,3!&8!,Z;,*&+,8-/!/2<4!7/V!!!
• 6;;)&<7-&(8!(9!#,,*h/!)7C!,>:>!'*L/-7)!D&(),-!(*!'(;;,*!Y2);47-,>!
• 5,7/2*&8:!(9!28M8(C8!<(8<,8-*7-&(8/>!
• T,7<-&(8!M&8,-&</!_!+,7/2*&8:!*,7<-&(8!*7-,E!*,7<-&(8!(*3,*!(*!*,7<-&(8!
,W2&)&=*&2+>!
• i278-&-7-&D,!3,-,*+&87-&(8!(9!=&()(:&<7)!+(),<2),/!,>:>!/2:7*/E!;*(-,&8E!D&-7+&8/>!
• i278-&-7-&D,!3,-,*+&87-&(8!(9!&8(*:78&<!&(8/!,>:>!8&-*7-,E!;4(/;47-,>!
• S(;2)7-&(8!:*(C-4!(9!+ &<*( ( *:7 8&/ + />!!
Practical!information!
• B(!8(-!),-!)&W2&3/!,8-,*!-4,!=(3L!(9!-4,!'()(*&+,-,*>!!
• B(!8(-!2/,!(*:78&<!<(+;(283/!9*(+!-4,!7*(+7-&<E!47)(:,87-,3E!7)&;47-&<E!M,-(8,E!
7)3,4L3,!(*!,/-,*!:*(2;/!&8!-4,!;()L/-L*,8,!<2D,--,/>!
!
#$"%&!'()(*&+,-,*!./,*0/!12&3,! |!I!
Technical!Specifications!
!
Sensor!kind!
687)(:E!:,8,*7-,/!78!(2-;2-!D()-7:,!=,-C,,8!0 - 5 V !
Useful!!
Measurement!range!
S,*<,8-!$*78/+&--78<,V!%H^!>>!NH^!
6=/(*=78<,V!!H>HJ!>>!N>H!!
Wavelengths!ranges!
a&(),-E!FGH!8+!(*!FGHH!j!
#)2,E!FIH!8+!(*!FIHH!j!
1*,,8E!JKJ!8+!(*!JKJH!j!
T,3E!KGJ!8+!(*!KGJH!j!
Resolution!using!!
12!bit!AD!converter!
!
H>H"J!^$!
Supply!voltage!!
J!aB'!k"J!+a!
Supply!current!(typical)!!
FH!+6!
Calibration!functions!
6[!H>JFF!_!H>FGF`)8a
(2-
PaR!P/-(*,3!&8!-4,!/,8/(*!+,+(*LR!
^$![!"c>JIN!`!a
(2-
PaR!
Connection!
?AAANG%F!<(88, <-( *!9 (*!# $ O?A A A NG %F!/,8/(*!<7=),>!!
Y,8/(*!<7=),!8(-!3,)&D,*,3!C&-4!-4,!/,8/(*>!!
!
!
!
!
!
!
Warranty:!!
$4,! '()(*&+,-,*! #$"%&! &/! C7**78-,3! -( ! =,! 9*,,! 9*(+! 3,9,<-/! &8! +7 -,*&7)/ ! 783!
C(*M+78/4&;!9(*!7!;,*&(3!(9!N"!+(8-4/!9*(+!-4,!37-,!(9!;2*<47/,!;*(D&3,3!-47-!&-!
47/!=,,8!2/,3!283,*!8(*+7)!)7=(*7-(*L!<(83&-&(8/>!$4&/!C7**78-L!3(,/!8(-!7;;)L!&9!
-4,!/,8/(*!47/!=,,8!3 7+ 7: ,3 !=L!7<<&3 ,8 -!(*!+&/2/,>!!
!
Note:!This!product!is!to!be!used!for!educational!purposes!only.!It!is!not!appropriate!
for!industrial,!medical,!research,!or!commerc ial!app licatio ns.! !! ! ! !
Rev. 20/11/2014
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CMA BT29i User guide

CMA
Brand
CMA
Model
BT29i
Type
User guide
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